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The map label for this gene is nfsA [C]

Identifier: 167038816

GI number: 167038816

Start: 141317

End: 141871

Strand: Direct

Name: nfsA [C]

Synonym: Teth514_0144

Alternate gene names: 167038816

Gene position: 141317-141871 (Clockwise)

Preceding gene: 167038815

Following gene: 167038819

Centisome position: 5.75

GC content: 45.41

Gene sequence:

>555_bases
GTGAACGAAGTTATCAACGCCATTAAATCCCGAAGGAGTATAAGGCGGTTTAAGCCCGAACAGGTAAGGGATGAAGAAAT
AGAGGCCATTATCGAAGCAGCAATCCATGCTCCCAGCGGCCACAACGAGCAGCCTTGGCATTTTACCGTCATTCAGAATA
AAGATCTCATCGACCATTTCAGCAATGTCACAAAAAAAGCCATGGCCGAATCTAAAGTCCCATGGATAGCCGCACTAGGC
AAAAATGAGAAATTGCATCTTTTTTATAACGCACCGACCGTAATAGTAGTATCGGGCAAAAAAGGTTCTTATTCACCTCT
TATCGATTGCAGCGCCGCCACTCAAAACATGCTGCTTGCAGCTCACAGCCTGGGACTCGGAGCGTGCTGGATCGGCTTAA
TAAATTTGATATTTAAGATGGAAGAAGAGGTGAAAAAACTCTCGCTGCCACACGGCTATGAGCCTTACTTTGCAGTGGCC
TTGGGTTACCCAGACCTGGAAAAACCACCGCGTCCCATAGAAAGGAAAAAGGATGTAGTAAATTACATAAAGTGA

Upstream 100 bases:

>100_bases
GTCAGATTGAAAAGTCACGCAAATATACTTTAGAAGACTTGAAGCGAAAGCTTCAAATTTTTAAAGTCGATCCATAAAAG
GTAGGGAGGAGGAAAATATT

Downstream 100 bases:

>100_bases
TAAAAAATCCACCCCCAAACTTTGACAGTTTGAGGGTGGATTTTTTGCTTAATAATTTTCGTACATATCTTCCAATATCT
TCAAAATTTCTTCTCTTTCC

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MNEVINAIKSRRSIRRFKPEQVRDEEIEAIIEAAIHAPSGHNEQPWHFTVIQNKDLIDHFSNVTKKAMAESKVPWIAALG
KNEKLHLFYNAPTVIVVSGKKGSYSPLIDCSAATQNMLLAAHSLGLGACWIGLINLIFKMEEEVKKLSLPHGYEPYFAVA
LGYPDLEKPPRPIERKKDVVNYIK

Sequences:

>Translated_184_residues
MNEVINAIKSRRSIRRFKPEQVRDEEIEAIIEAAIHAPSGHNEQPWHFTVIQNKDLIDHFSNVTKKAMAESKVPWIAALG
KNEKLHLFYNAPTVIVVSGKKGSYSPLIDCSAATQNMLLAAHSLGLGACWIGLINLIFKMEEEVKKLSLPHGYEPYFAVA
LGYPDLEKPPRPIERKKDVVNYIK
>Mature_184_residues
MNEVINAIKSRRSIRRFKPEQVRDEEIEAIIEAAIHAPSGHNEQPWHFTVIQNKDLIDHFSNVTKKAMAESKVPWIAALG
KNEKLHLFYNAPTVIVVSGKKGSYSPLIDCSAATQNMLLAAHSLGLGACWIGLINLIFKMEEEVKKLSLPHGYEPYFAVA
LGYPDLEKPPRPIERKKDVVNYIK

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=166, Percent_Identity=30.7228915662651, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 20743; Mature: 20743

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVINAIKSRRSIRRFKPEQVRDEEIEAIIEAAIHAPSGHNEQPWHFTVIQNKDLIDHF
CHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHH
SNVTKKAMAESKVPWIAALGKNEKLHLFYNAPTVIVVSGKKGSYSPLIDCSAATQNMLLA
HHHHHHHHHHCCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCEEECHHHHHHHHHH
AHSLGLGACWIGLINLIFKMEEEVKKLSLPHGYEPYFAVALGYPDLEKPPRPIERKKDVV
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
NYIK
HHCC
>Mature Secondary Structure
MNEVINAIKSRRSIRRFKPEQVRDEEIEAIIEAAIHAPSGHNEQPWHFTVIQNKDLIDHF
CHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHH
SNVTKKAMAESKVPWIAALGKNEKLHLFYNAPTVIVVSGKKGSYSPLIDCSAATQNMLLA
HHHHHHHHHHCCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCEEECHHHHHHHHHH
AHSLGLGACWIGLINLIFKMEEEVKKLSLPHGYEPYFAVALGYPDLEKPPRPIERKKDVV
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
NYIK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]