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The map label for this gene is mcrB [H]

Identifier: 167040459

GI number: 167040459

Start: 1836952

End: 1838703

Strand: Reverse

Name: mcrB [H]

Synonym: Teth514_1826

Alternate gene names: 167040459

Gene position: 1838703-1836952 (Counterclockwise)

Preceding gene: 167040460

Following gene: 167040458

Centisome position: 74.83

GC content: 29.51

Gene sequence:

>1752_bases
ATGTCACTAAAGACTCTTTTTAAAGAGGTTATGGATAATTATATAAAAGCAAGAACCTCACAGCCATTTAAAGGAAATAG
AATAGGAGATATATTACGAAAAGAAATACCTCAGGAGATACAAAAATTTGATTTTATAGGTGATGAATATTTTATTAAAG
GCTCTAATGGTCAAGGTAACTGGGCAGAAATTCCTTGGGTTGCTATTATGGACAAAAATATTACACAAACTACAATGGAA
GGGGTATATATTGTTTATCTATTTTCTTCTGATATGAAAAGGCTATATTTAACTTTGAATCAAGGCTATACTAAGTTAAA
AGATAAATATGGTAAACTTGTGGCCATTAAAAAAATGTTATCTCTTGCTTTAAAGATAAGGAGTAAAATGGAGGCAAAGG
GATGGAATACAGATAATAATTTATCAATTGGCAATGAATTTTATGAAAAAGGGACAATATTTTATAAAAAATATGAGAAT
AATGATTTACCAGATGATGAAACATTAAAAAATGACCTTCATGATTTGATAAATATTTATAAAAATGTTTCTGTTTTAAG
CGGTGAAGATAAGGAACAAATATATGAAAATGAGGAAGATTATATACCTGACAACGATACTAGTTATAACGTCAAAGATG
AACTTGATTACATAAAAAGTTACATAAAAAATCATGGGTTTAGCTACAATGATAAGTTAATAGAAAATTTTTATTTATCC
TTGAAGTCAAAGCCATTTTTAATTCTTGCTGGTATTTCAGGTACTGGGAAAAGCAAACTTGCAAGACTTTTTGCGGAGGC
TATCGGTTGCAATACCAAGAATGGACGATTTATGTTGGTACCTGTAAGACCCGACTGGTCAGATTCTACGGAATTATTAG
GTTACAAAGATATGCATAATAAATTTCATCCTGGTGTTTTAACAAATTTTATAAAAAAGGCTATAAACGATATAAATAGA
CCTTACTTTTTTGTATTGGATGAAATGAACCTTGCACGTGTAGAGTATTATTTCAGCGATATTCTTTCAATTATAGAGTC
ACGAAAAAAGGATGGCGATAGAATTGTTACAGATCCCCTTTTGATCAAAGAACTGTTAGATGAAAATTCTTTCCATGAAT
ATGGCAATTTGTACATACCTGAAAATTTATATTTCATTGGCACTGTTAATATGGATGAGACTACGTTTCCCTTTAGCAAA
AAAGTGCTTGATAGAGCAAATGTTATTGAGTTTTCTGATGTAAACCTCGATTATTTTGTTGGAGATATCGAAGAAATTAC
TGAAAAAGTATTAAACAATAGTTTTTTGAAGAGTGAATTTTTAACATTAAATGATTGTCTTGATTATAGGGAAATTATTG
ATGATGTGATTTTGGTTTTAAAAAAAATTAATGATGTTCTAAGAGAAGGAAATTTGCATTTTGGTTATAGGGTGAGAGAT
GAAATAAGTTTTTATATGATATACAATGAGTTAAATGGTTTAATGGATTTTGATGAAGCGATGGATTTAGAAATATTGCA
AAAGATTTTGCCCAGGATACACGGAAGCAGTATCAGTATAAAGAAAATACTTGTCGAATTATTTAAAATATGTTCTGGCA
ATTATGAAGCCAAGTATGATTATGAGGATATGGATGTTAGTGATAAGATGCTTAAAGATATGGATAATTGTGTATATCCA
AGGTCAGCAGAAAAAATTATTTATATGGTAAGGAGATATGAAGAAGATGGATTTACGTCATACTGGCTCTGA

