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The map label for this gene is livK [C]

Identifier: 163845363

GI number: 163845363

Start: 1223379

End: 1224494

Strand: Reverse

Name: livK [C]

Synonym: BSUIS_B1262

Alternate gene names: 163845363

Gene position: 1224494-1223379 (Counterclockwise)

Preceding gene: 163845364

Following gene: 163845361

Centisome position: 87.41

GC content: 54.75

Gene sequence:

>1116_bases
ATGAGAAAAACACTTTTTTCGGGCGTTGCTCTTGCTGCAGTCATCGCCTTCGGGGGCTCGGCTTGGGCTGATGTACTGGT
CGGTATCGGTATTCCTGTAACCGGCCCGAACGCCGTTTATGGTGCACAGATCCAGAAAGGCGCCGAGGCGGCAATCAAGG
AAGTCAACGATGCCGGCGGCATCAATGGCGAGAAGATTGCCATCACAATCGGCGATGACGTATCCGATCCGAAGCAGGGT
ATTTCGGTGGCTAATAAATTTGCCGCCGATGGCGTCAAGTTCGTGATTGGACACTTCAACTCTGGCGTTACCATTCCGGC
TTCGCAGGTCTATGCCGAAAACGGTATTCTCGAAATTTCGCCTGGCGCGACCAATCCTCAATATACGGAGCAGGGGCTGT
GGAACACATTCCGCACCTGCGGCCGTGATGATCAGCAGGGCACTGTTGCCGGTCAGTATATTTTTGACCACTTCAAGGAT
GCGAAGATCGCCGTCATTCACGACAAGACCCCTTACGGTCAGGGGCTTGCCGACGAAACCAAGAAGAAGCTGAATGAACT
TGGCACCAAGGAAACCCTCTATGAAGGCGTGAACGTTGGTGAAAAGGATTTCTCGGCGCTGATCGCCAAGCTCAAGCAGG
CTGGCGTGAACGTGGTTTATTGGGGTGGCCTGCACCCGGAAGCCGGTCTCATCATCCGTCAGATGGCCGATCAGGGACTA
AAAGCCCAGTTCATTTCGGGTGACGGTATTGTGTCGAACGATCTGGCGTCAATCGCCGGTCCGGCTGTCGAAGGCACGCT
CAACACCTTCGGTCCCGATCCGCGCAACAACCCGGATAATGCCGAACTGGTGAAGAAATTCCGCGACGCCGGTTTTGAGC
CGGAAGCCTATACGCTCTATTCCTATGCGGCGGTTCAGTCGCTGGCTCAGGCCGCAAAGGCCGCTGGCAGCAATGATCCG
CAGGAAGTCGCCAAAGCCATGAAGGAAAAGGGCCCGTTCAAGACCGTGCTCGGCGATCTGTCCTATGATGAAAAGGGTGA
TCCGAAGCTTCCGGGCTATGTCATGTACAAGTGGGAAAAGGGTGCAGACGGGAAGTATAATTACATTCAGCAGTAA

Upstream 100 bases:

>100_bases
AATAATAAGATTCAACGTAGATATTGTCGATTAATCGACAAGATAATAAGGCGATGCGAAAACATCGCTTCGGGGTAAAC
AAAATTTGGGAGTTCCAACA

Downstream 100 bases:

>100_bases
GTTTCCGTCATATTCTTTTCTGAAAATATCAGATGCCCGGCTTTTGAAGCCGGGCATTTTCAATTGCAATCCGCCTTCCC
GCATAAAGTCATGCGAAAAA

Product: hypothetical protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRKTLFSGVALAAVIAFGGSAWADVLVGIGIPVTGPNAVYGAQIQKGAEAAIKEVNDAGGINGEKIAITIGDDVSDPKQG
ISVANKFAADGVKFVIGHFNSGVTIPASQVYAENGILEISPGATNPQYTEQGLWNTFRTCGRDDQQGTVAGQYIFDHFKD
AKIAVIHDKTPYGQGLADETKKKLNELGTKETLYEGVNVGEKDFSALIAKLKQAGVNVVYWGGLHPEAGLIIRQMADQGL
KAQFISGDGIVSNDLASIAGPAVEGTLNTFGPDPRNNPDNAELVKKFRDAGFEPEAYTLYSYAAVQSLAQAAKAAGSNDP
QEVAKAMKEKGPFKTVLGDLSYDEKGDPKLPGYVMYKWEKGADGKYNYIQQ

Sequences:

>Translated_371_residues
MRKTLFSGVALAAVIAFGGSAWADVLVGIGIPVTGPNAVYGAQIQKGAEAAIKEVNDAGGINGEKIAITIGDDVSDPKQG
ISVANKFAADGVKFVIGHFNSGVTIPASQVYAENGILEISPGATNPQYTEQGLWNTFRTCGRDDQQGTVAGQYIFDHFKD
AKIAVIHDKTPYGQGLADETKKKLNELGTKETLYEGVNVGEKDFSALIAKLKQAGVNVVYWGGLHPEAGLIIRQMADQGL
KAQFISGDGIVSNDLASIAGPAVEGTLNTFGPDPRNNPDNAELVKKFRDAGFEPEAYTLYSYAAVQSLAQAAKAAGSNDP
QEVAKAMKEKGPFKTVLGDLSYDEKGDPKLPGYVMYKWEKGADGKYNYIQQ
>Mature_371_residues
MRKTLFSGVALAAVIAFGGSAWADVLVGIGIPVTGPNAVYGAQIQKGAEAAIKEVNDAGGINGEKIAITIGDDVSDPKQG
ISVANKFAADGVKFVIGHFNSGVTIPASQVYAENGILEISPGATNPQYTEQGLWNTFRTCGRDDQQGTVAGQYIFDHFKD
AKIAVIHDKTPYGQGLADETKKKLNELGTKETLYEGVNVGEKDFSALIAKLKQAGVNVVYWGGLHPEAGLIIRQMADQGL
KAQFISGDGIVSNDLASIAGPAVEGTLNTFGPDPRNNPDNAELVKKFRDAGFEPEAYTLYSYAAVQSLAQAAKAAGSNDP
QEVAKAMKEKGPFKTVLGDLSYDEKGDPKLPGYVMYKWEKGADGKYNYIQQ

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=366, Percent_Identity=41.2568306010929, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI48994941, Length=359, Percent_Identity=40.6685236768802, Blast_Score=251, Evalue=6e-68,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39387; Mature: 39387

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTLFSGVALAAVIAFGGSAWADVLVGIGIPVTGPNAVYGAQIQKGAEAAIKEVNDAGG
CCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCC
INGEKIAITIGDDVSDPKQGISVANKFAADGVKFVIGHFNSGVTIPASQVYAENGILEIS
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEEC
PGATNPQYTEQGLWNTFRTCGRDDQQGTVAGQYIFDHFKDAKIAVIHDKTPYGQGLADET
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
KKKLNELGTKETLYEGVNVGEKDFSALIAKLKQAGVNVVYWGGLHPEAGLIIRQMADQGL
HHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCC
KAQFISGDGIVSNDLASIAGPAVEGTLNTFGPDPRNNPDNAELVKKFRDAGFEPEAYTLY
EEEEECCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
SYAAVQSLAQAAKAAGSNDPQEVAKAMKEKGPFKTVLGDLSYDEKGDPKLPGYVMYKWEK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEC
GADGKYNYIQQ
CCCCCCCCCCC
>Mature Secondary Structure
MRKTLFSGVALAAVIAFGGSAWADVLVGIGIPVTGPNAVYGAQIQKGAEAAIKEVNDAGG
CCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCC
INGEKIAITIGDDVSDPKQGISVANKFAADGVKFVIGHFNSGVTIPASQVYAENGILEIS
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEEC
PGATNPQYTEQGLWNTFRTCGRDDQQGTVAGQYIFDHFKDAKIAVIHDKTPYGQGLADET
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHH
KKKLNELGTKETLYEGVNVGEKDFSALIAKLKQAGVNVVYWGGLHPEAGLIIRQMADQGL
HHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCC
KAQFISGDGIVSNDLASIAGPAVEGTLNTFGPDPRNNPDNAELVKKFRDAGFEPEAYTLY
EEEEECCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
SYAAVQSLAQAAKAAGSNDPQEVAKAMKEKGPFKTVLGDLSYDEKGDPKLPGYVMYKWEK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEC
GADGKYNYIQQ
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA