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The map label for this gene is fruA [H]

Identifier: 167039237

GI number: 167039237

Start: 600530

End: 601888

Strand: Direct

Name: fruA [H]

Synonym: Teth514_0578

Alternate gene names: 167039237

Gene position: 600530-601888 (Clockwise)

Preceding gene: 167039236

Following gene: 167039240

Centisome position: 24.44

GC content: 40.69

Gene sequence:

>1359_bases
ATGAAAAAGATAGTTGCTGTTACAGCCTGCCCTACAGGTATTGCCCATACCTATATGGCGGCGGAAAATCTTCAGATGGC
AGCCAAAGAAATGGGAGTAGACATAAAGGTAGAGACTCAAGGGTCAATAGGTGCAGAGAATCAACTTACTGAAGAAGATA
TAAAAGCTGCTGATGCTGTCATAATAGCAGCTGCGACAAAAGTTGATAGATCAAGATTCGCCGGTAAACCTGTACTTGAG
GTGCCAGTTGAAGATGCTATAAAAGATGCAAAAGGGCTTATTGAAAAAGCTCTTAAGATGGAAAAGCCAAAAGATTATGT
AGAAAAAGTAGAGGAGATTCATAAAGAAAGGTCAGCGCAAAGGACAGGTGCCTACAAACACCTTATGACAGGTGTTTCCT
ACATGATACCTTTTGTAGTTGCAGGTGGTATTTTAATTGCGCTTTCCTTCTTCTGGGGATATAAAGCCTTTGAGGTAGAA
AGAACTCTTCCTTGGGCTTTGATGAAAATTGGTGGCGGTTCAGCTTTTGCATTGATGGTGCCGATTTTGTCTGGCTATAT
AGCATTTTCTATTGCAGATAGGCCTGGTCTTGTTCCCGGTATGGTAGGCGGTATGCTGGCAGTATCCACTGGGGCAGGAT
TTTTAGGCGGTATCATTTCAGGATTTTTGGCAGGATATACGATAGTTTATCTGAAAAGGCTAATAAAGCTTCCTAAAACA
TTAGAGGGTTTAATGCCAATACTTATACTACCGGTATTATCAACTTTGATAGTTGGACTTTTAATGATATATGTAATAGG
TTCTCCAATGAAAGCCATAATGACATCTTTAACTGCCTGGCTTACTGGGATGAGTTCTACAAATGCAGTGATTTTTGGAG
CAATATTGGGACTCATGATGGCTTTTGATATGGGTGGTCCTGTAAACAAGACAGCTTATACTTTTGCAACTGGTCTTTTA
GCATCAAATGTTTTTGCTCCAATGGCAGCGGTCATGGCAGCTGGTATGACACCGCCTCTTGGACTTGCACTTGCAACTGT
TCTTTTTAAGAATAGATTTACAAAGGAAGAGATAGAAGCAGGTAAAGCAGCATGGGTTTTAGGTGCCTCCTTTATAACAG
AAGGAGCTATACCATTTGCGGCAGCAGACCCCTTCAGGGTAATACCTTCTATAATGGTTGGTTCTGCTGTGACGGGTGCT
CTTTCAATGCTTTTCCACATTGAATTAAGAGCTCCTCATGGAGGAATATTTGTAATACCGATAGCTGTGTCAAATCCTCT
TCTTTACATTGGAGTAATAGCAGTTGGTACAGTAGTTACTGCATTAATGGTAGCGCTTCTTAAGAAAAAAGTAGAATAA

Upstream 100 bases:

>100_bases
TAATGCATAAGGAGGTGAGAGAGGAGTTAGAAAAAGCGTCGACACCTGAGGAAGTCATAAAGGCTTTTGAGAAGTAAAAT
CTTTATGAAAGGGGATTGTT

Downstream 100 bases:

>100_bases
AAAAGGAAGGCTGTTGCCTTCCACTTTTTTTATTTGCTAAAAGGATTTTGCACCTTTACTTTGCCAGAGTACTGACCTGA
ATTTAGGTCTCTATACAAAT

Product: PTS system, fructose subfamily, IIC subunit

Products: NA

Alternate protein names: EIIBBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENQLTEEDIKAADAVIIAAATKVDRSRFAGKPVLE
VPVEDAIKDAKGLIEKALKMEKPKDYVEKVEEIHKERSAQRTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVE
RTLPWALMKIGGGSAFALMVPILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTIVYLKRLIKLPKT
LEGLMPILILPVLSTLIVGLLMIYVIGSPMKAIMTSLTAWLTGMSSTNAVIFGAILGLMMAFDMGGPVNKTAYTFATGLL
ASNVFAPMAAVMAAGMTPPLGLALATVLFKNRFTKEEIEAGKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGA
LSMLFHIELRAPHGGIFVIPIAVSNPLLYIGVIAVGTVVTALMVALLKKKVE

Sequences:

>Translated_452_residues
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENQLTEEDIKAADAVIIAAATKVDRSRFAGKPVLE
VPVEDAIKDAKGLIEKALKMEKPKDYVEKVEEIHKERSAQRTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVE
RTLPWALMKIGGGSAFALMVPILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTIVYLKRLIKLPKT
LEGLMPILILPVLSTLIVGLLMIYVIGSPMKAIMTSLTAWLTGMSSTNAVIFGAILGLMMAFDMGGPVNKTAYTFATGLL
ASNVFAPMAAVMAAGMTPPLGLALATVLFKNRFTKEEIEAGKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGA
LSMLFHIELRAPHGGIFVIPIAVSNPLLYIGVIAVGTVVTALMVALLKKKVE
>Mature_452_residues
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENQLTEEDIKAADAVIIAAATKVDRSRFAGKPVLE
VPVEDAIKDAKGLIEKALKMEKPKDYVEKVEEIHKERSAQRTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVE
RTLPWALMKIGGGSAFALMVPILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTIVYLKRLIKLPKT
LEGLMPILILPVLSTLIVGLLMIYVIGSPMKAIMTSLTAWLTGMSSTNAVIFGAILGLMMAFDMGGPVNKTAYTFATGLL
ASNVFAPMAAVMAAGMTPPLGLALATVLFKNRFTKEEIEAGKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGA
LSMLFHIELRAPHGGIFVIPIAVSNPLLYIGVIAVGTVVTALMVALLKKKVE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=450, Percent_Identity=55.7777777777778, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1786951, Length=428, Percent_Identity=42.5233644859813, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI87082348, Length=464, Percent_Identity=36.6379310344828, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790386, Length=308, Percent_Identity=46.4285714285714, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1788729, Length=382, Percent_Identity=28.2722513089005, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790387, Length=97, Percent_Identity=54.639175257732, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788730, Length=100, Percent_Identity=46, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790390, Length=79, Percent_Identity=41.7721518987342, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 47674; Mature: 47674

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENQLTEEDIKAADAV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHE
IIAAATKVDRSRFAGKPVLEVPVEDAIKDAKGLIEKALKMEKPKDYVEKVEEIHKERSAQ
EEEHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVERTLPWALMKIGGGSAFALMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCHHHHHCCCCCHHHHHH
PILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTIVYLKRLIKLPKT
HHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEGLMPILILPVLSTLIVGLLMIYVIGSPMKAIMTSLTAWLTGMSSTNAVIFGAILGLMM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AFDMGGPVNKTAYTFATGLLASNVFAPMAAVMAAGMTPPLGLALATVLFKNRFTKEEIEA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHC
GKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGALSMLFHIELRAPHGGIFVIP
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEE
IAVSNPLLYIGVIAVGTVVTALMVALLKKKVE
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENQLTEEDIKAADAV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHE
IIAAATKVDRSRFAGKPVLEVPVEDAIKDAKGLIEKALKMEKPKDYVEKVEEIHKERSAQ
EEEHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVERTLPWALMKIGGGSAFALMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCHHHHHCCCCCHHHHHH
PILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTIVYLKRLIKLPKT
HHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEGLMPILILPVLSTLIVGLLMIYVIGSPMKAIMTSLTAWLTGMSSTNAVIFGAILGLMM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AFDMGGPVNKTAYTFATGLLASNVFAPMAAVMAAGMTPPLGLALATVLFKNRFTKEEIEA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHC
GKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGALSMLFHIELRAPHGGIFVIP
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEE
IAVSNPLLYIGVIAVGTVVTALMVALLKKKVE
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254279 [H]