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The map label for this gene is emrB [H]

Identifier: 163844313

GI number: 163844313

Start: 114455

End: 116044

Strand: Reverse

Name: emrB [H]

Synonym: BSUIS_B0122

Alternate gene names: 163844313

Gene position: 116044-114455 (Counterclockwise)

Preceding gene: 163844316

Following gene: 163844312

Centisome position: 8.28

GC content: 58.36

Gene sequence:

>1590_bases
ATGGCCGCAGACACCACCATAGGCACTGTCGGCGGAAAAAATCCGGCAGATGATCGCATTGATCCCAAAAAGACCATCGG
CTTTATGGCCATGGTCTTCGGCATGTTCATGGCGATCCTCGACATCCAGATTGTCTCGGCCTCGCTCTCCGAAATTCAGG
CGGGCCTTGGCGCCAGCACCGATGAAATTTCCTGGGTGCAGACCTCCTACCTGATCGCGGAAGTCATCATGATTCCGCTT
TCGGGTTTTTGGGGACGGCTTCTCTCGACGCGCGTGCTGTTTACCATTTCGGCGGCGGGCTTCACCCTTGCCAGCATGCT
CTGCGCGACGGCCACCAATATCGAGCAGATGATAGTTTACCGCGCCATTCAGGGCTTCATCGGCGGCGGTATGATCCCCA
GCGTCTTCGCGGCCGCCTTCACCATCTTTCCCCCCTCCAAGCGATCCATCGTCTCACCGATGATCGGTCTTGTGGCAACG
CTCGCGCCCACCATCGGCCCCACCATCGGCGGCTATCTCAGCCATGCCTTTTCCTGGCACTGGCTGTTCCTCGTCAATGT
CGGCCCCGGCATTCTGGTGACCATTGCCGCATGGAACCTCATCGATTTCGATGAAGGCGACCTTTCCCTTCTCGACAAGT
TCGACTGGTGGGGGCTTTTCGGCATGGCGGCCTTCCTTGGCTCCCTTGAATATGTGCTGGAGGAAGGGCCACGCAACGAC
TGGCTCCAGGACCACACGATCTTCATCATGTCGATCATCCTGACCGTCGGGGCCATCGTGTTCTTCTACCGCGTCTTCAC
AGCCGAACAGCCGATCGTGGATTTCCGCGCATTCAGGAACATGAATTTCGCCTTCGGCTCGCTCTTTTCCTTTGTGATGG
GCGTCGGCCTTTACGGACTCACCTATCTCTATCCGCTCTATCTCAGCATGATCCGCGGTTATGACGCGCTGATGATCGGC
GAAGCGCTGTTCGTCAGCGGCCTTGCCATGTTCTTCACCGCACCGGTTGCGGGCTTCCTGTCAAACCGGATGGACCCGCG
CCTCATGATGATGATCGGCTTCGTCGGCTTTGCCATCGGCACATGGCTCGTTACCGGGCTTACCGCCGACTGGGATTTCA
ATGAGCTTTTGATGCCGCAAATCCTGCGCGGCTGCTCGCTGATGCTGTGCATGGTGCCGATCAACAATCTGGCGCTCGGT
ACATTGCCGCCCTCGCTGATGAAGAACGCCTCCGGCCTTTTCAACCTCACCCGCAATCTGGGCGGCGCGGTCGGCCTGGC
CGTCATCAATACGATCCTGACACGCCGCGGCGACATGCATTATGCGCGCCTGGCCGAACATGTGCGCAACGGCAATGCCG
AAGCTGAAAACATGATTGCAAACCTGACAGCCAAGTATAATGCCGCCGGAATGGATGGGGCCTCGATAGCCATAGCCAAG
CTTTCCGGCATGGTGCGCCAGCAGGCGACGCTTCTGTCTTTCATCGACGTCTTCTTCATTCTGACGATGATGTTCTGCTC
GCTTGCCATCTGCGCGATGCTTCTGCGCAAGCCCTCACCGGCAGCAGGCGGTGGTGGCGGCGGGCATTAA

Upstream 100 bases:

>100_bases
CAAACTGACTGGAACAGCAAAACATTCAGAACCGAATGGCGAAGCCTGAAAGCCTGAACGGGCCGGGCATTGCCGTTTTC
TCTTCCCGGAGCGCGTCAAT

Downstream 100 bases:

>100_bases
AACATACCTCCCAAAAGCGGGGCCCGGTTTTGGGAATAAGATGCGCGCGATTCGCAACCGAAGTGTTTCATCCGAAAGCA
GAAAGGTTCACTGCCTTTCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MAADTTIGTVGGKNPADDRIDPKKTIGFMAMVFGMFMAILDIQIVSASLSEIQAGLGASTDEISWVQTSYLIAEVIMIPL
SGFWGRLLSTRVLFTISAAGFTLASMLCATATNIEQMIVYRAIQGFIGGGMIPSVFAAAFTIFPPSKRSIVSPMIGLVAT
LAPTIGPTIGGYLSHAFSWHWLFLVNVGPGILVTIAAWNLIDFDEGDLSLLDKFDWWGLFGMAAFLGSLEYVLEEGPRND
WLQDHTIFIMSIILTVGAIVFFYRVFTAEQPIVDFRAFRNMNFAFGSLFSFVMGVGLYGLTYLYPLYLSMIRGYDALMIG
EALFVSGLAMFFTAPVAGFLSNRMDPRLMMMIGFVGFAIGTWLVTGLTADWDFNELLMPQILRGCSLMLCMVPINNLALG
TLPPSLMKNASGLFNLTRNLGGAVGLAVINTILTRRGDMHYARLAEHVRNGNAEAENMIANLTAKYNAAGMDGASIAIAK
LSGMVRQQATLLSFIDVFFILTMMFCSLAICAMLLRKPSPAAGGGGGGH

Sequences:

>Translated_529_residues
MAADTTIGTVGGKNPADDRIDPKKTIGFMAMVFGMFMAILDIQIVSASLSEIQAGLGASTDEISWVQTSYLIAEVIMIPL
SGFWGRLLSTRVLFTISAAGFTLASMLCATATNIEQMIVYRAIQGFIGGGMIPSVFAAAFTIFPPSKRSIVSPMIGLVAT
LAPTIGPTIGGYLSHAFSWHWLFLVNVGPGILVTIAAWNLIDFDEGDLSLLDKFDWWGLFGMAAFLGSLEYVLEEGPRND
WLQDHTIFIMSIILTVGAIVFFYRVFTAEQPIVDFRAFRNMNFAFGSLFSFVMGVGLYGLTYLYPLYLSMIRGYDALMIG
EALFVSGLAMFFTAPVAGFLSNRMDPRLMMMIGFVGFAIGTWLVTGLTADWDFNELLMPQILRGCSLMLCMVPINNLALG
TLPPSLMKNASGLFNLTRNLGGAVGLAVINTILTRRGDMHYARLAEHVRNGNAEAENMIANLTAKYNAAGMDGASIAIAK
LSGMVRQQATLLSFIDVFFILTMMFCSLAICAMLLRKPSPAAGGGGGGH
>Mature_528_residues
AADTTIGTVGGKNPADDRIDPKKTIGFMAMVFGMFMAILDIQIVSASLSEIQAGLGASTDEISWVQTSYLIAEVIMIPLS
GFWGRLLSTRVLFTISAAGFTLASMLCATATNIEQMIVYRAIQGFIGGGMIPSVFAAAFTIFPPSKRSIVSPMIGLVATL
APTIGPTIGGYLSHAFSWHWLFLVNVGPGILVTIAAWNLIDFDEGDLSLLDKFDWWGLFGMAAFLGSLEYVLEEGPRNDW
LQDHTIFIMSIILTVGAIVFFYRVFTAEQPIVDFRAFRNMNFAFGSLFSFVMGVGLYGLTYLYPLYLSMIRGYDALMIGE
ALFVSGLAMFFTAPVAGFLSNRMDPRLMMMIGFVGFAIGTWLVTGLTADWDFNELLMPQILRGCSLMLCMVPINNLALGT
LPPSLMKNASGLFNLTRNLGGAVGLAVINTILTRRGDMHYARLAEHVRNGNAEAENMIANLTAKYNAAGMDGASIAIAKL
SGMVRQQATLLSFIDVFFILTMMFCSLAICAMLLRKPSPAAGGGGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=463, Percent_Identity=28.9416846652268, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1788710, Length=499, Percent_Identity=28.8577154308617, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1790195, Length=416, Percent_Identity=25.9615384615385, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081983, Length=433, Percent_Identity=26.3279445727483, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788392, Length=413, Percent_Identity=24.6973365617433, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57194; Mature: 57063

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADTTIGTVGGKNPADDRIDPKKTIGFMAMVFGMFMAILDIQIVSASLSEIQAGLGAST
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEISWVQTSYLIAEVIMIPLSGFWGRLLSTRVLFTISAAGFTLASMLCATATNIEQMIVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAIQGFIGGGMIPSVFAAAFTIFPPSKRSIVSPMIGLVATLAPTIGPTIGGYLSHAFSWH
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEE
WLFLVNVGPGILVTIAAWNLIDFDEGDLSLLDKFDWWGLFGMAAFLGSLEYVLEEGPRND
EEEEEECCCCCEEEEEHHCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
WLQDHTIFIMSIILTVGAIVFFYRVFTAEQPIVDFRAFRNMNFAFGSLFSFVMGVGLYGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TYLYPLYLSMIRGYDALMIGEALFVSGLAMFFTAPVAGFLSNRMDPRLMMMIGFVGFAIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TWLVTGLTADWDFNELLMPQILRGCSLMLCMVPINNLALGTLPPSLMKNASGLFNLTRNL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHHHHH
GGAVGLAVINTILTRRGDMHYARLAEHVRNGNAEAENMIANLTAKYNAAGMDGASIAIAK
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
LSGMVRQQATLLSFIDVFFILTMMFCSLAICAMLLRKPSPAAGGGGGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AADTTIGTVGGKNPADDRIDPKKTIGFMAMVFGMFMAILDIQIVSASLSEIQAGLGAST
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEISWVQTSYLIAEVIMIPLSGFWGRLLSTRVLFTISAAGFTLASMLCATATNIEQMIVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAIQGFIGGGMIPSVFAAAFTIFPPSKRSIVSPMIGLVATLAPTIGPTIGGYLSHAFSWH
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEE
WLFLVNVGPGILVTIAAWNLIDFDEGDLSLLDKFDWWGLFGMAAFLGSLEYVLEEGPRND
EEEEEECCCCCEEEEEHHCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
WLQDHTIFIMSIILTVGAIVFFYRVFTAEQPIVDFRAFRNMNFAFGSLFSFVMGVGLYGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TYLYPLYLSMIRGYDALMIGEALFVSGLAMFFTAPVAGFLSNRMDPRLMMMIGFVGFAIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TWLVTGLTADWDFNELLMPQILRGCSLMLCMVPINNLALGTLPPSLMKNASGLFNLTRNL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHHHHH
GGAVGLAVINTILTRRGDMHYARLAEHVRNGNAEAENMIANLTAKYNAAGMDGASIAIAK
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
LSGMVRQQATLLSFIDVFFILTMMFCSLAICAMLLRKPSPAAGGGGGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]