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The map label for this gene is ltaA [H]

Identifier: 167038875

GI number: 167038875

Start: 217106

End: 218143

Strand: Reverse

Name: ltaA [H]

Synonym: Teth514_0206

Alternate gene names: 167038875

Gene position: 218143-217106 (Counterclockwise)

Preceding gene: 167038876

Following gene: 167038863

Centisome position: 8.88

GC content: 38.15

Gene sequence:

>1038_bases
ATGAAATATATAGATTTGAGAAGCGATACAGTAACTCTTCCAACACAAGAAATGCGAGAAGCTATGTATAAAGCTGAGGT
AGGTGATGACGTCTACGGCGAAGAACCTACAGTGAGAAAACTTGAAGAAATGGCAGCAGAAATGTTGGGAAAAGAAGCAG
CAATGCTAGTAACAAGCGGTACACAAGGTAACCAAGTCTCAATAATGACTCACACTCATCCAGGAGAAGAAATTATTGTA
GAAGAAAACTGCCATATCATAACTTATGAAGTTGGAGGAGTGGGATACCTTGCAGGCGTACAGACAAAAGCTTTGAAAAG
CAACAAAGGTGTCTTAAATCCTGCCGATGTAGAAAAGGCCATAAGGCCAAAGGACATACATTACCCCGTCACAAGTCTTA
TCTGCCTTGAAAATACTCATAACAGAGCTGGTGGCACTGTAACTCCAATTGAAATTATGAAGGAAATATACGAAATAGCT
CAAAAACATAATATACCTGTTCATTTAGATGGTGCGAGAATATTCAATGCCGCTACTTATCTTAAAGTAGATGTAAGAGA
AATAGCCAAATATGCAGATAGTGTAATGTTTTGCCTCTCCAAAGGCTTATGCGCCCCTATTGGGTCGGTAGTCGTCGGCA
CAAAAGATTTCATTGAAAAAGCAAGAAAATACCGCAAAATGTTAGGTGGTGGAATGAGACAAGCAGGTTTTATTGCAGCT
GCTGGCATTGTAGCACTTGAAAAAATGACAAAAAGGCTACAGGAAGACCATGACAATGCAAGATTTTTAGCAGAAGGATT
AAAAAATATACCGGGAATAAACCTCGACCTGGAAACAGTGCAGACTAATATTGTAATGACAGACATTTCAGGCACAGGAA
TGACAGGTAAGGAACTATCCTTAAGACTAAAAGAGCATGGAATTTTAATAAACGGCGGGAATGATTTTGCAGTAAGATTT
GTAACTCATTATTACATAAGTAAAAAAGAGATTGAAAAAACTCTCGATGCAATTGAGAAAATTGTAAGACAGTTTTAG

Upstream 100 bases:

>100_bases
CTCTTTAACAAAGTAGGTCGTCGCTTTAGTTTGCTGATAAATGAAATCTTTATGAGATGTGATATAATGGAAGTAAGCTT
AAGTTGATAGGGGGATAAAA

Downstream 100 bases:

>100_bases
AGAGAAGGTATCCCCTTCTCTCTATGTATATTCTATTCTCACCTTACTCCCTTCGCCAGAGACGGCAGGTGGTGTAACAA
TTAGTTTTACAGGTACTCTG

Product: aromatic amino acid beta-eliminating lyase/threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKYIDLRSDTVTLPTQEMREAMYKAEVGDDVYGEEPTVRKLEEMAAEMLGKEAAMLVTSGTQGNQVSIMTHTHPGEEIIV
EENCHIITYEVGGVGYLAGVQTKALKSNKGVLNPADVEKAIRPKDIHYPVTSLICLENTHNRAGGTVTPIEIMKEIYEIA
QKHNIPVHLDGARIFNAATYLKVDVREIAKYADSVMFCLSKGLCAPIGSVVVGTKDFIEKARKYRKMLGGGMRQAGFIAA
AGIVALEKMTKRLQEDHDNARFLAEGLKNIPGINLDLETVQTNIVMTDISGTGMTGKELSLRLKEHGILINGGNDFAVRF
VTHYYISKKEIEKTLDAIEKIVRQF

Sequences:

>Translated_345_residues
MKYIDLRSDTVTLPTQEMREAMYKAEVGDDVYGEEPTVRKLEEMAAEMLGKEAAMLVTSGTQGNQVSIMTHTHPGEEIIV
EENCHIITYEVGGVGYLAGVQTKALKSNKGVLNPADVEKAIRPKDIHYPVTSLICLENTHNRAGGTVTPIEIMKEIYEIA
QKHNIPVHLDGARIFNAATYLKVDVREIAKYADSVMFCLSKGLCAPIGSVVVGTKDFIEKARKYRKMLGGGMRQAGFIAA
AGIVALEKMTKRLQEDHDNARFLAEGLKNIPGINLDLETVQTNIVMTDISGTGMTGKELSLRLKEHGILINGGNDFAVRF
VTHYYISKKEIEKTLDAIEKIVRQF
>Mature_345_residues
MKYIDLRSDTVTLPTQEMREAMYKAEVGDDVYGEEPTVRKLEEMAAEMLGKEAAMLVTSGTQGNQVSIMTHTHPGEEIIV
EENCHIITYEVGGVGYLAGVQTKALKSNKGVLNPADVEKAIRPKDIHYPVTSLICLENTHNRAGGTVTPIEIMKEIYEIA
QKHNIPVHLDGARIFNAATYLKVDVREIAKYADSVMFCLSKGLCAPIGSVVVGTKDFIEKARKYRKMLGGGMRQAGFIAA
AGIVALEKMTKRLQEDHDNARFLAEGLKNIPGINLDLETVQTNIVMTDISGTGMTGKELSLRLKEHGILINGGNDFAVRF
VTHYYISKKEIEKTLDAIEKIVRQF

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=329, Percent_Identity=44.3768996960486, Blast_Score=279, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI193206522, Length=348, Percent_Identity=42.816091954023, Blast_Score=277, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI193206520, Length=348, Percent_Identity=42.816091954023, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI193206524, Length=348, Percent_Identity=42.816091954023, Blast_Score=276, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6320789, Length=348, Percent_Identity=36.4942528735632, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI21355439, Length=370, Percent_Identity=41.0810810810811, Blast_Score=276, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 38115; Mature: 38115

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIDLRSDTVTLPTQEMREAMYKAEVGDDVYGEEPTVRKLEEMAAEMLGKEAAMLVTSG
CCEEECCCCEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
TQGNQVSIMTHTHPGEEIIVEENCHIITYEVGGVGYLAGVQTKALKSNKGVLNPADVEKA
CCCCEEEEEEECCCCCEEEEECCCEEEEEECCCEEEECCCHHHHHHCCCCCCCHHHHHHC
IRPKDIHYPVTSLICLENTHNRAGGTVTPIEIMKEIYEIAQKHNIPVHLDGARIFNAATY
CCCCCCCCCHHHEEEEECCCCCCCCEECHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHE
LKVDVREIAKYADSVMFCLSKGLCAPIGSVVVGTKDFIEKARKYRKMLGGGMRQAGFIAA
EEEHHHHHHHHHHHHHHHHHCCCCCCCCHHEECCHHHHHHHHHHHHHHCCCCCHHHHHHH
AGIVALEKMTKRLQEDHDNARFLAEGLKNIPGINLDLETVQTNIVMTDISGTGMTGKELS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEHEEEEEEEEEEECCCCCCCCHHHE
LRLKEHGILINGGNDFAVRFVTHYYISKKEIEKTLDAIEKIVRQF
EEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYIDLRSDTVTLPTQEMREAMYKAEVGDDVYGEEPTVRKLEEMAAEMLGKEAAMLVTSG
CCEEECCCCEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
TQGNQVSIMTHTHPGEEIIVEENCHIITYEVGGVGYLAGVQTKALKSNKGVLNPADVEKA
CCCCEEEEEEECCCCCEEEEECCCEEEEEECCCEEEECCCHHHHHHCCCCCCCHHHHHHC
IRPKDIHYPVTSLICLENTHNRAGGTVTPIEIMKEIYEIAQKHNIPVHLDGARIFNAATY
CCCCCCCCCHHHEEEEECCCCCCCCEECHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHE
LKVDVREIAKYADSVMFCLSKGLCAPIGSVVVGTKDFIEKARKYRKMLGGGMRQAGFIAA
EEEHHHHHHHHHHHHHHHHHCCCCCCCCHHEECCHHHHHHHHHHHHHHCCCCCHHHHHHH
AGIVALEKMTKRLQEDHDNARFLAEGLKNIPGINLDLETVQTNIVMTDISGTGMTGKELS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEHEEEEEEEEEEECCCCCCCCHHHE
LRLKEHGILINGGNDFAVRFVTHYYISKKEIEKTLDAIEKIVRQF
EEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]