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The map label for this gene is aglA [H]

Identifier: 167039057

GI number: 167039057

Start: 403081

End: 404523

Strand: Reverse

Name: aglA [H]

Synonym: Teth514_0394

Alternate gene names: 167039057

Gene position: 404523-403081 (Counterclockwise)

Preceding gene: 167039058

Following gene: 167039051

Centisome position: 16.46

GC content: 35.48

Gene sequence:

>1443_bases
ATGCCATCAGTAAAAATTGGTATTATAGGTGCAGGAAGTGCTGTATTTTCTCTGAGGTTGGTGAGCGATTTATGCAAAAC
TCCAGCGTTATATGGAAGCTTAGTAACTCTCATGGATATCGATGAAGAAAGATTAGAGGCCGTGTACATACTTGCAAAAA
GGTATGTGGAAGAAGTAGGAGCAGAGTTAAAGTTTGAAAAAACTAAAAATTTAGAAGATGCCATAATAGATGCAGATTTC
GTAATAAATACAGCAATGGTAGGAGGACATACTTATCTAGAAAAAGTCAGACAGATTAGCGAAAAATATGGCTATTATAG
AGGAATAGATGCACAGGAATTTAATATGGTTTCAGATTATTACACTTTCTCAAATTACAATCAGTTGAAATATTTCGTAG
AAATTGCCAAGAAAATTGAAAAACTGTCTCCAAACGCCTGGTATTTACAAGCTGCTAATCCCGTATTTGAAGGCACAACT
CTTGTAACAAGGACCTCTTCGATAAAAGCAGTTGGATTCTGCCATGGACATCTTGCATTAAAAGAAGTATTTGACACACT
GGGACTTAAGCATAATAAAGTAGATTGGCAAGTAGCAGGAGTAAATCACGGAATATGGCTTAATAGATTTATATATGAAG
GAAAAAGTGCTTACGAAAAGCTCAACACCTGGATAGAGGAAAATTCTCACAACTGGAAACCTCTTCATCCATTTAATGAC
CAGCTATCCTCAGCTGCCATTGATATGTACAAGTTTCACGGAGTTTTGCCTGTTGGCGATACCGTAAGAAATGCTTCTTG
GCGGTATCATAAAAACCTGGAAACAAAGAAAAAGTGGTATGGAGAACCTTGGTGTGGTGCAGACTCTGAAATAGGTTGGA
AATGGTACCAAGAAACATTAGGAAAAATTACAGACATCACTAAAAAGATTGCAAAGTTCTTGATAGAAAATCCAAAAGCA
AAGTTTAGCGATATAAAAGAAATTTTCGGTCAAGAGGCAAAAGACAATGAATTACTACAGGAAATGGAAAAAATACTAGA
CCCAGAGCAAAAAAGTGAAGAACAGCACATTCCTTTCGTAGAATCGATTGTAACCGGCAAAAAAGAAAGATTTGTAGTAA
ATATACCAAATAGAAGAATAATTCCTGCAGTAGAAAATGATGTTGTTGTAGAAGTACCTGCAATAGTAGATAGCGAAGGA
ATACATCCAGAAAAAATAGAACCCATGCTCCCAGAGAGAGTAATAAAGTATTACCTAAAACCGAGAATTATGAGAATGGA
AATGGCAGTAGAAGCATTTTTAACAGGGGACATAGACATAATAAAAGAACTTCTGTACAGAGACCCTAGAACTCAAAATG
ATGGGCAGGTAGAAAAAGTTTTAGAAGAAATTCTATCCCTACCAGAAAATGAAGAGATGAAAAAACATTATTTAAAAAAA
TAG

Upstream 100 bases:

>100_bases
CCTCTAATTATAATGGTACTTATCTTCCAAAAAAGGATAATCTCCGGTTTGACTGCTGGAGCAGTAAAAGGATAAAAATT
TTATGAAAGGAGTGTTTTAA

Downstream 100 bases:

>100_bases
TGAGGCTAAATTTAACTAATGAGGTGCCATTTGTAAAATCCTTTGTACAAATGGCACCTTTTTTATTTTACTCTCTTTTT
AGCACCTTCTTTTTTATTTC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Maltase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPSVKIGIIGAGSAVFSLRLVSDLCKTPALYGSLVTLMDIDEERLEAVYILAKRYVEEVGAELKFEKTKNLEDAIIDADF
VINTAMVGGHTYLEKVRQISEKYGYYRGIDAQEFNMVSDYYTFSNYNQLKYFVEIAKKIEKLSPNAWYLQAANPVFEGTT
LVTRTSSIKAVGFCHGHLALKEVFDTLGLKHNKVDWQVAGVNHGIWLNRFIYEGKSAYEKLNTWIEENSHNWKPLHPFND
QLSSAAIDMYKFHGVLPVGDTVRNASWRYHKNLETKKKWYGEPWCGADSEIGWKWYQETLGKITDITKKIAKFLIENPKA
KFSDIKEIFGQEAKDNELLQEMEKILDPEQKSEEQHIPFVESIVTGKKERFVVNIPNRRIIPAVENDVVVEVPAIVDSEG
IHPEKIEPMLPERVIKYYLKPRIMRMEMAVEAFLTGDIDIIKELLYRDPRTQNDGQVEKVLEEILSLPENEEMKKHYLKK

Sequences:

>Translated_480_residues
MPSVKIGIIGAGSAVFSLRLVSDLCKTPALYGSLVTLMDIDEERLEAVYILAKRYVEEVGAELKFEKTKNLEDAIIDADF
VINTAMVGGHTYLEKVRQISEKYGYYRGIDAQEFNMVSDYYTFSNYNQLKYFVEIAKKIEKLSPNAWYLQAANPVFEGTT
LVTRTSSIKAVGFCHGHLALKEVFDTLGLKHNKVDWQVAGVNHGIWLNRFIYEGKSAYEKLNTWIEENSHNWKPLHPFND
QLSSAAIDMYKFHGVLPVGDTVRNASWRYHKNLETKKKWYGEPWCGADSEIGWKWYQETLGKITDITKKIAKFLIENPKA
KFSDIKEIFGQEAKDNELLQEMEKILDPEQKSEEQHIPFVESIVTGKKERFVVNIPNRRIIPAVENDVVVEVPAIVDSEG
IHPEKIEPMLPERVIKYYLKPRIMRMEMAVEAFLTGDIDIIKELLYRDPRTQNDGQVEKVLEEILSLPENEEMKKHYLKK
>Mature_479_residues
PSVKIGIIGAGSAVFSLRLVSDLCKTPALYGSLVTLMDIDEERLEAVYILAKRYVEEVGAELKFEKTKNLEDAIIDADFV
INTAMVGGHTYLEKVRQISEKYGYYRGIDAQEFNMVSDYYTFSNYNQLKYFVEIAKKIEKLSPNAWYLQAANPVFEGTTL
VTRTSSIKAVGFCHGHLALKEVFDTLGLKHNKVDWQVAGVNHGIWLNRFIYEGKSAYEKLNTWIEENSHNWKPLHPFNDQ
LSSAAIDMYKFHGVLPVGDTVRNASWRYHKNLETKKKWYGEPWCGADSEIGWKWYQETLGKITDITKKIAKFLIENPKAK
FSDIKEIFGQEAKDNELLQEMEKILDPEQKSEEQHIPFVESIVTGKKERFVVNIPNRRIIPAVENDVVVEVPAIVDSEGI
HPEKIEPMLPERVIKYYLKPRIMRMEMAVEAFLTGDIDIIKELLYRDPRTQNDGQVEKVLEEILSLPENEEMKKHYLKK

Specific function: Alpha-glycosidase with a very broad specificity. Hydrolyzes maltose and other small maltooligosaccharides but is inactive against the polymeric substrate starch. AglA is not specific with respect to the configuration at the C-4 position of its substrates

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=205, Percent_Identity=26.3414634146341, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788029, Length=223, Percent_Identity=26.9058295964126, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 55325; Mature: 55194

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVKIGIIGAGSAVFSLRLVSDLCKTPALYGSLVTLMDIDEERLEAVYILAKRYVEEVG
CCCEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHC
AELKFEKTKNLEDAIIDADFVINTAMVGGHTYLEKVRQISEKYGYYRGIDAQEFNMVSDY
CCEEEHHHCCHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
YTFSNYNQLKYFVEIAKKIEKLSPNAWYLQAANPVFEGTTLVTRTSSIKAVGFCHGHLAL
HHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEHHHHHH
KEVFDTLGLKHNKVDWQVAGVNHGIWLNRFIYEGKSAYEKLNTWIEENSHNWKPLHPFND
HHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHH
QLSSAAIDMYKFHGVLPVGDTVRNASWRYHKNLETKKKWYGEPWCGADSEIGWKWYQETL
HHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHH
GKITDITKKIAKFLIENPKAKFSDIKEIFGQEAKDNELLQEMEKILDPEQKSEEQHIPFV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHH
ESIVTGKKERFVVNIPNRRIIPAVENDVVVEVPAIVDSEGIHPEKIEPMLPERVIKYYLK
HHHHCCCCCEEEEECCCCEECCCCCCCEEEEECCEECCCCCCHHHCCCCCHHHHHHHHHC
PRIMRMEMAVEAFLTGDIDIIKELLYRDPRTQNDGQVEKVLEEILSLPENEEMKKHYLKK
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure 
PSVKIGIIGAGSAVFSLRLVSDLCKTPALYGSLVTLMDIDEERLEAVYILAKRYVEEVG
CCEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHC
AELKFEKTKNLEDAIIDADFVINTAMVGGHTYLEKVRQISEKYGYYRGIDAQEFNMVSDY
CCEEEHHHCCHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
YTFSNYNQLKYFVEIAKKIEKLSPNAWYLQAANPVFEGTTLVTRTSSIKAVGFCHGHLAL
HHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEHHHHHH
KEVFDTLGLKHNKVDWQVAGVNHGIWLNRFIYEGKSAYEKLNTWIEENSHNWKPLHPFND
HHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHH
QLSSAAIDMYKFHGVLPVGDTVRNASWRYHKNLETKKKWYGEPWCGADSEIGWKWYQETL
HHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHH
GKITDITKKIAKFLIENPKAKFSDIKEIFGQEAKDNELLQEMEKILDPEQKSEEQHIPFV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHH
ESIVTGKKERFVVNIPNRRIIPAVENDVVVEVPAIVDSEGIHPEKIEPMLPERVIKYYLK
HHHHCCCCCEEEEECCCCEECCCCCCCEEEEECCEECCCCCCHHHCCCCCHHHHHHHHHC
PRIMRMEMAVEAFLTGDIDIIKELLYRDPRTQNDGQVEKVLEEILSLPENEEMKKHYLKK
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571; 10972187; 12062450; 12588867 [H]