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The map label for this gene is levD [H]

Identifier: 167038805

GI number: 167038805

Start: 130293

End: 130703

Strand: Direct

Name: levD [H]

Synonym: Teth514_0132

Alternate gene names: 167038805

Gene position: 130293-130703 (Clockwise)

Preceding gene: 167038804

Following gene: 167038806

Centisome position: 5.3

GC content: 32.12

Gene sequence:

>411_bases
GTGAAAGAAAAATTCGTACTAATTATTACACATGGAAATTTTGGGAAAGGATTATTAAACGGAATTGAGGTGATTATGGG
AAAACAGGAAAATGTGTTAGCCTTGGGACTTAATTTAGGGGATAATATCGAGCTTTTAAGAGCTGAAGTTGAAAAGATTG
TTAGAGAAAAGCTAAGAGAGAACAAGGAAGTAATAATTGCTGTGGATTTATTTGGGGGAAGTCCATTTAATGTTGCACTT
TATGTTATGAAAAATTACGATGTAAAAGTTATAACAGGAGTTAACATGCCTATGCTTATAGAATTGCTGTCTTCACTTAA
TGCATGTGACACAAAAGAACTGATAAATAACATATACAAAGTTGGTGTTGATGGTATTAAGGTAATTGAGAAGAATTCAC
TTAGTTTGTGA

Upstream 100 bases:

>100_bases
AATTTAAAATAAAGTATAGTTAACATCAACTTTTAAAAAAGTTTCAAAGAAGTATTTTAAAATTCAAAGTTAAAGATATT
AGCAAAGCGGAGGTGTAGTA

Downstream 100 bases:

>100_bases
AGAAAGAGAGGTGAAACAGCATACGAGTAAAAAAATAGCAGACAGTTGGAGGGAATACAGTCTAACTCGAGTAATAACAA
AGAAGGCTCTACAGAGGTAA

Product: PTS system fructose subfamily IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Fru; PTS system fructose-specific EIIA component; p16 [H]

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MKEKFVLIITHGNFGKGLLNGIEVIMGKQENVLALGLNLGDNIELLRAEVEKIVREKLRENKEVIIAVDLFGGSPFNVAL
YVMKNYDVKVITGVNMPMLIELLSSLNACDTKELINNIYKVGVDGIKVIEKNSLSL

Sequences:

>Translated_136_residues
MKEKFVLIITHGNFGKGLLNGIEVIMGKQENVLALGLNLGDNIELLRAEVEKIVREKLRENKEVIIAVDLFGGSPFNVAL
YVMKNYDVKVITGVNMPMLIELLSSLNACDTKELINNIYKVGVDGIKVIEKNSLSL
>Mature_136_residues
MKEKFVLIITHGNFGKGLLNGIEVIMGKQENVLALGLNLGDNIELLRAEVEKIVREKLRENKEVIIAVDLFGGSPFNVAL
YVMKNYDVKVITGVNMPMLIELLSSLNACDTKELINNIYKVGVDGIKVIEKNSLSL

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=133, Percent_Identity=34.5864661654135, Blast_Score=66, Evalue=7e-13,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15058; Mature: 15058

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKFVLIITHGNFGKGLLNGIEVIMGKQENVLALGLNLGDNIELLRAEVEKIVREKLRE
CCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
NKEVIIAVDLFGGSPFNVALYVMKNYDVKVITGVNMPMLIELLSSLNACDTKELINNIYK
CCEEEEEEEECCCCCCEEEEEEEECCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHH
VGVDGIKVIEKNSLSL
HCCCCEEEEECCCCCC
>Mature Secondary Structure
MKEKFVLIITHGNFGKGLLNGIEVIMGKQENVLALGLNLGDNIELLRAEVEKIVREKLRE
CCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
NKEVIIAVDLFGGSPFNVALYVMKNYDVKVITGVNMPMLIELLSSLNACDTKELINNIYK
CCEEEEEEEECCCCCCEEEEEEEECCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHH
VGVDGIKVIEKNSLSL
HCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377 [H]