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The map label for this gene is def [H]

Identifier: 163845148

GI number: 163845148

Start: 1009804

End: 1010367

Strand: Reverse

Name: def [H]

Synonym: BSUIS_B1030

Alternate gene names: 163845148

Gene position: 1010367-1009804 (Counterclockwise)

Preceding gene: 163845150

Following gene: 163845147

Centisome position: 72.13

GC content: 53.72

Gene sequence:

>564_bases
TTGACGATTCTCGCGCGACCCATTACGGAAAAGTCCATGTCTGTAAAACCGCTTATCATCCTTCCCGATCCCGTGCTGCG
TCAGGTTTCCAAGCCTGTTGAACGCTTCGACGATCAATTGCGCAAATTCGCCAGCGATATGTTCGACACCATGTATGATG
CGCCGGGCATTGGCCTTGCCGCCATTCAGGTGGGTGAACCGATCCGCATGCTTGTTATCGACCTTGCCAAGGAAGGCGAG
CCGAAAGCACCGCATATTTTCGTCAATCCGACGATCGTGCAGTCCTCCGACAAGCGCAGCACCTATGAAGAAGGCTGTCT
TTCGATCCCGGATTATTATGCGGAGGTCGAGCGCCCGGCGACGGTCAAGGTCAATTATTTCGATGCCGACGGCAAGCCGC
AGTCCATGGAGGCCGATGGCCTGATGGCCACCTGCCTTCAGCATGAAATTGACCATCTGAACGGTGTGCTCTTCATCGAC
CATATTTCCAAGCTGAAGCGCGATATGGTCATCAAGAAGTTCAAGAAGCTCGCCAGCCAGCGGGCATCGAAGAAAGTGCT
TTAA

Upstream 100 bases:

>100_bases
GAAGCGGCTCATTCAAGAGATTCTGGTTTTCCATAAAACAAGCTTAGCTGATTCGTGTCGCCTTGATGAGATCAAAACAG
GAACATTTTACAATGTGTAA

Downstream 100 bases:

>100_bases
TGCGTGTTGTTTTCATGGGGACGCCGGAATTTTCCGTGCCGATCCTCACCGCCATCATCGGTCACGGTTATGAGGTCGTT
GCAGCCTATACGCAGCCGCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTILARPITEKSMSVKPLIILPDPVLRQVSKPVERFDDQLRKFASDMFDTMYDAPGIGLAAIQVGEPIRMLVIDLAKEGE
PKAPHIFVNPTIVQSSDKRSTYEEGCLSIPDYYAEVERPATVKVNYFDADGKPQSMEADGLMATCLQHEIDHLNGVLFID
HISKLKRDMVIKKFKKLASQRASKKVL

Sequences:

>Translated_187_residues
MTILARPITEKSMSVKPLIILPDPVLRQVSKPVERFDDQLRKFASDMFDTMYDAPGIGLAAIQVGEPIRMLVIDLAKEGE
PKAPHIFVNPTIVQSSDKRSTYEEGCLSIPDYYAEVERPATVKVNYFDADGKPQSMEADGLMATCLQHEIDHLNGVLFID
HISKLKRDMVIKKFKKLASQRASKKVL
>Mature_186_residues
TILARPITEKSMSVKPLIILPDPVLRQVSKPVERFDDQLRKFASDMFDTMYDAPGIGLAAIQVGEPIRMLVIDLAKEGEP
KAPHIFVNPTIVQSSDKRSTYEEGCLSIPDYYAEVERPATVKVNYFDADGKPQSMEADGLMATCLQHEIDHLNGVLFIDH
ISKLKRDMVIKKFKKLASQRASKKVL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=32.484076433121, Blast_Score=87, Evalue=8e-18,
Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=44.1176470588235, Blast_Score=145, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=34.1772151898734, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=28.9308176100629, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21002; Mature: 20871

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILARPITEKSMSVKPLIILPDPVLRQVSKPVERFDDQLRKFASDMFDTMYDAPGIGLA
CCEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
AIQVGEPIRMLVIDLAKEGEPKAPHIFVNPTIVQSSDKRSTYEEGCLSIPDYYAEVERPA
EEECCCCHHHHHHHHHHCCCCCCCEEEECCEEECCCCCCCHHHHCCCCCCHHHHHCCCCC
TVKVNYFDADGKPQSMEADGLMATCLQHEIDHLNGVLFIDHISKLKRDMVIKKFKKLASQ
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHH
RASKKVL
HHHCCCC
>Mature Secondary Structure 
TILARPITEKSMSVKPLIILPDPVLRQVSKPVERFDDQLRKFASDMFDTMYDAPGIGLA
CEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
AIQVGEPIRMLVIDLAKEGEPKAPHIFVNPTIVQSSDKRSTYEEGCLSIPDYYAEVERPA
EEECCCCHHHHHHHHHHCCCCCCCEEEECCEEECCCCCCCHHHHCCCCCCHHHHHCCCCC
TVKVNYFDADGKPQSMEADGLMATCLQHEIDHLNGVLFIDHISKLKRDMVIKKFKKLASQ
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHH
RASKKVL
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]