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The map label for this gene is nfsA [C]

Identifier: 167039852

GI number: 167039852

Start: 1247031

End: 1247528

Strand: Reverse

Name: nfsA [C]

Synonym: Teth514_1207

Alternate gene names: 167039852

Gene position: 1247528-1247031 (Counterclockwise)

Preceding gene: 167039854

Following gene: 167039851

Centisome position: 50.77

GC content: 34.94

Gene sequence:

>498_bases
ATGGAGGCATTAGAGGCTTTAAAAAAGAGAAGAAGTATAAGGAAATACATAGATAAACCTATTCCAAAAGAAATATTAGA
AGACATAATAGACTGCGCAAGGCTTGCTCCCAGTGGAAATAACGCACAACCTTGGCATTTTGTCGTTATAACAGACAAAG
AAAGACTTAAATTCATAGCAGAAAAAGCTACATATGGGAAATTCATTAAAGATGCAGGAGCTTGTGTCATCGTCTACTGC
GATAAAAATAACAGACATCATTTAGAAGATGGTTCAGCAGCAACAGAAAATATATTACTCGCAGCTACTGCCTACGGCTT
AGGTTCCTGTTGGGTAGCTGGGTATAACAGAACCTATGAAAAAGAAATAAACGAATACCTCAACATTCCTGACAACTTAA
GAATGATTTCTATTATATCATTAGGATATCCCGCAGAAGAACCACAACCAAAATATAAAAAAGCTTTATCTGAAGTTTTA
CATTGGGAAAAATTTTAA

Upstream 100 bases:

>100_bases
TAAGTTTACTTAGCTTTATTTTTACATACCCATAAATTTTTGTCAATCTTTTTGGAATTTTTACTTTATGGGATATAATA
TAAAGAGGAGGTGCGAAATT

Downstream 100 bases:

>100_bases
ATGAAAGAGGGTTATAATATGTCAAAAATAGTGGAGAGATTTTTAAAATATGTAAAATATCATTCAACATCGGATGAAAA
CTCAACAACTTATCCCAGCA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MEALEALKKRRSIRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFVVITDKERLKFIAEKATYGKFIKDAGACVIVYC
DKNNRHHLEDGSAATENILLAATAYGLGSCWVAGYNRTYEKEINEYLNIPDNLRMISIISLGYPAEEPQPKYKKALSEVL
HWEKF

Sequences:

>Translated_165_residues
MEALEALKKRRSIRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFVVITDKERLKFIAEKATYGKFIKDAGACVIVYC
DKNNRHHLEDGSAATENILLAATAYGLGSCWVAGYNRTYEKEINEYLNIPDNLRMISIISLGYPAEEPQPKYKKALSEVL
HWEKF
>Mature_165_residues
MEALEALKKRRSIRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFVVITDKERLKFIAEKATYGKFIKDAGACVIVYC
DKNNRHHLEDGSAATENILLAATAYGLGSCWVAGYNRTYEKEINEYLNIPDNLRMISIISLGYPAEEPQPKYKKALSEVL
HWEKF

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 18845; Mature: 18845

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEALEALKKRRSIRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFVVITDKERLKFIA
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHHH
EKATYGKFIKDAGACVIVYCDKNNRHHLEDGSAATENILLAATAYGLGSCWVAGYNRTYE
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEEHHCCCCHHEECCCCCHHH
KEINEYLNIPDNLRMISIISLGYPAEEPQPKYKKALSEVLHWEKF
HHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEALEALKKRRSIRKYIDKPIPKEILEDIIDCARLAPSGNNAQPWHFVVITDKERLKFIA
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHHH
EKATYGKFIKDAGACVIVYCDKNNRHHLEDGSAATENILLAATAYGLGSCWVAGYNRTYE
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEEHHCCCCHHEECCCCCHHH
KEINEYLNIPDNLRMISIISLGYPAEEPQPKYKKALSEVLHWEKF
HHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]