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The map label for this gene is hpaD [H]

Identifier: 163845476

GI number: 163845476

Start: 1353144

End: 1354124

Strand: Reverse

Name: hpaD [H]

Synonym: BSUIS_B1390

Alternate gene names: 163845476

Gene position: 1354124-1353144 (Counterclockwise)

Preceding gene: 163845477

Following gene: 163845475

Centisome position: 96.66

GC content: 59.43

Gene sequence:

>981_bases
ATGCCCTTGCCGACCCCTAACCTTCACCCACCATTCAACATTGTGCGCCTGTCGCATGTGGAGCTTGGGGTGACAGACCT
TGCCAAGTCGCGGGCTTTCTATGTTGATACGCTTGGTCTGCAAGTGACCGACGAAAGCGCAGATGCCATTTATCTGCGCG
CCCTGGAAGAGCGCGGGCATCATTGCATCGTTTTGCGCAAGACCGACAAGGCGGAAGCCCGCGATCTTGGCTTCAAGCTC
TATTCGGAGGACGATCTCGACCGGGCCGAACATTTCTTCAAGGGCAAGGGCCTGCCTGTCGAATGGGTGGAGCGACCCTA
CCAGTCGCGCACGCTGCGCACGCGTGATCCGCACGGCATTCCGCTTGAGTTCTATTCCAGAATGGACCGCCTGCCGCCGA
TCCATCAAAAATATGCGCTCTATAAGGGCGTGAAGCCGCTGCGCATCGACCACTTCAACTGCTTCTCGCCTAATGTGGAT
GAATCCGTCGCCTTCTATAACGAGCTGGGTTTCCGCGTGACGGAATATACCGAGGATGCCGAAACTGGCCGCCTGTGGGC
CGCCTGGACGCACCGCAAGGGCGGCGTTCACGATATCGCCTTCACCAATGGCAAGGGGCCGCGCCTGCACCATACCGCTT
TCTGGGTGCCGACACCGCTCAACATCATCGACCTGCTCGACCTCATGTCCACCACCGGCTGGCTTGCCAATATCGAGCGC
GGTCCCGGCCGCCACGGCATTTCCAATGCCTTCTTCCTCTATGTGCGCGACCCGGATGGCCATCGCATCGAAATCTACTG
CTCGGATTACCAGACGGTCGATCCCGATCTGGAGCCGATCAAGTGGGATCTGAAAGACCCGCAGCGCCAGACGCTCTGGG
GTGCGCCTGCACCGAAATCCTGGTTCGAGGAAGGCAGCTTGTTTGCCAGTGTGCAGCCGGTCGATTCCGTGTTGAAGGCA
CAGCCGATCGTCGCGCCGTAA

Upstream 100 bases:

>100_bases
CGCCTTCGCCAACGCTGCGCACGCGATTGCAAAACTCGGCGGCTGAGCACGGGCATTCAAACCCCGCATCGCCGCATCTG
ACATATTGAGGAGGAAAATC

Downstream 100 bases:

>100_bases
TGGGCGACCTTGATGCCACCCGCCTATGCAGGCGGGTGGTGCGGGACGGAAGGAAATCTGAGGAGATCATGGTGAAGTTT
CTGAGTTTTTCGACCTGCGG

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase; Mn(II)-dependent 23OHBP oxygenase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MPLPTPNLHPPFNIVRLSHVELGVTDLAKSRAFYVDTLGLQVTDESADAIYLRALEERGHHCIVLRKTDKAEARDLGFKL
YSEDDLDRAEHFFKGKGLPVEWVERPYQSRTLRTRDPHGIPLEFYSRMDRLPPIHQKYALYKGVKPLRIDHFNCFSPNVD
ESVAFYNELGFRVTEYTEDAETGRLWAAWTHRKGGVHDIAFTNGKGPRLHHTAFWVPTPLNIIDLLDLMSTTGWLANIER
GPGRHGISNAFFLYVRDPDGHRIEIYCSDYQTVDPDLEPIKWDLKDPQRQTLWGAPAPKSWFEEGSLFASVQPVDSVLKA
QPIVAP

Sequences:

>Translated_326_residues
MPLPTPNLHPPFNIVRLSHVELGVTDLAKSRAFYVDTLGLQVTDESADAIYLRALEERGHHCIVLRKTDKAEARDLGFKL
YSEDDLDRAEHFFKGKGLPVEWVERPYQSRTLRTRDPHGIPLEFYSRMDRLPPIHQKYALYKGVKPLRIDHFNCFSPNVD
ESVAFYNELGFRVTEYTEDAETGRLWAAWTHRKGGVHDIAFTNGKGPRLHHTAFWVPTPLNIIDLLDLMSTTGWLANIER
GPGRHGISNAFFLYVRDPDGHRIEIYCSDYQTVDPDLEPIKWDLKDPQRQTLWGAPAPKSWFEEGSLFASVQPVDSVLKA
QPIVAP
>Mature_325_residues
PLPTPNLHPPFNIVRLSHVELGVTDLAKSRAFYVDTLGLQVTDESADAIYLRALEERGHHCIVLRKTDKAEARDLGFKLY
SEDDLDRAEHFFKGKGLPVEWVERPYQSRTLRTRDPHGIPLEFYSRMDRLPPIHQKYALYKGVKPLRIDHFNCFSPNVDE
SVAFYNELGFRVTEYTEDAETGRLWAAWTHRKGGVHDIAFTNGKGPRLHHTAFWVPTPLNIIDLLDLMSTTGWLANIERG
PGRHGISNAFFLYVRDPDGHRIEIYCSDYQTVDPDLEPIKWDLKDPQRQTLWGAPAPKSWFEEGSLFASVQPVDSVLKAQ
PIVAP

Specific function: Catalyzes the meta-cleavage of the hydroxylated biphenyl ring. The enzyme can oxidize a wide range of substrates, and the substrate preference order is 2,3-dihydroxybiphenyl > 3- methylcatechol > catechol > 4-methylcatechol > 4-chlorocatechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =1.13.11.39 [H]

Molecular weight: Translated: 37298; Mature: 37166

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00082 EXTRADIOL_DIOXYGENAS ; PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPTPNLHPPFNIVRLSHVELGVTDLAKSRAFYVDTLGLQVTDESADAIYLRALEERGH
CCCCCCCCCCCEEEEEEEEEEECHHHHHHCCEEEEEECCEEEECCCCCEEEEEEHHHCCC
HCIVLRKTDKAEARDLGFKLYSEDDLDRAEHFFKGKGLPVEWVERPYQSRTLRTRDPHGI
EEEEEEECCCCCHHHCCEEEECCCCHHHHHHHHCCCCCCHHHHHCCHHCCCCCCCCCCCC
PLEFYSRMDRLPPIHQKYALYKGVKPLRIDHFNCFSPNVDESVAFYNELGFRVTEYTEDA
CHHHHHHHHCCCCHHHHHHHHHCCCCEEECCEECCCCCCCHHHHHHHHHCCEEEEECCCC
ETGRLWAAWTHRKGGVHDIAFTNGKGPRLHHTAFWVPTPLNIIDLLDLMSTTGWLANIER
CCCEEEEEEECCCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEECCC
GPGRHGISNAFFLYVRDPDGHRIEIYCSDYQTVDPDLEPIKWDLKDPQRQTLWGAPAPKS
CCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHCCCCCCCHH
WFEEGSLFASVQPVDSVLKAQPIVAP
HHHCCCEEEECCHHHHHHHCCCCCCC
>Mature Secondary Structure 
PLPTPNLHPPFNIVRLSHVELGVTDLAKSRAFYVDTLGLQVTDESADAIYLRALEERGH
CCCCCCCCCCEEEEEEEEEEECHHHHHHCCEEEEEECCEEEECCCCCEEEEEEHHHCCC
HCIVLRKTDKAEARDLGFKLYSEDDLDRAEHFFKGKGLPVEWVERPYQSRTLRTRDPHGI
EEEEEEECCCCCHHHCCEEEECCCCHHHHHHHHCCCCCCHHHHHCCHHCCCCCCCCCCCC
PLEFYSRMDRLPPIHQKYALYKGVKPLRIDHFNCFSPNVDESVAFYNELGFRVTEYTEDA
CHHHHHHHHCCCCHHHHHHHHHCCCCEEECCEECCCCCCCHHHHHHHHHCCEEEEECCCC
ETGRLWAAWTHRKGGVHDIAFTNGKGPRLHHTAFWVPTPLNIIDLLDLMSTTGWLANIER
CCCEEEEEEECCCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEECCC
GPGRHGISNAFFLYVRDPDGHRIEIYCSDYQTVDPDLEPIKWDLKDPQRQTLWGAPAPKS
CCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCCCCCHHHCCCCCCCHH
WFEEGSLFASVQPVDSVLKAQPIVAP
HHHCCCEEEECCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12672826 [H]