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The map label for this gene is miaB [H]

Identifier: 167040247

GI number: 167040247

Start: 1625318

End: 1626733

Strand: Direct

Name: miaB [H]

Synonym: Teth514_1610

Alternate gene names: 167040247

Gene position: 1625318-1626733 (Clockwise)

Preceding gene: 167040246

Following gene: 167040248

Centisome position: 66.14

GC content: 31.78

Gene sequence:

>1416_bases
ATGCCTACCAATATTTATGTGACTGAAGAAGAATTAAAAAAACAAGAAAAAATAATGAAAGAAATTGCAGAAGAAAATAA
GGGTAAAAACCTTTATTATCACATTGAAACTTACGGCTGCCAAATGAATGTCCATGATTCAGAAAAACTAGCAGGTATGT
TAGAAAAGATGGGATATAAATATACTGAAAATCTGGAACAAGCTGATGTTTTACTTTTTAACACTTGTGCTGTAAGAGAA
CATGCTGAAATAAGAGTATTGGGAAGAGTATCACAAATGAAAGAATTGAAGGCTAGAAATCCTAATTTAATTATTGGTGT
ATCTGGCTGTATGATGCAAGAAAAAAATGTAGTAGAAGCTATTAAAGAAAAATATTCCTATATAGACATTGTTTTTGGCA
CTCATAATATATATAAATTTCCACAGCTTTTATGGGAGGCTTTGAACTCTCAAGATATAGTTATAGATATTATAGAAGAT
ACAAAAAATGTAATTGAAGAGTTACCTGTCAAAAGGGACAGCAATTTAAAGGCATGGGTTAATATTATTTATGGTTGCAA
TAATTTTTGCACCTATTGCATAGTGCCATATACAAGAGGCAGAGAAAAGAGCAGAAAGCCAGAAGATATAATAGCAGAAG
TAAAAGAATTAGCCCAAAAAGGGTATAAGGAAATCACTTTATTGGGACAAAATGTAAATTCTTACGGAAAAGACCTTGAT
GAAGATATAACTTTTGCAAAACTACTTTATAAGCTAAATGATATTGAAGGAATTGAGAGAATAAGGTTTATGACTTCTCA
TCCTAAAGATATTTCCGATGAATTAATATATGCCATGAGGGATTTAGATAAAGTTTGTGAACATTTGCATTTACCTGTAC
AAGCTGGTAGCAACAAAATTTTAAAAAAGATGAATAGAAAATACACCAAAGAACACTATTTAGAAATTATTGATAAAGTA
AGAAGTAACATTCCAGATATTGCGATAACAACAGATATAATCGTAGGTTTTCCAGGTGAAACAGAAGAAGATTTTTTGGA
GACGTTGGATTTAGTAGAAAGAGTGCGATTTGATGCCGCTTATACTTTTATTTACTCTAAAAGAGCAGGCACTGTTGCGG
CAAATATGCCAGACCAAGTAGATGATGCTGTAAAACATGAGCGTTTAGAAAGACTTATAGAGTTGCAAAACAAAATCAGC
TTAGAAAAAAGTGCAGAATTGAGAGGAAAAATTGTGGAAGTATTGATAGAAGGAATTAGTAAAAGAGATAGTAATAAATT
GACCTCTCGAACTCGAACCAATAAAGTAGTCCACTTTGTGGGAGATGAAAGCCTTATCGGCAAACTTGCTAATGTTAAAA
TAACTGAAACAAAGGCTTGGACAATGCAGGGAGAATTAGTAGAAGTTATAAGATAA

Upstream 100 bases:

>100_bases
CAGAGCAAATAATATTGCCATATAGATACAATTTTGAACCAATTAAAACTTTTTTCTATAAAAAAATTATTCCACAGGGA
TAAAAGAGGTGTTAATAATA

Downstream 100 bases:

>100_bases
AAGTGCGCCTATATGGGCGCTAATTTTTATGATATAATAAAGAGGAGTCGGAGGTGCAAGAAAATGGCCGTCACTCCAAT
GATGGAACAATACCTTAAAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MPTNIYVTEEELKKQEKIMKEIAEENKGKNLYYHIETYGCQMNVHDSEKLAGMLEKMGYKYTENLEQADVLLFNTCAVRE
HAEIRVLGRVSQMKELKARNPNLIIGVSGCMMQEKNVVEAIKEKYSYIDIVFGTHNIYKFPQLLWEALNSQDIVIDIIED
TKNVIEELPVKRDSNLKAWVNIIYGCNNFCTYCIVPYTRGREKSRKPEDIIAEVKELAQKGYKEITLLGQNVNSYGKDLD
EDITFAKLLYKLNDIEGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKILKKMNRKYTKEHYLEIIDKV
RSNIPDIAITTDIIVGFPGETEEDFLETLDLVERVRFDAAYTFIYSKRAGTVAANMPDQVDDAVKHERLERLIELQNKIS
LEKSAELRGKIVEVLIEGISKRDSNKLTSRTRTNKVVHFVGDESLIGKLANVKITETKAWTMQGELVEVIR

Sequences:

