Click here to switch to the map view.

The map label for this gene is levE [H]

Identifier: 167038808

GI number: 167038808

Start: 132737

End: 133237

Strand: Direct

Name: levE [H]

Synonym: Teth514_0135

Alternate gene names: 167038808

Gene position: 132737-133237 (Clockwise)

Preceding gene: 167038807

Following gene: 167038809

Centisome position: 5.4

GC content: 30.94

Gene sequence:

>501_bases
ATGGGAGAAATAGTGTTTGCTAGAGTAGATGACAGATTAATTCATGGTCAAGTAATGACCAAATGGGTAAAAGGATATAA
TTGTAATTCTATTTTTATAATTGATAATTCCCTTGCGAATGATGAGTTTATGAAACAAGTTTATATGATGTCTGCCTCAG
CAGCTCATGTAGCACTGAAAATACTTAGTACAGAAGAAACGATAACATTATGGAAAAAAGATAAGTTTAACGGATTTAGA
GTAATTTTACTTTATAAGGATATAATTTCGGTTAAAGAAACAATTGCCAATGGCTTGCCAATAAAAAAATTGAATATAGG
AGGTATTGCTAAAAGCCAAGATAGGAAATTTATAATACCCAGTGTTAGCTTGAAAAAAGAAGAGTTAGAAATACTTGTGG
AACTTGCGGATAATTTTGGCGTGGAAGTCTTTTTTCAAACAGTTCCAGATAGTAAAAGAGTTGATTTAGAGGATGCCCTA
AAGTTATTTGGTTTAAAATAA

Upstream 100 bases:

>100_bases
TTTAAATATATGTTATTGATCCTTGCAGAACCCTTAAAATTCATGTATCTTTGGGTTCTGCAAGGAATTTTGAGATAAAA
AAGAAAGGTGAGTGATTAAA

Downstream 100 bases:

>100_bases
ATAAACACTACATTTTACTATTATAGACATGAACAAAATGTTAAACCAAGGTCATTAAAAATAAAATTCATAGAACACAT
AGATAGGTTTCTTTCTTTAA

Product: PTS system sorbose subfamily IIB component

Products: NA

Alternate protein names: EIIB-Fru; PTS system fructose-specific EIIB component; p18 [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MGEIVFARVDDRLIHGQVMTKWVKGYNCNSIFIIDNSLANDEFMKQVYMMSASAAHVALKILSTEETITLWKKDKFNGFR
VILLYKDIISVKETIANGLPIKKLNIGGIAKSQDRKFIIPSVSLKKEELEILVELADNFGVEVFFQTVPDSKRVDLEDAL
KLFGLK

Sequences:

>Translated_166_residues
MGEIVFARVDDRLIHGQVMTKWVKGYNCNSIFIIDNSLANDEFMKQVYMMSASAAHVALKILSTEETITLWKKDKFNGFR
VILLYKDIISVKETIANGLPIKKLNIGGIAKSQDRKFIIPSVSLKKEELEILVELADNFGVEVFFQTVPDSKRVDLEDAL
KLFGLK
>Mature_165_residues
GEIVFARVDDRLIHGQVMTKWVKGYNCNSIFIIDNSLANDEFMKQVYMMSASAAHVALKILSTEETITLWKKDKFNGFRV
ILLYKDIISVKETIANGLPIKKLNIGGIAKSQDRKFIIPSVSLKKEELEILVELADNFGVEVFFQTVPDSKRVDLEDALK
LFGLK

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator [H]

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=156, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI87082217, Length=156, Percent_Identity=26.9230769230769, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18813; Mature: 18682

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEIVFARVDDRLIHGQVMTKWVKGYNCNSIFIIDNSLANDEFMKQVYMMSASAAHVALK
CCCEEEEECCCEEHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHEEEEE
ILSTEETITLWKKDKFNGFRVILLYKDIISVKETIANGLPIKKLNIGGIAKSQDRKFIIP
EECCCCEEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEC
SVSLKKEELEILVELADNFGVEVFFQTVPDSKRVDLEDALKLFGLK
CCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
GEIVFARVDDRLIHGQVMTKWVKGYNCNSIFIIDNSLANDEFMKQVYMMSASAAHVALK
CCEEEEECCCEEHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHEEEEE
ILSTEETITLWKKDKFNGFRVILLYKDIISVKETIANGLPIKKLNIGGIAKSQDRKFIIP
EECCCCEEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEC
SVSLKKEELEILVELADNFGVEVFFQTVPDSKRVDLEDALKLFGLK
CCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377; 9551099 [H]