Click here to switch to the map view.

The map label for this gene is kefBC [H]

Identifier: 163844397

GI number: 163844397

Start: 196157

End: 198064

Strand: Reverse

Name: kefBC [H]

Synonym: BSUIS_B0214

Alternate gene names: 163844397

Gene position: 198064-196157 (Counterclockwise)

Preceding gene: 163844404

Following gene: 163844392

Centisome position: 14.14

GC content: 59.43

Gene sequence:

>1908_bases
TTGCCGAAATCGGGGGTCTTCAAGGCAACCTCATCTACAATCTTCAAATACCAAGAGCACAGTATGGTCGCAAATGGCGG
GAGCCTTTACCTACAGAGCCTGATGATTCTGGGAGATGCTATCATCGCCGCGCCCCTGTTCAAACGACTGGGGCTTGGCA
CCGTGCTTGGCTATCTTGCTGCGGGCATCACCATCGGCCCGGTCGCGCGCCTCATTGCGGATGGCGAGGAATTCCTGCAT
TTTTCCGAATTGGGTGTGGTTTTCCTGCTCTTCATCATCGGGCTTGAACTGAACCCTTCACGCCTGTGGTCGCTGCGCCA
TTCGATCTTCGGTCTGGGGACCGCACAGGTACTCCTGTGCGGCGCGGCGCTGGCCGCGCTTGGCGTCTATCTTGCCGGGC
TGGATACGGAAGCCGCCATTATCATCGGCTTCGGCCTTGCCCTTTCCTCGACCGCCTTTGCCATGCAGGTTCTGGAAGAC
CGTGCCGAAACCAACCAGAAGCACGGGCAGCGCGCCTTCGCCATCCTGCTGTTCCAGGACCTGGCCATCGTGCCCATTCT
GGCAATCATTCCCGCCCTTTCGCCGAATGAACCCTCGCAGGCAAGTGCTGGCTTTCATCTTGCCATGGCCATTGCCTCGA
TTGCCGCACTGGTTATTGCCGGGCGCTATCTCATCAATCCCATGTTCCGCATCATCGCCAATACGGGTGCGCGCGAAGTG
ATGATCGCAGCCGCCCTGTTCGTGGTGCTGGGATCGGCAAGCCTCCTGCAATATGCGGGCCTTTCCATGGCCATGGGCGC
ATTCATTGCAGGTGTGCTGCTCGCCGAATCCTCCTACCGGCATGAGCTGGAAGCCGACATCGAACCGTTCCGCGGAATTT
TCCTCGGCCTGTTCTTCGTTGCAGTCGGGCTTTCGCTCAACCTCTCCGTTATCCTGGAATATTGGGCCGTGATCCTTGCC
GCCGTCCCCATTTTCATGGCGACGAAGATTGTCATCATCTACCTTCTCTGCCGCATCTTCCGCTCCAGTCACAATGATGC
GGTGCGCATTGCCTTCCTGCTGCCGCAGGGCGGTGAATTCGCTTTCGTTCTTTTTTCCGCCGCCTCTGCTGCGGCAATCA
TCTCCAGCGCACTCGGTTCCGAACTGGTCGCAGCCGTCACCGTTTCCATGGCGCTGACGCCGCTTTCCGTCGCCATCGGT
TCAAGGCTTCTCATCAAGGACAAGGCCGACGATATAATCGAGGAAAATTTCGACGGCGCGGGCTCGGATGTCCTCATGAT
CGGCTTCTCGCGTTACGGGCAGATCGCGGCACAGATTCTCCTTGCCGGCGGCATTGATGTGACTGTGATCGACAGTTCCT
CCAACCGTGTCCGCGCGGCCGGCAAGTTCGGTTTCCGCATCTATTTTGGCGATGGCACCCGCAAGGATGTGCTAGAAGCG
GCAGGCATCCGCAAGGCCAAGATCGTTGCCATTTGCACGCACAAGAAATCGACGACCAACCACATCGTCAATCTGATCCA
GTCGGAATATCCCGACGTGCGCCTGTTCGTGCGCTCCTATGACCGCGAACATACGCTGCAACTGCGCGCGCAGGGTGTGG
AATACGAGCTGCGTGAAACCTTTGAATCCGGCCTTCTGTTCGGGCAGCGCACGCTGGAGGGGCTGGGCCTTTCCGAAGCC
AATGCCTATGCGATCCGCGAAGATGTGCGCCAGCGCGACGAAGACCGGCTGCATGTGCAGGCATCAGAGGGGATCATGGC
GGGGCGCCACCTGCTTTTCAACAAGCCTGTTACGCCCGAACCGCTGGTCAAGCCCACGCGCGAGGGCCAGCGCATCGACA
AATCGGACGATGGGCAGCGCGCGCCATCCCCGCAGGAAACGGCCGCCGCACCCGTTGCCGCGGAGTAA

Upstream 100 bases:

>100_bases
TGCAAGCCCTGATCCACGGCTATCCCCAATCGGGACCTGCCAAAAAAAATCGTCAAATTGTTCGACATCGGGCTGATTGC
AGCCATATCTTGTTTCTATA

Downstream 100 bases:

>100_bases
CCCGCTTCCTTATCAAAGATCAGGCAATTTTTCCGGCAGGATCGGGGCGACCTTGCCATCGAGCGCCTGTTCGATCGCGC
GTTTCAACTCGTCCCGCACC

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MPKSGVFKATSSTIFKYQEHSMVANGGSLYLQSLMILGDAIIAAPLFKRLGLGTVLGYLAAGITIGPVARLIADGEEFLH
FSELGVVFLLFIIGLELNPSRLWSLRHSIFGLGTAQVLLCGAALAALGVYLAGLDTEAAIIIGFGLALSSTAFAMQVLED
RAETNQKHGQRAFAILLFQDLAIVPILAIIPALSPNEPSQASAGFHLAMAIASIAALVIAGRYLINPMFRIIANTGAREV
MIAAALFVVLGSASLLQYAGLSMAMGAFIAGVLLAESSYRHELEADIEPFRGIFLGLFFVAVGLSLNLSVILEYWAVILA
AVPIFMATKIVIIYLLCRIFRSSHNDAVRIAFLLPQGGEFAFVLFSAASAAAIISSALGSELVAAVTVSMALTPLSVAIG
SRLLIKDKADDIIEENFDGAGSDVLMIGFSRYGQIAAQILLAGGIDVTVIDSSSNRVRAAGKFGFRIYFGDGTRKDVLEA
AGIRKAKIVAICTHKKSTTNHIVNLIQSEYPDVRLFVRSYDREHTLQLRAQGVEYELRETFESGLLFGQRTLEGLGLSEA
NAYAIREDVRQRDEDRLHVQASEGIMAGRHLLFNKPVTPEPLVKPTREGQRIDKSDDGQRAPSPQETAAAPVAAE

Sequences:

>Translated_635_residues
MPKSGVFKATSSTIFKYQEHSMVANGGSLYLQSLMILGDAIIAAPLFKRLGLGTVLGYLAAGITIGPVARLIADGEEFLH
FSELGVVFLLFIIGLELNPSRLWSLRHSIFGLGTAQVLLCGAALAALGVYLAGLDTEAAIIIGFGLALSSTAFAMQVLED
RAETNQKHGQRAFAILLFQDLAIVPILAIIPALSPNEPSQASAGFHLAMAIASIAALVIAGRYLINPMFRIIANTGAREV
MIAAALFVVLGSASLLQYAGLSMAMGAFIAGVLLAESSYRHELEADIEPFRGIFLGLFFVAVGLSLNLSVILEYWAVILA
AVPIFMATKIVIIYLLCRIFRSSHNDAVRIAFLLPQGGEFAFVLFSAASAAAIISSALGSELVAAVTVSMALTPLSVAIG
SRLLIKDKADDIIEENFDGAGSDVLMIGFSRYGQIAAQILLAGGIDVTVIDSSSNRVRAAGKFGFRIYFGDGTRKDVLEA
AGIRKAKIVAICTHKKSTTNHIVNLIQSEYPDVRLFVRSYDREHTLQLRAQGVEYELRETFESGLLFGQRTLEGLGLSEA
NAYAIREDVRQRDEDRLHVQASEGIMAGRHLLFNKPVTPEPLVKPTREGQRIDKSDDGQRAPSPQETAAAPVAAE
>Mature_634_residues
PKSGVFKATSSTIFKYQEHSMVANGGSLYLQSLMILGDAIIAAPLFKRLGLGTVLGYLAAGITIGPVARLIADGEEFLHF
SELGVVFLLFIIGLELNPSRLWSLRHSIFGLGTAQVLLCGAALAALGVYLAGLDTEAAIIIGFGLALSSTAFAMQVLEDR
AETNQKHGQRAFAILLFQDLAIVPILAIIPALSPNEPSQASAGFHLAMAIASIAALVIAGRYLINPMFRIIANTGAREVM
IAAALFVVLGSASLLQYAGLSMAMGAFIAGVLLAESSYRHELEADIEPFRGIFLGLFFVAVGLSLNLSVILEYWAVILAA
VPIFMATKIVIIYLLCRIFRSSHNDAVRIAFLLPQGGEFAFVLFSAASAAAIISSALGSELVAAVTVSMALTPLSVAIGS
RLLIKDKADDIIEENFDGAGSDVLMIGFSRYGQIAAQILLAGGIDVTVIDSSSNRVRAAGKFGFRIYFGDGTRKDVLEAA
GIRKAKIVAICTHKKSTTNHIVNLIQSEYPDVRLFVRSYDREHTLQLRAQGVEYELRETFESGLLFGQRTLEGLGLSEAN
AYAIREDVRQRDEDRLHVQASEGIMAGRHLLFNKPVTPEPLVKPTREGQRIDKSDDGQRAPSPQETAAAPVAAE

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=365, Percent_Identity=26.3013698630137, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786232, Length=550, Percent_Identity=42.9090909090909, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1789749, Length=555, Percent_Identity=42.1621621621622, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1786685, Length=508, Percent_Identity=28.740157480315, Blast_Score=158, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 68054; Mature: 67923

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSGVFKATSSTIFKYQEHSMVANGGSLYLQSLMILGDAIIAAPLFKRLGLGTVLGYLA
CCCCCCEECCCHHHHEEHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGITIGPVARLIADGEEFLHFSELGVVFLLFIIGLELNPSRLWSLRHSIFGLGTAQVLLC
HCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHH
GAALAALGVYLAGLDTEAAIIIGFGLALSSTAFAMQVLEDRAETNQKHGQRAFAILLFQD
HHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LAIVPILAIIPALSPNEPSQASAGFHLAMAIASIAALVIAGRYLINPMFRIIANTGAREV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
MIAAALFVVLGSASLLQYAGLSMAMGAFIAGVLLAESSYRHELEADIEPFRGIFLGLFFV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHH
AVGLSLNLSVILEYWAVILAAVPIFMATKIVIIYLLCRIFRSSHNDAVRIAFLLPQGGEF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCE
AFVLFSAASAAAIISSALGSELVAAVTVSMALTPLSVAIGSRLLIKDKADDIIEENFDGA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCC
GSDVLMIGFSRYGQIAAQILLAGGIDVTVIDSSSNRVRAAGKFGFRIYFGDGTRKDVLEA
CCCEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCEEEEEEECCCCHHHHHHH
AGIRKAKIVAICTHKKSTTNHIVNLIQSEYPDVRLFVRSYDREHTLQLRAQGVEYELRET
CCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEHHHHCCCCCEEEHHHCCCCHHHHHH
FESGLLFGQRTLEGLGLSEANAYAIREDVRQRDEDRLHVQASEGIMAGRHLLFNKPVTPE
HHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHCCHHEECCCCCCC
PLVKPTREGQRIDKSDDGQRAPSPQETAAAPVAAE
CCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
PKSGVFKATSSTIFKYQEHSMVANGGSLYLQSLMILGDAIIAAPLFKRLGLGTVLGYLA
CCCCCEECCCHHHHEEHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGITIGPVARLIADGEEFLHFSELGVVFLLFIIGLELNPSRLWSLRHSIFGLGTAQVLLC
HCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHH
GAALAALGVYLAGLDTEAAIIIGFGLALSSTAFAMQVLEDRAETNQKHGQRAFAILLFQD
HHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LAIVPILAIIPALSPNEPSQASAGFHLAMAIASIAALVIAGRYLINPMFRIIANTGAREV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
MIAAALFVVLGSASLLQYAGLSMAMGAFIAGVLLAESSYRHELEADIEPFRGIFLGLFFV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHH
AVGLSLNLSVILEYWAVILAAVPIFMATKIVIIYLLCRIFRSSHNDAVRIAFLLPQGGEF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCE
AFVLFSAASAAAIISSALGSELVAAVTVSMALTPLSVAIGSRLLIKDKADDIIEENFDGA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCCC
GSDVLMIGFSRYGQIAAQILLAGGIDVTVIDSSSNRVRAAGKFGFRIYFGDGTRKDVLEA
CCCEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCEEEEEEECCCCHHHHHHH
AGIRKAKIVAICTHKKSTTNHIVNLIQSEYPDVRLFVRSYDREHTLQLRAQGVEYELRET
CCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEHHHHCCCCCEEEHHHCCCCHHHHHH
FESGLLFGQRTLEGLGLSEANAYAIREDVRQRDEDRLHVQASEGIMAGRHLLFNKPVTPE
HHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHCCHHEECCCCCCC
PLVKPTREGQRIDKSDDGQRAPSPQETAAAPVAAE
CCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]