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The map label for this gene is tilS [H]

Identifier: 163845272

GI number: 163845272

Start: 1144270

End: 1145616

Strand: Reverse

Name: tilS [H]

Synonym: BSUIS_B1168

Alternate gene names: 163845272

Gene position: 1145616-1144270 (Counterclockwise)

Preceding gene: 163845273

Following gene: 163845271

Centisome position: 81.78

GC content: 61.17

Gene sequence:

>1347_bases
GTGGGGCTTTCCCCTGTCAATATCTTCAAGCCGTTCGGTCTGGGGCGTGCGAAAGCGGTGATTGCTGCCGTTTCGGGCGG
CAGCGATTCGCTTGGCCTTCTCTTTCTGCTGAAGGATTATCTTTCGACGCTTGAAAGCCCGCCGGTTCTGATCGCTGTGA
CGGTGGATCATAAACTGCGGGCGGAATCGGCGCTTGAAGCCGAAAATGTCGGCCTGCTCTGCCAAAAACACGGTATCATG
CATTGTGTCCTGTCATGGGACGATCCCAAACCGGCCCATGGGCTGGCGGCTGCGGCGCGCACGGCGCGTTACCGGCTTCT
GGTACAGGCGGCGCGGGATGCGGGCGCTGGTTTCATCGTGACTGGCCATACCGAGAATGACCAGATCGAAACTTTCCTGA
TGCGTAAGGCGCGTAGCGGCCATTGCGAGGCGCGCGGGCTCGCCGCCATGTCGCCGCGTTCGCTGCTGGAGGGTTCGGTC
GAGCTTGCCCGGCCCCTTCTCACAGTCTCGCGACAGGCTTTGCGCGACGAGCTGACGAGGCGGGGTATTGCATGGGTCGA
TGATCCGAGCAACGCCAATATCGACTATGAGCGCCCACGCGTGCGGCTGGGTGTGGCCGCCGAAGCCGACGGCCAGGAGG
TGCTGGAGCAGATTGCACAGGCTGGTGCGGCGCGTGAACGTGACAATGCGGCCCTGGTGGAGGCGCTCGCCGATCCCGCG
ACGCTTGGAGTGGATGCGGCCGGTATGATGTTCCTCAATGCCGACTGCTATGCAGCACTTTCACCAGGTGCACGGCAGCT
TTTCTCCGGCCTGCTTGCCTCTATCGCCGGGGGGCGGCGGTTTCTGCCCGGTGACGGCGAGCGTCGCCGTATCGAACGGA
TGTTGTCGGGGCAGGATGCACCCCGCCGCCTTACGGTTTTTGGCGCCCTGATCGAGCGTGGGGAGAAGGGGGCGCCTCAT
CGCTTCCGGCGCGAGCGTCGCAATCTGCCGAAGCTGGACCTTGTTCCCGGCCAGCATATCGTGTGGGACGGCAGGTTTTG
TTTTTTCAATTCAGGGGGCAGAAGTTTTGAAATTGCGCCTCCCGGACGGCAGGAACTGATCGATTTCCTGAAGAATAGCG
GCAGGGATATAGAATCCCGGCGATGCGAGGCGTTGCTGATTTCGCCTGCTCTTTATGAGGGTGGAAAACTTGCCTCCGTT
CCATTCCTGCCCGGAGCGGAATGGCCGCAAGGGGTTCATATCGAACGACATTTTGCGATTTTTGATCATGTCCTGCCCGG
CCATGATTTTGCGCTTGCACAGGCTGTGGAGGCGCGCCTTGGTCGCGCTTGCGCCGAAATATCCTAA

Upstream 100 bases:

>100_bases
CGCGCAGATTCCCCAACGCTATCCCAAGGCGGCGCCAGCCATTTTGAAGCGTGTCGCCGATGAGCGCAGTCGCGCCAAGT
GCTGATTGCGGAGTAAGTTC

Downstream 100 bases:

>100_bases
TTTTTGTAAACCAGAGCGCGAAGAATCGCGGATGCAGGGACGAACGCGCCTTGGCAACGCCTTCCTTCGATCCTATGTTA
GGACGAAACATCGTTCCGCG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MGLSPVNIFKPFGLGRAKAVIAAVSGGSDSLGLLFLLKDYLSTLESPPVLIAVTVDHKLRAESALEAENVGLLCQKHGIM
HCVLSWDDPKPAHGLAAAARTARYRLLVQAARDAGAGFIVTGHTENDQIETFLMRKARSGHCEARGLAAMSPRSLLEGSV
ELARPLLTVSRQALRDELTRRGIAWVDDPSNANIDYERPRVRLGVAAEADGQEVLEQIAQAGAARERDNAALVEALADPA
TLGVDAAGMMFLNADCYAALSPGARQLFSGLLASIAGGRRFLPGDGERRRIERMLSGQDAPRRLTVFGALIERGEKGAPH
RFRRERRNLPKLDLVPGQHIVWDGRFCFFNSGGRSFEIAPPGRQELIDFLKNSGRDIESRRCEALLISPALYEGGKLASV
PFLPGAEWPQGVHIERHFAIFDHVLPGHDFALAQAVEARLGRACAEIS

Sequences:

>Translated_448_residues
MGLSPVNIFKPFGLGRAKAVIAAVSGGSDSLGLLFLLKDYLSTLESPPVLIAVTVDHKLRAESALEAENVGLLCQKHGIM
HCVLSWDDPKPAHGLAAAARTARYRLLVQAARDAGAGFIVTGHTENDQIETFLMRKARSGHCEARGLAAMSPRSLLEGSV
ELARPLLTVSRQALRDELTRRGIAWVDDPSNANIDYERPRVRLGVAAEADGQEVLEQIAQAGAARERDNAALVEALADPA
TLGVDAAGMMFLNADCYAALSPGARQLFSGLLASIAGGRRFLPGDGERRRIERMLSGQDAPRRLTVFGALIERGEKGAPH
RFRRERRNLPKLDLVPGQHIVWDGRFCFFNSGGRSFEIAPPGRQELIDFLKNSGRDIESRRCEALLISPALYEGGKLASV
PFLPGAEWPQGVHIERHFAIFDHVLPGHDFALAQAVEARLGRACAEIS
>Mature_447_residues
GLSPVNIFKPFGLGRAKAVIAAVSGGSDSLGLLFLLKDYLSTLESPPVLIAVTVDHKLRAESALEAENVGLLCQKHGIMH
CVLSWDDPKPAHGLAAAARTARYRLLVQAARDAGAGFIVTGHTENDQIETFLMRKARSGHCEARGLAAMSPRSLLEGSVE
LARPLLTVSRQALRDELTRRGIAWVDDPSNANIDYERPRVRLGVAAEADGQEVLEQIAQAGAARERDNAALVEALADPAT
LGVDAAGMMFLNADCYAALSPGARQLFSGLLASIAGGRRFLPGDGERRRIERMLSGQDAPRRLTVFGALIERGEKGAPHR
FRRERRNLPKLDLVPGQHIVWDGRFCFFNSGGRSFEIAPPGRQELIDFLKNSGRDIESRRCEALLISPALYEGGKLASVP
FLPGAEWPQGVHIERHFAIFDHVLPGHDFALAQAVEARLGRACAEIS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=192, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 48546; Mature: 48415

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSPVNIFKPFGLGRAKAVIAAVSGGSDSLGLLFLLKDYLSTLESPPVLIAVTVDHKLR
CCCCCCCHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHH
AESALEAENVGLLCQKHGIMHCVLSWDDPKPAHGLAAAARTARYRLLVQAARDAGAGFIV
HHHHHCCCCCCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TGHTENDQIETFLMRKARSGHCEARGLAAMSPRSLLEGSVELARPLLTVSRQALRDELTR
ECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RGIAWVDDPSNANIDYERPRVRLGVAAEADGQEVLEQIAQAGAARERDNAALVEALADPA
CCCEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCH
TLGVDAAGMMFLNADCYAALSPGARQLFSGLLASIAGGRRFLPGDGERRRIERMLSGQDA
HCCCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
PRRLTVFGALIERGEKGAPHRFRRERRNLPKLDLVPGQHIVWDGRFCFFNSGGRSFEIAP
CCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEECCCCCEEEECCEEEEECCCCCEEEECC
PGRQELIDFLKNSGRDIESRRCEALLISPALYEGGKLASVPFLPGAEWPQGVHIERHFAI
CCHHHHHHHHHCCCCCCHHCCCCEEEECHHHCCCCCEEECCCCCCCCCCCCCEEEHHHHH
FDHVLPGHDFALAQAVEARLGRACAEIS
HHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GLSPVNIFKPFGLGRAKAVIAAVSGGSDSLGLLFLLKDYLSTLESPPVLIAVTVDHKLR
CCCCCCHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHH
AESALEAENVGLLCQKHGIMHCVLSWDDPKPAHGLAAAARTARYRLLVQAARDAGAGFIV
HHHHHCCCCCCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TGHTENDQIETFLMRKARSGHCEARGLAAMSPRSLLEGSVELARPLLTVSRQALRDELTR
ECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RGIAWVDDPSNANIDYERPRVRLGVAAEADGQEVLEQIAQAGAARERDNAALVEALADPA
CCCEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCH
TLGVDAAGMMFLNADCYAALSPGARQLFSGLLASIAGGRRFLPGDGERRRIERMLSGQDA
HCCCCCCCEEEECCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
PRRLTVFGALIERGEKGAPHRFRRERRNLPKLDLVPGQHIVWDGRFCFFNSGGRSFEIAP
CCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEECCCCCEEEECCEEEEECCCCCEEEECC
PGRQELIDFLKNSGRDIESRRCEALLISPALYEGGKLASVPFLPGAEWPQGVHIERHFAI
CCHHHHHHHHHCCCCCCHHCCCCEEEECHHHCCCCCEEECCCCCCCCCCCCCEEEHHHHH
FDHVLPGHDFALAQAVEARLGRACAEIS
HHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA