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The map label for this gene is narH [H]

Identifier: 163844480

GI number: 163844480

Start: 279579

End: 281117

Strand: Reverse

Name: narH [H]

Synonym: BSUIS_B0304

Alternate gene names: 163844480

Gene position: 281117-279579 (Counterclockwise)

Preceding gene: 163844481

Following gene: 163844479

Centisome position: 20.07

GC content: 59.39

Gene sequence:

>1539_bases
ATGAAAATTCGCGCTCAAATCGGAATGGTGCTGAATCTCGACAAGTGTATCGGCTGCCACACCTGCTCCATCACGTGCAA
AAACGTCTGGACCAATCGTGAAGGCGTCGAATATGCCTGGTTCAACAATGTCGAGACCAAGCCCGGCGTCGGTTTTCCCA
AGGAATGGGAAAACCAGAAGAAGTGGAACGGCGGCTGGGTTCGCAAGAAGAACGGCAAGATCGAGCCGAAGATGGGCGCA
AAATGGCGCATCCTCGCCAAGATTTTCGCCAATCCCGACCTGCCGGAAATCGACGATTATTACGAACCGTTCGATTTCGA
TTACGGCCATCTCCAGACGGCAGGCGAAAGCCGCACCATGCCGACCGCCCGTCCGCGCTCGCTGATCTCCGGCGAGCGGA
TGGAGAAGATCGAATGGGGCCCGAACTGGGAAGAAATTCTCGGCGGCGAATTCGCCAAGCGCTCGAAGGACTACAATTTT
GAGGGCGTGGAGAAGGAAATCTACGGCCAGTTTGAAAACACCTTCATGATGTATCTGCCGCGCCTGTGCGAGCACTGCCT
CAACCCGACCTGCGTTGCGGCCTGCCCGTCCGGTGCGATCTACAAGCGCGAAGATGACGGCATCGTCCTGATCGATCAGG
AAAAATGCCGCGGCTGGCGCATGTGCGTTTCCGGCTGCCCCTACAAGAAGATTTACTACAACTGGAGCTCCGGCAAGTCC
GAGAAGTGCATCTTCTGCTATCCGCGCATCGAAAGCGGCCAGCCGACGGTCTGCTCGGAAACCTGTGTGGGCCGCATCCG
CTATCTGGGCGTGATGCTCTATGATGCCGACCGCATTTCGGAAGCCGCTTCCACGGCCAACGAACAGGATCTCTATCAGG
AACAGCTCAACATCTTCCTCGATCCGAACGATCCTGCGGTCATCGAGCAGGCCCGCAAGGACGGCGTGCCGGACGCATGG
CTGGAAGCAGCCAAGCATTCGCCGGTCTACAAGATGGCCATGGACTGGAAGATCGCCTTCCCGTTCCATCCTGAATACCG
CACCCTGCCGATGGTCTGGTATGTGCCGCCGCTCTCCCCGCTTCAGTCGGCCGCGCAGGCCGGCAAGCTGGGCATGGACG
GCCCGCTGCCGGATGTGCGCTCCATGCGCATCCCGGTCCGCTACCTCGCCAACCTGCTGACCGCAGGCAAGGAAAAGCCG
GTCATTTCCGCGCTGGAACGTATGCTGGCCATGCGCGCCTATATGCGCGCCAAGACCATCGATGGCGTCATCGACGAGGC
AATTGCCGACCGCGTCGGCATGAGCGGCGCGATGATCGAGGAAATGTACAACATCATGGCCATTGCCAATTACGAAGACC
GCTTCGTCATCCCGACGTCGCACCGCGAGATCACCGAGGACGCCTATGACGTTCGCGGCTCCTGCGGCTTCACCTTCGGC
AATGGCTGCTCCGGCGGCACGTCCACGGAAGACCTGTTCGGCGCCAAGCGCCAGAAAGTCGTGCAGACCCCGACGGATAT
TTTCAGGGAGCAGGTCTGA

Upstream 100 bases:

>100_bases
GCGACGAATTCGTGGTGGTGCGCAAGCTGGACAAGGTGGACTGGATGGAAGGTCCGCTCGAAGAAGGCAACAAGTCGTCC
CAGGTTAAGGAGGCAGCAGA

Downstream 100 bases:

>100_bases
TGAACCGAACCCTGAAGATCATTTCGCTGCTCCTCTCCTACCCCTCGCCTGAATTGCAGGCGGGGCGGGCAGAGCTGAAA
GCGGCGCTTGAGGGGGAGAA

Product: nitrate reductase, beta subunit

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKIRAQIGMVLNLDKCIGCHTCSITCKNVWTNREGVEYAWFNNVETKPGVGFPKEWENQKKWNGGWVRKKNGKIEPKMGA
KWRILAKIFANPDLPEIDDYYEPFDFDYGHLQTAGESRTMPTARPRSLISGERMEKIEWGPNWEEILGGEFAKRSKDYNF
EGVEKEIYGQFENTFMMYLPRLCEHCLNPTCVAACPSGAIYKREDDGIVLIDQEKCRGWRMCVSGCPYKKIYYNWSSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVMLYDADRISEAASTANEQDLYQEQLNIFLDPNDPAVIEQARKDGVPDAW
LEAAKHSPVYKMAMDWKIAFPFHPEYRTLPMVWYVPPLSPLQSAAQAGKLGMDGPLPDVRSMRIPVRYLANLLTAGKEKP
VISALERMLAMRAYMRAKTIDGVIDEAIADRVGMSGAMIEEMYNIMAIANYEDRFVIPTSHREITEDAYDVRGSCGFTFG
NGCSGGTSTEDLFGAKRQKVVQTPTDIFREQV

Sequences:

>Translated_512_residues
MKIRAQIGMVLNLDKCIGCHTCSITCKNVWTNREGVEYAWFNNVETKPGVGFPKEWENQKKWNGGWVRKKNGKIEPKMGA
KWRILAKIFANPDLPEIDDYYEPFDFDYGHLQTAGESRTMPTARPRSLISGERMEKIEWGPNWEEILGGEFAKRSKDYNF
EGVEKEIYGQFENTFMMYLPRLCEHCLNPTCVAACPSGAIYKREDDGIVLIDQEKCRGWRMCVSGCPYKKIYYNWSSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVMLYDADRISEAASTANEQDLYQEQLNIFLDPNDPAVIEQARKDGVPDAW
LEAAKHSPVYKMAMDWKIAFPFHPEYRTLPMVWYVPPLSPLQSAAQAGKLGMDGPLPDVRSMRIPVRYLANLLTAGKEKP
VISALERMLAMRAYMRAKTIDGVIDEAIADRVGMSGAMIEEMYNIMAIANYEDRFVIPTSHREITEDAYDVRGSCGFTFG
NGCSGGTSTEDLFGAKRQKVVQTPTDIFREQV
>Mature_512_residues
MKIRAQIGMVLNLDKCIGCHTCSITCKNVWTNREGVEYAWFNNVETKPGVGFPKEWENQKKWNGGWVRKKNGKIEPKMGA
KWRILAKIFANPDLPEIDDYYEPFDFDYGHLQTAGESRTMPTARPRSLISGERMEKIEWGPNWEEILGGEFAKRSKDYNF
EGVEKEIYGQFENTFMMYLPRLCEHCLNPTCVAACPSGAIYKREDDGIVLIDQEKCRGWRMCVSGCPYKKIYYNWSSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVMLYDADRISEAASTANEQDLYQEQLNIFLDPNDPAVIEQARKDGVPDAW
LEAAKHSPVYKMAMDWKIAFPFHPEYRTLPMVWYVPPLSPLQSAAQAGKLGMDGPLPDVRSMRIPVRYLANLLTAGKEKP
VISALERMLAMRAYMRAKTIDGVIDEAIADRVGMSGAMIEEMYNIMAIANYEDRFVIPTSHREITEDAYDVRGSCGFTFG
NGCSGGTSTEDLFGAKRQKVVQTPTDIFREQV

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=498, Percent_Identity=72.4899598393574, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=498, Percent_Identity=71.4859437751004, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=36.3636363636364, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=36.3636363636364, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=25.531914893617, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789370, Length=107, Percent_Identity=37.3831775700935, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=35.632183908046, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=34.4827586206897, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58178; Mature: 58178

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQIGMVLNLDKCIGCHTCSITCKNVWTNREGVEYAWFNNVETKPGVGFPKEWENQK
CEEHEEECEEEEHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCC
KWNGGWVRKKNGKIEPKMGAKWRILAKIFANPDLPEIDDYYEPFDFDYGHLQTAGESRTM
CCCCCEEECCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHCCCCCCCCCCHHCCCCCCCCC
PTARPRSLISGERMEKIEWGPNWEEILGGEFAKRSKDYNFEGVEKEIYGQFENTFMMYLP
CCCCCHHHHCCCHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RLCEHCLNPTCVAACPSGAIYKREDDGIVLIDQEKCRGWRMCVSGCPYKKIYYNWSSGKS
HHHHHHCCCEEEEECCCCCEEEECCCCEEEEEHHHCCCHHHHHCCCCCCEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVMLYDADRISEAASTANEQDLYQEQLNIFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECHHHHHHHHHCCCHHHHHHHHCEEEE
DPNDPAVIEQARKDGVPDAWLEAAKHSPVYKMAMDWKIAFPFHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCEEEEEECCCCHH
LQSAAQAGKLGMDGPLPDVRSMRIPVRYLANLLTAGKEKPVISALERMLAMRAYMRAKTI
HHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DGVIDEAIADRVGMSGAMIEEMYNIMAIANYEDRFVIPTSHREITEDAYDVRGSCGFTFG
HHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCEEECCCCCHHHHHHHHCCCCCCCCCC
NGCSGGTSTEDLFGAKRQKVVQTPTDIFREQV
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MKIRAQIGMVLNLDKCIGCHTCSITCKNVWTNREGVEYAWFNNVETKPGVGFPKEWENQK
CEEHEEECEEEEHHHHCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCC
KWNGGWVRKKNGKIEPKMGAKWRILAKIFANPDLPEIDDYYEPFDFDYGHLQTAGESRTM
CCCCCEEECCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHCCCCCCCCCCHHCCCCCCCCC
PTARPRSLISGERMEKIEWGPNWEEILGGEFAKRSKDYNFEGVEKEIYGQFENTFMMYLP
CCCCCHHHHCCCHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RLCEHCLNPTCVAACPSGAIYKREDDGIVLIDQEKCRGWRMCVSGCPYKKIYYNWSSGKS
HHHHHHCCCEEEEECCCCCEEEECCCCEEEEEHHHCCCHHHHHCCCCCCEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVMLYDADRISEAASTANEQDLYQEQLNIFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECHHHHHHHHHCCCHHHHHHHHCEEEE
DPNDPAVIEQARKDGVPDAWLEAAKHSPVYKMAMDWKIAFPFHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCEEEEEECCCCHH
LQSAAQAGKLGMDGPLPDVRSMRIPVRYLANLLTAGKEKPVISALERMLAMRAYMRAKTI
HHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DGVIDEAIADRVGMSGAMIEEMYNIMAIANYEDRFVIPTSHREITEDAYDVRGSCGFTFG
HHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCEEECCCCCHHHHHHHHCCCCCCCCCC
NGCSGGTSTEDLFGAKRQKVVQTPTDIFREQV
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]