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The map label for this gene is proW [H]

Identifier: 163844870

GI number: 163844870

Start: 710747

End: 711652

Strand: Reverse

Name: proW [H]

Synonym: BSUIS_B0731

Alternate gene names: 163844870

Gene position: 711652-710747 (Counterclockwise)

Preceding gene: 163844871

Following gene: 163844869

Centisome position: 50.8

GC content: 59.6

Gene sequence:

>906_bases
ATGGATTTGAATTTCAGTGTCGGCGGATGGGCGGATGTGGCGGTCAATTACATACTCGACCACTTCACGCCCGCGCTCGA
TATGATTGCGGCTGCGATCGGCTTTGTAACGGATGGCATCCAGAATGCTCTCATCGCCGTTCCGCCCTTTGCGGGCGTTG
CTATCCTCAGCTTGCTGGCGCTTTGGCGCGTCGGCTGGAAATTTGCGGTTTTCACGCTTCTGGCGCTCGCCCTCATCATC
CACATGGATCTGTGGAGCGGCACGATGGAAAGCTTGTCGCTGGTGCTGGCATCAACCGTCATTGCGGTGGTGATCGGCAT
TCCGCTTGGTATCGCCATGGCGCGCAGTGACGCTGTTGCCTCAATCGTAAGGCCGATCCTCGACCTCATGCAGACGATGC
CGGCTTTCGTCTATCTTATTCCGGCGGCAATGTTTTTCGGTCTTGGCGCGGTGCCGGGCACAATTGCCACCGTGATCTTC
GCCATGCCGCCCGTGGTGCGTCTCACCAATCTCGGCATCCGGCAGGTTCATGCGGAATTTGTGGAGGCGGGCCTCGCTTT
CGGCTGCACCGCGCGGCAGCTTCTGTTCAAGGTCCAGCTTCCTAACGCCATGCCGTCCATCATGGCAGGTGTCAACCAGA
CGATCATGCTGTCGCTTTCCATGGTGGTCATCGCCTCGATGATCGGCGCGGGCGGCCTTGGCAACACGGTGCTGACCGGC
ATCCAGCGACTCAATGTGGGCCTTGGCTTCGAGGGCGGTCTGGCGGTTGTCATTCTGGCGGTCATTCTCGATCGCATCAC
GCAAAGCTTCGGCAAGGGCGGCACGGGCCTGCTCAAGCGGCTCGCATTCTTGAAAAGGGCGGCAGGCGAACAGCCTGCCT
CATCCTTTCGCAAGCAGGAAGCATGA

Upstream 100 bases:

>100_bases
GGTCGGTCTTGCTTGAAACGCTCGGGCGCGCGGGCTGAAGCCCGCACGCTGGCGACCTTTGGGTCCAATCCAGAAACAAG
ACGGAAATGGACGGTAAATC

Downstream 100 bases:

>100_bases
AACAAACATCAATAACGGGAACTGAAACCAAAGGAGAAGGAGAAGGAGAAGGTATGTTGGGCAAACTAACCAGATTCGGT
CTGGCGGCCGTTGTGGCGGG

Product: hypothetical protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDLNFSVGGWADVAVNYILDHFTPALDMIAAAIGFVTDGIQNALIAVPPFAGVAILSLLALWRVGWKFAVFTLLALALII
HMDLWSGTMESLSLVLASTVIAVVIGIPLGIAMARSDAVASIVRPILDLMQTMPAFVYLIPAAMFFGLGAVPGTIATVIF
AMPPVVRLTNLGIRQVHAEFVEAGLAFGCTARQLLFKVQLPNAMPSIMAGVNQTIMLSLSMVVIASMIGAGGLGNTVLTG
IQRLNVGLGFEGGLAVVILAVILDRITQSFGKGGTGLLKRLAFLKRAAGEQPASSFRKQEA

Sequences:

>Translated_301_residues
MDLNFSVGGWADVAVNYILDHFTPALDMIAAAIGFVTDGIQNALIAVPPFAGVAILSLLALWRVGWKFAVFTLLALALII
HMDLWSGTMESLSLVLASTVIAVVIGIPLGIAMARSDAVASIVRPILDLMQTMPAFVYLIPAAMFFGLGAVPGTIATVIF
AMPPVVRLTNLGIRQVHAEFVEAGLAFGCTARQLLFKVQLPNAMPSIMAGVNQTIMLSLSMVVIASMIGAGGLGNTVLTG
IQRLNVGLGFEGGLAVVILAVILDRITQSFGKGGTGLLKRLAFLKRAAGEQPASSFRKQEA
>Mature_301_residues
MDLNFSVGGWADVAVNYILDHFTPALDMIAAAIGFVTDGIQNALIAVPPFAGVAILSLLALWRVGWKFAVFTLLALALII
HMDLWSGTMESLSLVLASTVIAVVIGIPLGIAMARSDAVASIVRPILDLMQTMPAFVYLIPAAMFFGLGAVPGTIATVIF
AMPPVVRLTNLGIRQVHAEFVEAGLAFGCTARQLLFKVQLPNAMPSIMAGVNQTIMLSLSMVVIASMIGAGGLGNTVLTG
IQRLNVGLGFEGGLAVVILAVILDRITQSFGKGGTGLLKRLAFLKRAAGEQPASSFRKQEA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=263, Percent_Identity=50.1901140684411, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=32.4607329842932, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788449, Length=175, Percent_Identity=31.4285714285714, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786564, Length=159, Percent_Identity=32.0754716981132, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31588; Mature: 31588

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNFSVGGWADVAVNYILDHFTPALDMIAAAIGFVTDGIQNALIAVPPFAGVAILSLLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHH
LWRVGWKFAVFTLLALALIIHMDLWSGTMESLSLVLASTVIAVVIGIPLGIAMARSDAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIVRPILDLMQTMPAFVYLIPAAMFFGLGAVPGTIATVIFAMPPVVRLTNLGIRQVHAEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VEAGLAFGCTARQLLFKVQLPNAMPSIMAGVNQTIMLSLSMVVIASMIGAGGLGNTVLTG
HHHHHHHCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IQRLNVGLGFEGGLAVVILAVILDRITQSFGKGGTGLLKRLAFLKRAAGEQPASSFRKQE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCC
A
C
>Mature Secondary Structure
MDLNFSVGGWADVAVNYILDHFTPALDMIAAAIGFVTDGIQNALIAVPPFAGVAILSLLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHH
LWRVGWKFAVFTLLALALIIHMDLWSGTMESLSLVLASTVIAVVIGIPLGIAMARSDAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIVRPILDLMQTMPAFVYLIPAAMFFGLGAVPGTIATVIFAMPPVVRLTNLGIRQVHAEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VEAGLAFGCTARQLLFKVQLPNAMPSIMAGVNQTIMLSLSMVVIASMIGAGGLGNTVLTG
HHHHHHHCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IQRLNVGLGFEGGLAVVILAVILDRITQSFGKGGTGLLKRLAFLKRAAGEQPASSFRKQE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]