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The map label for this gene is purU [H]

Identifier: 163845026

GI number: 163845026

Start: 879656

End: 880540

Strand: Reverse

Name: purU [H]

Synonym: BSUIS_B0902

Alternate gene names: 163845026

Gene position: 880540-879656 (Counterclockwise)

Preceding gene: 163845027

Following gene: 163845023

Centisome position: 62.86

GC content: 55.25

Gene sequence:

>885_bases
ATGCTTAACTTTGTCCTGACTGTCACTTGCAAGTCCACCCGCGGTATCGTTGCCGCTATTTCGGGTTATCTTGCCGGCAA
GGGTTGCAACATCATCGATAGCGCCCAATTCGACGATCTGGATACGGGGCGCTTTTTCATGCGCGTCAGCTTCATTTCCG
AGGAGGGCGTGCTTCTGGATGAATTGTGCGATGGTTTTGCCGCAGTTGCCGCACCGTTCGAGATGAATTTCAATTTTTAT
GACAATGCCCATCGCGTCAAGACGCTTTTGATGGTGTCGCGCTTCGGCCATTGCCTCAACGATCTTCTCTATCGCTGGAA
GATCGGCGCGCTGCCCATCGATATCGTGGGCGTGGTGTCAAACCATTTCGACTATCAAAAGGTCGTTGTGAACCACGATA
TTCCCTTCCATCATATCGCGGTCACCAAGGCCAACAAGCCGGAAGCCGAGCGCCATTTGATGGAAATCGTGGAAGATACC
AACACGGAACTGGTGGTGCTTGCGCGCTATATGCAGGTTCTCTCCGACGAATTTTGCCAGAAAATGTCGGGCCGCATCAT
CAATATCCACCATTCCTTCCTGCCGTCCTTCAAAGGCGCGAACCCTTACAAGCAGGCCTATGAGCGCGGCGTGAAACTTA
TTGGCGCGACGGCCCATTATGTCACCGCCAATCTCGATGAAGGCCCGATCATCGAACAGGACGTGGCACGCATCACCCAT
GCGCAGAACTCGGCCGATTATGTGTCCATCGGGCGCGATGTCGAGGCGCAGGTTCTGGCGCGCGCTGTTCACGCGCACAT
CCATCACCGCTCATTCCTCAACGGCAACCGCACCGTCGTATTCCCGGCTAGCCCCGGCTCGTTCGCCTCCGAACGCATGG
GGTGA

Upstream 100 bases:

>100_bases
TCCGGTCAGTGCATTACGCATTCTGGCAAGGCTGAAGCATTGCGCTGGATTAGGCTCGCAAAAGCGTTTTGGCTGATGCC
CTGGAAATAGAGGAAGACTG

Downstream 100 bases:

>100_bases
TATTGCGGCTCTGGCGGCCTGCAAATCGCGTCTTTTTACGCTTCCGGTGCTCACGTACTCAAGTACGGCTCAAGCGCCGG
TTCGCAAAAGCCACGATTTT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLNFVLTVTCKSTRGIVAAISGYLAGKGCNIIDSAQFDDLDTGRFFMRVSFISEEGVLLDELCDGFAAVAAPFEMNFNFY
DNAHRVKTLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHHIAVTKANKPEAERHLMEIVEDT
NTELVVLARYMQVLSDEFCQKMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTANLDEGPIIEQDVARITH
AQNSADYVSIGRDVEAQVLARAVHAHIHHRSFLNGNRTVVFPASPGSFASERMG

Sequences:

>Translated_294_residues
MLNFVLTVTCKSTRGIVAAISGYLAGKGCNIIDSAQFDDLDTGRFFMRVSFISEEGVLLDELCDGFAAVAAPFEMNFNFY
DNAHRVKTLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHHIAVTKANKPEAERHLMEIVEDT
NTELVVLARYMQVLSDEFCQKMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTANLDEGPIIEQDVARITH
AQNSADYVSIGRDVEAQVLARAVHAHIHHRSFLNGNRTVVFPASPGSFASERMG
>Mature_294_residues
MLNFVLTVTCKSTRGIVAAISGYLAGKGCNIIDSAQFDDLDTGRFFMRVSFISEEGVLLDELCDGFAAVAAPFEMNFNFY
DNAHRVKTLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHHIAVTKANKPEAERHLMEIVEDT
NTELVVLARYMQVLSDEFCQKMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTANLDEGPIIEQDVARITH
AQNSADYVSIGRDVEAQVLARAVHAHIHHRSFLNGNRTVVFPASPGSFASERMG

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=101, Percent_Identity=38.6138613861386, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI209869995, Length=101, Percent_Identity=38.6138613861386, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI209869993, Length=101, Percent_Identity=38.6138613861386, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=40.7942238267148, Blast_Score=213, Evalue=8e-57,
Organism=Escherichia coli, GI1788846, Length=84, Percent_Identity=39.2857142857143, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=151, Percent_Identity=36.4238410596026, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24582400, Length=82, Percent_Identity=40.2439024390244, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32768; Mature: 32768

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFVLTVTCKSTRGIVAAISGYLAGKGCNIIDSAQFDDLDTGRFFMRVSFISEEGVLLD
CCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEHH
ELCDGFAAVAAPFEMNFNFYDNAHRVKTLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVS
HHHCCHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
NHFDYQKVVVNHDIPFHHIAVTKANKPEAERHLMEIVEDTNTELVVLARYMQVLSDEFCQ
CCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTANLDEGPIIEQDVARITH
HHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECEEEEEEECCCCCCCCHHHHHHHHH
AQNSADYVSIGRDVEAQVLARAVHAHIHHRSFLNGNRTVVFPASPGSFASERMG
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCC
>Mature Secondary Structure
MLNFVLTVTCKSTRGIVAAISGYLAGKGCNIIDSAQFDDLDTGRFFMRVSFISEEGVLLD
CCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEHH
ELCDGFAAVAAPFEMNFNFYDNAHRVKTLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVS
HHHCCHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
NHFDYQKVVVNHDIPFHHIAVTKANKPEAERHLMEIVEDTNTELVVLARYMQVLSDEFCQ
CCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KMSGRIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTANLDEGPIIEQDVARITH
HHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECEEEEEEECCCCCCCCHHHHHHHHH
AQNSADYVSIGRDVEAQVLARAVHAHIHHRSFLNGNRTVVFPASPGSFASERMG
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]