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The map label for this gene is thiH [H]

Identifier: 167039189

GI number: 167039189

Start: 547787

End: 549187

Strand: Direct

Name: thiH [H]

Synonym: Teth514_0530

Alternate gene names: 167039189

Gene position: 547787-549187 (Clockwise)

Preceding gene: 167039188

Following gene: 167039190

Centisome position: 22.29

GC content: 35.76

Gene sequence:

>1401_bases
ATGATAAAAGAAAAAGCAGATTTTATTGATGATGAAAAGATAAGACAGGATTTAGAAAAGGCTAAAAAAGCGACAATTAA
ATATGCATTAGAAATTATAGAGAAAGCTAAAAAGCTAAAAGGCATTACTCCTGAAGAAGCGGCGGTACTTTTAAATGTAG
AAGATGAGGATTTGCTTAATGAGATGTTTAAAGTAGCAAGGTATATAAAAGAAGAAATATATGGAAATAGAATTGTGATA
TTTGCCCCCCTTTATGTAAGTAACTACTGTGTAAACAACTGTAGATATTGTGGTTACAGACATTCTAATGAGCAGGAAAG
AAAAAAGCTTACAATGGAAGAAGTGAGAAGAGAAGTTGAGATTTTGGAAGAGATGGGACATAAGAGATTAGCAGTTGAAG
CTGGAGAAGACCCTGTAAATTGCCCTATAGATTATATTATCGATGTAATAAAGACGATATACGATACAAAACTTAAAAAC
GGAAGCATAAGAAGGGTAAATGTCAATATAGCAGCGACTACTGTGGAAAATTACAAAAAACTTAAAGAAGTAGGAATAGG
GACTTATATTTTATTCCAAGAAACTTACCATAGACCTACGTATGAATACATGCATCCACAAGGTCCAAAACACGATTACG
ACTACCATTTGACTGCTATGGATAGGGCTATGGAGGCAGGCATTGACGACGTAGGATTAGGGGTTTTGTATGGGCTTTAT
GATTACAAATACGAAACTGTTGCGATGCTTTATCATGCGAACCATTTAGAGGAGAAATTTGGAGTTGGGCCACATACTAT
TTCAGTACCGCGACTTAGACCAGCTCTTAACACTCCCATAGATAAATTCCCATATATTGTATCAGATAAAGACTTTAAAA
AATTAGTAGCCGTCATAAGAATGGCAGTGCCCTATACAGGGATGATTTTGTCTACAAGAGAGAAGCCTAAATTTAGAGAA
GAAGTAATAAGCATTGGCATTTCTCAGATTAGTGCAGGTTCTTGTACAGGAGTAGGTGGATATCATGAAGAGATATCCAA
AAAAGGTGGTTCAAAGCCACAATTTGAGGTAGAAGACAAAAGAAGTCCTAATGAAATTTTGAGGACTTTGTGTGAACAAG
GGTATCTCCCAAGTTATTGTACTGCCTGCTACAGAATGGGACGTACAGGAGACAGGTTTATGACCTTTGCGAAATCAGGG
CAAATACACAACTTCTGTCTACCTAATGCGATACTAACCTTCAAAGAGTTTTTGATTGATTACGGGGATGAAAAAACTAA
GGAAATTGGAGAAAAAGCTATAGCGGTAAATTTAGAGAAAATTCCATCAATAACTGTAAGGGAAGAGACAAAGAGAAGGC
TTACAAGAATAGAAAATGGAGAAAGAGATCTTTTCTTTTAA

Upstream 100 bases:

>100_bases
AAGAAAAATAATTTTGTTGAAAAATTAACAATAATAGTATATAATAAAAATAAAACTAAGCCCAAGGGGATTTTCCTGAG
GGTAAGGGAGGAGATAAAAT

Downstream 100 bases:

>100_bases
TATGCAAAATGCCAATAAAATAAAACTTATCAACAAACTTTCGTAAAGGAGATATTTTGCATAAGGAGCGACTTGTTACA
AAGCGGTAACAAAACTTAGC

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MIKEKADFIDDEKIRQDLEKAKKATIKYALEIIEKAKKLKGITPEEAAVLLNVEDEDLLNEMFKVARYIKEEIYGNRIVI
FAPLYVSNYCVNNCRYCGYRHSNEQERKKLTMEEVRREVEILEEMGHKRLAVEAGEDPVNCPIDYIIDVIKTIYDTKLKN
GSIRRVNVNIAATTVENYKKLKEVGIGTYILFQETYHRPTYEYMHPQGPKHDYDYHLTAMDRAMEAGIDDVGLGVLYGLY
DYKYETVAMLYHANHLEEKFGVGPHTISVPRLRPALNTPIDKFPYIVSDKDFKKLVAVIRMAVPYTGMILSTREKPKFRE
EVISIGISQISAGSCTGVGGYHEEISKKGGSKPQFEVEDKRSPNEILRTLCEQGYLPSYCTACYRMGRTGDRFMTFAKSG
QIHNFCLPNAILTFKEFLIDYGDEKTKEIGEKAIAVNLEKIPSITVREETKRRLTRIENGERDLFF

Sequences:

>Translated_466_residues
MIKEKADFIDDEKIRQDLEKAKKATIKYALEIIEKAKKLKGITPEEAAVLLNVEDEDLLNEMFKVARYIKEEIYGNRIVI
FAPLYVSNYCVNNCRYCGYRHSNEQERKKLTMEEVRREVEILEEMGHKRLAVEAGEDPVNCPIDYIIDVIKTIYDTKLKN
GSIRRVNVNIAATTVENYKKLKEVGIGTYILFQETYHRPTYEYMHPQGPKHDYDYHLTAMDRAMEAGIDDVGLGVLYGLY
DYKYETVAMLYHANHLEEKFGVGPHTISVPRLRPALNTPIDKFPYIVSDKDFKKLVAVIRMAVPYTGMILSTREKPKFRE
EVISIGISQISAGSCTGVGGYHEEISKKGGSKPQFEVEDKRSPNEILRTLCEQGYLPSYCTACYRMGRTGDRFMTFAKSG
QIHNFCLPNAILTFKEFLIDYGDEKTKEIGEKAIAVNLEKIPSITVREETKRRLTRIENGERDLFF
>Mature_466_residues
MIKEKADFIDDEKIRQDLEKAKKATIKYALEIIEKAKKLKGITPEEAAVLLNVEDEDLLNEMFKVARYIKEEIYGNRIVI
FAPLYVSNYCVNNCRYCGYRHSNEQERKKLTMEEVRREVEILEEMGHKRLAVEAGEDPVNCPIDYIIDVIKTIYDTKLKN
GSIRRVNVNIAATTVENYKKLKEVGIGTYILFQETYHRPTYEYMHPQGPKHDYDYHLTAMDRAMEAGIDDVGLGVLYGLY
DYKYETVAMLYHANHLEEKFGVGPHTISVPRLRPALNTPIDKFPYIVSDKDFKKLVAVIRMAVPYTGMILSTREKPKFRE
EVISIGISQISAGSCTGVGGYHEEISKKGGSKPQFEVEDKRSPNEILRTLCEQGYLPSYCTACYRMGRTGDRFMTFAKSG
QIHNFCLPNAILTFKEFLIDYGDEKTKEIGEKAIAVNLEKIPSITVREETKRRLTRIENGERDLFF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=365, Percent_Identity=29.5890410958904, Blast_Score=184, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53609; Mature: 53609

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEKADFIDDEKIRQDLEKAKKATIKYALEIIEKAKKLKGITPEEAAVLLNVEDEDLLN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHH
EMFKVARYIKEEIYGNRIVIFAPLYVSNYCVNNCRYCGYRHSNEQERKKLTMEEVRREVE
HHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ILEEMGHKRLAVEAGEDPVNCPIDYIIDVIKTIYDTKLKNGSIRRVNVNIAATTVENYKK
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHH
LKEVGIGTYILFQETYHRPTYEYMHPQGPKHDYDYHLTAMDRAMEAGIDDVGLGVLYGLY
HHHCCCCEEEEEEHHHCCCCHHHCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHH
DYKYETVAMLYHANHLEEKFGVGPHTISVPRLRPALNTPIDKFPYIVSDKDFKKLVAVIR
HCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHCCCHHHCCEEECCHHHHHHHHHHH
MAVPYTGMILSTREKPKFREEVISIGISQISAGSCTGVGGYHEEISKKGGSKPQFEVEDK
HHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
RSPNEILRTLCEQGYLPSYCTACYRMGRTGDRFMTFAKSGQIHNFCLPNAILTFKEFLID
CCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCEECHHHHHHHHHHHHH
YGDEKTKEIGEKAIAVNLEKIPSITVREETKRRLTRIENGERDLFF
CCCHHHHHHCCHHEEEEHHHCCCCEEHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIKEKADFIDDEKIRQDLEKAKKATIKYALEIIEKAKKLKGITPEEAAVLLNVEDEDLLN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHH
EMFKVARYIKEEIYGNRIVIFAPLYVSNYCVNNCRYCGYRHSNEQERKKLTMEEVRREVE
HHHHHHHHHHHHHCCCEEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ILEEMGHKRLAVEAGEDPVNCPIDYIIDVIKTIYDTKLKNGSIRRVNVNIAATTVENYKK
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHH
LKEVGIGTYILFQETYHRPTYEYMHPQGPKHDYDYHLTAMDRAMEAGIDDVGLGVLYGLY
HHHCCCCEEEEEEHHHCCCCHHHCCCCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHH
DYKYETVAMLYHANHLEEKFGVGPHTISVPRLRPALNTPIDKFPYIVSDKDFKKLVAVIR
HCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHCCCHHHCCEEECCHHHHHHHHHHH
MAVPYTGMILSTREKPKFREEVISIGISQISAGSCTGVGGYHEEISKKGGSKPQFEVEDK
HHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
RSPNEILRTLCEQGYLPSYCTACYRMGRTGDRFMTFAKSGQIHNFCLPNAILTFKEFLID
CCHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCCEECHHHHHHHHHHHHH
YGDEKTKEIGEKAIAVNLEKIPSITVREETKRRLTRIENGERDLFF
CCCHHHHHHCCHHEEEEHHHCCCCEEHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]