>Translated_471_residues
MPTNIYVTEEELKKQEKIMKEIAEENKGKNLYYHIETYGCQMNVHDSEKLAGMLEKMGYKYTENLEQADVLLFNTCAVRE
HAEIRVLGRVSQMKELKARNPNLIIGVSGCMMQEKNVVEAIKEKYSYIDIVFGTHNIYKFPQLLWEALNSQDIVIDIIED
TKNVIEELPVKRDSNLKAWVNIIYGCNNFCTYCIVPYTRGREKSRKPEDIIAEVKELAQKGYKEITLLGQNVNSYGKDLD
EDITFAKLLYKLNDIEGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKILKKMNRKYTKEHYLEIIDKV
RSNIPDIAITTDIIVGFPGETEEDFLETLDLVERVRFDAAYTFIYSKRAGTVAANMPDQVDDAVKHERLERLIELQNKIS
LEKSAELRGKIVEVLIEGISKRDSNKLTSRTRTNKVVHFVGDESLIGKLANVKITETKAWTMQGELVEVIR
>Mature_470_residues
PTNIYVTEEELKKQEKIMKEIAEENKGKNLYYHIETYGCQMNVHDSEKLAGMLEKMGYKYTENLEQADVLLFNTCAVREH
AEIRVLGRVSQMKELKARNPNLIIGVSGCMMQEKNVVEAIKEKYSYIDIVFGTHNIYKFPQLLWEALNSQDIVIDIIEDT
KNVIEELPVKRDSNLKAWVNIIYGCNNFCTYCIVPYTRGREKSRKPEDIIAEVKELAQKGYKEITLLGQNVNSYGKDLDE
DITFAKLLYKLNDIEGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKILKKMNRKYTKEHYLEIIDKVR
SNIPDIAITTDIIVGFPGETEEDFLETLDLVERVRFDAAYTFIYSKRAGTVAANMPDQVDDAVKHERLERLIELQNKISL
EKSAELRGKIVEVLIEGISKRDSNKLTSRTRTNKVVHFVGDESLIGKLANVKITETKAWTMQGELVEVIR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=515, Percent_Identity=35.1456310679612, Blast_Score=308, Evalue=8e-84,
Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=36.1746361746362, Blast_Score=305, Evalue=6e-83,
Organism=Homo sapiens, GI93277076, Length=442, Percent_Identity=29.8642533936652, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1786882, Length=437, Percent_Identity=43.0205949656751, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=27.2727272727273, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=478, Percent_Identity=34.7280334728033, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=327, Percent_Identity=27.82874617737, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21356207, Length=484, Percent_Identity=34.9173553719008, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=28.6666666666667, Blast_Score=181, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 54299; Mature: 54168

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTNIYVTEEELKKQEKIMKEIAEENKGKNLYYHIETYGCQMNVHDSEKLAGMLEKMGYK
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCH
YTENLEQADVLLFNTCAVREHAEIRVLGRVSQMKELKARNPNLIIGVSGCMMQEKNVVEA
HHCCCCHHHEEEEEHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
IKEKYSYIDIVFGTHNIYKFPQLLWEALNSQDIVIDIIEDTKNVIEELPVKRDSNLKAWV
HHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCHHHHH
NIIYGCNNFCTYCIVPYTRGREKSRKPEDIIAEVKELAQKGYKEITLLGQNVNSYGKDLD
HHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCH
EDITFAKLLYKLNDIEGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCHHH
LKKMNRKYTKEHYLEIIDKVRSNIPDIAITTDIIVGFPGETEEDFLETLDLVERVRFDAA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
YTFIYSKRAGTVAANMPDQVDDAVKHERLERLIELQNKISLEKSAELRGKIVEVLIEGIS
HEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KRDSNKLTSRTRTNKVVHFVGDESLIGKLANVKITETKAWTMQGELVEVIR
CCCCHHHHHHHHCCCEEEEECCHHHHHHHHCCEEECCEEEEECHHHHHHCC
>Mature Secondary Structure 
PTNIYVTEEELKKQEKIMKEIAEENKGKNLYYHIETYGCQMNVHDSEKLAGMLEKMGYK
CCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHCCCH
YTENLEQADVLLFNTCAVREHAEIRVLGRVSQMKELKARNPNLIIGVSGCMMQEKNVVEA
HHCCCCHHHEEEEEHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
IKEKYSYIDIVFGTHNIYKFPQLLWEALNSQDIVIDIIEDTKNVIEELPVKRDSNLKAWV
HHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCHHHHH
NIIYGCNNFCTYCIVPYTRGREKSRKPEDIIAEVKELAQKGYKEITLLGQNVNSYGKDLD
HHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCH
EDITFAKLLYKLNDIEGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCHHH
LKKMNRKYTKEHYLEIIDKVRSNIPDIAITTDIIVGFPGETEEDFLETLDLVERVRFDAA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
YTFIYSKRAGTVAANMPDQVDDAVKHERLERLIELQNKISLEKSAELRGKIVEVLIEGIS
HEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KRDSNKLTSRTRTNKVVHFVGDESLIGKLANVKITETKAWTMQGELVEVIR
CCCCHHHHHHHHCCCEEEEECCHHHHHHHHCCEEECCEEEEECHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA