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The map label for this gene is hsrA [H]

Identifier: 163844816

GI number: 163844816

Start: 654113

End: 655522

Strand: Reverse

Name: hsrA [H]

Synonym: BSUIS_B0671

Alternate gene names: 163844816

Gene position: 655522-654113 (Counterclockwise)

Preceding gene: 163844817

Following gene: 163844809

Centisome position: 46.79

GC content: 57.73

Gene sequence:

>1410_bases
GTGAACCGCATTATTCCACTTGTGCTGGCGATTGCTCTTTTTATGGAGCAGATGGATTCGAATGTCATTTCGACCTCGCT
TCCGGCTATTGCCGCCGATATCGGCACCAGCCCAATTGCGCTGAAGCTGGCGCTCACCGCCTATCTTGTGGCGCTTGCGG
TTTTCATTCCGGTGAGCGGCTGGATGGCGGACAGGTTTGGCGCGAAGAACGTGTTTCGCGCAGCTATTGCGGTCTTTGTG
GTCGGCTCCATAGCATGTGCCGCTTCAAACTCGCTTCCGGCCTTTGTGATCGCCCGTTTCTTGCAGGGTATGGGCGGGGC
GATGATGACGCCGGTTGGCCGCCTGGTCCTCATCCGCTCCACGCCGCGAAACGAGCTTGTTTCGGCCATGGCGTGGCTTA
CCATTCCCGCCATGGTGGGGCCGCTTGTCGGCCCGCCGGTGGGTGGCTTCATAACGACCTTCCTCACTTGGCACTGGATA
TTCCTCATCAACGTGCCGATTGGCATGATCGGCATCTGGTTTGCCACGCATTTCCTGCCCGATAATGAAGAACGCATCAT
CAAGCGGCTCGATTGGCCGGGCTTCTTCCTCTCCGGTTTTGCCATGTCGGGCGTGGTGTTCGGCCTTTCTGTGGTCAGCC
TTCCGGCTTTGCCGCCCGTGATCGGCTTCGCCACGCTTGCTGTCGGCATGTTGTGCACTGTGCTTTATGTTCTCCACGCA
AGGCGTACGCCCGATCCGCTGCTCAATCTCAGGCTCTTCGACAATCAGGTTTTCCGGTCCGCGGTTTTCGGCGGCAGCAT
TTTCCGCTTCGGTATCGGTGCGATCCCGTTTCTGCTGCCCTTGATGTTCCAGCTCGGCTTTGGCCTCACGCCCTTTCAGT
CCGGCATGATGACCTTCGTTTCGGCAATCGGTGCGATTAGCATGAAATTCGGGGCAAAGCGCATTTTCACGCGCATCAGT
TTCCGCCGTGCACTTATGGGAGGATCGCTGATTTCAGCGGCCTTTATCGCGGTGAACGGCACGTTCACGCCTGCAACGCC
TTACTGGATCATCATTGGTTTCCTGCTTGTCGGCGGCTTTGCGCGGTCCCTGTTTTTTACCGGCGTAAATGCGTTGGCTT
TTGCTGAAATACCGAATGAGAAGACCAGTCAGGCAACGCCGCTTACCGCTGTCGCACAGCAGGTTTCGATTGCCATTGGC
GTGGCGCTTGCAGGCGGCATTCTGGAAATCAGCACCATGCTGCGCGGCGCACCGCTGGCCTTGGTTGATTTCCATATCGG
TTTCTTTGTGGTGGCCTGTGTTTCGGTGCTGGCTTTCTTCTGGTTTGCGCGGCTTGCGCCCGATGCAGGCAAGGAACTGG
CCACGCCAAGCGTGACACGCCATCATGGAAAACCCGTCACCACAGCCTAG

Upstream 100 bases:

>100_bases
TATCACCGCATTGTGACGCCTTGTCGACTAGCCAAACAAGTGAGGGATCCTACATAAATGCTACCATCTATTTTGGTGTC
CATTTTTCCGGAATATAACC

Downstream 100 bases:

>100_bases
TGGATTGAATTTGGCGTTTGAATCCCGGCTGCTACATATGCTGTTTCAAACGTCAAACTCTAAAAATCCACTAGATACAT
ATACTTACTAGTGGTTTTTG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MNRIIPLVLAIALFMEQMDSNVISTSLPAIAADIGTSPIALKLALTAYLVALAVFIPVSGWMADRFGAKNVFRAAIAVFV
VGSIACAASNSLPAFVIARFLQGMGGAMMTPVGRLVLIRSTPRNELVSAMAWLTIPAMVGPLVGPPVGGFITTFLTWHWI
FLINVPIGMIGIWFATHFLPDNEERIIKRLDWPGFFLSGFAMSGVVFGLSVVSLPALPPVIGFATLAVGMLCTVLYVLHA
RRTPDPLLNLRLFDNQVFRSAVFGGSIFRFGIGAIPFLLPLMFQLGFGLTPFQSGMMTFVSAIGAISMKFGAKRIFTRIS
FRRALMGGSLISAAFIAVNGTFTPATPYWIIIGFLLVGGFARSLFFTGVNALAFAEIPNEKTSQATPLTAVAQQVSIAIG
VALAGGILEISTMLRGAPLALVDFHIGFFVVACVSVLAFFWFARLAPDAGKELATPSVTRHHGKPVTTA

Sequences:

>Translated_469_residues
MNRIIPLVLAIALFMEQMDSNVISTSLPAIAADIGTSPIALKLALTAYLVALAVFIPVSGWMADRFGAKNVFRAAIAVFV
VGSIACAASNSLPAFVIARFLQGMGGAMMTPVGRLVLIRSTPRNELVSAMAWLTIPAMVGPLVGPPVGGFITTFLTWHWI
FLINVPIGMIGIWFATHFLPDNEERIIKRLDWPGFFLSGFAMSGVVFGLSVVSLPALPPVIGFATLAVGMLCTVLYVLHA
RRTPDPLLNLRLFDNQVFRSAVFGGSIFRFGIGAIPFLLPLMFQLGFGLTPFQSGMMTFVSAIGAISMKFGAKRIFTRIS
FRRALMGGSLISAAFIAVNGTFTPATPYWIIIGFLLVGGFARSLFFTGVNALAFAEIPNEKTSQATPLTAVAQQVSIAIG
VALAGGILEISTMLRGAPLALVDFHIGFFVVACVSVLAFFWFARLAPDAGKELATPSVTRHHGKPVTTA
>Mature_469_residues
MNRIIPLVLAIALFMEQMDSNVISTSLPAIAADIGTSPIALKLALTAYLVALAVFIPVSGWMADRFGAKNVFRAAIAVFV
VGSIACAASNSLPAFVIARFLQGMGGAMMTPVGRLVLIRSTPRNELVSAMAWLTIPAMVGPLVGPPVGGFITTFLTWHWI
FLINVPIGMIGIWFATHFLPDNEERIIKRLDWPGFFLSGFAMSGVVFGLSVVSLPALPPVIGFATLAVGMLCTVLYVLHA
RRTPDPLLNLRLFDNQVFRSAVFGGSIFRFGIGAIPFLLPLMFQLGFGLTPFQSGMMTFVSAIGAISMKFGAKRIFTRIS
FRRALMGGSLISAAFIAVNGTFTPATPYWIIIGFLLVGGFARSLFFTGVNALAFAEIPNEKTSQATPLTAVAQQVSIAIG
VALAGGILEISTMLRGAPLALVDFHIGFFVVACVSVLAFFWFARLAPDAGKELATPSVTRHHGKPVTTA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=455, Percent_Identity=39.1208791208791, Blast_Score=328, Evalue=6e-91,
Organism=Escherichia coli, GI1788392, Length=465, Percent_Identity=34.1935483870968, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87081983, Length=413, Percent_Identity=26.1501210653753, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788710, Length=413, Percent_Identity=24.9394673123487, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789042, Length=406, Percent_Identity=25.1231527093596, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=30.1470588235294, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790146, Length=186, Percent_Identity=25.8064516129032, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=32.2834645669291, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=410, Percent_Identity=24.390243902439, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50049; Mature: 50049

Theoretical pI: Translated: 11.12; Mature: 11.12

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIIPLVLAIALFMEQMDSNVISTSLPAIAADIGTSPIALKLALTAYLVALAVFIPVSG
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
WMADRFGAKNVFRAAIAVFVVGSIACAASNSLPAFVIARFLQGMGGAMMTPVGRLVLIRS
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHCCCCCEEEEEC
TPRNELVSAMAWLTIPAMVGPLVGPPVGGFITTFLTWHWIFLINVPIGMIGIWFATHFLP
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DNEERIIKRLDWPGFFLSGFAMSGVVFGLSVVSLPALPPVIGFATLAVGMLCTVLYVLHA
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
RRTPDPLLNLRLFDNQVFRSAVFGGSIFRFGIGAIPFLLPLMFQLGFGLTPFQSGMMTFV
CCCCCHHEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SAIGAISMKFGAKRIFTRISFRRALMGGSLISAAFIAVNGTFTPATPYWIIIGFLLVGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHH
ARSLFFTGVNALAFAEIPNEKTSQATPLTAVAQQVSIAIGVALAGGILEISTMLRGAPLA
HHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LVDFHIGFFVVACVSVLAFFWFARLAPDAGKELATPSVTRHHGKPVTTA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MNRIIPLVLAIALFMEQMDSNVISTSLPAIAADIGTSPIALKLALTAYLVALAVFIPVSG
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
WMADRFGAKNVFRAAIAVFVVGSIACAASNSLPAFVIARFLQGMGGAMMTPVGRLVLIRS
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHCCCCCEEEEEC
TPRNELVSAMAWLTIPAMVGPLVGPPVGGFITTFLTWHWIFLINVPIGMIGIWFATHFLP
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DNEERIIKRLDWPGFFLSGFAMSGVVFGLSVVSLPALPPVIGFATLAVGMLCTVLYVLHA
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
RRTPDPLLNLRLFDNQVFRSAVFGGSIFRFGIGAIPFLLPLMFQLGFGLTPFQSGMMTFV
CCCCCHHEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SAIGAISMKFGAKRIFTRISFRRALMGGSLISAAFIAVNGTFTPATPYWIIIGFLLVGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHH
ARSLFFTGVNALAFAEIPNEKTSQATPLTAVAQQVSIAIGVALAGGILEISTMLRGAPLA
HHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LVDFHIGFFVVACVSVLAFFWFARLAPDAGKELATPSVTRHHGKPVTTA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]