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The map label for this gene is gcvH

Identifier: 163844857

GI number: 163844857

Start: 699590

End: 699958

Strand: Reverse

Name: gcvH

Synonym: BSUIS_B0716

Alternate gene names: 163844857

Gene position: 699958-699590 (Counterclockwise)

Preceding gene: 163844858

Following gene: 163844856

Centisome position: 49.97

GC content: 58.81

Gene sequence:

>369_bases
ATGGCCAATATCCTGTTCACCGAAGATCATGAGTGGATCAACGTCGAAAACGGCGTTGCCACGGTTGGCATCACGATCCA
CGCACAGGAACAGCTCGGCGACCTCGTTTTCGTCGAATTGCCGGAAGTGGGCCGCACCGTTGCCAAGGGCGATGGCGTGG
TGGTTGTGGAGTCGGTCAAGGCCGCTTCCGACGTCTACGCGCCGGTCGATGGCGAAGTGGTGGAAGTCAACGATGCTGTC
GCGAGCGATCCTTCCCTTATCAATCAGGCTGCCGAAGGCGAAGGCTGGCTGTTCAAGCTGAAGCTTGCCGATGAAGGCCA
GCTCACGGGCCTGCTCGACAAGGCCGGTTATGAAAAGCTGATCGGGTGA

Upstream 100 bases:

>100_bases
CCGGCACCCGTGTTTTTGCAGATGTTCGCGGCAACAAGGTTCCCGTTGACGTCCACGCACTTCCCTTCACACCGCATCGC
TATCGCAAAGGATGATTTCC

Downstream 100 bases:

>100_bases
TCGAATGACCGAATTTCTTCCCTTTGTGGCCCGCCATATCGGGCCGAGACATGAAGACGAGCGCGCCATGCTGGCAGCGC
TCGGCCTTCCTTCCATGGAA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 121

Protein sequence:

>122_residues
MANILFTEDHEWINVENGVATVGITIHAQEQLGDLVFVELPEVGRTVAKGDGVVVVESVKAASDVYAPVDGEVVEVNDAV
ASDPSLINQAAEGEGWLFKLKLADEGQLTGLLDKAGYEKLIG

Sequences:

>Translated_122_residues
MANILFTEDHEWINVENGVATVGITIHAQEQLGDLVFVELPEVGRTVAKGDGVVVVESVKAASDVYAPVDGEVVEVNDAV
ASDPSLINQAAEGEGWLFKLKLADEGQLTGLLDKAGYEKLIG
>Mature_121_residues
ANILFTEDHEWINVENGVATVGITIHAQEQLGDLVFVELPEVGRTVAKGDGVVVVESVKAASDVYAPVDGEVVEVNDAVA
SDPSLINQAAEGEGWLFKLKLADEGQLTGLLDKAGYEKLIG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=49.1379310344828, Blast_Score=126, Evalue=4e-30,
Organism=Homo sapiens, GI89057342, Length=116, Percent_Identity=49.1379310344828, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=55.3719008264463, Blast_Score=139, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=50, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=50.8620689655172, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319272, Length=123, Percent_Identity=43.0894308943089, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=43.2203389830509, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_BRUA2 (Q2YKY0)

Other databases:

- EMBL:   AM040265
- RefSeq:   YP_418704.1
- ProteinModelPortal:   Q2YKY0
- SMR:   Q2YKY0
- STRING:   Q2YKY0
- GeneID:   3828232
- GenomeReviews:   AM040265_GR
- KEGG:   bmf:BAB2_0514
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   BMEL359391:BAB2_0514-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 12919; Mature: 12787

Theoretical pI: Translated: 3.88; Mature: 3.88

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANILFTEDHEWINVENGVATVGITIHAQEQLGDLVFVELPEVGRTVAKGDGVVVVESVK
CCCEEEECCCCEEEECCCEEEEEEEEEEHHHCCCEEEEECCCCCCEEECCCCEEEEECCC
AASDVYAPVDGEVVEVNDAVASDPSLINQAAEGEGWLFKLKLADEGQLTGLLDKAGYEKL
CCCCEECCCCCCEEEECCCCCCCHHHHHHCCCCCCEEEEEEECCCCCEEEEHHHCCHHHH
IG
CC
>Mature Secondary Structure 
ANILFTEDHEWINVENGVATVGITIHAQEQLGDLVFVELPEVGRTVAKGDGVVVVESVK
CCEEEECCCCEEEECCCEEEEEEEEEEHHHCCCEEEEECCCCCCEEECCCCEEEEECCC
AASDVYAPVDGEVVEVNDAVASDPSLINQAAEGEGWLFKLKLADEGQLTGLLDKAGYEKL
CCCCEECCCCCCEEEECCCCCCCHHHHHHCCCCCCEEEEEEECCCCCEEEEHHHCCHHHH
IG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA