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The map label for this gene is hyfB [H]

Identifier: 167039570

GI number: 167039570

Start: 940842

End: 942521

Strand: Direct

Name: hyfB [H]

Synonym: Teth514_0918

Alternate gene names: 167039570

Gene position: 940842-942521 (Clockwise)

Preceding gene: 167039569

Following gene: 167039571

Centisome position: 38.29

GC content: 36.19

Gene sequence:

>1680_bases
ATGAGCTCATTGAGCTTACTTTTTATATTGGTACTCCCAATTATAGGTACGCTGGTAATATGGCCTGCCAGTAAAAAAAA
TGATAAGATGGGGGAGATTTTTGTCGCATTGATATGTGGCATCTGTGTATTATTTGCAGCATTTACTCCAGCAGGAGATT
TTTTGAGTATAAACACAGGTTTTCACTCAATTAGGTTTACTTTAGGTGATATAAATCGCATATTCCTGCTTTTTTCCACC
TCTATTTGGTGTTTAGTTGCACTTTTTCTGACGGGCTATGTTCATCATATGGAAAATAGGGCAAGGTTCTGGGGCTTTTT
CATGCTAACGTTATGGTCGGTTATATGTGTTTTTTTAGCTGGAGATCTTTTGACCTTTTACTTTTTTTTCGAATTAATGG
CTTTATTTTCTTATTTCTTAATTATACACCAAGAGTCAACTGAAGCCCTATCGTCAGGGAATTTATATATCTTTACCACA
TTTGTAGGGGGTCTTGCAGTTCTAGGTGCCATACTGATAATATACAATAATGTTGGAACTTTTGAGTTTGAAAAATTAAA
CCTTGTCATAGAACATGGGGGATTAAAGGGGTATTTGGCCCTCGTATTATTATTTGTAGGATTCGGGATTAAAGCAGGTT
GCCTGCCTTTTCATTTTTGGCTCCCAAGAGCTCATCCAGTAGCGCCGGCTCCAGCCAGTGCCTTGCTCTCTGGGGTACTT
GTGAAATCAGGAGTCTTTGGCATTCTGCAGATTTTGCGATATACAAATTCGCATTATTTAGGGCTGGTCCTGATAATTTT
AGGAATTTTGTCAACATTATACGGCGGTTTTATGGCTCTGTGTGACAATTACCCCAAGCGTCTTTTAGCATATGGTACTA
TAAGCCAGATAGGATATATTTTTGTAAGTATTAGTGCAACGATTTTAGCAGGGGCACATTCTTTGGTCGCTCCTTACTTG
CACATATATGCTCACGGTCTTTCTAAAACAGCATTGTTTCTTGTTGTTGGGTATATATATTTAAAGACTTATGAAATGAA
ACAACTAAAAAATCTTACTTTAGTCCCGCTGGCAGCAATTGCACTTGCGGGACTAAATCTCGCGGGATTCCCGGGATTTA
TAGGTTATACCGCAAAAATTTTAACAAAAGATTTACTTCTACGTCTTTCGACTCAAGGAATGTTTTTTCATTGGGTGGAG
GTAATTTTTAGACTTTCTGGCATTCTGACGGCGGCTTATCTAACCAAGATCTTTTTCTGCTTTTATGAGTCTTACTTAAA
ACACAGGGTAAAAAATCCATTGAGTAATCAAAAAGAGTTTAATATAATGATATTCGTGCTGTTTTTGTTGGTTTTTATGA
CAATCATCCTTGGATTCTTACCAATTGCGGCTAGCGATGTTGAAATTAATCTATATTCTCCAGCTAAAATAATTGCATCG
ATATTTGATATTATTACAGGTATTATAGTTTATTTGAAGTTCAAAGGTCACATAAAAAGACCTGTATTAATACCACAGCC
TTCATACAGAAATACAGTTTTCAAACTTACCGGTTATTTTGGTAATTTAGTCCAAAGGTATATTCATCTTCCTATAAGTA
TAGATCTCAGCATTGCGCTTTTTGGCATCTCAATTCCGTTGCTGTATTTACTGTTAATATTATACCTAAAGCCTTATTGA

Upstream 100 bases:

>100_bases
CCATTCTTATATTTACTTTTGTTATCATGTTTTTAGGCATTTATCCTAAATTTTTGTTAAAATATTTGTTGCAGAACATG
TTCTAAGGAGAGTGAAAGCA

Downstream 100 bases:

>100_bases
GTTTGAAAAAACGAGGAGGTTAGAGCCTTATGTTAAACTTTTGGGAAAAGTTCAAGTGGCGATTGCCCAAAAATTTTGCG
AGGCTAGTGTTTTTTTTGGA

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MSSLSLLFILVLPIIGTLVIWPASKKNDKMGEIFVALICGICVLFAAFTPAGDFLSINTGFHSIRFTLGDINRIFLLFST
SIWCLVALFLTGYVHHMENRARFWGFFMLTLWSVICVFLAGDLLTFYFFFELMALFSYFLIIHQESTEALSSGNLYIFTT
FVGGLAVLGAILIIYNNVGTFEFEKLNLVIEHGGLKGYLALVLLFVGFGIKAGCLPFHFWLPRAHPVAPAPASALLSGVL
VKSGVFGILQILRYTNSHYLGLVLIILGILSTLYGGFMALCDNYPKRLLAYGTISQIGYIFVSISATILAGAHSLVAPYL
HIYAHGLSKTALFLVVGYIYLKTYEMKQLKNLTLVPLAAIALAGLNLAGFPGFIGYTAKILTKDLLLRLSTQGMFFHWVE
VIFRLSGILTAAYLTKIFFCFYESYLKHRVKNPLSNQKEFNIMIFVLFLLVFMTIILGFLPIAASDVEINLYSPAKIIAS
IFDIITGIIVYLKFKGHIKRPVLIPQPSYRNTVFKLTGYFGNLVQRYIHLPISIDLSIALFGISIPLLYLLLILYLKPY

Sequences:

>Translated_559_residues
MSSLSLLFILVLPIIGTLVIWPASKKNDKMGEIFVALICGICVLFAAFTPAGDFLSINTGFHSIRFTLGDINRIFLLFST
SIWCLVALFLTGYVHHMENRARFWGFFMLTLWSVICVFLAGDLLTFYFFFELMALFSYFLIIHQESTEALSSGNLYIFTT
FVGGLAVLGAILIIYNNVGTFEFEKLNLVIEHGGLKGYLALVLLFVGFGIKAGCLPFHFWLPRAHPVAPAPASALLSGVL
VKSGVFGILQILRYTNSHYLGLVLIILGILSTLYGGFMALCDNYPKRLLAYGTISQIGYIFVSISATILAGAHSLVAPYL
HIYAHGLSKTALFLVVGYIYLKTYEMKQLKNLTLVPLAAIALAGLNLAGFPGFIGYTAKILTKDLLLRLSTQGMFFHWVE
VIFRLSGILTAAYLTKIFFCFYESYLKHRVKNPLSNQKEFNIMIFVLFLLVFMTIILGFLPIAASDVEINLYSPAKIIAS
IFDIITGIIVYLKFKGHIKRPVLIPQPSYRNTVFKLTGYFGNLVQRYIHLPISIDLSIALFGISIPLLYLLLILYLKPY
>Mature_558_residues
SSLSLLFILVLPIIGTLVIWPASKKNDKMGEIFVALICGICVLFAAFTPAGDFLSINTGFHSIRFTLGDINRIFLLFSTS
IWCLVALFLTGYVHHMENRARFWGFFMLTLWSVICVFLAGDLLTFYFFFELMALFSYFLIIHQESTEALSSGNLYIFTTF
VGGLAVLGAILIIYNNVGTFEFEKLNLVIEHGGLKGYLALVLLFVGFGIKAGCLPFHFWLPRAHPVAPAPASALLSGVLV
KSGVFGILQILRYTNSHYLGLVLIILGILSTLYGGFMALCDNYPKRLLAYGTISQIGYIFVSISATILAGAHSLVAPYLH
IYAHGLSKTALFLVVGYIYLKTYEMKQLKNLTLVPLAAIALAGLNLAGFPGFIGYTAKILTKDLLLRLSTQGMFFHWVEV
IFRLSGILTAAYLTKIFFCFYESYLKHRVKNPLSNQKEFNIMIFVLFLLVFMTIILGFLPIAASDVEINLYSPAKIIASI
FDIITGIIVYLKFKGHIKRPVLIPQPSYRNTVFKLTGYFGNLVQRYIHLPISIDLSIALFGISIPLLYLLLILYLKPY

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=365, Percent_Identity=26.027397260274, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI251831117, Length=352, Percent_Identity=25.8522727272727, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788827, Length=396, Percent_Identity=29.7979797979798, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788613, Length=301, Percent_Identity=31.5614617940199, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1788831, Length=325, Percent_Identity=29.5384615384615, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1788829, Length=395, Percent_Identity=27.8481012658228, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788614, Length=306, Percent_Identity=30.0653594771242, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI2367154, Length=346, Percent_Identity=28.9017341040462, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693160, Length=226, Percent_Identity=27.8761061946903, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 62426; Mature: 62295

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSLLFILVLPIIGTLVIWPASKKNDKMGEIFVALICGICVLFAAFTPAGDFLSINTG
CCCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
FHSIRFTLGDINRIFLLFSTSIWCLVALFLTGYVHHMENRARFWGFFMLTLWSVICVFLA
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDLLTFYFFFELMALFSYFLIIHQESTEALSSGNLYIFTTFVGGLAVLGAILIIYNNVGT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCC
FEFEKLNLVIEHGGLKGYLALVLLFVGFGIKAGCLPFHFWLPRAHPVAPAPASALLSGVL
EEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHH
VKSGVFGILQILRYTNSHYLGLVLIILGILSTLYGGFMALCDNYPKRLLAYGTISQIGYI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FVSISATILAGAHSLVAPYLHIYAHGLSKTALFLVVGYIYLKTYEMKQLKNLTLVPLAAI
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALAGLNLAGFPGFIGYTAKILTKDLLLRLSTQGMFFHWVEVIFRLSGILTAAYLTKIFFC
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYESYLKHRVKNPLSNQKEFNIMIFVLFLLVFMTIILGFLPIAASDVEINLYSPAKIIAS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHH
IFDIITGIIVYLKFKGHIKRPVLIPQPSYRNTVFKLTGYFGNLVQRYIHLPISIDLSIAL
HHHHHHHHHHHHEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHH
FGISIPLLYLLLILYLKPY
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSLSLLFILVLPIIGTLVIWPASKKNDKMGEIFVALICGICVLFAAFTPAGDFLSINTG
CCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
FHSIRFTLGDINRIFLLFSTSIWCLVALFLTGYVHHMENRARFWGFFMLTLWSVICVFLA
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDLLTFYFFFELMALFSYFLIIHQESTEALSSGNLYIFTTFVGGLAVLGAILIIYNNVGT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCC
FEFEKLNLVIEHGGLKGYLALVLLFVGFGIKAGCLPFHFWLPRAHPVAPAPASALLSGVL
EEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHH
VKSGVFGILQILRYTNSHYLGLVLIILGILSTLYGGFMALCDNYPKRLLAYGTISQIGYI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
FVSISATILAGAHSLVAPYLHIYAHGLSKTALFLVVGYIYLKTYEMKQLKNLTLVPLAAI
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALAGLNLAGFPGFIGYTAKILTKDLLLRLSTQGMFFHWVEVIFRLSGILTAAYLTKIFFC
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYESYLKHRVKNPLSNQKEFNIMIFVLFLLVFMTIILGFLPIAASDVEINLYSPAKIIAS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHH
IFDIITGIIVYLKFKGHIKRPVLIPQPSYRNTVFKLTGYFGNLVQRYIHLPISIDLSIAL
HHHHHHHHHHHHEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHH
FGISIPLLYLLLILYLKPY
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]