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The map label for this gene is nadE [H]

Identifier: 167040910

GI number: 167040910

Start: 2307471

End: 2309102

Strand: Direct

Name: nadE [H]

Synonym: Teth514_2296

Alternate gene names: 167040910

Gene position: 2307471-2309102 (Clockwise)

Preceding gene: 167040909

Following gene: 167040911

Centisome position: 93.9

GC content: 33.33

Gene sequence:

>1632_bases
ATGAAAATTGCATTGGCACAAATAAATCCAGTTGTAGGAGATATAAAAGGAAACTTCGAAAAGATAGTAGAGTTTATAAA
TACAGCAAAATACCAAAAAGCTGACCTTGTGGTGTTCCCTGAACTAGCTACTGTAGGTTATCCTCCTAAAGACTTTTTGT
TTATGCAAGATTTTTTAGAGGCCAATGAAAAATATATAAATGAAATAGTCTTGCCCAATACTAATCAAATTGGAGTAATA
CTCGGTACGGTAAGACAAGACACAGAGGGCAACCTTTATAATTCTGCTTTTTTTATTTATGAGGGTGAAATAGTCGAGAT
TTTTGATAAGACTTTACTTCCAAACTATGATGTATTTGATGAAAAAAGGTATTTTAAACCAGCACAATTAAGAAAGGTCG
CTTCTTTTAAAGGGATAAAATTAGGAGTTAATATATGTGAAGACATATGGAAAGATTATGTTTTTGAACCAAATGTAGAT
TATTCTGTTGATGTTTTAGAAGAACAATTTAAGCTTAAACCTGATATTTTTATAAATATTTCAGCATCTCCTTACTACCT
GGGTAAGGAAAATATGAGAGTAGAAATGATAGAGAAAAAAATAAAAAAGTACGCAATTCCTTTTGTTTACGTGAACCAAG
TTGGTGCAAATGATGAATTGATATTTGACGGTGCTAGCTTTGTAGTAAATGAAGAAGGCAAAAGAGTGGTACAACTTAAA
GCTTTTGAGGAAGATATTAAAGTTTTCGATATAGATGAACTGAAAAATCTTAGGAAACTGCCCGAAATAAAAGAAGATAT
ATCATGGGTATACAATGCTCTTGTCCTTGGTGTAAGAGATTATTGTAAAAAATCAGGTTTTAAAAAGGCAGTAGTAGGCT
TAAGCGGTGGAATAGATTCTGCAGTAGTTTGTGCCATTGCAGTAGAAGCTTTAGGAAAAGAAAATGTTCTTGCAGTATCA
ATGCCTTCAAGATATTCCTCTGAAGGAAGCAAAAGTGATGCAAGAATACTGGCTGATAATTTGGGAATTGAATTTAGAGT
AATCCCAATAGAGCCTGTGTTCAAAAGTTATTTATCCGTTTTCAATGGGGATAACAACGTTATAGGTGACCTGGCAGAAG
AAAACATACAGGCAAGAATAAGAGGTAATTATTTAATGTTTATATCAAATAGGGAAGGGCACATTGTTTTAACTACAGGA
AATAAATCAGAATTGGCAATGGGATATTGCACTCTTTACGGAGATATGAGCGGAAGCCTTGCACCAATTGCAGATGTACC
TAAGACAATGGTATACGATCTGGCGAGGTTTATAAACAGAGAAAGAGAAATAATTCCAATTTCTATAATTGAAAAAGCTC
CTTCTGCAGAATTAAGACCTAATCAAAAAGATGAAGACTCATTGCCTCCTTACAAGATTTTGGATGAAATATTAAAAATG
TATATAGAGGAAAACAGGTCAGCAAAAGAAATAATAGAAAAAGGATATGATGAAAGATTGGTAAAAGATGTTATAAATAA
AGTCAACAGAGTAGAGTACAAAAGAAAACAAGCAGCACCTGGACTCAAAATCACCACCAAAGCCTTTGGAACAGGAAGGC
GTATGCCAATTGTTCACAACTTTAAACTGTGA

Upstream 100 bases:

>100_bases
TTGAAAAAAAGCTGATAAGGTGGAGATAGAAAACAGTGTTTTTTGTCTTTTTATGTGCTAAAATATGTATTGTAGTACTA
TAAATGCGGGGTGAAAACTA

Downstream 100 bases:

>100_bases
TTTTTAAATAACTTGTGAGGAGGAGATATTTTGTCAAAACAATTAAAAAGCGACATTGTGCTTATCTTAGTAACTATGGT
GTGGGGGTCTACATTTATAA

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MKIALAQINPVVGDIKGNFEKIVEFINTAKYQKADLVVFPELATVGYPPKDFLFMQDFLEANEKYINEIVLPNTNQIGVI
LGTVRQDTEGNLYNSAFFIYEGEIVEIFDKTLLPNYDVFDEKRYFKPAQLRKVASFKGIKLGVNICEDIWKDYVFEPNVD
YSVDVLEEQFKLKPDIFINISASPYYLGKENMRVEMIEKKIKKYAIPFVYVNQVGANDELIFDGASFVVNEEGKRVVQLK
AFEEDIKVFDIDELKNLRKLPEIKEDISWVYNALVLGVRDYCKKSGFKKAVVGLSGGIDSAVVCAIAVEALGKENVLAVS
MPSRYSSEGSKSDARILADNLGIEFRVIPIEPVFKSYLSVFNGDNNVIGDLAEENIQARIRGNYLMFISNREGHIVLTTG
NKSELAMGYCTLYGDMSGSLAPIADVPKTMVYDLARFINREREIIPISIIEKAPSAELRPNQKDEDSLPPYKILDEILKM
YIEENRSAKEIIEKGYDERLVKDVINKVNRVEYKRKQAAPGLKITTKAFGTGRRMPIVHNFKL

Sequences:

>Translated_543_residues
MKIALAQINPVVGDIKGNFEKIVEFINTAKYQKADLVVFPELATVGYPPKDFLFMQDFLEANEKYINEIVLPNTNQIGVI
LGTVRQDTEGNLYNSAFFIYEGEIVEIFDKTLLPNYDVFDEKRYFKPAQLRKVASFKGIKLGVNICEDIWKDYVFEPNVD
YSVDVLEEQFKLKPDIFINISASPYYLGKENMRVEMIEKKIKKYAIPFVYVNQVGANDELIFDGASFVVNEEGKRVVQLK
AFEEDIKVFDIDELKNLRKLPEIKEDISWVYNALVLGVRDYCKKSGFKKAVVGLSGGIDSAVVCAIAVEALGKENVLAVS
MPSRYSSEGSKSDARILADNLGIEFRVIPIEPVFKSYLSVFNGDNNVIGDLAEENIQARIRGNYLMFISNREGHIVLTTG
NKSELAMGYCTLYGDMSGSLAPIADVPKTMVYDLARFINREREIIPISIIEKAPSAELRPNQKDEDSLPPYKILDEILKM
YIEENRSAKEIIEKGYDERLVKDVINKVNRVEYKRKQAAPGLKITTKAFGTGRRMPIVHNFKL
>Mature_543_residues
MKIALAQINPVVGDIKGNFEKIVEFINTAKYQKADLVVFPELATVGYPPKDFLFMQDFLEANEKYINEIVLPNTNQIGVI
LGTVRQDTEGNLYNSAFFIYEGEIVEIFDKTLLPNYDVFDEKRYFKPAQLRKVASFKGIKLGVNICEDIWKDYVFEPNVD
YSVDVLEEQFKLKPDIFINISASPYYLGKENMRVEMIEKKIKKYAIPFVYVNQVGANDELIFDGASFVVNEEGKRVVQLK
AFEEDIKVFDIDELKNLRKLPEIKEDISWVYNALVLGVRDYCKKSGFKKAVVGLSGGIDSAVVCAIAVEALGKENVLAVS
MPSRYSSEGSKSDARILADNLGIEFRVIPIEPVFKSYLSVFNGDNNVIGDLAEENIQARIRGNYLMFISNREGHIVLTTG
NKSELAMGYCTLYGDMSGSLAPIADVPKTMVYDLARFINREREIIPISIIEKAPSAELRPNQKDEDSLPPYKILDEILKM
YIEENRSAKEIIEKGYDERLVKDVINKVNRVEYKRKQAAPGLKITTKAFGTGRRMPIVHNFKL

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321865, Length=608, Percent_Identity=24.6710526315789, Blast_Score=100, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61553; Mature: 61553

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALAQINPVVGDIKGNFEKIVEFINTAKYQKADLVVFPELATVGYPPKDFLFMQDFLE
CEEEEEECCCHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHH
ANEKYINEIVLPNTNQIGVILGTVRQDTEGNLYNSAFFIYEGEIVEIFDKTLLPNYDVFD
HHHHHHHEEECCCCCCEEEEEEEEEECCCCCEEEEEEEEEECCHHHHHHHHCCCCCCCCC
EKRYFKPAQLRKVASFKGIKLGVNICEDIWKDYVFEPNVDYSVDVLEEQFKLKPDIFINI
CHHCCCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEE
SASPYYLGKENMRVEMIEKKIKKYAIPFVYVNQVGANDELIFDGASFVVNEEGKRVVQLK
CCCCEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCEEEEECCCCEEEEEE
AFEEDIKVFDIDELKNLRKLPEIKEDISWVYNALVLGVRDYCKKSGFKKAVVGLSGGIDS
EECCCCCEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
AVVCAIAVEALGKENVLAVSMPSRYSSEGSKSDARILADNLGIEFRVIPIEPVFKSYLSV
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHEEECCCCEEEEEEECCHHHHHHHHH
FNGDNNVIGDLAEENIQARIRGNYLMFISNREGHIVLTTGNKSELAMGYCTLYGDMSGSL
HCCCCCEECCHHHCCCEEEEECCEEEEEECCCCEEEEEECCCCCEEEEEEEEEECCCCCC
APIADVPKTMVYDLARFINREREIIPISIIEKAPSAELRPNQKDEDSLPPYKILDEILKM
CCHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YIEENRSAKEIIEKGYDERLVKDVINKVNRVEYKRKQAAPGLKITTKAFGTGRRMPIVHN
HHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEC
FKL
CCC
>Mature Secondary Structure
MKIALAQINPVVGDIKGNFEKIVEFINTAKYQKADLVVFPELATVGYPPKDFLFMQDFLE
CEEEEEECCCHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHH
ANEKYINEIVLPNTNQIGVILGTVRQDTEGNLYNSAFFIYEGEIVEIFDKTLLPNYDVFD
HHHHHHHEEECCCCCCEEEEEEEEEECCCCCEEEEEEEEEECCHHHHHHHHCCCCCCCCC
EKRYFKPAQLRKVASFKGIKLGVNICEDIWKDYVFEPNVDYSVDVLEEQFKLKPDIFINI
CHHCCCHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEE
SASPYYLGKENMRVEMIEKKIKKYAIPFVYVNQVGANDELIFDGASFVVNEEGKRVVQLK
CCCCEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCEEEEECCCCEEEEEE
AFEEDIKVFDIDELKNLRKLPEIKEDISWVYNALVLGVRDYCKKSGFKKAVVGLSGGIDS
EECCCCCEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
AVVCAIAVEALGKENVLAVSMPSRYSSEGSKSDARILADNLGIEFRVIPIEPVFKSYLSV
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHEEECCCCEEEEEEECCHHHHHHHHH
FNGDNNVIGDLAEENIQARIRGNYLMFISNREGHIVLTTGNKSELAMGYCTLYGDMSGSL
HCCCCCEECCHHHCCCEEEEECCEEEEEECCCCEEEEEECCCCCEEEEEEEEEECCCCCC
APIADVPKTMVYDLARFINREREIIPISIIEKAPSAELRPNQKDEDSLPPYKILDEILKM
CCHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YIEENRSAKEIIEKGYDERLVKDVINKVNRVEYKRKQAAPGLKITTKAFGTGRRMPIVHN
HHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEC
FKL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]