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The map label for this gene is dut

Identifier: 163845255

GI number: 163845255

Start: 1126308

End: 1126781

Strand: Reverse

Name: dut

Synonym: BSUIS_B1150

Alternate gene names: 163845255

Gene position: 1126781-1126308 (Counterclockwise)

Preceding gene: 163845256

Following gene: 163845254

Centisome position: 80.44

GC content: 61.39

Gene sequence:

>474_bases
ATGACCGCAGCATCCTCTTCCGCGCCAACGCTCGGCATCATTCGCCTCGAACATGCGAAGGGCCTTGATCTTCCCGCCTA
TGAAACCGCCGGTTCGGCGGGCATGGACCTGCGCGCCGCCGTGGCTGAAGATCGCCAGATCGTGCTCTTGCCCGGCCGCC
GCACGCTGGTGCCAACCGGCCTTATCCTTGAAATCCCGCAAGGCTATGAAGTACAGATCCGCCCCCGCTCGGGCCTTGCC
TTCAAGAATGGCATAACCTGCCTCAACACGCCCGGCACGATCGATTCGGATTATCGCGGCGAAGTAAAAGTCCTGCTGAT
CAATCTTGGAGATGACGATTTCCGCATCGAGCGCGGAATGCGCATCGCACAGGCCGTGTTCGCTCCGGTCATCCAGCCGA
AAATCGAGGAACGCGCCAAGATAAGTGAAACAGCCCGCGGCGCGGGCGGGTTCGGTTCCACCGGCACAGCCTGA

Upstream 100 bases:

>100_bases
TTGAAGTGCATCCTGAAAAGCGGTAAATGGCTTTCAGACAAGATACGCCTTGAAACACCACCTCGCAGGCCAACTGAATT
TTTGCAGGAAAGGCTCCCTC

Downstream 100 bases:

>100_bases
GGCCTATGCCCCACCAGATGAGAAAAGCCCGGCCCATGCGCCGGGCTTTTCTCTATGACAAATATTTAAGATGAAATTCC
GAGCCCGGAAGCATAGATAA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MTAASSSAPTLGIIRLEHAKGLDLPAYETAGSAGMDLRAAVAEDRQIVLLPGRRTLVPTGLILEIPQGYEVQIRPRSGLA
FKNGITCLNTPGTIDSDYRGEVKVLLINLGDDDFRIERGMRIAQAVFAPVIQPKIEERAKISETARGAGGFGSTGTA

Sequences:

>Translated_157_residues
MTAASSSAPTLGIIRLEHAKGLDLPAYETAGSAGMDLRAAVAEDRQIVLLPGRRTLVPTGLILEIPQGYEVQIRPRSGLA
FKNGITCLNTPGTIDSDYRGEVKVLLINLGDDDFRIERGMRIAQAVFAPVIQPKIEERAKISETARGAGGFGSTGTA
>Mature_156_residues
TAASSSAPTLGIIRLEHAKGLDLPAYETAGSAGMDLRAAVAEDRQIVLLPGRRTLVPTGLILEIPQGYEVQIRPRSGLAF
KNGITCLNTPGTIDSDYRGEVKVLLINLGDDDFRIERGMRIAQAVFAPVIQPKIEERAKISETARGAGGFGSTGTA

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=139, Percent_Identity=39.568345323741, Blast_Score=91, Evalue=3e-19,
Organism=Homo sapiens, GI4503423, Length=139, Percent_Identity=39.568345323741, Blast_Score=91, Evalue=4e-19,
Organism=Homo sapiens, GI70906441, Length=139, Percent_Identity=39.568345323741, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1790071, Length=137, Percent_Identity=45.2554744525547, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71988561, Length=151, Percent_Identity=43.046357615894, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319729, Length=135, Percent_Identity=40, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583610, Length=133, Percent_Identity=39.8496240601504, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921126, Length=133, Percent_Identity=39.8496240601504, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_BRUA1 (B2S7B5)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935536.1
- ProteinModelPortal:   B2S7B5
- SMR:   B2S7B5
- GeneID:   6327526
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I15780
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16660; Mature: 16529

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: BINDING 89-89 BINDING 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAASSSAPTLGIIRLEHAKGLDLPAYETAGSAGMDLRAAVAEDRQIVLLPGRRTLVPTG
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEECCC
LILEIPQGYEVQIRPRSGLAFKNGITCLNTPGTIDSDYRGEVKVLLINLGDDDFRIERGM
EEEECCCCCEEEEECCCCCEECCCEEEECCCCCCCCCCCCEEEEEEEEECCCCEEHHHHH
RIAQAVFAPVIQPKIEERAKISETARGAGGFGSTGTA
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TAASSSAPTLGIIRLEHAKGLDLPAYETAGSAGMDLRAAVAEDRQIVLLPGRRTLVPTG
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEECCC
LILEIPQGYEVQIRPRSGLAFKNGITCLNTPGTIDSDYRGEVKVLLINLGDDDFRIERGM
EEEECCCCCEEEEECCCCCEECCCEEEECCCCCCCCCCCCEEEEEEEEECCCCEEHHHHH
RIAQAVFAPVIQPKIEERAKISETARGAGGFGSTGTA
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA