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The map label for this gene is nylB' [H]

Identifier: 163845089

GI number: 163845089

Start: 951542

End: 952759

Strand: Reverse

Name: nylB' [H]

Synonym: BSUIS_B0969

Alternate gene names: 163845089

Gene position: 952759-951542 (Counterclockwise)

Preceding gene: 163845090

Following gene: 163845088

Centisome position: 68.01

GC content: 52.3

Gene sequence:

>1218_bases
ATGAAGCGTCTATTCCGCTTTGCCTTATATCTGTGTGTTGCCCTCATTATCTTTGCAGGGGCTGGAACAGTAATCTTCTG
GAAGAAGATAAATGAATACTGGGCGGTGTATCAGTATGCGCAGGTGTTCAAGCCCGATACCATAAATGAGAATTTCCGCA
GCCTTTACAAGAAATATCCCTCGGTTCGTGTGCCGCATACGGGCGAGGTCAGCCCCTTGCCGAGAGGTGAACAGCCGCTT
CCCGAAACCTATACCTACAAGGGCGAGACCCGTAAGGTTGCCGACTGGCTGGAGCGGACCGACACGACCGGCTTTATCGT
GCTGAAAGATGGCGTCGTTGTTCACGAGCAATATGAGCGCGGCAACAGCGAAACAACGCAATCGATTGCAATGTCGCTGT
CGAAATCCTTCGTTTCATTCCTTGTCGGCAATGCGGTGCGTGACGGTCAGATCAAACTTGACCAGACGGTGGATCATTAT
GCGCCTTTGCTGAAGGAGGGCGGTTACAAGGAGGTGACGGTCAAGAACGTCCTGCAAATGTCGTCGGGCATCGGCTTCAA
TGAAAACTATGGCGATCTCAATTCGGATATCGTGCGCTATATCGTGCAGATGCTGACGGGATCAGTCGATGATTTTACCG
CCAAACTGAAGAACGAAGTCCCGCAAGGGACCATCAATCATTATGTGTCTGCTGATACCCAGGTTCTGGCCATGGTGCTG
GAAGGGGCGACGGGCGTTCCATTCCAGAAATATTTTGAAGACAAGCTTTGGTCGAGGCTTGGCGCCGAAGCCGATGCTTA
TTGGCTGACCGACGAGAAGGGTGACGTCGTGGCGGCGGGCGGACTTAATGCGGTGCTTCGCGACTATGCGCGCTTTGGCC
TTCTCTATATGAATGAAGGACGCAACTATCGCGGTGAGCAGCTTGTGCCGGCACAATGGGTGCATGATTCAGTAACGCCG
GACCAGCCTTACCTCATGCCCGGCCGCAAGGATGCCTTGGGGAAAATTCCCTTCGGCTATGGCTATCAATGGTGGATTCC
TGTCGAACCGGAAGGAGATTTCTGTGCGGTGGGCATCTACGGTCAGTTCATTTATGTCAATCCGGCCAAGCATGTCGTGA
TCGCCAAAACCTCAGCCTATCCGAATTATAATATTGACGGGCAGAAGGTGAAGGACGAATCCATGTATGTTTTCCAAGCC
ATCGCGAATGCGCTTTAG

Upstream 100 bases:

>100_bases
TTTTTGTTAATATATATCCGAATGATTGTGTATTTTTATTATTTTAAATAACTAATACATGATTTTCTTATTTAGGTATC
AAGTATATAAAGGGGAGGAA

Downstream 100 bases:

>100_bases
ATAAGTGCGGCAGGTCCGGTGTGAGGACAAAAAACTATTGGAGCAATCGCCTGATGGTTCCGTTCCAACTCACCGGAAAA
TGTAAAAATGGCCGCAATCT

Product: hypothetical protein

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII' [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKRLFRFALYLCVALIIFAGAGTVIFWKKINEYWAVYQYAQVFKPDTINENFRSLYKKYPSVRVPHTGEVSPLPRGEQPL
PETYTYKGETRKVADWLERTDTTGFIVLKDGVVVHEQYERGNSETTQSIAMSLSKSFVSFLVGNAVRDGQIKLDQTVDHY
APLLKEGGYKEVTVKNVLQMSSGIGFNENYGDLNSDIVRYIVQMLTGSVDDFTAKLKNEVPQGTINHYVSADTQVLAMVL
EGATGVPFQKYFEDKLWSRLGAEADAYWLTDEKGDVVAAGGLNAVLRDYARFGLLYMNEGRNYRGEQLVPAQWVHDSVTP
DQPYLMPGRKDALGKIPFGYGYQWWIPVEPEGDFCAVGIYGQFIYVNPAKHVVIAKTSAYPNYNIDGQKVKDESMYVFQA
IANAL

