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The map label for this gene is mltB [H]

Identifier: 163844269

GI number: 163844269

Start: 67794

End: 69020

Strand: Reverse

Name: mltB [H]

Synonym: BSUIS_B0075

Alternate gene names: 163844269

Gene position: 69020-67794 (Counterclockwise)

Preceding gene: 163844271

Following gene: 163844267

Centisome position: 4.93

GC content: 59.25

Gene sequence:

>1227_bases
ATGCTGCGATTTCCATTCACTCCGGAAAAGAAGCTGCGTATCAAGATCGCCGCAACGGTGGCGCTTGCCATAATCGGAGC
CAGCCTTTCGGCCGGCACGGCGCTGGCCGACGCCGGATTTCGCCAGTGGATCGCAAATTTCCGTCCCGTCGCGATCAAGA
GCGGCATTGCACCCTCCACATTCGACCGGGCTTTCAGGGGCGTCAACGAACCCGACCCGGTGGTGTTGGAGAAGGCGCGG
TACCAGCCGGAATTTACCGAACCTGTCTGGAATTATATCGACAACCGCGTCAACGAACAATCCGTCGCGGTGGGTCAGAA
TATGGCGCGCAAATGGGGGCAGTGGCTCCAGCGCATCGAGCATCACTATTCGGTCGATCGCCATATCCTGCTCGCGATCT
GGTCAATGGAAAGCAATTACGGCGAGATTCTCAAGCGTGACGATGTAATGCGCGATGCGATCCGTTCGCTTGCCACACTT
GCCTATGCCGATGCCCGGCGCGCCAAATATGGCCGCACACAACTCATCGCCGCAATGAAGATTCTCCAGACCGGCGATAT
CGAGCGCGGCCATCTTTCCGGCTCCTGGGCCGGGGCGCTCGGCCATACGCAGTTCATTCCCACCAGCTATCAGGCCTATA
CGGTCGATATGGATGGCAACGGCAAGCGCGATATCTGGAATTCGGTTCCCGATGCGCTTGGCACGGCGGCCAACCTGCTC
CATCGCAATGGCTGGCAACCAGGCCGCACATGGGGCTATGAGGTGATGCTCCCGGCGGGGCGCAAATTCCCGTCCGGTTC
GCTCACCATCGCCGAATGGCAGCAAATCGGCCTCGTGCGCGCCAATGGCCGTGCCTTCCCGGACCCTCACGAAAAAGCGA
CACTGAAAGTGCCGGATGGCCATCAGGGACCAGCCTTCCTGATGACAAAGAATTTCTTTGTTCTGAAGCGCTATAACAAT
GCCGATAAATATGCGCTGGCCGTCGGCCTGCTTGCAGACCGTATCGGCGGCTATCAGGGCTTGCGGCAGGACTGGAACCG
TCCATTCACCCCGATATCGATGAATGAGCGGCTGGAACTGCAAACGCACCTCAAGGCGCTCGGCTATTATGACGGCAAGA
TCGGCGCAACCTCACGCGCTGCCATAGAAGCCTTCCAGCAGCGCAATGGCCTTGAGCCGGATGGGCATCCGAGCAAGGAA
GTCCTTTCCGTCCTGCGTCGGCGCTGA

Upstream 100 bases:

>100_bases
TTTTCAACATAGGATTGTATCGTAGACTTCCTGCAACCATGTAAGCGCGATGATGAATGGCGAAATGGACAGGAAGACGA
TTGCAGAAGGGAAACACAGA

Downstream 100 bases:

>100_bases
TGCCGCCTTGTTCACCGGCTAGCTGAAATCCAGGCGTTGAGATCGCCATGACAGGCATATTTCAACGCGACATATTCATT
TGCTACCGCATTTCGACCCT

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MLRFPFTPEKKLRIKIAATVALAIIGASLSAGTALADAGFRQWIANFRPVAIKSGIAPSTFDRAFRGVNEPDPVVLEKAR
YQPEFTEPVWNYIDNRVNEQSVAVGQNMARKWGQWLQRIEHHYSVDRHILLAIWSMESNYGEILKRDDVMRDAIRSLATL
AYADARRAKYGRTQLIAAMKILQTGDIERGHLSGSWAGALGHTQFIPTSYQAYTVDMDGNGKRDIWNSVPDALGTAANLL
HRNGWQPGRTWGYEVMLPAGRKFPSGSLTIAEWQQIGLVRANGRAFPDPHEKATLKVPDGHQGPAFLMTKNFFVLKRYNN
ADKYALAVGLLADRIGGYQGLRQDWNRPFTPISMNERLELQTHLKALGYYDGKIGATSRAAIEAFQQRNGLEPDGHPSKE
VLSVLRRR

Sequences:

>Translated_408_residues
MLRFPFTPEKKLRIKIAATVALAIIGASLSAGTALADAGFRQWIANFRPVAIKSGIAPSTFDRAFRGVNEPDPVVLEKAR
YQPEFTEPVWNYIDNRVNEQSVAVGQNMARKWGQWLQRIEHHYSVDRHILLAIWSMESNYGEILKRDDVMRDAIRSLATL
AYADARRAKYGRTQLIAAMKILQTGDIERGHLSGSWAGALGHTQFIPTSYQAYTVDMDGNGKRDIWNSVPDALGTAANLL
HRNGWQPGRTWGYEVMLPAGRKFPSGSLTIAEWQQIGLVRANGRAFPDPHEKATLKVPDGHQGPAFLMTKNFFVLKRYNN
ADKYALAVGLLADRIGGYQGLRQDWNRPFTPISMNERLELQTHLKALGYYDGKIGATSRAAIEAFQQRNGLEPDGHPSKE
VLSVLRRR
>Mature_408_residues
MLRFPFTPEKKLRIKIAATVALAIIGASLSAGTALADAGFRQWIANFRPVAIKSGIAPSTFDRAFRGVNEPDPVVLEKAR
YQPEFTEPVWNYIDNRVNEQSVAVGQNMARKWGQWLQRIEHHYSVDRHILLAIWSMESNYGEILKRDDVMRDAIRSLATL
AYADARRAKYGRTQLIAAMKILQTGDIERGHLSGSWAGALGHTQFIPTSYQAYTVDMDGNGKRDIWNSVPDALGTAANLL
HRNGWQPGRTWGYEVMLPAGRKFPSGSLTIAEWQQIGLVRANGRAFPDPHEKATLKVPDGHQGPAFLMTKNFFVLKRYNN
ADKYALAVGLLADRIGGYQGLRQDWNRPFTPISMNERLELQTHLKALGYYDGKIGATSRAAIEAFQQRNGLEPDGHPSKE
VLSVLRRR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=237, Percent_Identity=29.535864978903, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45740; Mature: 45740

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFPFTPEKKLRIKIAATVALAIIGASLSAGTALADAGFRQWIANFRPVAIKSGIAPST
CCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHH
FDRAFRGVNEPDPVVLEKARYQPEFTEPVWNYIDNRVNEQSVAVGQNMARKWGQWLQRIE
HHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
HHYSVDRHILLAIWSMESNYGEILKRDDVMRDAIRSLATLAYADARRAKYGRTQLIAAMK
HHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ILQTGDIERGHLSGSWAGALGHTQFIPTSYQAYTVDMDGNGKRDIWNSVPDALGTAANLL
HHHCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRNGWQPGRTWGYEVMLPAGRKFPSGSLTIAEWQQIGLVRANGRAFPDPHEKATLKVPDG
HHCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHCCEEEECCCCCCCCCCCEEEECCCC
HQGPAFLMTKNFFVLKRYNNADKYALAVGLLADRIGGYQGLRQDWNRPFTPISMNERLEL
CCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHH
QTHLKALGYYDGKIGATSRAAIEAFQQRNGLEPDGHPSKEVLSVLRRR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLRFPFTPEKKLRIKIAATVALAIIGASLSAGTALADAGFRQWIANFRPVAIKSGIAPST
CCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHH
FDRAFRGVNEPDPVVLEKARYQPEFTEPVWNYIDNRVNEQSVAVGQNMARKWGQWLQRIE
HHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
HHYSVDRHILLAIWSMESNYGEILKRDDVMRDAIRSLATLAYADARRAKYGRTQLIAAMK
HHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ILQTGDIERGHLSGSWAGALGHTQFIPTSYQAYTVDMDGNGKRDIWNSVPDALGTAANLL
HHHCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRNGWQPGRTWGYEVMLPAGRKFPSGSLTIAEWQQIGLVRANGRAFPDPHEKATLKVPDG
HHCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHCCEEEECCCCCCCCCCCEEEECCCC
HQGPAFLMTKNFFVLKRYNNADKYALAVGLLADRIGGYQGLRQDWNRPFTPISMNERLEL
CCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHH
QTHLKALGYYDGKIGATSRAAIEAFQQRNGLEPDGHPSKEVLSVLRRR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]