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The map label for this gene is sigF [H]

Identifier: 167040197

GI number: 167040197

Start: 1581037

End: 1581777

Strand: Direct

Name: sigF [H]

Synonym: Teth514_1559

Alternate gene names: 167040197

Gene position: 1581037-1581777 (Clockwise)

Preceding gene: 167040196

Following gene: 167040198

Centisome position: 64.34

GC content: 30.36

Gene sequence:

>741_bases
ATGAAAGAAGGGAATTTTGAAAAAAGTGATGAGAATTTGGAATTGATAAGAGCAGCACAAAAGAATGATAAAGAAAGCTT
GGAAAAATTAATTTTAGAAAATAGTGGCCTTATTTGGAGTATTGTTAAAAAATTTTCTAATAGAGGGTATGAATTGGAGG
ATTTATATCAAATAGGTTGTATAGGTTTTGTAAAGGCAATTCAAAAATTTGATGAATCTTACAATGTAAAATTGTCTACC
TATGCAGTACCTATAATAATGGGAGAAATTAAGAGATTTCTAAGAGATGATGGATTAATAAAAGTGAGTCGTTCTTTAAA
GGAGCTTTCTACAAAAGCTTTTTATGTTAAAGAGATTTTAAGCAATGAATTAAATAGAGAGCCAACTATTAATGAAATTG
CTGAAAGATTAAATGTAAGTCCTGAAGAAATTGCTATGGCTTTTGAATCGGCTGCTACTGCTGAGTCATTATATGACAAC
GCAACTCATGACGAAGATGACAGGCTTTTAATTGAAATGGTGAGTGAAGAGGAAAAAGAAGTTGATATAGACGATAAATT
AGCTTTACAGATGGTTATAAATAAACTTAAACCACGAGAAAAACAAATAATATTTTTGAGATATTTCAAGGATATGACGC
AGATGGAGGTGGCAAGTGTTTTAGGAATTTCCCAAGTGCAAGTATCTAGAATTGAGAAAAAAGTTTTACAAAAACTAAGA
AAAGAATTGGAAGAAGTGTAG

Upstream 100 bases:

>100_bases
GCAGACCTTTATGGATGAATTAGAAGTTGAATCAACTCCAGGAAAGGGAACTCTTGTCCGAATGACAAAATATATAGGGT
CTGATAAATGAGGTGACTTT

Downstream 100 bases:

>100_bases
TATTTTAACACAAATACTACACTTTTTTTGAATTAATTTTGCTTATCTTCACATACTAATAATGGCTGGTGAAATGAAAA
TGAAAAAGATGTTAATTATA

Product: FliA/WhiG family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKEGNFEKSDENLELIRAAQKNDKESLEKLILENSGLIWSIVKKFSNRGYELEDLYQIGCIGFVKAIQKFDESYNVKLST
YAVPIIMGEIKRFLRDDGLIKVSRSLKELSTKAFYVKEILSNELNREPTINEIAERLNVSPEEIAMAFESAATAESLYDN
ATHDEDDRLLIEMVSEEEKEVDIDDKLALQMVINKLKPREKQIIFLRYFKDMTQMEVASVLGISQVQVSRIEKKVLQKLR
KELEEV

Sequences:

>Translated_246_residues
MKEGNFEKSDENLELIRAAQKNDKESLEKLILENSGLIWSIVKKFSNRGYELEDLYQIGCIGFVKAIQKFDESYNVKLST
YAVPIIMGEIKRFLRDDGLIKVSRSLKELSTKAFYVKEILSNELNREPTINEIAERLNVSPEEIAMAFESAATAESLYDN
ATHDEDDRLLIEMVSEEEKEVDIDDKLALQMVINKLKPREKQIIFLRYFKDMTQMEVASVLGISQVQVSRIEKKVLQKLR
KELEEV
>Mature_246_residues
MKEGNFEKSDENLELIRAAQKNDKESLEKLILENSGLIWSIVKKFSNRGYELEDLYQIGCIGFVKAIQKFDESYNVKLST
YAVPIIMGEIKRFLRDDGLIKVSRSLKELSTKAFYVKEILSNELNREPTINEIAERLNVSPEEIAMAFESAATAESLYDN
ATHDEDDRLLIEMVSEEEKEVDIDDKLALQMVINKLKPREKQIIFLRYFKDMTQMEVASVLGISQVQVSRIEKKVLQKLR
KELEEV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. It is responsible for directing gene

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=233, Percent_Identity=30.0429184549356, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789098, Length=253, Percent_Identity=24.1106719367589, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788231, Length=201, Percent_Identity=26.865671641791, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28448; Mature: 28448

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEGNFEKSDENLELIRAAQKNDKESLEKLILENSGLIWSIVKKFSNRGYELEDLYQIGC
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
IGFVKAIQKFDESYNVKLSTYAVPIIMGEIKRFLRDDGLIKVSRSLKELSTKAFYVKEIL
HHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SNELNREPTINEIAERLNVSPEEIAMAFESAATAESLYDNATHDEDDRLLIEMVSEEEKE
HHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
VDIDDKLALQMVINKLKPREKQIIFLRYFKDMTQMEVASVLGISQVQVSRIEKKVLQKLR
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELEEV
HHHHCC
>Mature Secondary Structure
MKEGNFEKSDENLELIRAAQKNDKESLEKLILENSGLIWSIVKKFSNRGYELEDLYQIGC
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
IGFVKAIQKFDESYNVKLSTYAVPIIMGEIKRFLRDDGLIKVSRSLKELSTKAFYVKEIL
HHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SNELNREPTINEIAERLNVSPEEIAMAFESAATAESLYDNATHDEDDRLLIEMVSEEEKE
HHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
VDIDDKLALQMVINKLKPREKQIIFLRYFKDMTQMEVASVLGISQVQVSRIEKKVLQKLR
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELEEV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1391049 [H]