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The map label for this gene is asnO [H]

Identifier: 167039122

GI number: 167039122

Start: 474406

End: 476247

Strand: Reverse

Name: asnO [H]

Synonym: Teth514_0461

Alternate gene names: 167039122

Gene position: 476247-474406 (Counterclockwise)

Preceding gene: 167039154

Following gene: 167039118

Centisome position: 19.38

GC content: 35.23

Gene sequence:

>1842_bases
ATGTGTGGAATAACGGGATGGGTTAACTTTAATATGGACTTGTCACTTCAAAATGAAGTAATCGAAAAAATGACAGACAC
TCTTAAAAAAAGAGGTCCTGACGGCTCTGGAATATGGTTGTCAAAACATTGTGTTTTAGGCCATCGTCGATTAATTGTAA
TAGATCCACAAGGCGGTAGTCAACCAATGATAAAAAAATACGGTGAAAAAAATTTTGTCATCATCCATAATGGAGAACTC
TACAATATGGATGAATTAAAAAACGAACTCATATCCTTGGGATATACTTTTAAAACCCGTTCAGATACTGAAATTATCTT
GACATCTTATATAGAATGGGGGCCGACCTGTGTTAAAAAATTTAATGGAATATTTGCTTTTGCAATATGGGATGAAGTGC
AAAATAGGCTTTTTATTGCCAGGGACCATCTCGGAATAAAGCCACTATTTTATACCATTAAAAATGGGTCTTTGATATTT
GGTTCAGAGATTAAAGCAATATTAGCACATCCTCATGTAAAACCAGAGATAGACTCACAAGGTCTTGCAGAAATATTTGT
AATGGGACCTTCAAGGACTCCCGGATGTGGTGTCTTTAAAGACATTAAAGAATTAAAAGCCGGGCATTACCTCATATTCA
CTAAAAATGGAGCAAACATAAGCAAATACTGGTCATTACAAAGTCTTCCCCATGAAGATGACCTTGAGAAAACGGCGGAA
AAAATAAGGTATCTCCTTAAAGATTCATCAGTAAGACAACTTGTTTCAGACGTACCTTTGTGTTCATTGCTATCAGGTGG
ACTTGATTCAACTGCTGTATCTTTCTTCGCAAATGAAGAATTAAAACAAAAAGGTCATAAACTTATAACATATTCTGTAG
ATTATGTGGGTAATGATAAATATTTTACGCCAAACGAATTTCAACCAAACTCTGACGCAGACTGGGTAAAATTTGTTGCA
AATAAACTTGACACAAACCATAACTATGTATTTATAGATAATGAAGAACTTGCACATGCTCTAAAGCCAGCCTTGTACGC
TCGAGACCTTCCTGGTATGGCAGATATAGATTCATCTTTATATCTATTTTTGAAAGAAGTAAAAAAAGGAGCAACAGTCG
CTTTATCAGGCGAAGGTGCTGATGAAGTTTTTGGAGGTTATCCCTGGTTTAGAAACAAAGAAGCAATTGAAAGTCATACC
TTCCCATGGATAAGAATGGTCAATGAACGCATGAAATTGCTTTTGCCAGAAGTTGTAAAATATATAAGACCTATAGAGTA
TTTAAATGATAGATATAACGAGGCTTTAAATGAAGTGCCAAAACTTAGCGGAGAAGACCCATATAATGCTCGAATGAGGG
AAATGTTTTACCTTAACATAACAAGATTTATGCCTATGCTTCTTGATAGAATGGATAGAATGAGTATGGCAGTAGGTTTT
GAAGCAAGAGTACCTTTTTTAGACTACAGGCTTGTAGAATATGTATGGAATATACCGTGGGAAATGAAAAATTTAAATAA
CAGAGAAAAGGGAATATTGCGATACGCTCTAAAAGATTACATCCCAGAGGAAGTAATTGAAAGAAAAAAGAGTCCTTACC
CTAAAACACACAATCCAGTATTTAAAAACATCGTTAAAGAATGGCTAAAGCAAATAATTGATGACAGTACATCGCCTTTA
TTACAGCTTGTAGATAAAAAAGCAATCGTAGAACTTATAGAAACAGATGCAAAAGCCTATGACCCTGCCTGGTTTTCACA
GCTTATGGGCTCTCCACAGCTTTTCGCCTACCTCGTGCAAATAAATATGTGGCTAAAAGATTATAAAATTTCCATAGTGT
AA

Upstream 100 bases:

>100_bases
TCATGTAGAAATTAAAAGTTCATTAGAAAATTATAACATGTATCAATGGCAAAAAAAGAGAATATATTTACTTATAGATT
TAAATTTTAGGAGGATTTTT

Downstream 100 bases:

>100_bases
TAAATAAAAATACGCTAAATGGCGGGAAATCCCCGCCATTTAGTATAAGCCCGTGCTATATTTTATTTTATTTAAATTTT
CATCTTTTTTAGGCTTTCTA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MCGITGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGIWLSKHCVLGHRRLIVIDPQGGSQPMIKKYGEKNFVIIHNGEL
YNMDELKNELISLGYTFKTRSDTEIILTSYIEWGPTCVKKFNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIF
GSEIKAILAHPHVKPEIDSQGLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGANISKYWSLQSLPHEDDLEKTAE
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDKYFTPNEFQPNSDADWVKFVA
NKLDTNHNYVFIDNEELAHALKPALYARDLPGMADIDSSLYLFLKEVKKGATVALSGEGADEVFGGYPWFRNKEAIESHT
FPWIRMVNERMKLLLPEVVKYIRPIEYLNDRYNEALNEVPKLSGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGF
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKDYIPEEVIERKKSPYPKTHNPVFKNIVKEWLKQIIDDSTSPL
LQLVDKKAIVELIETDAKAYDPAWFSQLMGSPQLFAYLVQINMWLKDYKISIV

Sequences:

>Translated_613_residues
MCGITGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGIWLSKHCVLGHRRLIVIDPQGGSQPMIKKYGEKNFVIIHNGEL
YNMDELKNELISLGYTFKTRSDTEIILTSYIEWGPTCVKKFNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIF
GSEIKAILAHPHVKPEIDSQGLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGANISKYWSLQSLPHEDDLEKTAE
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDKYFTPNEFQPNSDADWVKFVA
NKLDTNHNYVFIDNEELAHALKPALYARDLPGMADIDSSLYLFLKEVKKGATVALSGEGADEVFGGYPWFRNKEAIESHT
FPWIRMVNERMKLLLPEVVKYIRPIEYLNDRYNEALNEVPKLSGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGF
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKDYIPEEVIERKKSPYPKTHNPVFKNIVKEWLKQIIDDSTSPL
LQLVDKKAIVELIETDAKAYDPAWFSQLMGSPQLFAYLVQINMWLKDYKISIV
>Mature_613_residues
MCGITGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGIWLSKHCVLGHRRLIVIDPQGGSQPMIKKYGEKNFVIIHNGEL
YNMDELKNELISLGYTFKTRSDTEIILTSYIEWGPTCVKKFNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIF
GSEIKAILAHPHVKPEIDSQGLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGANISKYWSLQSLPHEDDLEKTAE
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDKYFTPNEFQPNSDADWVKFVA
NKLDTNHNYVFIDNEELAHALKPALYARDLPGMADIDSSLYLFLKEVKKGATVALSGEGADEVFGGYPWFRNKEAIESHT
FPWIRMVNERMKLLLPEVVKYIRPIEYLNDRYNEALNEVPKLSGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGF
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKDYIPEEVIERKKSPYPKTHNPVFKNIVKEWLKQIIDDSTSPL
LQLVDKKAIVELIETDAKAYDPAWFSQLMGSPQLFAYLVQINMWLKDYKISIV

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=414, Percent_Identity=27.0531400966184, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI168229252, Length=414, Percent_Identity=27.0531400966184, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI168229250, Length=414, Percent_Identity=27.0531400966184, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI296010848, Length=387, Percent_Identity=26.6149870801034, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI296010852, Length=316, Percent_Identity=25.6329113924051, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI296010850, Length=316, Percent_Identity=25.6329113924051, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786889, Length=575, Percent_Identity=28.5217391304348, Blast_Score=183, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71993933, Length=557, Percent_Identity=26.3913824057451, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25147557, Length=557, Percent_Identity=26.3913824057451, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17560178, Length=573, Percent_Identity=25.4799301919721, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI25147560, Length=281, Percent_Identity=28.8256227758007, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325403, Length=598, Percent_Identity=24.5819397993311, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321563, Length=576, Percent_Identity=25, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45553209, Length=537, Percent_Identity=25.512104283054, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70606; Mature: 70606

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGIWLSKHCVLGHRRLIVIDPQGGS
CCCCEEEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCEEEEECCCCCC
QPMIKKYGEKNFVIIHNGELYNMDELKNELISLGYTFKTRSDTEIILTSYIEWGPTCVKK
CHHHHHCCCCCEEEEECCCEECHHHHHHHHHHCCCEEECCCCCEEEEEHHHHCCHHHHHH
FNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIFGSEIKAILAHPHVKPEIDSQ
CCCEEEEEEHHHHHCCEEEEECCCCCCEEEEEEECCCEEECCHHHHHHHCCCCCCCCCCC
GLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGANISKYWSLQSLPHEDDLEKTAE
CCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCCCCHHHHHHH
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDK
HHHHHHCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCC
YFTPNEFQPNSDADWVKFVANKLDTNHNYVFIDNEELAHALKPALYARDLPGMADIDSSL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHH
YLFLKEVKKGATVALSGEGADEVFGGYPWFRNKEAIESHTFPWIRMVNERMKLLLPEVVK
HHHHHHHCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
YIRPIEYLNDRYNEALNEVPKLSGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKDYIPEEVIERKKSPYPKTHNPV
CCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHH
FKNIVKEWLKQIIDDSTSPLLQLVDKKAIVELIETDAKAYDPAWFSQLMGSPQLFAYLVQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHH
INMWLKDYKISIV
HHHHHCCEEEEEC
>Mature Secondary Structure
MCGITGWVNFNMDLSLQNEVIEKMTDTLKKRGPDGSGIWLSKHCVLGHRRLIVIDPQGGS
CCCCEEEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCEEEEECCCCCC
QPMIKKYGEKNFVIIHNGELYNMDELKNELISLGYTFKTRSDTEIILTSYIEWGPTCVKK
CHHHHHCCCCCEEEEECCCEECHHHHHHHHHHCCCEEECCCCCEEEEEHHHHCCHHHHHH
FNGIFAFAIWDEVQNRLFIARDHLGIKPLFYTIKNGSLIFGSEIKAILAHPHVKPEIDSQ
CCCEEEEEEHHHHHCCEEEEECCCCCCEEEEEEECCCEEECCHHHHHHHCCCCCCCCCCC
GLAEIFVMGPSRTPGCGVFKDIKELKAGHYLIFTKNGANISKYWSLQSLPHEDDLEKTAE
CCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCCCCHHHHHHH
KIRYLLKDSSVRQLVSDVPLCSLLSGGLDSTAVSFFANEELKQKGHKLITYSVDYVGNDK
HHHHHHCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCC
YFTPNEFQPNSDADWVKFVANKLDTNHNYVFIDNEELAHALKPALYARDLPGMADIDSSL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCHHH
YLFLKEVKKGATVALSGEGADEVFGGYPWFRNKEAIESHTFPWIRMVNERMKLLLPEVVK
HHHHHHHCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
YIRPIEYLNDRYNEALNEVPKLSGEDPYNARMREMFYLNITRFMPMLLDRMDRMSMAVGF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EARVPFLDYRLVEYVWNIPWEMKNLNNREKGILRYALKDYIPEEVIERKKSPYPKTHNPV
CCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHH
FKNIVKEWLKQIIDDSTSPLLQLVDKKAIVELIETDAKAYDPAWFSQLMGSPQLFAYLVQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHH
INMWLKDYKISIV
HHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]