Upstream 100 bases:

>100_bases
AAATACATGTCGAAATATATAAAGTGTTTAATTAAATAAAAATGGGATATTTTGAATAATAACTAGAGAAATTTATATTT
TTGAGAAAGGTAAGATAAAA

Downstream 100 bases:

>100_bases
TGTTGACCTGCTTTATATATCTACTCCGAAATTTGATTTTTCAATAAAAGGCAATCCCTATCATCCTAATGTTGGAGCTT
TAAATATCAATGAAAACGTA

Product: ATPase

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MSLKTLFKEVMDNYIKARTSQPFKGNRIGDILRKEIPQEIQKFDFIGDEYFIKGSNGQGNWAEIPWVAIMDKNITQTTME
GVYIVYLFSSDMKRLYLTLNQGYTKLKDKYGKLVAIKKMLSLALKIRSKMEAKGWNTDNNLSIGNEFYEKGTIFYKKYEN
NDLPDDETLKNDLHDLINIYKNVSVLSGEDKEQIYENEEDYIPDNDTSYNVKDELDYIKSYIKNHGFSYNDKLIENFYLS
LKSKPFLILAGISGTGKSKLARLFAEAIGCNTKNGRFMLVPVRPDWSDSTELLGYKDMHNKFHPGVLTNFIKKAINDINR
PYFFVLDEMNLARVEYYFSDILSIIESRKKDGDRIVTDPLLIKELLDENSFHEYGNLYIPENLYFIGTVNMDETTFPFSK
KVLDRANVIEFSDVNLDYFVGDIEEITEKVLNNSFLKSEFLTLNDCLDYREIIDDVILVLKKINDVLREGNLHFGYRVRD
EISFYMIYNELNGLMDFDEAMDLEILQKILPRIHGSSISIKKILVELFKICSGNYEAKYDYEDMDVSDKMLKDMDNCVYP
RSAEKIIYMVRRYEEDGFTSYWL

Sequences:

>Translated_583_residues
MSLKTLFKEVMDNYIKARTSQPFKGNRIGDILRKEIPQEIQKFDFIGDEYFIKGSNGQGNWAEIPWVAIMDKNITQTTME
GVYIVYLFSSDMKRLYLTLNQGYTKLKDKYGKLVAIKKMLSLALKIRSKMEAKGWNTDNNLSIGNEFYEKGTIFYKKYEN
NDLPDDETLKNDLHDLINIYKNVSVLSGEDKEQIYENEEDYIPDNDTSYNVKDELDYIKSYIKNHGFSYNDKLIENFYLS
LKSKPFLILAGISGTGKSKLARLFAEAIGCNTKNGRFMLVPVRPDWSDSTELLGYKDMHNKFHPGVLTNFIKKAINDINR
PYFFVLDEMNLARVEYYFSDILSIIESRKKDGDRIVTDPLLIKELLDENSFHEYGNLYIPENLYFIGTVNMDETTFPFSK
KVLDRANVIEFSDVNLDYFVGDIEEITEKVLNNSFLKSEFLTLNDCLDYREIIDDVILVLKKINDVLREGNLHFGYRVRD
EISFYMIYNELNGLMDFDEAMDLEILQKILPRIHGSSISIKKILVELFKICSGNYEAKYDYEDMDVSDKMLKDMDNCVYP
RSAEKIIYMVRRYEEDGFTSYWL
>Mature_582_residues
SLKTLFKEVMDNYIKARTSQPFKGNRIGDILRKEIPQEIQKFDFIGDEYFIKGSNGQGNWAEIPWVAIMDKNITQTTMEG
VYIVYLFSSDMKRLYLTLNQGYTKLKDKYGKLVAIKKMLSLALKIRSKMEAKGWNTDNNLSIGNEFYEKGTIFYKKYENN
DLPDDETLKNDLHDLINIYKNVSVLSGEDKEQIYENEEDYIPDNDTSYNVKDELDYIKSYIKNHGFSYNDKLIENFYLSL
KSKPFLILAGISGTGKSKLARLFAEAIGCNTKNGRFMLVPVRPDWSDSTELLGYKDMHNKFHPGVLTNFIKKAINDINRP
YFFVLDEMNLARVEYYFSDILSIIESRKKDGDRIVTDPLLIKELLDENSFHEYGNLYIPENLYFIGTVNMDETTFPFSKK
VLDRANVIEFSDVNLDYFVGDIEEITEKVLNNSFLKSEFLTLNDCLDYREIIDDVILVLKKINDVLREGNLHFGYRVRDE
ISFYMIYNELNGLMDFDEAMDLEILQKILPRIHGSSISIKKILVELFKICSGNYEAKYDYEDMDVSDKMLKDMDNCVYPR
SAEKIIYMVRRYEEDGFTSYWL

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=372, Percent_Identity=23.6559139784946, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 68181; Mature: 68049

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKTLFKEVMDNYIKARTSQPFKGNRIGDILRKEIPQEIQKFDFIGDEYFIKGSNGQGN
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
WAEIPWVAIMDKNITQTTMEGVYIVYLFSSDMKRLYLTLNQGYTKLKDKYGKLVAIKKML
CEECCEEEEECCCCHHHHHCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
SLALKIRSKMEAKGWNTDNNLSIGNEFYEKGTIFYKKYENNDLPDDETLKNDLHDLINIY
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
KNVSVLSGEDKEQIYENEEDYIPDNDTSYNVKDELDYIKSYIKNHGFSYNDKLIENFYLS
HCCHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LKSKPFLILAGISGTGKSKLARLFAEAIGCNTKNGRFMLVPVRPDWSDSTELLGYKDMHN
CCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHCCCHHHCC
KFHPGVLTNFIKKAINDINRPYFFVLDEMNLARVEYYFSDILSIIESRKKDGDRIVTDPL
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHH
LIKELLDENSFHEYGNLYIPENLYFIGTVNMDETTFPFSKKVLDRANVIEFSDVNLDYFV
HHHHHHCCCCCHHCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHCCEEEEECCCHHHHH
GDIEEITEKVLNNSFLKSEFLTLNDCLDYREIIDDVILVLKKINDVLREGNLHFGYRVRD
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
EISFYMIYNELNGLMDFDEAMDLEILQKILPRIHGSSISIKKILVELFKICSGNYEAKYD
CEEEEEEEHHHCCCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECC
YEDMDVSDKMLKDMDNCVYPRSAEKIIYMVRRYEEDGFTSYWL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure 
SLKTLFKEVMDNYIKARTSQPFKGNRIGDILRKEIPQEIQKFDFIGDEYFIKGSNGQGN
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
WAEIPWVAIMDKNITQTTMEGVYIVYLFSSDMKRLYLTLNQGYTKLKDKYGKLVAIKKML
CEECCEEEEECCCCHHHHHCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
SLALKIRSKMEAKGWNTDNNLSIGNEFYEKGTIFYKKYENNDLPDDETLKNDLHDLINIY
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
KNVSVLSGEDKEQIYENEEDYIPDNDTSYNVKDELDYIKSYIKNHGFSYNDKLIENFYLS
HCCHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LKSKPFLILAGISGTGKSKLARLFAEAIGCNTKNGRFMLVPVRPDWSDSTELLGYKDMHN
CCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHCCCHHHCC
KFHPGVLTNFIKKAINDINRPYFFVLDEMNLARVEYYFSDILSIIESRKKDGDRIVTDPL
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHH
LIKELLDENSFHEYGNLYIPENLYFIGTVNMDETTFPFSKKVLDRANVIEFSDVNLDYFV
HHHHHHCCCCCHHCCCEEECCCEEEEEEECCCCCCCCHHHHHHHHCCEEEEECCCHHHHH
GDIEEITEKVLNNSFLKSEFLTLNDCLDYREIIDDVILVLKKINDVLREGNLHFGYRVRD
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
EISFYMIYNELNGLMDFDEAMDLEILQKILPRIHGSSISIKKILVELFKICSGNYEAKYD
CEEEEEEEHHHCCCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECC
YEDMDVSDKMLKDMDNCVYPRSAEKIIYMVRRYEEDGFTSYWL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]