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The map label for this gene is folC [H]

Identifier: 167040832

GI number: 167040832

Start: 2210690

End: 2211979

Strand: Reverse

Name: folC [H]

Synonym: Teth514_2209

Alternate gene names: 167040832

Gene position: 2211979-2210690 (Counterclockwise)

Preceding gene: 167040833

Following gene: 167040831

Centisome position: 90.02

GC content: 34.26

Gene sequence:

>1290_bases
ATGAATTATGAAGAGGCGATTGGTTACATACACGGCATATATAAATTTGGTATAAAATTGGGGTTGGAAAATATAAGAAA
ATTATTGGACTACATGGGGAATCCTCAGAAAAGTCTTAAAATTATTCATGTTGCTGGTACAAATGGAAAAGGCTCTACAT
CAGCTTTTATAAGCAACATTTTGCAGCAGGCTGGATATAAAGTAGCTTTATATACTTCTCCCTACCTCGAAGAATTTGAA
GAGAGAATGAGAATAAATGGAGAAAATATTTCCAAGGAAAAGTTGATTTATTATGTTGAATATATAAAGCCTATAATATC
TCGTATGGTGGAGGAAGGATACAATCATCCGACGGAATTTGAAGTGGTAACTGCAATTGCTTTTAAATATTTTTACGATG
AAAAGGTGGATTTTGTAGTATTGGAAGTGGGGCTTGGAGGCAGATTTGACGCGACAAATGCCATAGATTCTTCTTTGGTT
TCTGTCATAACGACTATTGATTATGACCATACAGACAGATTGGGACATACTTTAGGAGAGATTGCATATGAAAAAGCCGG
AATCATCAAGCAAAAGGGGGTTGTAGTGAGTTTTTATCAACAACCAGAAGCGATGAAGGTCATCATAGAAGCCTGTGAGG
TAAGAAATGCTTATTTGACTGTACTTGAAAAAAGTAACGTAATTATAAAAGAACAAAACCCTGATTTTCAGATTTTTGAC
TACAAAAAATACAAGGACTTAAAAATTACTTTATTGGGAGAGCATCAGATTTACAATGCTGCTTTGGCGATTGAGAGCGT
AGAAAAATTAAAAATGTATGGATACGAAATCACAGAAAAAGATATAAGGGAAGGGCTTTACAGGGCGAAATGGCCTGGAA
GATTGGAAGTAATGAGAAAAAAACCCTACGTAGTAATAGACGGTGCTCACAATCCTCAAGGAATGACTGTTTTGAAAGAG
TCTCTAAAATTATTTAAATATGATAAATTGATTTTAGTAATTGGAATGCTTAAAGACAAAGATACACAGAAGATGTTGGA
TATAATTGTGCCAGAGGCAAATATGATAATAACAACCACCCCAATAAGTGAAAGAGCTTATAAAGCAAGTGAACTTGCAG
AGCGGATAGAAAAAGAAAAAGTGATTGCGATTGAAAATATTGAAGAAGCGGTAAAATACGCCCTGGATATAGCTAAGGAA
GAGGATATGGTACTTTTTTGTGGTTCATTGTACATGATTGGTTATGTGAGGTCTATGCTTAAAAAGGTAATAATTAAGAC
ATATTTTTAA

Upstream 100 bases:

>100_bases
GGAAGAAGAAGACGACAGTTTAAGATTGGAGATGAGTATGTAGACGAAGTTTTAATGGGGTTGTTTTTATAAAATTAGTC
TTTATAGGGGAGGGATTTAA

Downstream 100 bases:

>100_bases
TATAATGTAAGGTGTAAAGGCCGTAATGAGAAGGTGGTTGTTATGGAAATGACAGGGACTGCGTTGTTACGGGAGATAGA
TAAAGCACTAAAGGCGATGA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNYEEAIGYIHGIYKFGIKLGLENIRKLLDYMGNPQKSLKIIHVAGTNGKGSTSAFISNILQQAGYKVALYTSPYLEEFE
ERMRINGENISKEKLIYYVEYIKPIISRMVEEGYNHPTEFEVVTAIAFKYFYDEKVDFVVLEVGLGGRFDATNAIDSSLV
SVITTIDYDHTDRLGHTLGEIAYEKAGIIKQKGVVVSFYQQPEAMKVIIEACEVRNAYLTVLEKSNVIIKEQNPDFQIFD
YKKYKDLKITLLGEHQIYNAALAIESVEKLKMYGYEITEKDIREGLYRAKWPGRLEVMRKKPYVVIDGAHNPQGMTVLKE
SLKLFKYDKLILVIGMLKDKDTQKMLDIIVPEANMIITTTPISERAYKASELAERIEKEKVIAIENIEEAVKYALDIAKE
EDMVLFCGSLYMIGYVRSMLKKVIIKTYF

Sequences:

>Translated_429_residues
MNYEEAIGYIHGIYKFGIKLGLENIRKLLDYMGNPQKSLKIIHVAGTNGKGSTSAFISNILQQAGYKVALYTSPYLEEFE
ERMRINGENISKEKLIYYVEYIKPIISRMVEEGYNHPTEFEVVTAIAFKYFYDEKVDFVVLEVGLGGRFDATNAIDSSLV
SVITTIDYDHTDRLGHTLGEIAYEKAGIIKQKGVVVSFYQQPEAMKVIIEACEVRNAYLTVLEKSNVIIKEQNPDFQIFD
YKKYKDLKITLLGEHQIYNAALAIESVEKLKMYGYEITEKDIREGLYRAKWPGRLEVMRKKPYVVIDGAHNPQGMTVLKE
SLKLFKYDKLILVIGMLKDKDTQKMLDIIVPEANMIITTTPISERAYKASELAERIEKEKVIAIENIEEAVKYALDIAKE
EDMVLFCGSLYMIGYVRSMLKKVIIKTYF
>Mature_429_residues
MNYEEAIGYIHGIYKFGIKLGLENIRKLLDYMGNPQKSLKIIHVAGTNGKGSTSAFISNILQQAGYKVALYTSPYLEEFE
ERMRINGENISKEKLIYYVEYIKPIISRMVEEGYNHPTEFEVVTAIAFKYFYDEKVDFVVLEVGLGGRFDATNAIDSSLV
SVITTIDYDHTDRLGHTLGEIAYEKAGIIKQKGVVVSFYQQPEAMKVIIEACEVRNAYLTVLEKSNVIIKEQNPDFQIFD
YKKYKDLKITLLGEHQIYNAALAIESVEKLKMYGYEITEKDIREGLYRAKWPGRLEVMRKKPYVVIDGAHNPQGMTVLKE
SLKLFKYDKLILVIGMLKDKDTQKMLDIIVPEANMIITTTPISERAYKASELAERIEKEKVIAIENIEEAVKYALDIAKE
EDMVLFCGSLYMIGYVRSMLKKVIIKTYF

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=360, Percent_Identity=30.5555555555556, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI66932984, Length=360, Percent_Identity=30.5555555555556, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI1788654, Length=419, Percent_Identity=31.980906921241, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17553148, Length=493, Percent_Identity=26.7748478701826, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17553150, Length=493, Percent_Identity=26.7748478701826, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI71984923, Length=493, Percent_Identity=26.7748478701826, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323760, Length=426, Percent_Identity=32.6291079812207, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6324815, Length=467, Percent_Identity=28.6937901498929, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6322718, Length=296, Percent_Identity=28.7162162162162, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24641571, Length=306, Percent_Identity=32.3529411764706, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24581568, Length=174, Percent_Identity=32.183908045977, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 49188; Mature: 49188

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYEEAIGYIHGIYKFGIKLGLENIRKLLDYMGNPQKSLKIIHVAGTNGKGSTSAFISNI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHH
LQQAGYKVALYTSPYLEEFEERMRINGENISKEKLIYYVEYIKPIISRMVEEGYNHPTEF
HHHCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
EVVTAIAFKYFYDEKVDFVVLEVGLGGRFDATNAIDSSLVSVITTIDYDHTDRLGHTLGE
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IAYEKAGIIKQKGVVVSFYQQPEAMKVIIEACEVRNAYLTVLEKSNVIIKEQNPDFQIFD
HHHHHCCCEEECCEEEEEECCCHHHHHHHHHHHCCCHHEEEEECCCEEEEECCCCEEEEE
YKKYKDLKITLLGEHQIYNAALAIESVEKLKMYGYEITEKDIREGLYRAKWPGRLEVMRK
ECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHCCCCCHHHHEEC
KPYVVIDGAHNPQGMTVLKESLKLFKYDKLILVIGMLKDKDTQKMLDIIVPEANMIITTT
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEC
PISERAYKASELAERIEKEKVIAIENIEEAVKYALDIAKEEDMVLFCGSLYMIGYVRSML
CCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHH
KKVIIKTYF
HHHHHHCCC
>Mature Secondary Structure
MNYEEAIGYIHGIYKFGIKLGLENIRKLLDYMGNPQKSLKIIHVAGTNGKGSTSAFISNI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHH
LQQAGYKVALYTSPYLEEFEERMRINGENISKEKLIYYVEYIKPIISRMVEEGYNHPTEF
HHHCCCEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
EVVTAIAFKYFYDEKVDFVVLEVGLGGRFDATNAIDSSLVSVITTIDYDHTDRLGHTLGE
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IAYEKAGIIKQKGVVVSFYQQPEAMKVIIEACEVRNAYLTVLEKSNVIIKEQNPDFQIFD
HHHHHCCCEEECCEEEEEECCCHHHHHHHHHHHCCCHHEEEEECCCEEEEECCCCEEEEE
YKKYKDLKITLLGEHQIYNAALAIESVEKLKMYGYEITEKDIREGLYRAKWPGRLEVMRK
ECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHCCCCCHHHHEEC
KPYVVIDGAHNPQGMTVLKESLKLFKYDKLILVIGMLKDKDTQKMLDIIVPEANMIITTT
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEC
PISERAYKASELAERIEKEKVIAIENIEEAVKYALDIAKEEDMVLFCGSLYMIGYVRSML
CCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHH
KKVIIKTYF
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]