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The map label for this gene is truB [H]

Identifier: 167040279

GI number: 167040279

Start: 1659396

End: 1660247

Strand: Reverse

Name: truB [H]

Synonym: Teth514_1642

Alternate gene names: 167040279

Gene position: 1660247-1659396 (Counterclockwise)

Preceding gene: 167040280

Following gene: 167040278

Centisome position: 67.56

GC content: 32.63

Gene sequence:

>852_bases
TTGGAGGGAATGCTCAATATTTTAAAACCCCCTGGTATGACTTCTCACGATGTTGTAGATTTTATTAGGAAAATCTATAA
TATACGAAAAGTGGGGCATACAGGAACTTTAGACCCAGATGCAGCAGGAATACTGCCTGTTTGTGTAGGGAATGCCACTA
AATTTGTCTCTTATCTTCTTGAGCACGACAAAAGATATAGATTTGAAATAACCTTTGGCTTTACTACAGACACTTTGGAT
AAAAGCGGCAAAATTGTAAAAAGCGGACCTGTAAGGATTCTGAGGGAAGAAGAATTAAGGCAAGTCTTAAATGAATTTAA
AGGGAAAATAAAACAAATTCCTCCAATGTATTCTGCAAAGAAAATAAAAGGAAAAAAGTTATACGAATATGCAAGAGAAG
GAAAGACAATTGATATTCCACCTATTGAGGTTACCATATATGATATTGAATTAGTTGAATATGAACCACCTTATCGGGTA
TTAATAGATGTGAAATGTTCTAAAGGCACCTATGTGAGAGCTTTAGTAAGAGATATATGCGAAAAATTAAACATGCCGGG
ATATATGTCTATGTTGATAAGAACAGAGGTTGGTGCTTTTAAAATAGAAGATGCTTATACTCTTGAAGAAATAAGGGAAA
AGAAAGTATTATTGCAGCCAATAGATAAGTTTATTGAATTACCTTCTGTACAATTAAACAAAATGGAAGCTGAAAAAATT
TTAAAAGGCCAATTTATTCAAAATAGATATCCTATTGAAACTTCACTTTTGAAGTTATATGATAATAATGGTAAATTTCT
CGGCATTGGGATTTTAGAGCGAAATAAAATCCGTCCAAAAAGACTTCTTTGA

Upstream 100 bases:

>100_bases
AGGCCATGTAAGAGCTGCTGGATGTATCATCAAAGGAAATATCCAAGAAGCCAAAGAAAAAATTTTACAGGTAATATTCA
AAGAATTAGAGGGATAAAAA

Downstream 100 bases:

>100_bases
GGGGGAAATGATAATTTGCAGATTGTTTATGAAAGCAATGCATCAAAATACAAAGAGGCCAAAGTAATAGCTTTAGGCAA
TTTTGATGGAGTTCATATAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MEGMLNILKPPGMTSHDVVDFIRKIYNIRKVGHTGTLDPDAAGILPVCVGNATKFVSYLLEHDKRYRFEITFGFTTDTLD
KSGKIVKSGPVRILREEELRQVLNEFKGKIKQIPPMYSAKKIKGKKLYEYAREGKTIDIPPIEVTIYDIELVEYEPPYRV
LIDVKCSKGTYVRALVRDICEKLNMPGYMSMLIRTEVGAFKIEDAYTLEEIREKKVLLQPIDKFIELPSVQLNKMEAEKI
LKGQFIQNRYPIETSLLKLYDNNGKFLGIGILERNKIRPKRLL

Sequences:

>Translated_283_residues
MEGMLNILKPPGMTSHDVVDFIRKIYNIRKVGHTGTLDPDAAGILPVCVGNATKFVSYLLEHDKRYRFEITFGFTTDTLD
KSGKIVKSGPVRILREEELRQVLNEFKGKIKQIPPMYSAKKIKGKKLYEYAREGKTIDIPPIEVTIYDIELVEYEPPYRV
LIDVKCSKGTYVRALVRDICEKLNMPGYMSMLIRTEVGAFKIEDAYTLEEIREKKVLLQPIDKFIELPSVQLNKMEAEKI
LKGQFIQNRYPIETSLLKLYDNNGKFLGIGILERNKIRPKRLL
>Mature_283_residues
MEGMLNILKPPGMTSHDVVDFIRKIYNIRKVGHTGTLDPDAAGILPVCVGNATKFVSYLLEHDKRYRFEITFGFTTDTLD
KSGKIVKSGPVRILREEELRQVLNEFKGKIKQIPPMYSAKKIKGKKLYEYAREGKTIDIPPIEVTIYDIELVEYEPPYRV
LIDVKCSKGTYVRALVRDICEKLNMPGYMSMLIRTEVGAFKIEDAYTLEEIREKKVLLQPIDKFIELPSVQLNKMEAEKI
LKGQFIQNRYPIETSLLKLYDNNGKFLGIGILERNKIRPKRLL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=236, Percent_Identity=32.2033898305085, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI215599015, Length=203, Percent_Identity=29.5566502463054, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4503337, Length=203, Percent_Identity=29.5566502463054, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI2367200, Length=307, Percent_Identity=37.4592833876222, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17553978, Length=218, Percent_Identity=26.605504587156, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324037, Length=133, Percent_Identity=42.8571428571429, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=216, Percent_Identity=28.2407407407407, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281364189, Length=197, Percent_Identity=28.4263959390863, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364187, Length=197, Percent_Identity=28.4263959390863, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364185, Length=197, Percent_Identity=28.4263959390863, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364183, Length=197, Percent_Identity=28.4263959390863, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62471759, Length=197, Percent_Identity=28.4263959390863, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17975520, Length=197, Percent_Identity=28.4263959390863, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32618; Mature: 32618

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGMLNILKPPGMTSHDVVDFIRKIYNIRKVGHTGTLDPDAAGILPVCVGNATKFVSYLL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECCHHHHHHHHH
EHDKRYRFEITFGFTTDTLDKSGKIVKSGPVRILREEELRQVLNEFKGKIKQIPPMYSAK
HCCCEEEEEEEEECEECCCCCCCCEEECCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHH
KIKGKKLYEYAREGKTIDIPPIEVTIYDIELVEYEPPYRVLIDVKCSKGTYVRALVRDIC
HCCHHHHHHHHHCCCEEECCCEEEEEEEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHH
EKLNMPGYMSMLIRTEVGAFKIEDAYTLEEIREKKVLLQPIDKFIELPSVQLNKMEAEKI
HHCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LKGQFIQNRYPIETSLLKLYDNNGKFLGIGILERNKIRPKRLL
HHHHHHCCCCCHHHEEEEEECCCCCEEEEEEEECCCCCHHCCC
>Mature Secondary Structure
MEGMLNILKPPGMTSHDVVDFIRKIYNIRKVGHTGTLDPDAAGILPVCVGNATKFVSYLL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECCHHHHHHHHH
EHDKRYRFEITFGFTTDTLDKSGKIVKSGPVRILREEELRQVLNEFKGKIKQIPPMYSAK
HCCCEEEEEEEEECEECCCCCCCCEEECCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHH
KIKGKKLYEYAREGKTIDIPPIEVTIYDIELVEYEPPYRVLIDVKCSKGTYVRALVRDIC
HCCHHHHHHHHHCCCEEECCCEEEEEEEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHH
EKLNMPGYMSMLIRTEVGAFKIEDAYTLEEIREKKVLLQPIDKFIELPSVQLNKMEAEKI
HHCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LKGQFIQNRYPIETSLLKLYDNNGKFLGIGILERNKIRPKRLL
HHHHHHCCCCCHHHEEEEEECCCCCEEEEEEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]