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The map label for this gene is ytsJ [H]

Identifier: 167039484

GI number: 167039484

Start: 867028

End: 868239

Strand: Direct

Name: ytsJ [H]

Synonym: Teth514_0830

Alternate gene names: 167039484

Gene position: 867028-868239 (Clockwise)

Preceding gene: 167039483

Following gene: 167039485

Centisome position: 35.28

GC content: 34.24

Gene sequence:

>1212_bases
ATGAATATAAGAGAAGAAACTTTAAAACTTCACAAAGAAAATAGAGGCAAACTTGAAATTGTAAGCAAAGTAGAAGTGAA
AAATGACAAAGATTTAGCTTTAGCCTATACCCCAGGTGTTGCGGAACCTTGCAAAGAGATACATAAAAATAGCGATTTAG
TATATGAATATACTACTAAAAGCCACATGATTGCAGTTGTCACAGATGGTTCTGCTGTTTTAGGATTAGGGAATATAGGA
CCGGAGGCAGCTCTTCCTGTTATGGAAGGAAAAGCAGTTTTATTTAAAGAATTTGGAGGCGTTGATGCAGTACCTATATG
TCTTGCTACTCAAGATGTTGAAGAAATTATAAAAACTGTGGTGAATATTTCACCTGCTTTTGGAGGCATAAATCTTGAAG
ATATTTCTGCACCTCGCTGTTTTGAAATAGAAAAAAAGTTGGACGAAATTTTGGATATACCCGTTTTTCATGATGATCAG
CATGGAACGGCAGTTGTAACTTTGGCTGCTTTAATAAATGCTTTAAAAATTGTAGAAAAGGAATTAAAAGAAGTTACTGC
CGTTGTAAATGGAGCTGGAGCGGCAGGGATTGCTATTGCTAAATTTTTAATAAAAGCGGGTATAAAAGATGTAATAGTTT
GCGATAGAAGTGGTATCATATATGAAGGAAGAGAAGATAAAGACTTCTCTAAAAAAGAGATTGCAAAAATATCAAATAAA
GGAAGATTAAGAGGGGCTTTAAAAGATGCTTTAAAAGGAGCAGATGTGTTTATTGGAGTATCAGCACCTAATGTATTAAA
TAAAGAAATGATAAAAACAATGGCCAAAAAACCAATAGTTTTCGCATTAGCTAATCCAGTACCTGAAATATATCCTGATG
AAGCAAAAGAGGCAGGAGCTTATGTGGTAGGTACTGGTAGATCTGATTTTCCAAACCAAATAAACAACGTGTTGGCTTTT
CCAGGTATATTTAGAGGGGCATTAGAAGTTAGGGCTACTACAATTAATGAAGAGATGAAAATTAGTGCTGCATATTCTAT
TGCCCAAACTATTTCAGAAAATGAACTTACTCCGGAGTATATAATTCCAAAGCCTTTTGATAAAAGAGTTCTTAAAAATG
TAGCAATAGCAGTTGCAAAAGCAGCAATAGACACGGATGTAGCTAGAATAAATGTGGATTTAAAAGAGATTGAGAAGAGT
TTAAGTGAATAA

Upstream 100 bases:

>100_bases
ATTATTCAGCTCCTACTCGATTGCAGCTAATAACTTTTTTCTAAAAAATATTTAAGTTATAATATAAGAAAACACGGGTA
TTAGAGGAGGACTTTATGAA

Downstream 100 bases:

>100_bases
GAAAAGTAAATAAGAGGTGGAGGAAATGAATAAAAATCAAAAATTCTTCAAAGGAAAATTGTACAAATCTATAAGAGGGA
ATTTTTTTGAAGAAGATACT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MNIREETLKLHKENRGKLEIVSKVEVKNDKDLALAYTPGVAEPCKEIHKNSDLVYEYTTKSHMIAVVTDGSAVLGLGNIG
PEAALPVMEGKAVLFKEFGGVDAVPICLATQDVEEIIKTVVNISPAFGGINLEDISAPRCFEIEKKLDEILDIPVFHDDQ
HGTAVVTLAALINALKIVEKELKEVTAVVNGAGAAGIAIAKFLIKAGIKDVIVCDRSGIIYEGREDKDFSKKEIAKISNK
GRLRGALKDALKGADVFIGVSAPNVLNKEMIKTMAKKPIVFALANPVPEIYPDEAKEAGAYVVGTGRSDFPNQINNVLAF
PGIFRGALEVRATTINEEMKISAAYSIAQTISENELTPEYIIPKPFDKRVLKNVAIAVAKAAIDTDVARINVDLKEIEKS
LSE

Sequences:

>Translated_403_residues
MNIREETLKLHKENRGKLEIVSKVEVKNDKDLALAYTPGVAEPCKEIHKNSDLVYEYTTKSHMIAVVTDGSAVLGLGNIG
PEAALPVMEGKAVLFKEFGGVDAVPICLATQDVEEIIKTVVNISPAFGGINLEDISAPRCFEIEKKLDEILDIPVFHDDQ
HGTAVVTLAALINALKIVEKELKEVTAVVNGAGAAGIAIAKFLIKAGIKDVIVCDRSGIIYEGREDKDFSKKEIAKISNK
GRLRGALKDALKGADVFIGVSAPNVLNKEMIKTMAKKPIVFALANPVPEIYPDEAKEAGAYVVGTGRSDFPNQINNVLAF
PGIFRGALEVRATTINEEMKISAAYSIAQTISENELTPEYIIPKPFDKRVLKNVAIAVAKAAIDTDVARINVDLKEIEKS
LSE
>Mature_403_residues
MNIREETLKLHKENRGKLEIVSKVEVKNDKDLALAYTPGVAEPCKEIHKNSDLVYEYTTKSHMIAVVTDGSAVLGLGNIG
PEAALPVMEGKAVLFKEFGGVDAVPICLATQDVEEIIKTVVNISPAFGGINLEDISAPRCFEIEKKLDEILDIPVFHDDQ
HGTAVVTLAALINALKIVEKELKEVTAVVNGAGAAGIAIAKFLIKAGIKDVIVCDRSGIIYEGREDKDFSKKEIAKISNK
GRLRGALKDALKGADVFIGVSAPNVLNKEMIKTMAKKPIVFALANPVPEIYPDEAKEAGAYVVGTGRSDFPNQINNVLAF
PGIFRGALEVRATTINEEMKISAAYSIAQTISENELTPEYIIPKPFDKRVLKNVAIAVAKAAIDTDVARINVDLKEIEKS
LSE

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=432, Percent_Identity=25.2314814814815, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI62420882, Length=438, Percent_Identity=24.8858447488584, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI62420880, Length=438, Percent_Identity=24.8858447488584, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI239049447, Length=438, Percent_Identity=24.8858447488584, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4505145, Length=433, Percent_Identity=24.7113163972286, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI270265879, Length=374, Percent_Identity=25.668449197861, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788806, Length=405, Percent_Identity=48.1481481481481, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6322823, Length=339, Percent_Identity=27.1386430678466, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI78707242, Length=401, Percent_Identity=25.1870324189526, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI78707238, Length=401, Percent_Identity=25.1870324189526, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI281362672, Length=453, Percent_Identity=24.5033112582781, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21356279, Length=453, Percent_Identity=24.5033112582781, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI281362674, Length=453, Percent_Identity=24.5033112582781, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281363505, Length=442, Percent_Identity=25.5656108597285, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI78707236, Length=442, Percent_Identity=25.5656108597285, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281363503, Length=442, Percent_Identity=25.5656108597285, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI78707232, Length=433, Percent_Identity=25.4041570438799, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI78707240, Length=358, Percent_Identity=25.1396648044693, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 43557; Mature: 43557

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIREETLKLHKENRGKLEIVSKVEVKNDKDLALAYTPGVAEPCKEIHKNSDLVYEYTTK
CCCHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECC
SHMIAVVTDGSAVLGLGNIGPEAALPVMEGKAVLFKEFGGVDAVPICLATQDVEEIIKTV
CEEEEEEECCCEEEEECCCCCHHCCCHHCCCEEEEHHCCCCCCEEEEEEHHHHHHHHHHH
VNISPAFGGINLEDISAPRCFEIEKKLDEILDIPVFHDDQHGTAVVTLAALINALKIVEK
HCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHH
ELKEVTAVVNGAGAAGIAIAKFLIKAGIKDVIVCDRSGIIYEGREDKDFSKKEIAKISNK
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHCC
GRLRGALKDALKGADVFIGVSAPNVLNKEMIKTMAKKPIVFALANPVPEIYPDEAKEAGA
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHCCC
YVVGTGRSDFPNQINNVLAFPGIFRGALEVRATTINEEMKISAAYSIAQTISENELTPEY
EEEECCCCCCCHHHHHHHHCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCE
IIPKPFDKRVLKNVAIAVAKAAIDTDVARINVDLKEIEKSLSE
ECCCCHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHHCC
>Mature Secondary Structure
MNIREETLKLHKENRGKLEIVSKVEVKNDKDLALAYTPGVAEPCKEIHKNSDLVYEYTTK
CCCHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECC
SHMIAVVTDGSAVLGLGNIGPEAALPVMEGKAVLFKEFGGVDAVPICLATQDVEEIIKTV
CEEEEEEECCCEEEEECCCCCHHCCCHHCCCEEEEHHCCCCCCEEEEEEHHHHHHHHHHH
VNISPAFGGINLEDISAPRCFEIEKKLDEILDIPVFHDDQHGTAVVTLAALINALKIVEK
HCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHH
ELKEVTAVVNGAGAAGIAIAKFLIKAGIKDVIVCDRSGIIYEGREDKDFSKKEIAKISNK
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHCC
GRLRGALKDALKGADVFIGVSAPNVLNKEMIKTMAKKPIVFALANPVPEIYPDEAKEAGA
CHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHCCC
YVVGTGRSDFPNQINNVLAFPGIFRGALEVRATTINEEMKISAAYSIAQTISENELTPEY
EEEECCCCCCCHHHHHHHHCCHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCE
IIPKPFDKRVLKNVAIAVAKAAIDTDVARINVDLKEIEKSLSE
ECCCCHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]