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The map label for this gene is hemA

Identifier: 16752326

GI number: 16752326

Start: 40478

End: 41497

Strand: Direct

Name: hemA

Synonym: CP0032

Alternate gene names: 16752326

Gene position: 40478-41497 (Clockwise)

Preceding gene: 16752316

Following gene: 16752327

Centisome position: 3.29

GC content: 39.41

Gene sequence:

>1020_bases
ATGGTGTTAGGAGTTGTTGGAATCAGTTATCGTGAAGCCGCTTTGAAAGAAAGAGAGCGTGCGATTCAATATCTGCAATC
TTTTGAAAAAAATCTTTTCCTTGCACAGCGTTTTTTAGGCAAAGGAGGAGCGTTTATCCCGTTACTTACTTGTCATCGAG
CCGAACTTTATTATTATTCAGAAAGTCCTGAAATTGCTCAAGCCGCCTTACTTTCAGAATTGACATCCCAGGGAATACGT
CCTTACCGTCATAGAGGTTTGTCTTGTTTTACTCATCTGTTTCAGGTCACCAGTGGTATAGATAGTTTGATCTTTGGGGA
AACGGAAATTCAAGGACAGGTAAAACGAGCCTATTTAAAAGGAAGCAAAGAGAGAGAGTTGCCTTTTGATCTTCATTTTT
TATTTCAAAAAGCTCTAAAGGAAGGGAAGGAGTATCGCTCACGCATCGGGTTTCCCGATCATCAAGTGACCATAGAATCT
GTCGTTCAAGAAATTCTACTCAGCTACGACAAGTCGATATATACGAATTTTTTATTCGTAGGATACTCGGATATCAATAG
GAAAGTCGCTGCCTACTTATATCAACATGGGTATCATAGGATTACCTTTTGCTCTAGACAACAAGTCACGGCCCCCTATA
GAACCTTGTCTCGAGAAACGCTCTCATTTCGACAACCCTATGATGTGATTTTCTTTGGCTCTTCAGAATCAGCTTCACAA
TTTTCCGATCTTTCCTGTGAGAGTCTTGCTAGCATTCCCAAACGTATTGTCTTTGATTTTAACGTGCCACGAACCTTTTT
ATGGAAAGAAACCCCTACAGGTTTTGTTTATTTAGATATAGATTTCATCAGTGAATGCGTGCAAAAAAGACTGCAATGCA
CCAAAGAAGGAGTAAATAAAGCAAAACTTTTGTTAACTTGTGCCGCGAAAAAACAATGGGAAATTTATGAAAAAAAGAGC
TCACATATAACCCAGAGGCAAATTTCATCCCCACGTATTCCTTCCGTTCTAAGCTATTGA

Upstream 100 bases:

>100_bases
GCTTCTAATTTTCTCTTCGTCTATACAGCTAAGTTACTTTTCATTTATAAATTTAGTATAAGTTTCCTTCATACTGTTCT
GAAATTATCGAATCGTGCTT

Downstream 100 bases:

>100_bases
TGCTTTAATGCCTAATTCATCGCTTGACATATACAGCTTTCGAAAATACTCTAAGCTTTCATTAGAAGTACGAATATCCT
CTGATGTCTGTGAGGGAATG

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MVLGVVGISYREAALKERERAIQYLQSFEKNLFLAQRFLGKGGAFIPLLTCHRAELYYYSESPEIAQAALLSELTSQGIR
PYRHRGLSCFTHLFQVTSGIDSLIFGETEIQGQVKRAYLKGSKERELPFDLHFLFQKALKEGKEYRSRIGFPDHQVTIES
VVQEILLSYDKSIYTNFLFVGYSDINRKVAAYLYQHGYHRITFCSRQQVTAPYRTLSRETLSFRQPYDVIFFGSSESASQ
FSDLSCESLASIPKRIVFDFNVPRTFLWKETPTGFVYLDIDFISECVQKRLQCTKEGVNKAKLLLTCAAKKQWEIYEKKS
SHITQRQISSPRIPSVLSY

Sequences:

>Translated_339_residues
MVLGVVGISYREAALKERERAIQYLQSFEKNLFLAQRFLGKGGAFIPLLTCHRAELYYYSESPEIAQAALLSELTSQGIR
PYRHRGLSCFTHLFQVTSGIDSLIFGETEIQGQVKRAYLKGSKERELPFDLHFLFQKALKEGKEYRSRIGFPDHQVTIES
VVQEILLSYDKSIYTNFLFVGYSDINRKVAAYLYQHGYHRITFCSRQQVTAPYRTLSRETLSFRQPYDVIFFGSSESASQ
FSDLSCESLASIPKRIVFDFNVPRTFLWKETPTGFVYLDIDFISECVQKRLQCTKEGVNKAKLLLTCAAKKQWEIYEKKS
SHITQRQISSPRIPSVLSY
>Mature_339_residues
MVLGVVGISYREAALKERERAIQYLQSFEKNLFLAQRFLGKGGAFIPLLTCHRAELYYYSESPEIAQAALLSELTSQGIR
PYRHRGLSCFTHLFQVTSGIDSLIFGETEIQGQVKRAYLKGSKERELPFDLHFLFQKALKEGKEYRSRIGFPDHQVTIES
VVQEILLSYDKSIYTNFLFVGYSDINRKVAAYLYQHGYHRITFCSRQQVTAPYRTLSRETLSFRQPYDVIFFGSSESASQ
FSDLSCESLASIPKRIVFDFNVPRTFLWKETPTGFVYLDIDFISECVQKRLQCTKEGVNKAKLLLTCAAKKQWEIYEKKS
SHITQRQISSPRIPSVLSY

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=217, Percent_Identity=29.0322580645161, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_CHLPN (Q9Z7J1)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   B72043
- PIR:   G86579
- RefSeq:   NP_224910.1
- RefSeq:   NP_300770.1
- RefSeq:   NP_444584.1
- RefSeq:   NP_877013.1
- ProteinModelPortal:   Q9Z7J1
- GeneID:   1467420
- GeneID:   895228
- GeneID:   919483
- GeneID:   963654
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0032
- KEGG:   cpn:CPn0714
- KEGG:   cpt:CpB0741
- TIGR:   CP_0032
- HOGENOM:   HBG413932
- OMA:   IFFGSSE
- ProtClustDB:   PRK00676
- BioCyc:   CPNE115711:CP_0032-MONOMER
- BioCyc:   CPNE115713:CPN0714-MONOMER
- BioCyc:   CPNE138677:CPJ0714-MONOMER
- BioCyc:   CPNE182082:CPB0741-MONOMER
- BRENDA:   1.2.1.70
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015895
- InterPro:   IPR018214

Pfam domain/function: PF05201 GlutR_N; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 39104; Mature: 39104

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 51-51 BINDING 102-102 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLGVVGISYREAALKERERAIQYLQSFEKNLFLAQRFLGKGGAFIPLLTCHRAELYYYS
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHEEECEEEEEEC
ESPEIAQAALLSELTSQGIRPYRHRGLSCFTHLFQVTSGIDSLIFGETEIQGQVKRAYLK
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHC
GSKERELPFDLHFLFQKALKEGKEYRSRIGFPDHQVTIESVVQEILLSYDKSIYTNFLFV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHEEEE
GYSDINRKVAAYLYQHGYHRITFCSRQQVTAPYRTLSRETLSFRQPYDVIFFGSSESASQ
EHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHH
FSDLSCESLASIPKRIVFDFNVPRTFLWKETPTGFVYLDIDFISECVQKRLQCTKEGVNK
HHCCCHHHHHHCHHHEEEECCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCH
AKLLLTCAAKKQWEIYEKKSSHITQRQISSPRIPSVLSY
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCC
>Mature Secondary Structure
MVLGVVGISYREAALKERERAIQYLQSFEKNLFLAQRFLGKGGAFIPLLTCHRAELYYYS
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHEEECEEEEEEC
ESPEIAQAALLSELTSQGIRPYRHRGLSCFTHLFQVTSGIDSLIFGETEIQGQVKRAYLK
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHC
GSKERELPFDLHFLFQKALKEGKEYRSRIGFPDHQVTIESVVQEILLSYDKSIYTNFLFV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHEEEE
GYSDINRKVAAYLYQHGYHRITFCSRQQVTAPYRTLSRETLSFRQPYDVIFFGSSESASQ
EHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHH
FSDLSCESLASIPKRIVFDFNVPRTFLWKETPTGFVYLDIDFISECVQKRLQCTKEGVNK
HHCCCHHHHHHCHHHEEEECCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCH
AKLLLTCAAKKQWEIYEKKSSHITQRQISSPRIPSVLSY
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362