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The map label for this gene is trmD [H]

Identifier: 167040346

GI number: 167040346

Start: 1718781

End: 1719509

Strand: Reverse

Name: trmD [H]

Synonym: Teth514_1709

Alternate gene names: 167040346

Gene position: 1719509-1718781 (Counterclockwise)

Preceding gene: 167040347

Following gene: 167040345

Centisome position: 69.98

GC content: 34.02

Gene sequence:

>729_bases
ATGATGATTTTTGACGTTTTAACCCTATTTCCTGAAATGTTTGATAGTGTTTTATCCTATAGTATATTGAAAAGGGCTAT
TGAAAAAGGAAAAATAAAAGTTAATTTGATAAATATAAGAGATTTTTCAAAGGATAAACACAAAAGAGTAGATGATTATC
CTTATGGAGGCGGGCCAGGAATGGTGATGATGCCTCAGCCTCTCTTTGATGCAATTGAATTTGTAAAAAGTCAAGGAAAT
ATCCCTGTATATTACCTTGGTCCTAAAGGTAAAATTTTTAATCAGGAAATGGCGGTTAAGATGTCTAAATTAGAAAGAAT
AGCCCTTTTGTGTGGCCACTATGAAGGAATAGATGAAAGAGTCTATTCTGTCATTGATGAAGAAATTTCTTTAGGAGATT
TTATATTGACAGGCGGAGAGATAGCCGCCATGGCTATCATTGACGCTGTAACAAGACTTATTGAAGGAGTACTTTCACAC
TCTCAAAGTGCTATAGAAGAATCCTTTGCTAATAATTTATTGGAATATCCACAGTATACAAGGCCAGAAGTTTTTAGAGG
TATGAAGGTGCCAGAAATTTTACTAAGTGGTAATCATGCCGAAATAGCTAAATGGCGAAGGCAAAAGTCTTTAGAGATAA
CTCTTTTAAAACGGCCTGATTTGCTTAAAAAGGCTGTTTTGACTGAAGAAGATAAAAAGTTTTTAAGAGAAAAAGGGTTT
GACATTTAG

Upstream 100 bases:

>100_bases
AAACTGAGGAGAGGGAAATACTAATTCCTGCTATAAAAGAAGTGGTTAAAAAAGTTGATGTTGACAATAAAGTAATGGTG
GTCCACCTTCTGGAGGGGCT

Downstream 100 bases:

>100_bases
AAGGTGGAAGGAGGGAAAAATAATGGACTTAATAAAAGCAGTTGAAAGTCAGCAGATGAGAAAAGACTTAACTCCTTTTA
ATGTAGGAGATACTATAAGG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MMIFDVLTLFPEMFDSVLSYSILKRAIEKGKIKVNLINIRDFSKDKHKRVDDYPYGGGPGMVMMPQPLFDAIEFVKSQGN
IPVYYLGPKGKIFNQEMAVKMSKLERIALLCGHYEGIDERVYSVIDEEISLGDFILTGGEIAAMAIIDAVTRLIEGVLSH
SQSAIEESFANNLLEYPQYTRPEVFRGMKVPEILLSGNHAEIAKWRRQKSLEITLLKRPDLLKKAVLTEEDKKFLREKGF
DI

Sequences:

>Translated_242_residues
MMIFDVLTLFPEMFDSVLSYSILKRAIEKGKIKVNLINIRDFSKDKHKRVDDYPYGGGPGMVMMPQPLFDAIEFVKSQGN
IPVYYLGPKGKIFNQEMAVKMSKLERIALLCGHYEGIDERVYSVIDEEISLGDFILTGGEIAAMAIIDAVTRLIEGVLSH
SQSAIEESFANNLLEYPQYTRPEVFRGMKVPEILLSGNHAEIAKWRRQKSLEITLLKRPDLLKKAVLTEEDKKFLREKGF
DI
>Mature_242_residues
MMIFDVLTLFPEMFDSVLSYSILKRAIEKGKIKVNLINIRDFSKDKHKRVDDYPYGGGPGMVMMPQPLFDAIEFVKSQGN
IPVYYLGPKGKIFNQEMAVKMSKLERIALLCGHYEGIDERVYSVIDEEISLGDFILTGGEIAAMAIIDAVTRLIEGVLSH
SQSAIEESFANNLLEYPQYTRPEVFRGMKVPEILLSGNHAEIAKWRRQKSLEITLLKRPDLLKKAVLTEEDKKFLREKGF
DI

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=239, Percent_Identity=49.7907949790795, Blast_Score=243, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27566; Mature: 27566

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIFDVLTLFPEMFDSVLSYSILKRAIEKGKIKVNLINIRDFSKDKHKRVDDYPYGGGPG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHCCCCCCCCCCCCC
MVMMPQPLFDAIEFVKSQGNIPVYYLGPKGKIFNQEMAVKMSKLERIALLCGHYEGIDER
EEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
VYSVIDEEISLGDFILTGGEIAAMAIIDAVTRLIEGVLSHSQSAIEESFANNLLEYPQYT
HHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RPEVFRGMKVPEILLSGNHAEIAKWRRQKSLEITLLKRPDLLKKAVLTEEDKKFLREKGF
CHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCHHHHHHHHHCCC
DI
CC
>Mature Secondary Structure
MMIFDVLTLFPEMFDSVLSYSILKRAIEKGKIKVNLINIRDFSKDKHKRVDDYPYGGGPG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHCCCCCCCCCCCCC
MVMMPQPLFDAIEFVKSQGNIPVYYLGPKGKIFNQEMAVKMSKLERIALLCGHYEGIDER
EEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
VYSVIDEEISLGDFILTGGEIAAMAIIDAVTRLIEGVLSHSQSAIEESFANNLLEYPQYT
HHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RPEVFRGMKVPEILLSGNHAEIAKWRRQKSLEITLLKRPDLLKKAVLTEEDKKFLREKGF
CHHHHCCCCCCHHHCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCHHHHHHHHHCCC
DI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]