Sequences:

>Translated_405_residues
MKRLFRFALYLCVALIIFAGAGTVIFWKKINEYWAVYQYAQVFKPDTINENFRSLYKKYPSVRVPHTGEVSPLPRGEQPL
PETYTYKGETRKVADWLERTDTTGFIVLKDGVVVHEQYERGNSETTQSIAMSLSKSFVSFLVGNAVRDGQIKLDQTVDHY
APLLKEGGYKEVTVKNVLQMSSGIGFNENYGDLNSDIVRYIVQMLTGSVDDFTAKLKNEVPQGTINHYVSADTQVLAMVL
EGATGVPFQKYFEDKLWSRLGAEADAYWLTDEKGDVVAAGGLNAVLRDYARFGLLYMNEGRNYRGEQLVPAQWVHDSVTP
DQPYLMPGRKDALGKIPFGYGYQWWIPVEPEGDFCAVGIYGQFIYVNPAKHVVIAKTSAYPNYNIDGQKVKDESMYVFQA
IANAL
>Mature_405_residues
MKRLFRFALYLCVALIIFAGAGTVIFWKKINEYWAVYQYAQVFKPDTINENFRSLYKKYPSVRVPHTGEVSPLPRGEQPL
PETYTYKGETRKVADWLERTDTTGFIVLKDGVVVHEQYERGNSETTQSIAMSLSKSFVSFLVGNAVRDGQIKLDQTVDHY
APLLKEGGYKEVTVKNVLQMSSGIGFNENYGDLNSDIVRYIVQMLTGSVDDFTAKLKNEVPQGTINHYVSADTQVLAMVL
EGATGVPFQKYFEDKLWSRLGAEADAYWLTDEKGDVVAAGGLNAVLRDYARFGLLYMNEGRNYRGEQLVPAQWVHDSVTP
DQPYLMPGRKDALGKIPFGYGYQWWIPVEPEGDFCAVGIYGQFIYVNPAKHVVIAKTSAYPNYNIDGQKVKDESMYVFQA
IANAL

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 45681; Mature: 45681

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFRFALYLCVALIIFAGAGTVIFWKKINEYWAVYQYAQVFKPDTINENFRSLYKKYP
CHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
SVRVPHTGEVSPLPRGEQPLPETYTYKGETRKVADWLERTDTTGFIVLKDGVVVHEQYER
CCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCEEEEEECCEEEEHHHHC
GNSETTQSIAMSLSKSFVSFLVGNAVRDGQIKLDQTVDHYAPLLKEGGYKEVTVKNVLQM
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHH
SSGIGFNENYGDLNSDIVRYIVQMLTGSVDDFTAKLKNEVPQGTINHYVSADTQVLAMVL
HCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHH
EGATGVPFQKYFEDKLWSRLGAEADAYWLTDEKGDVVAAGGLNAVLRDYARFGLLYMNEG
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHCEEEEECC
RNYRGEQLVPAQWVHDSVTPDQPYLMPGRKDALGKIPFGYGYQWWIPVEPEGDFCAVGIY
CCCCCCCCCCHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEE
GQFIYVNPAKHVVIAKTSAYPNYNIDGQKVKDESMYVFQAIANAL
EEEEEECCCCEEEEEEECCCCCCCCCCCEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MKRLFRFALYLCVALIIFAGAGTVIFWKKINEYWAVYQYAQVFKPDTINENFRSLYKKYP
CHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
SVRVPHTGEVSPLPRGEQPLPETYTYKGETRKVADWLERTDTTGFIVLKDGVVVHEQYER
CCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCEEEEEECCEEEEHHHHC
GNSETTQSIAMSLSKSFVSFLVGNAVRDGQIKLDQTVDHYAPLLKEGGYKEVTVKNVLQM
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHH
SSGIGFNENYGDLNSDIVRYIVQMLTGSVDDFTAKLKNEVPQGTINHYVSADTQVLAMVL
HCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHH
EGATGVPFQKYFEDKLWSRLGAEADAYWLTDEKGDVVAAGGLNAVLRDYARFGLLYMNEG
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHCEEEEECC
RNYRGEQLVPAQWVHDSVTPDQPYLMPGRKDALGKIPFGYGYQWWIPVEPEGDFCAVGIY
CCCCCCCCCCHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEE
GQFIYVNPAKHVVIAKTSAYPNYNIDGQKVKDESMYVFQAIANAL
EEEEEECCCCEEEEEEECCCCCCCCCCCEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 1879421 [H]