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The map label for this gene is ackA [H]

Identifier: 163844562

GI number: 163844562

Start: 369084

End: 370289

Strand: Reverse

Name: ackA [H]

Synonym: BSUIS_B0391

Alternate gene names: 163844562

Gene position: 370289-369084 (Counterclockwise)

Preceding gene: 163844564

Following gene: 163844561

Centisome position: 26.43

GC content: 57.05

Gene sequence:

>1206_bases
GTGAAAAAGCAGCTTGTTACCTTCAATGCGGGTTCTTCCACGGTAAAGATCGGCGTATTCGAGTGCGATGAAAACGCGGT
CCGCAAGGTTTCAAAAGGCGATATCGACCTGACGGGATCACCCTTGCGATTCCGGCTGCGTGGCGAAAATGGCAATCTGG
ACATAGTCTTGTCAGCCAGCGGCGCGGACGAGATCACCACCATATTGGGTGAGCTTTTCGACAAGCTTGCCGAACATATC
GACCCGTCACGCATATTGGCTGTTGGCCACCGTGTCGTTTTCGGCGGCGACGATTTTGCGCAAGCCGTGCTCATCGACGA
CGACAATCTTGAGGCCATCGAACGCCTGTCGATTTTCGCGCCCCTGCACCAGCCCAAGAGCCTCGCCCTCATAAAAGCCG
TCAGAAAGATATTCCCGCATCTTGCACAGACGGCATCCTTCGACACGGCCTTTCACCAGACCATCCCCGATACCGTGCGC
CGCTTTGCCATTCCGCAGGAACTGCATGATCGCGGCATCAAGCGGTTCGGCTTTCACGGGCTTTCCTATAAATCCGTCAT
CGGCAATTTTTCGCAGCGCGCGCCGGAGCTTGCGGAAGGGAAGATCATTGCCGCACATCTGGGAAGCGGCGCCAGCCTTT
GCGCCATGCACAAGCGTAAAAGTCTCGATACCAGCATGAGCTTTTCCACGCTTGATGGAATTCCCATGGCAACACGATGC
GGGGCGCTGGACCCCGGCGTGCTTCTCCATTTGCTGCAACAGGAGAAAATGGAGGCCGATGCGCTTGCCGACCTTCTCTA
TCACCGTTCCGGCCTGCTTGGCCTTTCGGGCCTGAGCGGCGACACGCGGGATCTGCTCGAAAGTGATGCGCCGCAGGCGC
ACACGGCGCTCGATATATTCGCGCTGCGCATTGCCGGGGAAATCTGTCGCCTTGCCTCAACGCTCAATGGCCTTGATGCC
GTGATTTTTACCGCAGGCATAGGCGAACACCAGCCTGCGATAAGAGCCGCCATATGCCAGCGCCTCGCCTGGCAAGGCGT
CGAACTGGACCCTGCGGCAAATGATGAAAATGCTTTCTGCATCACAAGCCGCACAAGCCGCACGGCCGCATTCGTTCTGC
CAACCGACGAAGAACAGATCATCGCCTCGGAAACATTTGAAATCATTCGGCAAATGCCAGCAACCGCCAGAAGCGCGTTC
GCATAA

Upstream 100 bases:

>100_bases
TGCGTGTTAAGAGCATAATCCTGTCCGAGTAAAAACGGGGACCGAAAAATTATGCTTCAAATAGAAGAGTTTAGAAACGC
GGTCTAAAGGAGCTGCCCCG

Downstream 100 bases:

>100_bases
ATTCAGCCAAGGAGACTTATATGAGCACTGTGCCAGCAAAAGGGCCTCTCACACCGCAGCAGCTTTCACTCATCAACCGT
TACTGGCGCGCGGCGAATTA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKQLVTFNAGSSTVKIGVFECDENAVRKVSKGDIDLTGSPLRFRLRGENGNLDIVLSASGADEITTILGELFDKLAEHI
DPSRILAVGHRVVFGGDDFAQAVLIDDDNLEAIERLSIFAPLHQPKSLALIKAVRKIFPHLAQTASFDTAFHQTIPDTVR
RFAIPQELHDRGIKRFGFHGLSYKSVIGNFSQRAPELAEGKIIAAHLGSGASLCAMHKRKSLDTSMSFSTLDGIPMATRC
GALDPGVLLHLLQQEKMEADALADLLYHRSGLLGLSGLSGDTRDLLESDAPQAHTALDIFALRIAGEICRLASTLNGLDA
VIFTAGIGEHQPAIRAAICQRLAWQGVELDPAANDENAFCITSRTSRTAAFVLPTDEEQIIASETFEIIRQMPATARSAF
A

Sequences:

>Translated_401_residues
MKKQLVTFNAGSSTVKIGVFECDENAVRKVSKGDIDLTGSPLRFRLRGENGNLDIVLSASGADEITTILGELFDKLAEHI
DPSRILAVGHRVVFGGDDFAQAVLIDDDNLEAIERLSIFAPLHQPKSLALIKAVRKIFPHLAQTASFDTAFHQTIPDTVR
RFAIPQELHDRGIKRFGFHGLSYKSVIGNFSQRAPELAEGKIIAAHLGSGASLCAMHKRKSLDTSMSFSTLDGIPMATRC
GALDPGVLLHLLQQEKMEADALADLLYHRSGLLGLSGLSGDTRDLLESDAPQAHTALDIFALRIAGEICRLASTLNGLDA
VIFTAGIGEHQPAIRAAICQRLAWQGVELDPAANDENAFCITSRTSRTAAFVLPTDEEQIIASETFEIIRQMPATARSAF
A
>Mature_401_residues
MKKQLVTFNAGSSTVKIGVFECDENAVRKVSKGDIDLTGSPLRFRLRGENGNLDIVLSASGADEITTILGELFDKLAEHI
DPSRILAVGHRVVFGGDDFAQAVLIDDDNLEAIERLSIFAPLHQPKSLALIKAVRKIFPHLAQTASFDTAFHQTIPDTVR
RFAIPQELHDRGIKRFGFHGLSYKSVIGNFSQRAPELAEGKIIAAHLGSGASLCAMHKRKSLDTSMSFSTLDGIPMATRC
GALDPGVLLHLLQQEKMEADALADLLYHRSGLLGLSGLSGDTRDLLESDAPQAHTALDIFALRIAGEICRLASTLNGLDA
VIFTAGIGEHQPAIRAAICQRLAWQGVELDPAANDENAFCITSRTSRTAAFVLPTDEEQIIASETFEIIRQMPATARSAF
A

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=309, Percent_Identity=37.2168284789644, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1788633, Length=398, Percent_Identity=32.1608040201005, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43362; Mature: 43362

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQLVTFNAGSSTVKIGVFECDENAVRKVSKGDIDLTGSPLRFRLRGENGNLDIVLSAS
CCCEEEEEECCCCEEEEEEEECCCHHHHHHCCCCCEECCCCEEEEEECCCCCEEEEEECC
GADEITTILGELFDKLAEHIDPSRILAVGHRVVFGGDDFAQAVLIDDDNLEAIERLSIFA
CCHHHHHHHHHHHHHHHHHCCHHHEEEECCEEEECCCCCEEEEEECCCCHHHHHHHHHHH
PLHQPKSLALIKAVRKIFPHLAQTASFDTAFHQTIPDTVRRFAIPQELHDRGIKRFGFHG
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCCC
LSYKSVIGNFSQRAPELAEGKIIAAHLGSGASLCAMHKRKSLDTSMSFSTLDGIPMATRC
CCHHHHHCCHHHCCCCHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHCCCCCCHHHC
GALDPGVLLHLLQQEKMEADALADLLYHRSGLLGLSGLSGDTRDLLESDAPQAHTALDIF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHCCCCCHHHHHHHH
ALRIAGEICRLASTLNGLDAVIFTAGIGEHQPAIRAAICQRLAWQGVELDPAANDENAFC
HHHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
ITSRTSRTAAFVLPTDEEQIIASETFEIIRQMPATARSAFA
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MKKQLVTFNAGSSTVKIGVFECDENAVRKVSKGDIDLTGSPLRFRLRGENGNLDIVLSAS
CCCEEEEEECCCCEEEEEEEECCCHHHHHHCCCCCEECCCCEEEEEECCCCCEEEEEECC
GADEITTILGELFDKLAEHIDPSRILAVGHRVVFGGDDFAQAVLIDDDNLEAIERLSIFA
CCHHHHHHHHHHHHHHHHHCCHHHEEEECCEEEECCCCCEEEEEECCCCHHHHHHHHHHH
PLHQPKSLALIKAVRKIFPHLAQTASFDTAFHQTIPDTVRRFAIPQELHDRGIKRFGFHG
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCCC
LSYKSVIGNFSQRAPELAEGKIIAAHLGSGASLCAMHKRKSLDTSMSFSTLDGIPMATRC
CCHHHHHCCHHHCCCCHHCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHCCCCCCHHHC
GALDPGVLLHLLQQEKMEADALADLLYHRSGLLGLSGLSGDTRDLLESDAPQAHTALDIF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHCCCCCHHHHHHHH
ALRIAGEICRLASTLNGLDAVIFTAGIGEHQPAIRAAICQRLAWQGVELDPAANDENAFC
HHHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
ITSRTSRTAAFVLPTDEEQIIASETFEIIRQMPATARSAFA
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]