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The map label for this gene is gutA [H]

Identifier: 167039337

GI number: 167039337

Start: 712938

End: 714257

Strand: Direct

Name: gutA [H]

Synonym: Teth514_0679

Alternate gene names: 167039337

Gene position: 712938-714257 (Clockwise)

Preceding gene: 167039336

Following gene: 167039339

Centisome position: 29.01

GC content: 34.09

Gene sequence:

>1320_bases
ATGAAAAATAACAGGTTTTTTATGCTTTTAATGTATTCTTTAGGATATTTAGGGATATCAATTTTTACTCAAACGGCAGT
AAAGTGGTATCAGTATTATTACACTCCACCTGAATTTAATACACAAGGGCTTAAAATGCTTGTACCAATAAGCCTTATAG
GCTTTTCTATGATAGTTGCGAGAATATTTGACAGCATATCTGATCCAATAGTCGCTTATTATTCTGATAAATTAAATACA
AAAATGGGCAGGAGAATACCTTTTGTAGTGTTTGGTTTGCCTGTTTTGATGATTTCTTTTATAATGATGTGGTTTCCTCC
TGTTTCAGAAGAGTCAATAGTGAATTTTGTTTATCTCACTTTTGTATTAAGCTTATTTTTTGTCGCTTTCACTGTGGTAG
TGGCTCCCTATCTTGCTCTCATCCCTGAAATAACTAAAGATGCCAAAGAAAGAATTACCGTCACCATGATGCAGGGAATA
ACACAAATAATTGGAGTCATGATAGCTGAAGCAGGGTCAGGAATTTTAATTAGCATTTTTAATTTTAAAATTATGGGTAT
TATATTAGGGATTTTTGCTTTTCTTACTTTGATGCTTACGCCTATTTTTGTAAAAGAAGAAAAAATAGAGCAAGAAAATA
TACCCACGGTGGGGATGTTTGCCTCTATTAAGATGACTTTGACAAATGCAAATTTTATGTATTACCTTACTGCGGCTATA
GCTCTGTGGTTTGGGATAAATACCTTGACTATTGCTATGCCTTATATAACAGAGGTGCTTTTGAAGACCCCTGCTGAAGA
TTCTGGCTTTATGATTGCCGGTGCATTTGTGGTGGCAGTTTTGTTTAGCTTTTTTGTGCCAAAACTTACGCTCTTATACG
GGAAGAAAAAACTTCTCATGGTGTTTTCTATTATGTTTGCTGGTATTTTAGCTCTTACTGGACTTTTTGGGACTGTTTTT
AATAAGACAGTTTCTATAATTATCATTTTATTAGCTGGTATACCGCTTTCTGTATTTTTTGTAGTGCCTAATGCCATGAT
TGCCGACATAGCAGAGTTGGATGGAATTAAGACAGGGCAGAGGAGAGAGGGAATGTTTTTTGGGGCACAAGGATTTATAA
TAAAAGTAGTTATAGGAATTTCTTCTCTTGTAACACCCCTTTTATTTAAAGTATTTGGATACAGTACAGAAAATCCATTG
GGCTTACAGCTTTGTGGGCCTTTAGCAGGAATTATAATTTTGTTGAGTTTAATTGTCTTTAGCAAATACTCTCTTACCGA
AAAAGAGTTGGAAAAGTATAAGTTAGAGAGTAAAAAATAA

Upstream 100 bases:

>100_bases
GCGAGAGAAATGGCGGAGTACTATGGGGATGTGAATGAAGAGTTTGAAAGGGATTATTATGACAAGGTGTATACGAGTTT
TTTAAGAGAGGAGAAAAACC

Downstream 100 bases:

>100_bases
GGGCTTTTCGCCCTTATTTTTACTCAGATTTCCATAGACCGTGCAAATTGCAGTAGGACCATGCTTTTAAGTCTTTAGCG
TCTGTTACAAAAACCGCTTC

Product: major facilitator transporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKNNRFFMLLMYSLGYLGISIFTQTAVKWYQYYYTPPEFNTQGLKMLVPISLIGFSMIVARIFDSISDPIVAYYSDKLNT
KMGRRIPFVVFGLPVLMISFIMMWFPPVSEESIVNFVYLTFVLSLFFVAFTVVVAPYLALIPEITKDAKERITVTMMQGI
TQIIGVMIAEAGSGILISIFNFKIMGIILGIFAFLTLMLTPIFVKEEKIEQENIPTVGMFASIKMTLTNANFMYYLTAAI
ALWFGINTLTIAMPYITEVLLKTPAEDSGFMIAGAFVVAVLFSFFVPKLTLLYGKKKLLMVFSIMFAGILALTGLFGTVF
NKTVSIIIILLAGIPLSVFFVVPNAMIADIAELDGIKTGQRREGMFFGAQGFIIKVVIGISSLVTPLLFKVFGYSTENPL
GLQLCGPLAGIIILLSLIVFSKYSLTEKELEKYKLESKK

Sequences:

>Translated_439_residues
MKNNRFFMLLMYSLGYLGISIFTQTAVKWYQYYYTPPEFNTQGLKMLVPISLIGFSMIVARIFDSISDPIVAYYSDKLNT
KMGRRIPFVVFGLPVLMISFIMMWFPPVSEESIVNFVYLTFVLSLFFVAFTVVVAPYLALIPEITKDAKERITVTMMQGI
TQIIGVMIAEAGSGILISIFNFKIMGIILGIFAFLTLMLTPIFVKEEKIEQENIPTVGMFASIKMTLTNANFMYYLTAAI
ALWFGINTLTIAMPYITEVLLKTPAEDSGFMIAGAFVVAVLFSFFVPKLTLLYGKKKLLMVFSIMFAGILALTGLFGTVF
NKTVSIIIILLAGIPLSVFFVVPNAMIADIAELDGIKTGQRREGMFFGAQGFIIKVVIGISSLVTPLLFKVFGYSTENPL
GLQLCGPLAGIIILLSLIVFSKYSLTEKELEKYKLESKK
>Mature_439_residues
MKNNRFFMLLMYSLGYLGISIFTQTAVKWYQYYYTPPEFNTQGLKMLVPISLIGFSMIVARIFDSISDPIVAYYSDKLNT
KMGRRIPFVVFGLPVLMISFIMMWFPPVSEESIVNFVYLTFVLSLFFVAFTVVVAPYLALIPEITKDAKERITVTMMQGI
TQIIGVMIAEAGSGILISIFNFKIMGIILGIFAFLTLMLTPIFVKEEKIEQENIPTVGMFASIKMTLTNANFMYYLTAAI
ALWFGINTLTIAMPYITEVLLKTPAEDSGFMIAGAFVVAVLFSFFVPKLTLLYGKKKLLMVFSIMFAGILALTGLFGTVF
NKTVSIIIILLAGIPLSVFFVVPNAMIADIAELDGIKTGQRREGMFFGAQGFIIKVVIGISSLVTPLLFKVFGYSTENPL
GLQLCGPLAGIIILLSLIVFSKYSLTEKELEKYKLESKK

Specific function: Probably involved in glucitol uptake [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=459, Percent_Identity=23.3115468409586, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48929; Mature: 48929

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNRFFMLLMYSLGYLGISIFTQTAVKWYQYYYTPPEFNTQGLKMLVPISLIGFSMIVA
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHH
RIFDSISDPIVAYYSDKLNTKMGRRIPFVVFGLPVLMISFIMMWFPPVSEESIVNFVYLT
HHHHHHCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FVLSLFFVAFTVVVAPYLALIPEITKDAKERITVTMMQGITQIIGVMIAEAGSGILISIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHH
NFKIMGIILGIFAFLTLMLTPIFVKEEKIEQENIPTVGMFASIKMTLTNANFMYYLTAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHEEEEEEEECCCHHHHHHHHH
ALWFGINTLTIAMPYITEVLLKTPAEDSGFMIAGAFVVAVLFSFFVPKLTLLYGKKKLLM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VFSIMFAGILALTGLFGTVFNKTVSIIIILLAGIPLSVFFVVPNAMIADIAELDGIKTGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHCCCCCCC
RREGMFFGAQGFIIKVVIGISSLVTPLLFKVFGYSTENPLGLQLCGPLAGIIILLSLIVF
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SKYSLTEKELEKYKLESKK
HCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKNNRFFMLLMYSLGYLGISIFTQTAVKWYQYYYTPPEFNTQGLKMLVPISLIGFSMIVA
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHH
RIFDSISDPIVAYYSDKLNTKMGRRIPFVVFGLPVLMISFIMMWFPPVSEESIVNFVYLT
HHHHHHCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FVLSLFFVAFTVVVAPYLALIPEITKDAKERITVTMMQGITQIIGVMIAEAGSGILISIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHH
NFKIMGIILGIFAFLTLMLTPIFVKEEKIEQENIPTVGMFASIKMTLTNANFMYYLTAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHEEEEEEEECCCHHHHHHHHH
ALWFGINTLTIAMPYITEVLLKTPAEDSGFMIAGAFVVAVLFSFFVPKLTLLYGKKKLLM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VFSIMFAGILALTGLFGTVFNKTVSIIIILLAGIPLSVFFVVPNAMIADIAELDGIKTGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHCCCCCCC
RREGMFFGAQGFIIKVVIGISSLVTPLLFKVFGYSTENPLGLQLCGPLAGIIILLSLIVF
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SKYSLTEKELEKYKLESKK
HCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]