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The map label for this gene is cwlM [H]

Identifier: 167039431

GI number: 167039431

Start: 805477

End: 806187

Strand: Direct

Name: cwlM [H]

Synonym: Teth514_0776

Alternate gene names: 167039431

Gene position: 805477-806187 (Clockwise)

Preceding gene: 167039429

Following gene: 167039432

Centisome position: 32.78

GC content: 32.63

Gene sequence:

>711_bases
ATGATTATGATAGAAGAATTTACAAAATTTATTTTAATGGTGGTGAGTGAAGTGCTCATAGCTATTGATCCAGGTCACGG
AGGGAAAGATCCGGGAGCAGTGGTTGAAAACTATAAGGAAAAAGACTTAAATCTAGATATCGCTTTAAAATTAAGGGAGA
TTTTATTGGACAAAAATATAAGCGTGATTATGACAAGAGATAAAGATGAAACTGTAGATTTGCAACAAAGGTGTGATATT
GCTAATAAGAACAAAGTAGACTACTTTATTTCAATTCATTGCAATAGTTTTAAGGACCCTACTGCAAATGGTACGGAAAC
TTATGCATATCCGGGAAGCATTGTAGGCCAAAATTTAGCTCAATATGTCCAAAATGAGATAGTAGAAATGTTAAAAACGG
CGAATAGGGGGGTAAAGTATGCAACTTTTTATGTTTTAAAACACACCGTAATGCCAGCAATATTGGTTGAAACAGCTTTT
ATGTCAAATCCGCAAAATTTAGATTTGCTTTTACACAGACCAGATATTTTTGCACAGGCGATTTCAAATGGGATAATTAA
ATTTTTAGAAAGTATAAATTATCAACAAACTGATGATATTAAAAAATTGCGCAGGAAAGGGATTATAAATGAGCATTATG
ATCCTAAAAGTTTTGTCACATGGGGAGAATTTGCTTCTGTTATAATAAAAATATTGGGGGGAACAAAATGA

Upstream 100 bases:

>100_bases
GCAAAGCAAGCTCTTAGCACTTTTGAGGAATTTACCTACAAAAGGCGCATTTACATTTTTTATTTTTTATATAAACTTGT
TAATTTTCTTTGCATATTAT

Downstream 100 bases:

>100_bases
GAGATTTGATTATGACAATTTTGACTGCTGGTGTTCAACTTGGTATAATGGTCGTGCTTGGATATTTGATTGATTATTTA
AATACCAAAATTGGTATGGA

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MIMIEEFTKFILMVVSEVLIAIDPGHGGKDPGAVVENYKEKDLNLDIALKLREILLDKNISVIMTRDKDETVDLQQRCDI
ANKNKVDYFISIHCNSFKDPTANGTETYAYPGSIVGQNLAQYVQNEIVEMLKTANRGVKYATFYVLKHTVMPAILVETAF
MSNPQNLDLLLHRPDIFAQAISNGIIKFLESINYQQTDDIKKLRRKGIINEHYDPKSFVTWGEFASVIIKILGGTK

Sequences:

>Translated_236_residues
MIMIEEFTKFILMVVSEVLIAIDPGHGGKDPGAVVENYKEKDLNLDIALKLREILLDKNISVIMTRDKDETVDLQQRCDI
ANKNKVDYFISIHCNSFKDPTANGTETYAYPGSIVGQNLAQYVQNEIVEMLKTANRGVKYATFYVLKHTVMPAILVETAF
MSNPQNLDLLLHRPDIFAQAISNGIIKFLESINYQQTDDIKKLRRKGIINEHYDPKSFVTWGEFASVIIKILGGTK
>Mature_236_residues
MIMIEEFTKFILMVVSEVLIAIDPGHGGKDPGAVVENYKEKDLNLDIALKLREILLDKNISVIMTRDKDETVDLQQRCDI
ANKNKVDYFISIHCNSFKDPTANGTETYAYPGSIVGQNLAQYVQNEIVEMLKTANRGVKYATFYVLKHTVMPAILVETAF
MSNPQNLDLLLHRPDIFAQAISNGIIKFLESINYQQTDDIKKLRRKGIINEHYDPKSFVTWGEFASVIIKILGGTK

Specific function: Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable). Note=Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=233, Percent_Identity=29.1845493562232, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082163, Length=192, Percent_Identity=31.7708333333333, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790611, Length=229, Percent_Identity=25.764192139738, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26715; Mature: 26715

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMIEEFTKFILMVVSEVLIAIDPGHGGKDPGAVVENYKEKDLNLDIALKLREILLDKNI
CEEHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCC
SVIMTRDKDETVDLQQRCDIANKNKVDYFISIHCNSFKDPTANGTETYAYPGSIVGQNLA
EEEEECCCCCCCCHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEECCCHHHHHHHH
QYVQNEIVEMLKTANRGVKYATFYVLKHTVMPAILVETAFMSNPQNLDLLLHRPDIFAQA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHH
ISNGIIKFLESINYQQTDDIKKLRRKGIINEHYDPKSFVTWGEFASVIIKILGGTK
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIMIEEFTKFILMVVSEVLIAIDPGHGGKDPGAVVENYKEKDLNLDIALKLREILLDKNI
CEEHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCC
SVIMTRDKDETVDLQQRCDIANKNKVDYFISIHCNSFKDPTANGTETYAYPGSIVGQNLA
EEEEECCCCCCCCHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEECCCHHHHHHHH
QYVQNEIVEMLKTANRGVKYATFYVLKHTVMPAILVETAFMSNPQNLDLLLHRPDIFAQA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHH
ISNGIIKFLESINYQQTDDIKKLRRKGIINEHYDPKSFVTWGEFASVIIKILGGTK
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1495475 [H]