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The map label for this gene is glpK [H]

Identifier: 163844609

GI number: 163844609

Start: 431574

End: 433070

Strand: Reverse

Name: glpK [H]

Synonym: BSUIS_B0444

Alternate gene names: 163844609

Gene position: 433070-431574 (Counterclockwise)

Preceding gene: 163844610

Following gene: 163844608

Centisome position: 30.91

GC content: 59.39

Gene sequence:

>1497_bases
ATGAGCGGATATATTCTGGCTATCGATCAGGGAACCACCTCCACAAGGTCGATGCTGTTCGACAGGAATATGCGGGTCGT
GGGGTTGGGGCAGCAGGAATTTACGCAGCATTTTCCTTCCTCCGGCTGGGTGGAGCACGATGCGGAAGAAATCTGGAAAT
CGGTGCAGTCCACTATCCGCATAGCACTTGCACAAGCTGGCATCAGTGCGGCAGACGTCGCGGCGATCGGCATCACCAAT
CAGCGCAAAACAACCGTCGTATGGGACCGCATAAGCGGCAAGCCGGTACACCGCGCCATTGTCTGGCAGGATCGCCGCAC
GGCGCAGTTTTGCGACGAACTGAAGCGCCGCAATCTTGAACCTCTCTTTACGGAAAAGACCGGGCTGCTGCTCGACCCCT
ATTTTTCCGGGACGAAACTTGCATGGTTGCTGAACCATGTTCCGGGCCTGCGCGAGCGTGCGCAAAAAGGTCAAGTCTGC
TTCGGCACCATCGATAGCTGGCTGATCTACAAGCTCACCGGCGGCAAGGCGCATGTAACCGACGCCACGAATGCCTCGCG
TACATTGATCTATCATATCGGCGAAAACCGTTGGGATGATGAACTGCTGGACATATTGGGCATTCCAGCCGCCATGCTGC
CGGAAGTGAAGGACTGCGCGGCGGATTTCGGCATGACGGACCCTGCCCTTTTCGGCGTTTCCATTCCTATTCTCGGCGTA
GCGGGCGACCAGCAGGCGGCAGTGATCGGCAATGCCTGCTTCGAGCCAGGCATGATGAAATCCACCTACGGCACCGGATG
TTTTGCACTTCTCAACACCGGCACGGATCGCGTCACCTCCTCCAACCGGCTGCTCACCACCATCGCCTACCGGCTCGACG
GTGTTACGACCTATGCGCTGGAAGGTTCGATATTCATCGCGGGCGCGGCCGTACAATGGCTTCGTGACGAAATGGGCTTC
ATTTCGGTCGCATCGGAAGTAAGCGCGCTGGCCGAAAAGGCCGACCCGAACCAGCGCATCTATCTGGTTCCAGCCTTCAC
CGGGCTTGGCGCACCCTATTGGGACGCCGAAGCGCGCGGCGCAATCTTCGGGTTGACGCGCGGGACGGGCCGCGCAGAAT
TTGCCCGCGCAGCACTTGAAAGCGTGGCCTATCAGACCTTCGACCTTCTGGAAGCCATGCAAGGGGACTGGAAAGGCGCA
ACCAATCACACGGTGCTGCGCGTCGATGGAGGCATGGTTGCTTCCGACTGGACCATGCAGCGACTGGCCGACATTCTGAA
CGCACCCGTCGATCGCCCGGTTTTTCTCGAAACCACCGTGCTGGGCGCCGCATGGCTGGCCGCCTCCCGCGCAGGCATCT
GGCCGGATCGCAAGGGTTTTTCGGAACGCTGGCAGCGTGATTGCAGGTTTGAGCCGGCTATGCCGGAAAAAGAACGTGAA
AGCGCGATTGCGGGCTGGCGGGATAGCGTTTCACGCTGCCTCACACGACCGCAATAG

Upstream 100 bases:

>100_bases
AGGCTTGCTTCCCTTTTGCCGCTCTGTAAATCTGCATGTCAGGCTATGGTGACGGCTCCTGCCGTCCCCATTATCTGTTG
CCAAAAGGAATGCGGGAAGA

Downstream 100 bases:

>100_bases
AAAAGGCCAAGCGGCAAGCTTTCACTAGAAATAGTCTGAAGAATCCTGATCCCTCTGGAAATCACGAACGCGAGGCGATA
AAGCCAAGGGGGAACTACAT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSGYILAIDQGTTSTRSMLFDRNMRVVGLGQQEFTQHFPSSGWVEHDAEEIWKSVQSTIRIALAQAGISAADVAAIGITN
QRKTTVVWDRISGKPVHRAIVWQDRRTAQFCDELKRRNLEPLFTEKTGLLLDPYFSGTKLAWLLNHVPGLRERAQKGQVC
FGTIDSWLIYKLTGGKAHVTDATNASRTLIYHIGENRWDDELLDILGIPAAMLPEVKDCAADFGMTDPALFGVSIPILGV
AGDQQAAVIGNACFEPGMMKSTYGTGCFALLNTGTDRVTSSNRLLTTIAYRLDGVTTYALEGSIFIAGAAVQWLRDEMGF
ISVASEVSALAEKADPNQRIYLVPAFTGLGAPYWDAEARGAIFGLTRGTGRAEFARAALESVAYQTFDLLEAMQGDWKGA
TNHTVLRVDGGMVASDWTMQRLADILNAPVDRPVFLETTVLGAAWLAASRAGIWPDRKGFSERWQRDCRFEPAMPEKERE
SAIAGWRDSVSRCLTRPQ

Sequences:

>Translated_498_residues
MSGYILAIDQGTTSTRSMLFDRNMRVVGLGQQEFTQHFPSSGWVEHDAEEIWKSVQSTIRIALAQAGISAADVAAIGITN
QRKTTVVWDRISGKPVHRAIVWQDRRTAQFCDELKRRNLEPLFTEKTGLLLDPYFSGTKLAWLLNHVPGLRERAQKGQVC
FGTIDSWLIYKLTGGKAHVTDATNASRTLIYHIGENRWDDELLDILGIPAAMLPEVKDCAADFGMTDPALFGVSIPILGV
AGDQQAAVIGNACFEPGMMKSTYGTGCFALLNTGTDRVTSSNRLLTTIAYRLDGVTTYALEGSIFIAGAAVQWLRDEMGF
ISVASEVSALAEKADPNQRIYLVPAFTGLGAPYWDAEARGAIFGLTRGTGRAEFARAALESVAYQTFDLLEAMQGDWKGA
TNHTVLRVDGGMVASDWTMQRLADILNAPVDRPVFLETTVLGAAWLAASRAGIWPDRKGFSERWQRDCRFEPAMPEKERE
SAIAGWRDSVSRCLTRPQ
>Mature_497_residues
SGYILAIDQGTTSTRSMLFDRNMRVVGLGQQEFTQHFPSSGWVEHDAEEIWKSVQSTIRIALAQAGISAADVAAIGITNQ
RKTTVVWDRISGKPVHRAIVWQDRRTAQFCDELKRRNLEPLFTEKTGLLLDPYFSGTKLAWLLNHVPGLRERAQKGQVCF
GTIDSWLIYKLTGGKAHVTDATNASRTLIYHIGENRWDDELLDILGIPAAMLPEVKDCAADFGMTDPALFGVSIPILGVA
GDQQAAVIGNACFEPGMMKSTYGTGCFALLNTGTDRVTSSNRLLTTIAYRLDGVTTYALEGSIFIAGAAVQWLRDEMGFI
SVASEVSALAEKADPNQRIYLVPAFTGLGAPYWDAEARGAIFGLTRGTGRAEFARAALESVAYQTFDLLEAMQGDWKGAT
NHTVLRVDGGMVASDWTMQRLADILNAPVDRPVFLETTVLGAAWLAASRAGIWPDRKGFSERWQRDCRFEPAMPEKERES
AIAGWRDSVSRCLTRPQ

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=503, Percent_Identity=43.5387673956262, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI4504007, Length=503, Percent_Identity=43.5387673956262, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI42794763, Length=509, Percent_Identity=42.8290766208251, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI41393575, Length=503, Percent_Identity=42.1471172962227, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=30.2554027504912, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1790361, Length=492, Percent_Identity=52.0325203252033, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1790005, Length=480, Percent_Identity=26.25, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789987, Length=493, Percent_Identity=23.7322515212982, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787789, Length=501, Percent_Identity=25.748502994012, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=499, Percent_Identity=40.8817635270541, Blast_Score=355, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=23.9382239382239, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI71986147, Length=298, Percent_Identity=26.8456375838926, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321755, Length=487, Percent_Identity=37.1663244353183, Blast_Score=267, Evalue=2e-72,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=40.8203125, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24655555, Length=507, Percent_Identity=36.4891518737673, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24655560, Length=507, Percent_Identity=36.4891518737673, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24655570, Length=507, Percent_Identity=36.4891518737673, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24655574, Length=507, Percent_Identity=36.4891518737673, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24655565, Length=507, Percent_Identity=36.4891518737673, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24654573, Length=449, Percent_Identity=41.8708240534521, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=31.2380952380952, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=31.9105691056911, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24656367, Length=517, Percent_Identity=29.5938104448743, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI21357867, Length=528, Percent_Identity=28.7878787878788, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24656372, Length=351, Percent_Identity=31.3390313390313, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI45552909, Length=280, Percent_Identity=28.9285714285714, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54737; Mature: 54606

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYILAIDQGTTSTRSMLFDRNMRVVGLGQQEFTQHFPSSGWVEHDAEEIWKSVQSTIR
CCCEEEEEECCCCHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
IALAQAGISAADVAAIGITNQRKTTVVWDRISGKPVHRAIVWQDRRTAQFCDELKRRNLE
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCC
PLFTEKTGLLLDPYFSGTKLAWLLNHVPGLRERAQKGQVCFGTIDSWLIYKLTGGKAHVT
CEEECCCCCEECCCCCCCHHHHHHHHCCCHHHHHHCCCEEEEECCCEEEEEEECCEEEEE
DATNASRTLIYHIGENRWDDELLDILGIPAAMLPEVKDCAADFGMTDPALFGVSIPILGV
CCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCHHHEEEEEEEEE
AGDQQAAVIGNACFEPGMMKSTYGTGCFALLNTGTDRVTSSNRLLTTIAYRLDGVTTYAL
CCCCCCEEECHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEHHHHHHCCEEEEEE
EGSIFIAGAAVQWLRDEMGFISVASEVSALAEKADPNQRIYLVPAFTGLGAPYWDAEARG
CCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCC
AIFGLTRGTGRAEFARAALESVAYQTFDLLEAMQGDWKGATNHTVLRVDGGMVASDWTMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCHHHH
RLADILNAPVDRPVFLETTVLGAAWLAASRAGIWPDRKGFSERWQRDCRFEPAMPEKERE
HHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHH
SAIAGWRDSVSRCLTRPQ
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGYILAIDQGTTSTRSMLFDRNMRVVGLGQQEFTQHFPSSGWVEHDAEEIWKSVQSTIR
CCEEEEEECCCCHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
IALAQAGISAADVAAIGITNQRKTTVVWDRISGKPVHRAIVWQDRRTAQFCDELKRRNLE
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCC
PLFTEKTGLLLDPYFSGTKLAWLLNHVPGLRERAQKGQVCFGTIDSWLIYKLTGGKAHVT
CEEECCCCCEECCCCCCCHHHHHHHHCCCHHHHHHCCCEEEEECCCEEEEEEECCEEEEE
DATNASRTLIYHIGENRWDDELLDILGIPAAMLPEVKDCAADFGMTDPALFGVSIPILGV
CCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHCCCHHHHHHHCCCCCCHHHEEEEEEEEE
AGDQQAAVIGNACFEPGMMKSTYGTGCFALLNTGTDRVTSSNRLLTTIAYRLDGVTTYAL
CCCCCCEEECHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEHHHHHHCCEEEEEE
EGSIFIAGAAVQWLRDEMGFISVASEVSALAEKADPNQRIYLVPAFTGLGAPYWDAEARG
CCCEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCC
AIFGLTRGTGRAEFARAALESVAYQTFDLLEAMQGDWKGATNHTVLRVDGGMVASDWTMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCHHHH
RLADILNAPVDRPVFLETTVLGAAWLAASRAGIWPDRKGFSERWQRDCRFEPAMPEKERE
HHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHH
SAIAGWRDSVSRCLTRPQ
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA