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The map label for this gene is hpaI [H]

Identifier: 163845472

GI number: 163845472

Start: 1350375

End: 1350998

Strand: Reverse

Name: hpaI [H]

Synonym: BSUIS_B1386

Alternate gene names: 163845472

Gene position: 1350998-1350375 (Counterclockwise)

Preceding gene: 163845473

Following gene: 163845465

Centisome position: 96.44

GC content: 61.22

Gene sequence:

>624_bases
ATGACGGGTTCAGCCTCGCATCCGGTCGTGCGGCTGCCTGTCGGCGAAACGTGGATCGTCAAGCAATTGCTCGATATCGG
CGCGCAGACCCTGCTGATCCCCATGATTGAAAGCCGCGAACAGGCCGAAGCCATGGTAAAAGCCATGCGCTATCCGCCGC
ATGGGGTGCGTGGTGTCGGTTCCGCTCTGGCGCGGGCTTCTGCCTTCAACCGTATCCCCGATTATCTACAGACGGCCAAT
GACGAGGTTTGTCTTCTGTTGCAGGTGGAAAGCCGCGCGGGGCTTGCCAATCTTGATGCAATTGCCACGACCGAAGGCGT
GGACGGCGTCTTCATCGGCCCTGCCGATCTTGCTGCCGATATGGGGCATCTGGGCAAACCGGGCGCGCAAGAGGTGCAGG
ACGCTGTGCGCGACGCACTTCAACGCATCATCGGGCACGGCAAGGCGGCGGGCATTCTCACGGGCGACCTTTCGCTTGCC
CGTCATTATCTCGACCTTGGCGCAACCTTTGTCGCCATCGGCAATGATGTGACCTTGCTCGCGAATGCCACCACTAGGCT
GCTTGCCGACTTCAAGACCACTGAGCCGGGCAGGCCAGCCAGCCCGGACGCAAGGGTCTATTGA

Upstream 100 bases:

>100_bases
TACGGTCGAAATCTGTGCCGGAGCCGGATATGACTGGCTGCTGCTCGATGGCGAACATGCGCCCAATGATGTGCCTCTTC
TGGTTTCACAGCTTCAGGCC

Downstream 100 bases:

>100_bases
GCCTGACAGGCCGCCTGTTTCATTTCGGCAAGGTGAAAAGTGCAGTTCCAGACAAAAGGAAGTGCAGGACCAGAGGGGTG
CCCGTTGAGGCCGGGTTGGC

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MTGSASHPVVRLPVGETWIVKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRGVGSALARASAFNRIPDYLQTAN
DEVCLLLQVESRAGLANLDAIATTEGVDGVFIGPADLAADMGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLA
RHYLDLGATFVAIGNDVTLLANATTRLLADFKTTEPGRPASPDARVY

Sequences:

>Translated_207_residues
MTGSASHPVVRLPVGETWIVKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRGVGSALARASAFNRIPDYLQTAN
DEVCLLLQVESRAGLANLDAIATTEGVDGVFIGPADLAADMGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLA
RHYLDLGATFVAIGNDVTLLANATTRLLADFKTTEPGRPASPDARVY
>Mature_206_residues
TGSASHPVVRLPVGETWIVKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRGVGSALARASAFNRIPDYLQTAND
EVCLLLQVESRAGLANLDAIATTEGVDGVFIGPADLAADMGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLAR
HYLDLGATFVAIGNDVTLLANATTRLLADFKTTEPGRPASPDARVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=192, Percent_Identity=48.9583333333333, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1788578, Length=193, Percent_Identity=49.740932642487, Blast_Score=186, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 21830; Mature: 21699

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSASHPVVRLPVGETWIVKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRGVG
CCCCCCCCEEECCCCCHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHHCCCCCCHHHHH
SALARASAFNRIPDYLQTANDEVCLLLQVESRAGLANLDAIATTEGVDGVFIGPADLAAD
HHHHHHHHHHHCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCEEEECHHHHHHH
MGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLARHYLDLGATFVAIGNDVTLL
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCEEEEECCCEEEE
ANATTRLLADFKTTEPGRPASPDARVY
EHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGSASHPVVRLPVGETWIVKQLLDIGAQTLLIPMIESREQAEAMVKAMRYPPHGVRGVG
CCCCCCCEEECCCCCHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHHCCCCCCHHHHH
SALARASAFNRIPDYLQTANDEVCLLLQVESRAGLANLDAIATTEGVDGVFIGPADLAAD
HHHHHHHHHHHCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCEEEECHHHHHHH
MGHLGKPGAQEVQDAVRDALQRIIGHGKAAGILTGDLSLARHYLDLGATFVAIGNDVTLL
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCEEEEECCCEEEE
ANATTRLLADFKTTEPGRPASPDARVY
EHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA