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The map label for this gene is narK [H]

Identifier: 163844482

GI number: 163844482

Start: 284902

End: 287589

Strand: Reverse

Name: narK [H]

Synonym: BSUIS_B0306

Alternate gene names: 163844482

Gene position: 287589-284902 (Counterclockwise)

Preceding gene: 163844484

Following gene: 163844481

Centisome position: 20.53

GC content: 59.9

Gene sequence:

>2688_bases
ATGAGCACAACCGCTTTTACAGTCTGTTTTGCGGTCTGGACCATCTTTGCTATCATCGGCATTGAAATTCGATCCGAGCT
TGGCCTGACTGAGACACAGTTCGGCCTGCTTGTTGGCACGCCCATCCTGACCGGATCGCTGATCCGCGTCATTCTGGGCA
TATGGGCGGACCGCTATGGCGGCCGTGCCATCTTCACGCTCACAATGCTGGTTTCGGCTGTCGCCACCTATCTCCTGACC
TATGCCACGACCTATCCGCAAATGCTGCTCGCAGCCCTCGGCGTCGGCGTGGCGGGCGGGTCGTTCTCGGTCGGCGTCGC
TTATGTCTCGCGCTGGTATCCACCTGAAAAGCAGGGCGTTGCGCTCGGCATTTTCGGCGCGGGCAATGTCGGCGCGGCGG
TGACGAAGTTCTTTGCACCGATGGTCATGGTTGCATTTGGCTGGCATACGGTTGCCGAAGTCTGGGCGGCCGCAATGGTT
GTCATGGCGCTGGTCTACTATTTCACCACCAACGACGATCCCGTTCTGGTCGAACGCCGTCGCAAGGGTGAAAAGCCCAC
CAGCACGCTGATGGAGCTGGAACCACTCAAGAACATTCAGGTCTGGCGTTTCTCGCTTTATTACTTCTTCGTGTTCGGCG
CCTTTGTGGCGCTCTCCCTGTGGCTGCCGCAATATCTGATCCATGTCTATGGCGTCGATATCCGTTTTGCCGGCATCATC
GCGGCATTCTTCTCCATCCCGGCCAGCCTTTTCCGCGCTTATGGCGGGCATCTTTCAGATGTCTACGGCGCGCGCAGGGT
CATGTACTGGACCTTCGCCGTCTCGCTGGTGGCGACCTTCATTCTGTCTTATCCGCCAACGGATTATGTCATCCAGAGCG
ATAATGGCCCGCTGGCCTTCCACGCCGAAATGGGCGTAATCGGTTTCACGGTAACGGTCTTCATCCTCGGCTTCTTCATG
GCCCTGGGCAAGGCAGCCGTCTTCAAGCATATCCCGGTCTATTATCCCGGCAATGTCGGCGCTGTCGGCGGTCTCGTCGG
CATGATCGGCGGCCTTGGCGGCTTCATCCTGCCGATCCTGTTCGGCGTGCTGCTGGACCAGACCGGCCTGTGGACGAGCT
GCTTCATGCTGCTCTTCGTCATCGTCGTCGTGTCCTTCGTGTGGATGCATGTCTCCATTCGCCAGATGGAACACGCAGCC
GAAGGCAAGGCTACGGAAGAACTTCCCGCCTTCGCCGAATTGCAGGGCCTGAAAAAGGCTGAGGTCAAGCCTGCGACGGG
CGAATCCGTCCTCACCGACTGGCGGCCTGACGATCCGGCCTTCTGGGAACAGAAAGGCAGGCGCATCGCCAAGCGCAATC
TGTGGCTCTCCATCCCGGCGCTTCTCCTCTCCTTCGCCATCTGGCAGGTCTGGTCCGTCGTCGTGGCAAAACTGCCGCTG
GTGGGCTATCAGTTCACGACCGACCAGCTCTTCTGGCTGGCCGCTCTGCCCGGCATTTCGGGCGCAACCCTGCGTATCTT
CTATTCCTTCATGGTCCCGATCTTTGGCGGCCGCCTTTGGACGACGCTTACCACCTGGTCGCTCGTCATTCCGGCCATCG
GCATCGGCTATGCGGTCCAGAATCCGGATACGCCTTACTTCATCTTCCTCCTTCTGGCGCTCTTCTGCGGCTTTGGCGGC
GGTAACTTCGCCTCCTCCATGTCCAACATTTCCTTCTTCTTCCCGAAGACGGAAAAGGGCAATGCGGCGGCACTGAACGC
AGGCCTCGGCAATCTCGGCGTCAGCGTCGTGCAGTTCGTCGTTCCGCTGGTCATCACCGCAGGCATCTTCGGCAAGCTCG
GCGGCAACCCGGCAATGGTCGCAACCGCGGCTGGCGAAGCCCCGCTCTGGCTCCAGAACGCCGGCTTCATCTTCGTGCCG
TTCATCATCGTCACGGCTTTCGCCAACTGGTTCGGCATGAACGACATCGCCTCTGCCAAGGCTTCGTTTGCCGATCAGGC
CGTCATCTTCCGCCGCAGGCATAACTGGATCATGTGCTGGCTTTACACCGGTACGTTCGGTTCGTTCATCGGCTATTCGG
CAGGTTTCCCGCTGCTCACCAACATCCTGTTCCCCGAGGTCAATGCGCTGCAATTCGCCTTCCTCGGACCTTTGGTCGGT
GCATTGTCGCGGTCGGGTTCCGGCTGGCTGGCAGACAAATACGGCGGTGGTCGCGTTACCATCTGGGCTTTCATCCTGAT
GATGGTTGGCGTGGCAGGCGTGCTCTACTTCGTCGGCATCAAGGATGAGCCGAATGCCTTCTGGGGCTTCTTCGCCGCCT
TCATCCTGCTGTTCTTCGCCACCGGCATTGGCAATGCCTCCACCTTCCAGATGATCCCGGCGATCATGGCGAAGGATATG
GAACGGCTAATGCCCATGGCTTCGGCAGAAGAACGCCGCCGCCAGGCAGACAAGGAATCCGCAGCCATCACCGGCTTCAC
CTCGGCCATCGCTGCCTTCGGCGCCTTCTTCATCCCCAAGGGATACGGCACCTCGATCAGCCTGACCGGAGGACCGGAAA
TGGCGCTGTGGGCATTCCTGATCTTCTACGTGACCTGCCTCATCATCACCTGGGGCGTCTATACCCGAAAAGGCGGACTG
CTCTACGACGTCGAGCGCCGCCTCAAGCCGGCCGCAGCCGCCGCTTGA

Upstream 100 bases:

>100_bases
GCATAAGTGCAAACGAAACCTCCCATGGTATTCAATTCGGAGTACGCCTATGCAACATGTCGCAGGGGAAAACCCCAGGG
CGCAGACTACGGCGCTAGCG

Downstream 100 bases:

>100_bases
AGAAAAACTGAGAGGATATAATCATGAGTCTACTCCTCGACCGCCTGAACTTCCTGGCACGCAGGAATGTCGGCACCTTC
TCGGAAGGGCACGGCGTCAC

Product: nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 895; Mature: 894

Protein sequence:

>895_residues
MSTTAFTVCFAVWTIFAIIGIEIRSELGLTETQFGLLVGTPILTGSLIRVILGIWADRYGGRAIFTLTMLVSAVATYLLT
YATTYPQMLLAALGVGVAGGSFSVGVAYVSRWYPPEKQGVALGIFGAGNVGAAVTKFFAPMVMVAFGWHTVAEVWAAAMV
VMALVYYFTTNDDPVLVERRRKGEKPTSTLMELEPLKNIQVWRFSLYYFFVFGAFVALSLWLPQYLIHVYGVDIRFAGII
AAFFSIPASLFRAYGGHLSDVYGARRVMYWTFAVSLVATFILSYPPTDYVIQSDNGPLAFHAEMGVIGFTVTVFILGFFM
ALGKAAVFKHIPVYYPGNVGAVGGLVGMIGGLGGFILPILFGVLLDQTGLWTSCFMLLFVIVVVSFVWMHVSIRQMEHAA
EGKATEELPAFAELQGLKKAEVKPATGESVLTDWRPDDPAFWEQKGRRIAKRNLWLSIPALLLSFAIWQVWSVVVAKLPL
VGYQFTTDQLFWLAALPGISGATLRIFYSFMVPIFGGRLWTTLTTWSLVIPAIGIGYAVQNPDTPYFIFLLLALFCGFGG
GNFASSMSNISFFFPKTEKGNAAALNAGLGNLGVSVVQFVVPLVITAGIFGKLGGNPAMVATAAGEAPLWLQNAGFIFVP
FIIVTAFANWFGMNDIASAKASFADQAVIFRRRHNWIMCWLYTGTFGSFIGYSAGFPLLTNILFPEVNALQFAFLGPLVG
ALSRSGSGWLADKYGGGRVTIWAFILMMVGVAGVLYFVGIKDEPNAFWGFFAAFILLFFATGIGNASTFQMIPAIMAKDM
ERLMPMASAEERRRQADKESAAITGFTSAIAAFGAFFIPKGYGTSISLTGGPEMALWAFLIFYVTCLIITWGVYTRKGGL
LYDVERRLKPAAAAA

Sequences:

>Translated_895_residues
MSTTAFTVCFAVWTIFAIIGIEIRSELGLTETQFGLLVGTPILTGSLIRVILGIWADRYGGRAIFTLTMLVSAVATYLLT
YATTYPQMLLAALGVGVAGGSFSVGVAYVSRWYPPEKQGVALGIFGAGNVGAAVTKFFAPMVMVAFGWHTVAEVWAAAMV
VMALVYYFTTNDDPVLVERRRKGEKPTSTLMELEPLKNIQVWRFSLYYFFVFGAFVALSLWLPQYLIHVYGVDIRFAGII
AAFFSIPASLFRAYGGHLSDVYGARRVMYWTFAVSLVATFILSYPPTDYVIQSDNGPLAFHAEMGVIGFTVTVFILGFFM
ALGKAAVFKHIPVYYPGNVGAVGGLVGMIGGLGGFILPILFGVLLDQTGLWTSCFMLLFVIVVVSFVWMHVSIRQMEHAA
EGKATEELPAFAELQGLKKAEVKPATGESVLTDWRPDDPAFWEQKGRRIAKRNLWLSIPALLLSFAIWQVWSVVVAKLPL
VGYQFTTDQLFWLAALPGISGATLRIFYSFMVPIFGGRLWTTLTTWSLVIPAIGIGYAVQNPDTPYFIFLLLALFCGFGG
GNFASSMSNISFFFPKTEKGNAAALNAGLGNLGVSVVQFVVPLVITAGIFGKLGGNPAMVATAAGEAPLWLQNAGFIFVP
FIIVTAFANWFGMNDIASAKASFADQAVIFRRRHNWIMCWLYTGTFGSFIGYSAGFPLLTNILFPEVNALQFAFLGPLVG
ALSRSGSGWLADKYGGGRVTIWAFILMMVGVAGVLYFVGIKDEPNAFWGFFAAFILLFFATGIGNASTFQMIPAIMAKDM
ERLMPMASAEERRRQADKESAAITGFTSAIAAFGAFFIPKGYGTSISLTGGPEMALWAFLIFYVTCLIITWGVYTRKGGL
LYDVERRLKPAAAAA
>Mature_894_residues
STTAFTVCFAVWTIFAIIGIEIRSELGLTETQFGLLVGTPILTGSLIRVILGIWADRYGGRAIFTLTMLVSAVATYLLTY
ATTYPQMLLAALGVGVAGGSFSVGVAYVSRWYPPEKQGVALGIFGAGNVGAAVTKFFAPMVMVAFGWHTVAEVWAAAMVV
MALVYYFTTNDDPVLVERRRKGEKPTSTLMELEPLKNIQVWRFSLYYFFVFGAFVALSLWLPQYLIHVYGVDIRFAGIIA
AFFSIPASLFRAYGGHLSDVYGARRVMYWTFAVSLVATFILSYPPTDYVIQSDNGPLAFHAEMGVIGFTVTVFILGFFMA
LGKAAVFKHIPVYYPGNVGAVGGLVGMIGGLGGFILPILFGVLLDQTGLWTSCFMLLFVIVVVSFVWMHVSIRQMEHAAE
GKATEELPAFAELQGLKKAEVKPATGESVLTDWRPDDPAFWEQKGRRIAKRNLWLSIPALLLSFAIWQVWSVVVAKLPLV
GYQFTTDQLFWLAALPGISGATLRIFYSFMVPIFGGRLWTTLTTWSLVIPAIGIGYAVQNPDTPYFIFLLLALFCGFGGG
NFASSMSNISFFFPKTEKGNAAALNAGLGNLGVSVVQFVVPLVITAGIFGKLGGNPAMVATAAGEAPLWLQNAGFIFVPF
IIVTAFANWFGMNDIASAKASFADQAVIFRRRHNWIMCWLYTGTFGSFIGYSAGFPLLTNILFPEVNALQFAFLGPLVGA
LSRSGSGWLADKYGGGRVTIWAFILMMVGVAGVLYFVGIKDEPNAFWGFFAAFILLFFATGIGNASTFQMIPAIMAKDME
RLMPMASAEERRRQADKESAAITGFTSAIAAFGAFFIPKGYGTSISLTGGPEMALWAFLIFYVTCLIITWGVYTRKGGLL
YDVERRLKPAAAAA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=458, Percent_Identity=52.8384279475983, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI1787743, Length=450, Percent_Identity=50.6666666666667, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17561094, Length=209, Percent_Identity=24.8803827751196, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 97414; Mature: 97282

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTAFTVCFAVWTIFAIIGIEIRSELGLTETQFGLLVGTPILTGSLIRVILGIWADRYG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GRAIFTLTMLVSAVATYLLTYATTYPQMLLAALGVGVAGGSFSVGVAYVSRWYPPEKQGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCE
ALGIFGAGNVGAAVTKFFAPMVMVAFGWHTVAEVWAAAMVVMALVYYFTTNDDPVLVERR
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHH
RKGEKPTSTLMELEPLKNIQVWRFSLYYFFVFGAFVALSLWLPQYLIHVYGVDIRFAGII
HCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AAFFSIPASLFRAYGGHLSDVYGARRVMYWTFAVSLVATFILSYPPTDYVIQSDNGPLAF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEE
HAEMGVIGFTVTVFILGFFMALGKAAVFKHIPVYYPGNVGAVGGLVGMIGGLGGFILPIL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
FGVLLDQTGLWTSCFMLLFVIVVVSFVWMHVSIRQMEHAAEGKATEELPAFAELQGLKKA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHCCHHC
EVKPATGESVLTDWRPDDPAFWEQKGRRIAKRNLWLSIPALLLSFAIWQVWSVVVAKLPL
CCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VGYQFTTDQLFWLAALPGISGATLRIFYSFMVPIFGGRLWTTLTTWSLVIPAIGIGYAVQ
CCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEC
NPDTPYFIFLLLALFCGFGGGNFASSMSNISFFFPKTEKGNAAALNAGLGNLGVSVVQFV
CCCCHHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHH
VPLVITAGIFGKLGGNPAMVATAAGEAPLWLQNAGFIFVPFIIVTAFANWFGMNDIASAK
HHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHH
ASFADQAVIFRRRHNWIMCWLYTGTFGSFIGYSAGFPLLTNILFPEVNALQFAFLGPLVG
HHHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
ALSRSGSGWLADKYGGGRVTIWAFILMMVGVAGVLYFVGIKDEPNAFWGFFAAFILLFFA
HHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHH
TGIGNASTFQMIPAIMAKDMERLMPMASAEERRRQADKESAAITGFTSAIAAFGAFFIPK
HCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYGTSISLTGGPEMALWAFLIFYVTCLIITWGVYTRKGGLLYDVERRLKPAAAAA
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCHHCCC
>Mature Secondary Structure 
STTAFTVCFAVWTIFAIIGIEIRSELGLTETQFGLLVGTPILTGSLIRVILGIWADRYG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GRAIFTLTMLVSAVATYLLTYATTYPQMLLAALGVGVAGGSFSVGVAYVSRWYPPEKQGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCE
ALGIFGAGNVGAAVTKFFAPMVMVAFGWHTVAEVWAAAMVVMALVYYFTTNDDPVLVERR
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHH
RKGEKPTSTLMELEPLKNIQVWRFSLYYFFVFGAFVALSLWLPQYLIHVYGVDIRFAGII
HCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AAFFSIPASLFRAYGGHLSDVYGARRVMYWTFAVSLVATFILSYPPTDYVIQSDNGPLAF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEE
HAEMGVIGFTVTVFILGFFMALGKAAVFKHIPVYYPGNVGAVGGLVGMIGGLGGFILPIL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
FGVLLDQTGLWTSCFMLLFVIVVVSFVWMHVSIRQMEHAAEGKATEELPAFAELQGLKKA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHCCHHC
EVKPATGESVLTDWRPDDPAFWEQKGRRIAKRNLWLSIPALLLSFAIWQVWSVVVAKLPL
CCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VGYQFTTDQLFWLAALPGISGATLRIFYSFMVPIFGGRLWTTLTTWSLVIPAIGIGYAVQ
CCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEC
NPDTPYFIFLLLALFCGFGGGNFASSMSNISFFFPKTEKGNAAALNAGLGNLGVSVVQFV
CCCCHHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHH
VPLVITAGIFGKLGGNPAMVATAAGEAPLWLQNAGFIFVPFIIVTAFANWFGMNDIASAK
HHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHH
ASFADQAVIFRRRHNWIMCWLYTGTFGSFIGYSAGFPLLTNILFPEVNALQFAFLGPLVG
HHHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
ALSRSGSGWLADKYGGGRVTIWAFILMMVGVAGVLYFVGIKDEPNAFWGFFAAFILLFFA
HHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHH
TGIGNASTFQMIPAIMAKDMERLMPMASAEERRRQADKESAAITGFTSAIAAFGAFFIPK
HCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYGTSISLTGGPEMALWAFLIFYVTCLIITWGVYTRKGGLLYDVERRLKPAAAAA
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]