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The map label for this gene is truA

Identifier: 163845146

GI number: 163845146

Start: 1008125

End: 1008880

Strand: Reverse

Name: truA

Synonym: BSUIS_B1028

Alternate gene names: 163845146

Gene position: 1008880-1008125 (Counterclockwise)

Preceding gene: 163845147

Following gene: 163845143

Centisome position: 72.02

GC content: 60.58

Gene sequence:

>756_bases
GTGCCGCGCTACAAGCTCACGGTTGAATATGACGGCACGCCCTATGTCGGCTGGCAGCGACAGGAAAACGGCCATGCGGT
GCAAGGCGCTATTGAGCAGGCGTTCAAGAAATTCTGTGGCGAAGACCTGACATTGAGTGCTGCGGGGCGCACCGATGCGG
GCGTTCATGCGACAGCGCAGGTTGCCCATGTCGATCTTGCCAAGGATTGGGGCGCGGGCAAGGTGCGCGATGCAGTCAAT
GCGCATCTGGTCATGGCGGATGAGCGCATCAGCATCCTGAATGTCGAGAAGACGACGGATACATTCGATGCGCGTTTTTC
CGCGCGTGCCCGGCATTATCTCTACCGGATTCATAATCGCCGCGCGCCGCTTGCAGTCGATTACCAGCGCGCATGGTGGG
TGCAGAAGCAGCTTGATGCTGACGCAATGCACGAGGCCGCGCAGCGGCTTCTGGGCGAACATGATTTCACCACCTTCCGC
GCCACGCAATGCCAGGCCAAAAGCCCGGTCAAGACGCTCGACCGGCTCGATGTTACCCGCAATGGCGACATGGTAGAAAT
GCGCGTTTCAGCGCGGTCCTTCCTGCACAATCAGGTGCGTTCCTTTGCGGGCAGCCTGATGGAAGTGGGCGTTGGCCGCT
GGACGGCCGATGATCTGCAAGCTGCCCTTGAGGCGCGTGACCGCAAGGCCTGCGGGCAGGTGGCGCCGCCCTATGGGCTT
TATCTGGTCGGAGTGGATTACGCCTTTCCGTTTTAG

Upstream 100 bases:

>100_bases
GGGCGCGGTGCGGCTTGCCACGCTGCAACATTCCGGCGGAAAACCCTTGCCTGCACAAGAGTTTCTGCGTGGGCAGCGCG
TCACGAAGGTTCTCTAGCCT

Downstream 100 bases:

>100_bases
ATGCGTATCAAAGCACGCTTCTCTCTTGCTCTTCGAGACGCCGTTTTCAACGGCTCCTCAGGATGAGGGGAAAACCGCGG
CATGGGAATATAACGCGCTC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MPRYKLTVEYDGTPYVGWQRQENGHAVQGAIEQAFKKFCGEDLTLSAAGRTDAGVHATAQVAHVDLAKDWGAGKVRDAVN
AHLVMADERISILNVEKTTDTFDARFSARARHYLYRIHNRRAPLAVDYQRAWWVQKQLDADAMHEAAQRLLGEHDFTTFR
ATQCQAKSPVKTLDRLDVTRNGDMVEMRVSARSFLHNQVRSFAGSLMEVGVGRWTADDLQAALEARDRKACGQVAPPYGL
YLVGVDYAFPF

Sequences:

>Translated_251_residues
MPRYKLTVEYDGTPYVGWQRQENGHAVQGAIEQAFKKFCGEDLTLSAAGRTDAGVHATAQVAHVDLAKDWGAGKVRDAVN
AHLVMADERISILNVEKTTDTFDARFSARARHYLYRIHNRRAPLAVDYQRAWWVQKQLDADAMHEAAQRLLGEHDFTTFR
ATQCQAKSPVKTLDRLDVTRNGDMVEMRVSARSFLHNQVRSFAGSLMEVGVGRWTADDLQAALEARDRKACGQVAPPYGL
YLVGVDYAFPF
>Mature_250_residues
PRYKLTVEYDGTPYVGWQRQENGHAVQGAIEQAFKKFCGEDLTLSAAGRTDAGVHATAQVAHVDLAKDWGAGKVRDAVNA
HLVMADERISILNVEKTTDTFDARFSARARHYLYRIHNRRAPLAVDYQRAWWVQKQLDADAMHEAAQRLLGEHDFTTFRA
TQCQAKSPVKTLDRLDVTRNGDMVEMRVSARSFLHNQVRSFAGSLMEVGVGRWTADDLQAALEARDRKACGQVAPPYGLY
LVGVDYAFPF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=32.1167883211679, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788657, Length=253, Percent_Identity=44.2687747035573, Blast_Score=185, Evalue=3e-48,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=26.158940397351, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BRUA2 (Q2YJQ4)

Other databases:

- EMBL:   AM040265
- RefSeq:   YP_419130.1
- ProteinModelPortal:   Q2YJQ4
- SMR:   Q2YJQ4
- STRING:   Q2YJQ4
- GeneID:   3827420
- GenomeReviews:   AM040265_GR
- KEGG:   bmf:BAB2_0995
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   RTYLYRV
- ProtClustDB:   PRK00021
- BioCyc:   BMEL359391:BAB2_0995-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28172; Mature: 28041

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYKLTVEYDGTPYVGWQRQENGHAVQGAIEQAFKKFCGEDLTLSAAGRTDAGVHATAQ
CCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEH
VAHVDLAKDWGAGKVRDAVNAHLVMADERISILNVEKTTDTFDARFSARARHYLYRIHNR
EEEEEHHCCCCCCHHHHHCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
RAPLAVDYQRAWWVQKQLDADAMHEAAQRLLGEHDFTTFRATQCQAKSPVKTLDRLDVTR
CCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCC
NGDMVEMRVSARSFLHNQVRSFAGSLMEVGVGRWTADDLQAALEARDRKACGQVAPPYGL
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCE
YLVGVDYAFPF
EEEEECCCCCC
>Mature Secondary Structure 
PRYKLTVEYDGTPYVGWQRQENGHAVQGAIEQAFKKFCGEDLTLSAAGRTDAGVHATAQ
CCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEH
VAHVDLAKDWGAGKVRDAVNAHLVMADERISILNVEKTTDTFDARFSARARHYLYRIHNR
EEEEEHHCCCCCCHHHHHCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
RAPLAVDYQRAWWVQKQLDADAMHEAAQRLLGEHDFTTFRATQCQAKSPVKTLDRLDVTR
CCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCC
NGDMVEMRVSARSFLHNQVRSFAGSLMEVGVGRWTADDLQAALEARDRKACGQVAPPYGL
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCE
YLVGVDYAFPF
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA