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The map label for this gene is tyrS [H]

Identifier: 167040949

GI number: 167040949

Start: 2378648

End: 2379868

Strand: Reverse

Name: tyrS [H]

Synonym: Teth514_2338

Alternate gene names: 167040949

Gene position: 2379868-2378648 (Counterclockwise)

Preceding gene: 167040951

Following gene: 167040948

Centisome position: 96.85

GC content: 36.12

Gene sequence:

>1221_bases
GTGTCTGTACTTGATGTTTTACAAGAAAGAGGATATATTCAGCAAATGACTCATGAAGAGGAAATAAGAGAGCTTTTAGA
GAAGGAAAAAATCACATTTTACATAGGTTTTGACCCTACTGCAGACAGTTTGCATGTAGGACATTTTCTTCAAATTATGG
TAATGTCTCACATGCAAAAAGCAGGCCACAGGCCTATTGTGTTAATAGGCGGCGGCACAGCCATGGTAGGAGACCCAAGC
GGCAGGACTGATATGAGGAAGATATTAACTAGAGAGGAAATAAATAGAAATGCTGAAGCTTTTAAAAAGCAAATGCAAAG
GCTTATCGATTTCTCTGATGGCAAAGCCATCATGGCCAATAATGCTGATTGGCTTTTAAACTTAAATTATATTGAATTTT
TGCGAGATATAGGGGTTCACTTCTCTGTAAATAGAATGCTTACAGCAGAATGCTTTAAGTCCCGATTAGAAAGAGGTCTT
TCCTTTTTAGAATTTAACTACATGCTGATGCAAGCTTATGATTTTCTTGAATTAAATAAAAGATACAATTGTGTAATGCA
AATGGGCGGAGACGATCAATGGTCTAATATTATTGCTGGAATAGAGCTTATACGAAAAAAAGAAGGCAAACAGGCTTATG
GCATGACATTTACTTTACTTACCACCAGCGAAGGGAAGAAAATGGGAAAAACAGAAAAAGGAGCAGTGTGGCTTGATCCG
AATAAGACTTCTCCTTATGAATTCTATCAATATTGGAGGAATATAGCTGATGCGGATGTGGAAAAGGCTCTGGCACTTCT
CACTTTCTTGCCTATGGATGAAGTAAGAAGATTAGGCGCATTGAAAGATAAAGAGATAAACGAGGCGAAAAAAGTATTGG
CATATGAGGTTACAAAACTTATACACGGAGAGGAAGAGGCTATAAAAGCTCAAAAGGCGGCAGAGGCTTTATTTGAAAGC
GGTGGAGATTTAGATAATGTGCCTTCTGTTACTGTCTCTTCTGATGTTTTAGGTAAAAAAATTTTAGATGTTCTCTTTGA
AGCAAAGATAATACCTTCAAAGAGCGAAGGAAGAAGGCTTATTCAACAAGGCGGGCTATATATAAACAATGAAAAAGTTG
AAAGTGTTGATGAATATATAAAAGATAATATGATAAAAGATGATAGCATTTTAATAAGAAAAGGCAAAAAAGAGTATCAC
AGGCTTGTTATTAAAGAATAA

Upstream 100 bases:

>100_bases
TCAATATTATAAAGTGATAAAATTATATTGTCAAAAATATTTGAAAATATGCTTTTCATTTGATACAATATACAAAAATA
AAAGCAATGGAGGTATTATC

Downstream 100 bases:

>100_bases
AGGGGGAGCTTTTTGTGTCAAGAGAATTTCTCTTGGCACAAATTTTATATGTTCTTGAAAGAGGTGCTAAGATGGTCTTT
ATCGCAACCGCTTTATATAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSVLDVLQERGYIQQMTHEEEIRELLEKEKITFYIGFDPTADSLHVGHFLQIMVMSHMQKAGHRPIVLIGGGTAMVGDPS
GRTDMRKILTREEINRNAEAFKKQMQRLIDFSDGKAIMANNADWLLNLNYIEFLRDIGVHFSVNRMLTAECFKSRLERGL
SFLEFNYMLMQAYDFLELNKRYNCVMQMGGDDQWSNIIAGIELIRKKEGKQAYGMTFTLLTTSEGKKMGKTEKGAVWLDP
NKTSPYEFYQYWRNIADADVEKALALLTFLPMDEVRRLGALKDKEINEAKKVLAYEVTKLIHGEEEAIKAQKAAEALFES
GGDLDNVPSVTVSSDVLGKKILDVLFEAKIIPSKSEGRRLIQQGGLYINNEKVESVDEYIKDNMIKDDSILIRKGKKEYH
RLVIKE

Sequences:

>Translated_406_residues
MSVLDVLQERGYIQQMTHEEEIRELLEKEKITFYIGFDPTADSLHVGHFLQIMVMSHMQKAGHRPIVLIGGGTAMVGDPS
GRTDMRKILTREEINRNAEAFKKQMQRLIDFSDGKAIMANNADWLLNLNYIEFLRDIGVHFSVNRMLTAECFKSRLERGL
SFLEFNYMLMQAYDFLELNKRYNCVMQMGGDDQWSNIIAGIELIRKKEGKQAYGMTFTLLTTSEGKKMGKTEKGAVWLDP
NKTSPYEFYQYWRNIADADVEKALALLTFLPMDEVRRLGALKDKEINEAKKVLAYEVTKLIHGEEEAIKAQKAAEALFES
GGDLDNVPSVTVSSDVLGKKILDVLFEAKIIPSKSEGRRLIQQGGLYINNEKVESVDEYIKDNMIKDDSILIRKGKKEYH
RLVIKE
>Mature_405_residues
SVLDVLQERGYIQQMTHEEEIRELLEKEKITFYIGFDPTADSLHVGHFLQIMVMSHMQKAGHRPIVLIGGGTAMVGDPSG
RTDMRKILTREEINRNAEAFKKQMQRLIDFSDGKAIMANNADWLLNLNYIEFLRDIGVHFSVNRMLTAECFKSRLERGLS
FLEFNYMLMQAYDFLELNKRYNCVMQMGGDDQWSNIIAGIELIRKKEGKQAYGMTFTLLTTSEGKKMGKTEKGAVWLDPN
KTSPYEFYQYWRNIADADVEKALALLTFLPMDEVRRLGALKDKEINEAKKVLAYEVTKLIHGEEEAIKAQKAAEALFESG
GDLDNVPSVTVSSDVLGKKILDVLFEAKIIPSKSEGRRLIQQGGLYINNEKVESVDEYIKDNMIKDDSILIRKGKKEYHR
LVIKE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=411, Percent_Identity=37.2262773722628, Blast_Score=257, Evalue=1e-68,
Organism=Escherichia coli, GI1787925, Length=421, Percent_Identity=42.7553444180523, Blast_Score=347, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI25145802, Length=427, Percent_Identity=33.7236533957845, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI25145805, Length=326, Percent_Identity=33.1288343558282, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6325160, Length=435, Percent_Identity=33.7931034482759, Blast_Score=237, Evalue=3e-63,
Organism=Drosophila melanogaster, GI20130383, Length=431, Percent_Identity=37.5870069605568, Blast_Score=299, Evalue=3e-81,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46472; Mature: 46341

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLDVLQERGYIQQMTHEEEIRELLEKEKITFYIGFDPTADSLHVGHFLQIMVMSHMQK
CCHHHHHHHCCHHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AGHRPIVLIGGGTAMVGDPSGRTDMRKILTREEINRNAEAFKKQMQRLIDFSDGKAIMAN
CCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEC
NADWLLNLNYIEFLRDIGVHFSVNRMLTAECFKSRLERGLSFLEFNYMLMQAYDFLELNK
CCCEEEECHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
RYNCVMQMGGDDQWSNIIAGIELIRKKEGKQAYGMTFTLLTTSEGKKMGKTEKGAVWLDP
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCEECCEEEEEEEECCCCCCCCCCCCEEEECC
NKTSPYEFYQYWRNIADADVEKALALLTFLPMDEVRRLGALKDKEINEAKKVLAYEVTKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
IHGEEEAIKAQKAAEALFESGGDLDNVPSVTVSSDVLGKKILDVLFEAKIIPSKSEGRRL
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCHHHHHH
IQQGGLYINNEKVESVDEYIKDNMIKDDSILIRKGKKEYHRLVIKE
HHHCCEEEECHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHCCCC
>Mature Secondary Structure 
SVLDVLQERGYIQQMTHEEEIRELLEKEKITFYIGFDPTADSLHVGHFLQIMVMSHMQK
CHHHHHHHCCHHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AGHRPIVLIGGGTAMVGDPSGRTDMRKILTREEINRNAEAFKKQMQRLIDFSDGKAIMAN
CCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEC
NADWLLNLNYIEFLRDIGVHFSVNRMLTAECFKSRLERGLSFLEFNYMLMQAYDFLELNK
CCCEEEECHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
RYNCVMQMGGDDQWSNIIAGIELIRKKEGKQAYGMTFTLLTTSEGKKMGKTEKGAVWLDP
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCEECCEEEEEEEECCCCCCCCCCCCEEEECC
NKTSPYEFYQYWRNIADADVEKALALLTFLPMDEVRRLGALKDKEINEAKKVLAYEVTKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
IHGEEEAIKAQKAAEALFESGGDLDNVPSVTVSSDVLGKKILDVLFEAKIIPSKSEGRRL
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCCHHHHHH
IQQGGLYINNEKVESVDEYIKDNMIKDDSILIRKGKKEYHRLVIKE
HHHCCEEEECHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]