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The map label for this gene is folD

Identifier: 163844908

GI number: 163844908

Start: 749780

End: 750679

Strand: Reverse

Name: folD

Synonym: BSUIS_B0773

Alternate gene names: 163844908

Gene position: 750679-749780 (Counterclockwise)

Preceding gene: 163844910

Following gene: 163844905

Centisome position: 53.59

GC content: 59.11

Gene sequence:

>900_bases
ATGGCCCAATTGATCGATGGCAAGAAGCTCGCCGAGGATGTGGTTTCCACGGTGAAAACCGAAACCGAAAAACTTGTGGC
AGCGACCGGCGTCGTTCCCGGTATTGCCGTGGTGATTGTCGGTGAAGACCCGGCAAGTCAGGTCTATGTTGCCTCGAAAA
GCCGCAAGGCCAAGGAATGCGGCTTTCATTCCGTTCAGCACGACTTGCCGGAAACGGCTTCCGAACAAGAGCTTCTCAAC
CTGATCGAAGGCCTCAATAACGACCCCGCCATTCATGGCATTCTGGTTCAGTTGCCTTTGCCGGGGCACATTGATTCAGG
CCGCGTGATCCAGACCATCGCTCCTGAAAAGGACGTGGACGGCTTCCACTTCATCAATGTGGGCAAGCTTGGCACCGGCG
AGGTGGAAACGGCTTTCGTTCCCTGCACGCCAGCCGGCGCGATGATTATGATCGAGCGTGTGCATGGCCGCGACCTTTCC
GGCCTCAATGCCGTGGTGATCGGGCGTTCCAATATTGTCGGCAAGCCGATGTTCAACCTCCTTCTGGCGGCAAATGCGAC
GGTGACGGTTGCGCATAGCCGCACCAAGGACCTGCCCGCGATTGCGCGCAATGCCGATATTCTGGTGGCGGCGGTGGGGC
GTCCGCAAATGGTCAAGGGAGATTGGGTCAAGCCGGGCGCGACGGTGATCGATGTGGGTATCAACCGCATCCCGGCACCG
GAGCGCGGCGAGGGCAAGACAAGGCTCGTCGGTGATGTCGATTTTGCCGAGGCTGAAAAGGTTGCAGGCGCGATCACGCC
GGTTCCGGGCGGGGTTGGCCCAATGACCATCGCCATGCTGATGGCCAATACGCTCACCGCTGCCTGCCGCAGTGCAGGCA
TGAAAAAACCCGTTTTCTGA

Upstream 100 bases:

>100_bases
TTGAACGATTGTCATACGGCCCCATGACGCTTATGGGCATATGTGCGCAAACGACGTGATGCAGGATGCTGCATCATCTG
CTGAAAACGGAGCGATCACG

Downstream 100 bases:

>100_bases
TGCGGACGAGCCGCGCTTTATTCCGGCTTTGATGGTTTGAAAGGCAAAACAGCCGAAATTGCTGTTTTGCCTTCATTTTA
TTCGCGCTGTTTCAGAACAC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MAQLIDGKKLAEDVVSTVKTETEKLVAATGVVPGIAVVIVGEDPASQVYVASKSRKAKECGFHSVQHDLPETASEQELLN
LIEGLNNDPAIHGILVQLPLPGHIDSGRVIQTIAPEKDVDGFHFINVGKLGTGEVETAFVPCTPAGAMIMIERVHGRDLS
GLNAVVIGRSNIVGKPMFNLLLAANATVTVAHSRTKDLPAIARNADILVAAVGRPQMVKGDWVKPGATVIDVGINRIPAP
ERGEGKTRLVGDVDFAEAEKVAGAITPVPGGVGPMTIAMLMANTLTAACRSAGMKKPVF

Sequences:

>Translated_299_residues
MAQLIDGKKLAEDVVSTVKTETEKLVAATGVVPGIAVVIVGEDPASQVYVASKSRKAKECGFHSVQHDLPETASEQELLN
LIEGLNNDPAIHGILVQLPLPGHIDSGRVIQTIAPEKDVDGFHFINVGKLGTGEVETAFVPCTPAGAMIMIERVHGRDLS
GLNAVVIGRSNIVGKPMFNLLLAANATVTVAHSRTKDLPAIARNADILVAAVGRPQMVKGDWVKPGATVIDVGINRIPAP
ERGEGKTRLVGDVDFAEAEKVAGAITPVPGGVGPMTIAMLMANTLTAACRSAGMKKPVF
>Mature_298_residues
AQLIDGKKLAEDVVSTVKTETEKLVAATGVVPGIAVVIVGEDPASQVYVASKSRKAKECGFHSVQHDLPETASEQELLNL
IEGLNNDPAIHGILVQLPLPGHIDSGRVIQTIAPEKDVDGFHFINVGKLGTGEVETAFVPCTPAGAMIMIERVHGRDLSG
LNAVVIGRSNIVGKPMFNLLLAANATVTVAHSRTKDLPAIARNADILVAAVGRPQMVKGDWVKPGATVIDVGINRIPAPE
RGEGKTRLVGDVDFAEAEKVAGAITPVPGGVGPMTIAMLMANTLTAACRSAGMKKPVF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=299, Percent_Identity=45.1505016722408, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=44.1077441077441, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=46.4646464646465, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=27.6381909547739, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1786741, Length=287, Percent_Identity=54.006968641115, Blast_Score=278, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17568735, Length=260, Percent_Identity=46.1538461538462, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=44.4827586206897, Blast_Score=249, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=44.3333333333333, Blast_Score=243, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322933, Length=309, Percent_Identity=25.5663430420712, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=48.2876712328767, Blast_Score=260, Evalue=7e-70,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=48.2876712328767, Blast_Score=260, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=48.2876712328767, Blast_Score=260, Evalue=7e-70,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=48.2876712328767, Blast_Score=260, Evalue=7e-70,
Organism=Drosophila melanogaster, GI17136816, Length=306, Percent_Identity=47.7124183006536, Blast_Score=245, Evalue=2e-65,
Organism=Drosophila melanogaster, GI17136818, Length=306, Percent_Identity=47.7124183006536, Blast_Score=245, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_BRUA1 (B2SAP4)

Other databases:

- EMBL:   CP000888
- RefSeq:   YP_001932377.1
- ProteinModelPortal:   B2SAP4
- SMR:   B2SAP4
- GeneID:   6326166
- GenomeReviews:   CP000888_GR
- KEGG:   bmc:BAbS19_II04320
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- ProtClustDB:   PRK14188
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31232; Mature: 31100

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLIDGKKLAEDVVSTVKTETEKLVAATGVVPGIAVVIVGEDPASQVYVASKSRKAKEC
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCHHHC
GFHSVQHDLPETASEQELLNLIEGLNNDPAIHGILVQLPLPGHIDSGRVIQTIAPEKDVD
CCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCC
GFHFINVGKLGTGEVETAFVPCTPAGAMIMIERVHGRDLSGLNAVVIGRSNIVGKPMFNL
CEEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHH
LLAANATVTVAHSRTKDLPAIARNADILVAAVGRPQMVKGDWVKPGATVIDVGINRIPAP
HHHCCCEEEEEECCCCCCCHHHCCCCEEEEECCCCCEECCCCCCCCCEEEEECCCCCCCC
ERGEGKTRLVGDVDFAEAEKVAGAITPVPGGVGPMTIAMLMANTLTAACRSAGMKKPVF
CCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AQLIDGKKLAEDVVSTVKTETEKLVAATGVVPGIAVVIVGEDPASQVYVASKSRKAKEC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCHHHC
GFHSVQHDLPETASEQELLNLIEGLNNDPAIHGILVQLPLPGHIDSGRVIQTIAPEKDVD
CCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCC
GFHFINVGKLGTGEVETAFVPCTPAGAMIMIERVHGRDLSGLNAVVIGRSNIVGKPMFNL
CEEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHH
LLAANATVTVAHSRTKDLPAIARNADILVAAVGRPQMVKGDWVKPGATVIDVGINRIPAP
HHHCCCEEEEEECCCCCCCHHHCCCCEEEEECCCCCEECCCCCCCCCEEEEECCCCCCCC
ERGEGKTRLVGDVDFAEAEKVAGAITPVPGGVGPMTIAMLMANTLTAACRSAGMKKPVF
CCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA