Click here to switch to the map view.

The map label for this gene is livK [C]

Identifier: 163845359

GI number: 163845359

Start: 1221643

End: 1222758

Strand: Reverse

Name: livK [C]

Synonym: BSUIS_B1258

Alternate gene names: 163845359

Gene position: 1222758-1221643 (Counterclockwise)

Preceding gene: 163845360

Following gene: 163845358

Centisome position: 87.29

GC content: 56.72

Gene sequence:

>1116_bases
ATGAAGAAATCCTTATTTTGCGGCGTTTGCCTTTGTGCGCTGGTCGCGATGGGCGGGACATCTTTCGCCGATATCATGGT
CGGTGTTGGTGCGCCCCTGACTGGCAGCCAGGCTGCCTTCGGCGAACAGATCAAGCGCGGCGTGGAGGCGGCTGTCGCGG
AAGCCAATGCCAAGGGCGGCATGAATGGTGAGCAGATCACCCTCGTTTATGGTGATGACGCTGCCGATCCCAAGCAGGGC
ATCTCGGTTGCAAACAAGTTCGTGGGCGATGGCGTCAAGTTTGTGATTGGCCATTTCAATTCCGGTGTCAGCATTCCGAC
CTCGGATATCTATGCCGAAAACGGCGTGCTGATGATTGCACCGGGCACGACCAACCCGACCTTTACCGAGCGCGAATTGT
GGAACACATTCCGCACCTGCCGCCGCGACGACAAGCAGGGCATTGTCGCAGGCAAGTACATGGCCGATAATTACAAGGAC
GGCAAGGTTGCCATTCTGCACGACAAGACGCCCTATGGTCAGGGCCTTGCCGACGAAACTAAAAAGTCGCTCAACGAGAA
CGGCATGAAGGAAACGCTCTATGAGGGCGTGAATCAGGGCGACAAGGATTTCTCCGCGCTCATTTCCAAGATGAAAGCTG
CGGGCATCACTGCCGTTTACTGGGGCGGTATGCACCCGGAAGCTGGCCTTCTGATTCGCCAGATGGCCGATCAGGGGCTG
AAAGCCCAGTTCATTTCGGGTGACGGCATCGTGTCCAACGAGCTGGCGTCGATTGCCGGTGACGCCGTGGCCGGTGTGAT
GAATACTTTCGGTCCCGATCCGCGCGACGACAAGGCCAATGCCGAACTCATCAAGGCGTTCCGTGACAAGGGTTTCGAGC
CGGAAGCCTATACGTTCTATGCCTATGCCGGTGTGCAATCGCTTGTGAACGCGGCCAATGCGGCTGGCAGCAATGATCCG
ATGGAAGTCGCTACCGCGATGAAGGAAAAGGGTCCGTTCAAGACCGTGCTCGGCGATATCTCCTTCGATGCGAAGGGTGA
TCCGAGCCTGTCGCCCTATGTCATGTTTGAATGGCGCAAGGGCGAAGACGGCAAGTATAATTACTTCCAGAAGTAA

Upstream 100 bases:

>100_bases
AATTCTGCAAGGCATCGGGCGGTTCGAGGAATCTGCCCTGAAATTTGCGGTTCGCACTGCGTCGGGCGGCGAACCGCCGT
TCATAATGGGAGCAATCGAA

Downstream 100 bases:

>100_bases
ATTGCCTGATATATTAGCAAAAAATGCTACAAAATTCAGCAATCTGCCCGGCTTTTAGCCGGGCATTTTGTTTTTAACTT
CCCGTTGCACAGTTTGTTTG

Product: hypothetical protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKKSLFCGVCLCALVAMGGTSFADIMVGVGAPLTGSQAAFGEQIKRGVEAAVAEANAKGGMNGEQITLVYGDDAADPKQG
ISVANKFVGDGVKFVIGHFNSGVSIPTSDIYAENGVLMIAPGTTNPTFTERELWNTFRTCRRDDKQGIVAGKYMADNYKD
GKVAILHDKTPYGQGLADETKKSLNENGMKETLYEGVNQGDKDFSALISKMKAAGITAVYWGGMHPEAGLLIRQMADQGL
KAQFISGDGIVSNELASIAGDAVAGVMNTFGPDPRDDKANAELIKAFRDKGFEPEAYTFYAYAGVQSLVNAANAAGSNDP
MEVATAMKEKGPFKTVLGDISFDAKGDPSLSPYVMFEWRKGEDGKYNYFQK

Sequences:

>Translated_371_residues
MKKSLFCGVCLCALVAMGGTSFADIMVGVGAPLTGSQAAFGEQIKRGVEAAVAEANAKGGMNGEQITLVYGDDAADPKQG
ISVANKFVGDGVKFVIGHFNSGVSIPTSDIYAENGVLMIAPGTTNPTFTERELWNTFRTCRRDDKQGIVAGKYMADNYKD
GKVAILHDKTPYGQGLADETKKSLNENGMKETLYEGVNQGDKDFSALISKMKAAGITAVYWGGMHPEAGLLIRQMADQGL
KAQFISGDGIVSNELASIAGDAVAGVMNTFGPDPRDDKANAELIKAFRDKGFEPEAYTFYAYAGVQSLVNAANAAGSNDP
MEVATAMKEKGPFKTVLGDISFDAKGDPSLSPYVMFEWRKGEDGKYNYFQK
>Mature_371_residues
MKKSLFCGVCLCALVAMGGTSFADIMVGVGAPLTGSQAAFGEQIKRGVEAAVAEANAKGGMNGEQITLVYGDDAADPKQG
ISVANKFVGDGVKFVIGHFNSGVSIPTSDIYAENGVLMIAPGTTNPTFTERELWNTFRTCRRDDKQGIVAGKYMADNYKD
GKVAILHDKTPYGQGLADETKKSLNENGMKETLYEGVNQGDKDFSALISKMKAAGITAVYWGGMHPEAGLLIRQMADQGL
KAQFISGDGIVSNELASIAGDAVAGVMNTFGPDPRDDKANAELIKAFRDKGFEPEAYTFYAYAGVQSLVNAANAAGSNDP
MEVATAMKEKGPFKTVLGDISFDAKGDPSLSPYVMFEWRKGEDGKYNYFQK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=361, Percent_Identity=38.2271468144044, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI48994941, Length=359, Percent_Identity=38.4401114206128, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39517; Mature: 39517

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLFCGVCLCALVAMGGTSFADIMVGVGAPLTGSQAAFGEQIKRGVEAAVAEANAKGG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
MNGEQITLVYGDDAADPKQGISVANKFVGDGVKFVIGHFNSGVSIPTSDIYAENGVLMIA
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCCEEEEE
PGTTNPTFTERELWNTFRTCRRDDKQGIVAGKYMADNYKDGKVAILHDKTPYGQGLADET
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHH
KKSLNENGMKETLYEGVNQGDKDFSALISKMKAAGITAVYWGGMHPEAGLLIRQMADQGL
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCC
KAQFISGDGIVSNELASIAGDAVAGVMNTFGPDPRDDKANAELIKAFRDKGFEPEAYTFY
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEE
AYAGVQSLVNAANAAGSNDPMEVATAMKEKGPFKTVLGDISFDAKGDPSLSPYVMFEWRK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEC
GEDGKYNYFQK
CCCCCCCCCCC
>Mature Secondary Structure
MKKSLFCGVCLCALVAMGGTSFADIMVGVGAPLTGSQAAFGEQIKRGVEAAVAEANAKGG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
MNGEQITLVYGDDAADPKQGISVANKFVGDGVKFVIGHFNSGVSIPTSDIYAENGVLMIA
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCCEEEEE
PGTTNPTFTERELWNTFRTCRRDDKQGIVAGKYMADNYKDGKVAILHDKTPYGQGLADET
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHH
KKSLNENGMKETLYEGVNQGDKDFSALISKMKAAGITAVYWGGMHPEAGLLIRQMADQGL
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCC
KAQFISGDGIVSNELASIAGDAVAGVMNTFGPDPRDDKANAELIKAFRDKGFEPEAYTFY
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEE
AYAGVQSLVNAANAAGSNDPMEVATAMKEKGPFKTVLGDISFDAKGDPSLSPYVMFEWRK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEEEC
GEDGKYNYFQK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA