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The map label for this gene is mscL [H]

Identifier: 167040474

GI number: 167040474

Start: 1861324

End: 1861704

Strand: Reverse

Name: mscL [H]

Synonym: Teth514_1843

Alternate gene names: 167040474

Gene position: 1861704-1861324 (Counterclockwise)

Preceding gene: 167040475

Following gene: 167040471

Centisome position: 75.76

GC content: 29.92

Gene sequence:

>381_bases
ATGTTAAAAGGATTTAAAGAATTTATTATGAGAGGAAATGTTTTAGATTTAGCTGTAGCTGTAATAATAGGTGGAGCATT
TAATAAGATTGTCAATTCATTAGTAGTTGATGTATTAACTCCATTAATCGGAGCAATATTTGGAGCACCAGACTTTTCTG
CTATTAAATTCGGACCTATATCAATAGGCAACTTCATAAATGCAGTTATTAACTTTATAGTTATAGCTGCCGCAATATAT
TTTTTTGTAGTAGCTCCTATTAATACAATCAAAAATCGCAAAGAAAAACAAAAACAACAACAAACACCACAAGCATCTCC
TGAGCCTTCAGAGGAAGTAAAACTTTTAAGGGAAATTTTAGATATGTTAAAAGAAAAGTAA

Upstream 100 bases:

>100_bases
CCGGTTCATCCATCGGCGGGGGAGGAGGATTTGGAGGTAGCGGAGGAGGAGCGAGGTAGAATAAAAATGTACAATAATTT
ATTTTAGAGAGGAGAAAATT

Downstream 100 bases:

>100_bases
TGTAAGAATCAGTTAACATTTTTATCTGTTCTATCACTAGTATAATCTTTTTCTATATCTAATGAATGAAAGTGAATAAA
TTTGGTTAAAATATTTAATT

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MLKGFKEFIMRGNVLDLAVAVIIGGAFNKIVNSLVVDVLTPLIGAIFGAPDFSAIKFGPISIGNFINAVINFIVIAAAIY
FFVVAPINTIKNRKEKQKQQQTPQASPEPSEEVKLLREILDMLKEK

Sequences:

>Translated_126_residues
MLKGFKEFIMRGNVLDLAVAVIIGGAFNKIVNSLVVDVLTPLIGAIFGAPDFSAIKFGPISIGNFINAVINFIVIAAAIY
FFVVAPINTIKNRKEKQKQQQTPQASPEPSEEVKLLREILDMLKEK
>Mature_126_residues
MLKGFKEFIMRGNVLDLAVAVIIGGAFNKIVNSLVVDVLTPLIGAIFGAPDFSAIKFGPISIGNFINAVINFIVIAAAIY
FFVVAPINTIKNRKEKQKQQQTPQASPEPSEEVKLLREILDMLKEK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=137, Percent_Identity=43.0656934306569, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 13816; Mature: 13816

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGFKEFIMRGNVLDLAVAVIIGGAFNKIVNSLVVDVLTPLIGAIFGAPDFSAIKFGPI
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCC
SIGNFINAVINFIVIAAAIYFFVVAPINTIKNRKEKQKQQQTPQASPEPSEEVKLLREIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DMLKEK
HHHHCC
>Mature Secondary Structure
MLKGFKEFIMRGNVLDLAVAVIIGGAFNKIVNSLVVDVLTPLIGAIFGAPDFSAIKFGPI
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCC
SIGNFINAVINFIVIAAAIYFFVVAPINTIKNRKEKQKQQQTPQASPEPSEEVKLLREIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DMLKEK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA