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Identifier: 16752202

GI number: 16752202

Start: 1124485

End: 1125723

Strand: Direct

Name: Not Available

Synonym: CP1033

Alternate gene names: 16752202

Gene position: 1124485-1125723 (Clockwise)

Preceding gene: 16752201

Following gene: 16752203

Centisome position: 91.43

GC content: 39.55

Gene sequence:

>1239_bases
ATGAAAAAAAACGCATCTCATAAAACAAATGATAAAAAAAGTTTGTCCATTTGGTCTATTGGAGGATCTATTTTTGCTAT
GTTCTTCGGAGCTGGCAATATTGTCTTTCCCTTAGCTCTGGGATACCACTACAATGCGCATCCCTGGTCTGCCTATTTCG
GCATGATGCTTACTGCTGTATGCGTTCCTCTTTTAGGTCTAGTCAGTATGCTATTCTATTCTGGAGACTACCAGAAGTTC
TTTTTCTCGATAGGAAGAATTCCAGGGATGATTTTTATTACGGCTATTATACTTTTGATAGGCCCCTTCGGTGGAATCCC
TCGAGCCATTGCAGTATCTCATGCCACATTGATTTCACTATCCGAGCATAAGAGTGCTTTCATTCCCTCTTTGCCTATTT
TTAGTGCGATTTGCTGTGTGCTTATCTATATCTTCTCATGCAAGCTCAGCCGTTTAATTCAATGGCTAGGATCTGTATTT
TTCCCGATTATGTTGGTTACCTTACTTTGGGTCATCATCCGCAGTTTTATGATTCCTACCCACCCCATGGTTCAAGAATT
TATTCCGAACGCACGTCAAGCTTGGTTGGCAGGATTCATTGAAGGGTTCAATACTATGGATTTACTTGCAGCGTTTTTCT
TCTGCTCCATAGTTTTGATCTCTCTACGTCAGCTCGTAGCTGAAGAGAAACATCCTACAGAGGAAGAAATCCCTCTTAGT
TTTCAAGGTATTAGCAAAAAAAATAAGCGTTCCTTAGCTTTAGGTTTCTTTCTTGCTGCGATCTTACTAGGAATGACCTA
TCTTGGATTTGTTTTATCTGCTGCACGTCATGCGGGCTTACTTGTGAATGTAAGTAAAGGTCATATTCTGGGAAGGATCT
CTGCGATTGCATTAGGGCCAAATAGCATTCTCGCAGGAGTCAGCGTTTTCATTGCCTGTCTAACTACGGAAATTGCTTTA
GTAGGTATTGTTGCAGATTTTCTAGCTCGTGTAGTTTCCTTTAAGAAACTGAACTATGCTTCAGCAGTAATTTGTACTCT
TATCCCCACCTATTTAATTTCTATTTTGAATTTTGAAACTATAAGTCACCTACTCTTGCCTTTGCTACAACTTAGCTATC
CAGCTTTGATTGTACTGGCTTGTGGAAACATTGCCTACAAGTTATGGAATTTCCGATACTCCCCAGTCTTGTTTTATTTA
ACCCTCTCTCTTACAATTGTTTTAAAGTTAGTGAATTAA

Upstream 100 bases:

>100_bases
GTACAATAAAAACTAACTACTGAAAATCGTTTGATTTCTAAGATCATGATTCTTTAAACTCATTCACTCAGCACTTTACT
ATCTCTACTTTATTTAAAAG

Downstream 100 bases:

>100_bases
AATAACATACGGGTTGCTAGCATAATATTATGGTTTTAGAAGCATTAGCCATCTTTCGACAGGATGCGATGCAACATCTC
CTTAAACATCGCAAGGAGAT

Product: branched-chain amino acid transport system carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKKNASHKTNDKKSLSIWSIGGSIFAMFFGAGNIVFPLALGYHYNAHPWSAYFGMMLTAVCVPLLGLVSMLFYSGDYQKF
FFSIGRIPGMIFITAIILLIGPFGGIPRAIAVSHATLISLSEHKSAFIPSLPIFSAICCVLIYIFSCKLSRLIQWLGSVF
FPIMLVTLLWVIIRSFMIPTHPMVQEFIPNARQAWLAGFIEGFNTMDLLAAFFFCSIVLISLRQLVAEEKHPTEEEIPLS
FQGISKKNKRSLALGFFLAAILLGMTYLGFVLSAARHAGLLVNVSKGHILGRISAIALGPNSILAGVSVFIACLTTEIAL
VGIVADFLARVVSFKKLNYASAVICTLIPTYLISILNFETISHLLLPLLQLSYPALIVLACGNIAYKLWNFRYSPVLFYL
TLSLTIVLKLVN

Sequences:

>Translated_412_residues
MKKNASHKTNDKKSLSIWSIGGSIFAMFFGAGNIVFPLALGYHYNAHPWSAYFGMMLTAVCVPLLGLVSMLFYSGDYQKF
FFSIGRIPGMIFITAIILLIGPFGGIPRAIAVSHATLISLSEHKSAFIPSLPIFSAICCVLIYIFSCKLSRLIQWLGSVF
FPIMLVTLLWVIIRSFMIPTHPMVQEFIPNARQAWLAGFIEGFNTMDLLAAFFFCSIVLISLRQLVAEEKHPTEEEIPLS
FQGISKKNKRSLALGFFLAAILLGMTYLGFVLSAARHAGLLVNVSKGHILGRISAIALGPNSILAGVSVFIACLTTEIAL
VGIVADFLARVVSFKKLNYASAVICTLIPTYLISILNFETISHLLLPLLQLSYPALIVLACGNIAYKLWNFRYSPVLFYL
TLSLTIVLKLVN
>Mature_412_residues
MKKNASHKTNDKKSLSIWSIGGSIFAMFFGAGNIVFPLALGYHYNAHPWSAYFGMMLTAVCVPLLGLVSMLFYSGDYQKF
FFSIGRIPGMIFITAIILLIGPFGGIPRAIAVSHATLISLSEHKSAFIPSLPIFSAICCVLIYIFSCKLSRLIQWLGSVF
FPIMLVTLLWVIIRSFMIPTHPMVQEFIPNARQAWLAGFIEGFNTMDLLAAFFFCSIVLISLRQLVAEEKHPTEEEIPLS
FQGISKKNKRSLALGFFLAAILLGMTYLGFVLSAARHAGLLVNVSKGHILGRISAIALGPNSILAGVSVFIACLTTEIAL
VGIVADFLARVVSFKKLNYASAVICTLIPTYLISILNFETISHLLLPLLQLSYPALIVLACGNIAYKLWNFRYSPVLFYL
TLSLTIVLKLVN

Specific function: Component of the LIV-II transport system for branched- chain amino acids. This LIV-II transport system may be H(+)- coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=383, Percent_Identity=26.6318537859008, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45350; Mature: 45350

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCHHHEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHCCHHHEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]