Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 167039953

GI number: 167039953

Start: 1347409

End: 1348698

Strand: Direct

Name: eno [H]

Synonym: Teth514_1310

Alternate gene names: 167039953

Gene position: 1347409-1348698 (Clockwise)

Preceding gene: 167039952

Following gene: 167039954

Centisome position: 54.83

GC content: 38.6

Gene sequence:

>1290_bases
ATGTCATCAATAATTGATATTTTTGCAAGAGAAATACTTGACTCCCGTGGAAATCCCACAGTTGAAGTAGAGGTAGAATT
AGATAGCGGTGCCGTAGGAAGAGCTGCTGTGCCTTCAGGGGCTTCTACAGGAGCTTTTGAAGCAGTTGAATTAAGAGATG
GAGACAAATCAAGGTATTTGGGAAAAGGAGTGCTAAAAGCTGTTCAAAATGTCAATGACATTATTGCTCCTGAGCTAATT
GGCATGGAAGCACAAGACCAAGTAGCAATTGACAAAGCGATGATTGAACTAGATGGAACTCCCAATAAGAGCAAATTAGG
AGCAAATGCTATCTTAGGGGTATCTTTGGCAGTGGCAAAGGCAGCAGCTGAGGAATGTGGTTTACCTTTGTATCAATACA
TTGGTGGAGTAAATGCAAAAACTCTTCCTGTGCCAATGATGAACATATTAAACGGTGGAAAACACGCTGATAACAATGTA
GATATACAAGAGTTTATGATAATGCCTGTTGGTGCTCCAAACTTCCGTGAAGCATTAAGAATGTGTTCAGAAGTTTATCA
TAATTTAAAAAACGTGTTACATTCTAAAGGGTTAAGTACAACAGTTGGTGATGAAGGAGGTTTTGCTCCCAACCTTACCT
CAAATGAAGAAGCTATACAAGTCATATTGGAAGCAATAGAAAAAGCAGGATATGTTCCGGGAGAAGATATTGTTTTAGCA
TTAGACCCTGCGGCTACTGAAATGTACAAAGAAGATGGGAAATATCATTTCGAAGGCGAAGGCATTGTAAGGACCTCGGC
TGAAATGGTAGATTTTTGGGAACAACTTGTTAACAAATATCCTATTGTCTCTATTGAAGACGGTCTTGCAGAAGAAGACT
GGAACGGATGGAAACTATTGACTGAAAGATTAGGCAAGAAGATACAGCTTGTAGGAGACGACTTATTTGTAACGAATACT
CAAAGACTTTCAAAAGGTATAAGCATGGGAGTAGCAAATTCAATCCTTATAAAACTCAATCAAATAGGAACTTTGACAGA
AACTCTTGATGCTATTGAAATGGCAAAAAGAGCAGGTTACACAGCTGTTGTGTCTCACCGCTCAGGAGAGACAGAAGATT
CTACAATTGCAGACCTTGTAGTTGGAGTAAATGCAGGGCAAATTAAAACTGGAGCACCTGCAAGAACTGATAGAGTCGCA
AAATACAATCAGCTTTTAAGAATTGAAGAAGTTTTAGGAAGCACAGCTCAATATCTTGGCAAAAATGCTTTTTACAATAT
TAAGAAATAA

Upstream 100 bases:

>100_bases
CTGTATTAGACATAATGAAATTGCCTATACCTCAAGAGATGACAGGTAAATCTTTAATTATTGGAAGAAAATAAAATTTA
AAAAGAAAGGAGAAGTTGTC

Downstream 100 bases:

>100_bases
GGGGATCCAAAAAGGATTCCCTTTTTATATAAAAACCTCTTGATCAACATAGCAATTCTTTGTATAATATAAACTGTGGT
ATTGTGTTTCAAAAATTAGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSIIDIFAREILDSRGNPTVEVEVELDSGAVGRAAVPSGASTGAFEAVELRDGDKSRYLGKGVLKAVQNVNDIIAPELI
GMEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAKAAAEECGLPLYQYIGGVNAKTLPVPMMNILNGGKHADNNV
DIQEFMIMPVGAPNFREALRMCSEVYHNLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIQVILEAIEKAGYVPGEDIVLA
LDPAATEMYKEDGKYHFEGEGIVRTSAEMVDFWEQLVNKYPIVSIEDGLAEEDWNGWKLLTERLGKKIQLVGDDLFVTNT
QRLSKGISMGVANSILIKLNQIGTLTETLDAIEMAKRAGYTAVVSHRSGETEDSTIADLVVGVNAGQIKTGAPARTDRVA
KYNQLLRIEEVLGSTAQYLGKNAFYNIKK

Sequences:

>Translated_429_residues
MSSIIDIFAREILDSRGNPTVEVEVELDSGAVGRAAVPSGASTGAFEAVELRDGDKSRYLGKGVLKAVQNVNDIIAPELI
GMEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAKAAAEECGLPLYQYIGGVNAKTLPVPMMNILNGGKHADNNV
DIQEFMIMPVGAPNFREALRMCSEVYHNLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIQVILEAIEKAGYVPGEDIVLA
LDPAATEMYKEDGKYHFEGEGIVRTSAEMVDFWEQLVNKYPIVSIEDGLAEEDWNGWKLLTERLGKKIQLVGDDLFVTNT
QRLSKGISMGVANSILIKLNQIGTLTETLDAIEMAKRAGYTAVVSHRSGETEDSTIADLVVGVNAGQIKTGAPARTDRVA
KYNQLLRIEEVLGSTAQYLGKNAFYNIKK
>Mature_428_residues
SSIIDIFAREILDSRGNPTVEVEVELDSGAVGRAAVPSGASTGAFEAVELRDGDKSRYLGKGVLKAVQNVNDIIAPELIG
MEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAKAAAEECGLPLYQYIGGVNAKTLPVPMMNILNGGKHADNNVD
IQEFMIMPVGAPNFREALRMCSEVYHNLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIQVILEAIEKAGYVPGEDIVLAL
DPAATEMYKEDGKYHFEGEGIVRTSAEMVDFWEQLVNKYPIVSIEDGLAEEDWNGWKLLTERLGKKIQLVGDDLFVTNTQ
RLSKGISMGVANSILIKLNQIGTLTETLDAIEMAKRAGYTAVVSHRSGETEDSTIADLVVGVNAGQIKTGAPARTDRVAK
YNQLLRIEEVLGSTAQYLGKNAFYNIKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=53.7037037037037, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=54.1666666666667, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=52.7649769585253, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=52.7649769585253, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=47.4537037037037, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.1904761904762, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.1904761904762, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.1904761904762, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=66.6666666666667, Blast_Score=541, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.2407407407407, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=53.2407407407407, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=46.3541666666667, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=441, Percent_Identity=51.4739229024943, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=50.5773672055427, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=50.5773672055427, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=50.1154734411085, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=441, Percent_Identity=50.3401360544218, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=53.3487297921478, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=53.3487297921478, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=53.3487297921478, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=53.3487297921478, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=53.3487297921478, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=53.3487297921478, Blast_Score=411, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46171; Mature: 46040

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIIDIFAREILDSRGNPTVEVEVELDSGAVGRAAVPSGASTGAFEAVELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
GKGVLKAVQNVNDIIAPELIGMEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAK
HHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHHHHH
AAAEECGLPLYQYIGGVNAKTLPVPMMNILNGGKHADNNVDIQEFMIMPVGAPNFREALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHHHHH
MCSEVYHNLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIQVILEAIEKAGYVPGEDIVLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDPAATEMYKEDGKYHFEGEGIVRTSAEMVDFWEQLVNKYPIVSIEDGLAEEDWNGWKLL
ECCHHHHHHHHCCCEEECCCCEEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
TERLGKKIQLVGDDLFVTNTQRLSKGISMGVANSILIKLNQIGTLTETLDAIEMAKRAGY
HHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHHCCC
TAVVSHRSGETEDSTIADLVVGVNAGQIKTGAPARTDRVAKYNQLLRIEEVLGSTAQYLG
EEEEECCCCCCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
KNAFYNIKK
CCCCEECCC
>Mature Secondary Structure 
SSIIDIFAREILDSRGNPTVEVEVELDSGAVGRAAVPSGASTGAFEAVELRDGDKSRYL
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
GKGVLKAVQNVNDIIAPELIGMEAQDQVAIDKAMIELDGTPNKSKLGANAILGVSLAVAK
HHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHEEEECCCCCHHHCCCHHHHHHHHHHHH
AAAEECGLPLYQYIGGVNAKTLPVPMMNILNGGKHADNNVDIQEFMIMPVGAPNFREALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHHHHH
MCSEVYHNLKNVLHSKGLSTTVGDEGGFAPNLTSNEEAIQVILEAIEKAGYVPGEDIVLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDPAATEMYKEDGKYHFEGEGIVRTSAEMVDFWEQLVNKYPIVSIEDGLAEEDWNGWKLL
ECCHHHHHHHHCCCEEECCCCEEEHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
TERLGKKIQLVGDDLFVTNTQRLSKGISMGVANSILIKLNQIGTLTETLDAIEMAKRAGY
HHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHHCCC
TAVVSHRSGETEDSTIADLVVGVNAGQIKTGAPARTDRVAKYNQLLRIEEVLGSTAQYLG
EEEEECCCCCCCCHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
KNAFYNIKK
CCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA