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The map label for this gene is purA [H]

Identifier: 163845263

GI number: 163845263

Start: 1133216

End: 1134505

Strand: Reverse

Name: purA [H]

Synonym: BSUIS_B1158

Alternate gene names: 163845263

Gene position: 1134505-1133216 (Counterclockwise)

Preceding gene: 163845264

Following gene: 163845262

Centisome position: 80.99

GC content: 60.85

Gene sequence:

>1290_bases
ATGGCAAATGTGGTTGTCGTCGGGTCGCAATGGGGCGACGAGGGCAAGGGAAAGATCGTTGACTGGCTGTCCGAGCGTGC
CGACGTGATCGTGCGCTACCAGGGCGGTCATAACGCAGGTCATACGCTTGTCATCGACGGTGTCAGCTACAAGCTTTCGC
TGCTGCCATCCGGCCTCGTGCGCGGCAAGTTGAGCGTTATCGGCAATGGCGTCGTGGTCGATCCGCACCATTTCGTCGCC
GAAGTTGAAAAGCTGCGCGGGCAGGGCATCGATGTGACGCCGGATGTGCTGCGCGTTGCGGAAAACGCACCGCTTATCCT
TTCGATCCATCGTGAACTCGACGCCATGCGCGAAGGCTCCAATTCAGGCCTCAAGATCGGCACGACCAAGCGCGGCATCG
GCCCCGCTTACGAAGACAAGGTTGGCCGCCGCGCCATCCGCGTGATCGATCTGACCGAGCCGGAAACATTGCGGCCGAAG
GTGGAGCGCCTGCTTGCGCACCATAATTCGCTGCGCCGCGGCATGGGCCTTGAAGAAATCGCCGTTGAAACCATCCTCAC
CGAACTGACTTCGGTTGCCGACCAGATCCTGCCCTATATCGATCAGGTCTGGCGTGTCCTGGACGAGCGCCGCAAGGCTG
GCGCCCGTATCCTGTTTGAAGGTGCGCAGGGCGCGCTGCTCGACAATGATCACGGCACCTATCCGTTCGTGACCTCTTCC
AACACGGTTGCCGGTCAGGCCGCTGCCGGTTCCGGCCTCGGCCCCACGGCTATCGGCTATGTGCTTGGCATCACCAAGGC
CTATACAACCCGCGTTGGCGAAGGTCCGTTCCCGACCGAGCTCAATGACGAGATCGGCGAATTCCTCGGCACCAAGGGCC
ATGAATTCGGCGTGGTGACGGGCCGCAAGCGCCGTTGCGGCTGGTTCGATGCCGTCATCGTGCGCCAGACAGTGCGCACT
TCCGGCATCAACGGTATTGCACTTACCAAGCTCGATGTTCTGGATGGTCTGGAAGAAATCAAGATTTGCGTCGCCTATGA
ACTCGACGGCAAGCGCATCGATTATCTGCCGTCTTCCATGGGCGCGCAGGCCCGCGTGAAGCCGATCTACGAAACCTTGC
CGGGCTGGTCTGAAACAACCGCCGGTGCGCGTTCGTGGAACGATCTTCCCGCGCAGACGGTCAAATATGTCCGCCATATC
GAAGAGCTGATCGGTGCGCCGGTGGCGATGCTCTCCACAAGCCCCGAGCGCGAGGATACGATCCTCGTAACCGATCCGTT
CCACGACTGA

Upstream 100 bases:

>100_bases
CTGTATAGAGACGAGGGATCAGCACCCTGTAGCCTCCGCTGGCAATGACCGGGCCGCTTTGATCCTTGGATTTTCAAATA
GAAGCCAAGGAGTTTCTTCA

Downstream 100 bases:

>100_bases
TTGTTTGATGATCCAGCCCGGCATGTCCGGGCTGGTTCGTTTCTGCCGCAAGCATTTCCAGCAAAAGTGTGAAACGGAGA
CGCTGGAAATGCTCTGGTTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLVRGKLSVIGNGVVVDPHHFVA
EVEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGSNSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPK
VERLLAHHNSLRRGMGLEEIAVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSS
NTVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVTGRKRRCGWFDAVIVRQTVRT
SGINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSMGAQARVKPIYETLPGWSETTAGARSWNDLPAQTVKYVRHI
EELIGAPVAMLSTSPEREDTILVTDPFHD

Sequences:

>Translated_429_residues
MANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLVRGKLSVIGNGVVVDPHHFVA
EVEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGSNSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPK
VERLLAHHNSLRRGMGLEEIAVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSS
NTVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVTGRKRRCGWFDAVIVRQTVRT
SGINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSMGAQARVKPIYETLPGWSETTAGARSWNDLPAQTVKYVRHI
EELIGAPVAMLSTSPEREDTILVTDPFHD
>Mature_428_residues
ANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLVRGKLSVIGNGVVVDPHHFVAE
VEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGSNSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPKV
ERLLAHHNSLRRGMGLEEIAVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSSN
TVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVTGRKRRCGWFDAVIVRQTVRTS
GINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSMGAQARVKPIYETLPGWSETTAGARSWNDLPAQTVKYVRHIE
ELIGAPVAMLSTSPEREDTILVTDPFHD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=423, Percent_Identity=45.3900709219858, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=42.3529411764706, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=43.6548223350254, Blast_Score=313, Evalue=3e-85,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=52.5700934579439, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=42.5233644859813, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=42.5233644859813, Blast_Score=325, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=41.7249417249417, Blast_Score=329, Evalue=5e-91,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=42.654028436019, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46592; Mature: 46461

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLV
CCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEEEEECCCHHH
RGKLSVIGNGVVVDPHHFVAEVEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGS
HCCCEEECCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCC
NSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPKVERLLAHHNSLRRGMGLEEI
CCCEEECCCCCCCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
AVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEECC
NTVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEE
GRKRRCGWFDAVIVRQTVRTSGINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSM
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHEEEEEEEECCCCEEECCCCCC
GAQARVKPIYETLPGWSETTAGARSWNDLPAQTVKYVRHIEELIGAPVAMLSTSPEREDT
CCCCEECHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCE
ILVTDPFHD
EEEECCCCC
>Mature Secondary Structure 
ANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLV
CCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEEEEECCCHHH
RGKLSVIGNGVVVDPHHFVAEVEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGS
HCCCEEECCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCC
NSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPKVERLLAHHNSLRRGMGLEEI
CCCEEECCCCCCCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
AVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEECC
NTVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEE
GRKRRCGWFDAVIVRQTVRTSGINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSM
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHEEEEEEEECCCCEEECCCCCC
GAQARVKPIYETLPGWSETTAGARSWNDLPAQTVKYVRHIEELIGAPVAMLSTSPEREDT
CCCCEECHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCE
ILVTDPFHD
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA