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The map label for this gene is thiC [H]

Identifier: 167039626

GI number: 167039626

Start: 993893

End: 995191

Strand: Reverse

Name: thiC [H]

Synonym: Teth514_0974

Alternate gene names: 167039626

Gene position: 995191-993893 (Counterclockwise)

Preceding gene: 167039646

Following gene: 167039625

Centisome position: 40.5

GC content: 39.8

Gene sequence:

>1299_bases
ATGACACAATTGGAATACGCCCTTTCAGGCATTGTCACAAAAGAGATGAGAATAGTTGCAGAGTATGAAGGAGTAGACGA
AGAATTTATTTTAGAAGGAGTTAAAAAAGGAGAAATAGTAATACCATCAAACATCAACCACAAAAACCTCATTCCCAAAG
GCATAGGAAGGGGTTTATCGACAAAAGTAAATGCCAATATAGGAACCTCTGATGCATACCCTGAAATTGAAAAAGAAATT
GAAAAATTAAATGTTGCTGTAAAAGCTGGAGCAGATGCGGTAATGGATTTAAGCACAGGGGGCGACATTAACCAATCTCG
TAGAAAAATACTTGAAAATTCCCCTGTCCCTGTAGGCACTGTCCCCATGTATCAAGCAGCTGTAGAATCTATATCCAAAT
ACGGTAGCATTGTAGCTATGCCTGAAGAATTCATTTTTGAAGTTATAGAAGAACAAGCAAAAGACGGGGTTGATTTTATT
ACAGTCCACTGTGGTCTAACATTTGAATCATTGAAAAAGCTTAAAGACAACGGCCGAGTGATGGATATAGTAAGCCGCGG
TGGCTCCTTTACAATTGCTTGGATGCTCCATAACGACAAAGAAAATCCTTTGTATAAACATTTTGATAGGCTCCTTGATA
TTGCTAAAAAATATGACATAACTCTAAGCTTAGGAGATGGACTGCGTCCAGGTTGTCTCGAAGACGCTACAGATAGCGCA
CAAATTCAAGAGCTCATCATCCTTGGGGAACTTGTCAAAAGGGCTCGTAAAGCAGGAGTTCAAGTGATGGTAGAAGGACC
CGGGCATGTGCCAATTGACCAAATTGAAGCAAATGTAAAACTTCAAAAACAACTTTGTCATAATGCTCCTTTTTATGTGC
TTGGCCCTATTGTGACTGATATAGCTCCTGGTTATGACCACATAACTTCAGCAATCGGAGGAGCAATTGCAGCAGCTTCT
GGTGCTGATTTCCTTTGCTATGTTACACCCGCTGAACATCTCGGACTTCCAGACAAAGAAGATGTCAAAGAAGGCGTTAT
TGCAGCAAAAATTGCCGCCCATGCTGCAGATATCGCAAAAGGCGTAAAAGGTGCTAAAGAAAAAGATTTAACTATGGCTA
GAGCTAGAAAAGCCTTAAACTGGGATGAGCAAATAAAGCTTTCTATAGACCCTGATAAAGCTTTTAAATATCGCATCAAT
AAAAACATATCTACAGCCAAAACTTGCAGTATGTGCGGAAAATTCTGCGCTATGAAAATTGTCAGTGAGTACCTTGGAAC
TTCAGCTATGACTTGCTAA

Upstream 100 bases:

>100_bases
AACCCATCGAACCTGTTAGGTTAGTACCTGCGTAGGGATGTGGTCATCTGCCATGGCTATAATCCCCATGGTTTATTTAT
TTTTTAAGGAGGTTTTTATT

Downstream 100 bases:

>100_bases
TACCTTTGCCTTATTTACATTCCCCCCTTGATTTTCTTTGCTAATTTCTGCTATAATAAAGGAAAACATCAAGGGGGTAA
TTTTTATGGAAAAGTGGCAG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTQLEYALSGIVTKEMRIVAEYEGVDEEFILEGVKKGEIVIPSNINHKNLIPKGIGRGLSTKVNANIGTSDAYPEIEKEI
EKLNVAVKAGADAVMDLSTGGDINQSRRKILENSPVPVGTVPMYQAAVESISKYGSIVAMPEEFIFEVIEEQAKDGVDFI
TVHCGLTFESLKKLKDNGRVMDIVSRGGSFTIAWMLHNDKENPLYKHFDRLLDIAKKYDITLSLGDGLRPGCLEDATDSA
QIQELIILGELVKRARKAGVQVMVEGPGHVPIDQIEANVKLQKQLCHNAPFYVLGPIVTDIAPGYDHITSAIGGAIAAAS
GADFLCYVTPAEHLGLPDKEDVKEGVIAAKIAAHAADIAKGVKGAKEKDLTMARARKALNWDEQIKLSIDPDKAFKYRIN
KNISTAKTCSMCGKFCAMKIVSEYLGTSAMTC

Sequences:

>Translated_432_residues
MTQLEYALSGIVTKEMRIVAEYEGVDEEFILEGVKKGEIVIPSNINHKNLIPKGIGRGLSTKVNANIGTSDAYPEIEKEI
EKLNVAVKAGADAVMDLSTGGDINQSRRKILENSPVPVGTVPMYQAAVESISKYGSIVAMPEEFIFEVIEEQAKDGVDFI
TVHCGLTFESLKKLKDNGRVMDIVSRGGSFTIAWMLHNDKENPLYKHFDRLLDIAKKYDITLSLGDGLRPGCLEDATDSA
QIQELIILGELVKRARKAGVQVMVEGPGHVPIDQIEANVKLQKQLCHNAPFYVLGPIVTDIAPGYDHITSAIGGAIAAAS
GADFLCYVTPAEHLGLPDKEDVKEGVIAAKIAAHAADIAKGVKGAKEKDLTMARARKALNWDEQIKLSIDPDKAFKYRIN
KNISTAKTCSMCGKFCAMKIVSEYLGTSAMTC
>Mature_431_residues
TQLEYALSGIVTKEMRIVAEYEGVDEEFILEGVKKGEIVIPSNINHKNLIPKGIGRGLSTKVNANIGTSDAYPEIEKEIE
KLNVAVKAGADAVMDLSTGGDINQSRRKILENSPVPVGTVPMYQAAVESISKYGSIVAMPEEFIFEVIEEQAKDGVDFIT
VHCGLTFESLKKLKDNGRVMDIVSRGGSFTIAWMLHNDKENPLYKHFDRLLDIAKKYDITLSLGDGLRPGCLEDATDSAQ
IQELIILGELVKRARKAGVQVMVEGPGHVPIDQIEANVKLQKQLCHNAPFYVLGPIVTDIAPGYDHITSAIGGAIAAASG
ADFLCYVTPAEHLGLPDKEDVKEGVIAAKIAAHAADIAKGVKGAKEKDLTMARARKALNWDEQIKLSIDPDKAFKYRINK
NISTAKTCSMCGKFCAMKIVSEYLGTSAMTC

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=455, Percent_Identity=47.2527472527472, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 46895; Mature: 46763

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLEYALSGIVTKEMRIVAEYEGVDEEFILEGVKKGEIVIPSNINHKNLIPKGIGRGLS
CCHHHHHHHHHHHHHHHEEHHHCCCCHHHHHHCCCCCCEEECCCCCCCCCCCHHHCCCCC
TKVNANIGTSDAYPEIEKEIEKLNVAVKAGADAVMDLSTGGDINQSRRKILENSPVPVGT
EEECCCCCCCCCCHHHHHHHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHCCCCCCCCC
VPMYQAAVESISKYGSIVAMPEEFIFEVIEEQAKDGVDFITVHCGLTFESLKKLKDNGRV
CHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCE
MDIVSRGGSFTIAWMLHNDKENPLYKHFDRLLDIAKKYDITLSLGDGLRPGCLEDATDSA
EEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCHH
QIQELIILGELVKRARKAGVQVMVEGPGHVPIDQIEANVKLQKQLCHNAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHCCCCCEEECHHHHH
IAPGYDHITSAIGGAIAAASGADFLCYVTPAEHLGLPDKEDVKEGVIAAKIAAHAADIAK
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVKGAKEKDLTMARARKALNWDEQIKLSIDPDKAFKYRINKNISTAKTCSMCGKFCAMKI
HHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VSEYLGTSAMTC
HHHHHCCCCCCC
>Mature Secondary Structure 
TQLEYALSGIVTKEMRIVAEYEGVDEEFILEGVKKGEIVIPSNINHKNLIPKGIGRGLS
CHHHHHHHHHHHHHHHEEHHHCCCCHHHHHHCCCCCCEEECCCCCCCCCCCHHHCCCCC
TKVNANIGTSDAYPEIEKEIEKLNVAVKAGADAVMDLSTGGDINQSRRKILENSPVPVGT
EEECCCCCCCCCCHHHHHHHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHCCCCCCCCC
VPMYQAAVESISKYGSIVAMPEEFIFEVIEEQAKDGVDFITVHCGLTFESLKKLKDNGRV
CHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCE
MDIVSRGGSFTIAWMLHNDKENPLYKHFDRLLDIAKKYDITLSLGDGLRPGCLEDATDSA
EEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCHH
QIQELIILGELVKRARKAGVQVMVEGPGHVPIDQIEANVKLQKQLCHNAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHCCCCCEEECHHHHH
IAPGYDHITSAIGGAIAAASGADFLCYVTPAEHLGLPDKEDVKEGVIAAKIAAHAADIAK
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVKGAKEKDLTMARARKALNWDEQIKLSIDPDKAFKYRINKNISTAKTCSMCGKFCAMKI
HHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VSEYLGTSAMTC
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA