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The map label for this gene is rihA [H]

Identifier: 163844205

GI number: 163844205

Start: 3110

End: 4108

Strand: Reverse

Name: rihA [H]

Synonym: BSUIS_B0008

Alternate gene names: 163844205

Gene position: 4108-3110 (Counterclockwise)

Preceding gene: 163844206

Following gene: 163844201

Centisome position: 0.29

GC content: 56.36

Gene sequence:

>999_bases
TTGTTGAGCAAACATGCAGTATCATGCGCAATACCCTCCCAACGAACTCATCAGGAAACCACCATGGCCCGTAAAATCAT
TATCGATACCGATCCCGGTCAGGACGATGCCGTCGCCATTTTGCTGGCGCTTGCAAGCCCTGAACTCGACATTCTCGGCA
TTACCGCCGTTGCAGGAAATGGCCCGCTGGCGCGCACAGAAGTCAATGCCCGCACGGTCTGCGAAGTGGCGAAAAAGCCG
GACACAAAAGTATTTGCAGGCTCCATCCGCCCATTGGTGCGCCCGCTTGTCACCGCTGAAAACGTTCATGGCAAGACCGG
CCTCGATGGCTATGACCTGCCCGCCCCCACCATGCCGCTTCAAGCCCAGCATGGCGTTGATTTCATCATTGAAACACTGA
TGAAGGAAGAACCGGGCACCGTCACACTCTGCCCGATCGGACCGCTGACCAACATCGCTTCCGCGCTGATCCGCGAGCCG
AAGATTGCCGGGCGCGTCAAGGAAATCGTGCTGATGGGCGGCGGCTATTTCGAGGGCGGCAACATCACGCCATCGGCGGA
ATTCAACATCTATGTCGATCCGCATGCCGCATCGGTTGTCTTCAGCTCCGGCATCAAGATCACCATGTTGCCGCTCGATG
TCACGCACAAGGTTCTCACCACTGAAAAGCGCATCGCCGCCATTCGCAACATAGGCAGCCATGTCGGCGAAGTGGTCGCG
GCCTGGCTTGAGTTTTTCGAGCGTTATGATGAAGCCAAATACGGCACCGATGGTGCCCCACTGCATGACCCGAACGTCAT
CGCCTATCTCATCAAGCCGGAGCTTTATTCAGGCCGTGCATGCAATGTGGAAATCGAGATCAATTTGGAACTGACCATCG
GCGAAACAGTCGTTGACTGGTGGGGGGTGACAGACCGTTCGAAGAATGCCTTCTTCATCAAGGATGTGGATGCCGATGGC
TTCTTCAGCCTGCTGACGGAGCGTCTGGCGACGCTTTGA

Upstream 100 bases:

>100_bases
ATCGTGCTGACGGTCCATGCCGCGCGGCGCCAGCGGCGCGCACGGCGGCAGATCACGCCCGAGACACAAGAGACATAGGC
GCCGTCATGACAGCGCCCCC

Downstream 100 bases:

>100_bases
CCGGTATTGGCTGGTTCACAGAAACGCCGCGCAGGCCAAACCTTGCACGGCTTTTCTTTTGAAACAAGCTGGAAGCTCAA
GCGCGCCCGATGAGTTCCTC

Product: hypothetical protein

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLSKHAVSCAIPSQRTHQETTMARKIIIDTDPGQDDAVAILLALASPELDILGITAVAGNGPLARTEVNARTVCEVAKKP
DTKVFAGSIRPLVRPLVTAENVHGKTGLDGYDLPAPTMPLQAQHGVDFIIETLMKEEPGTVTLCPIGPLTNIASALIREP
KIAGRVKEIVLMGGGYFEGGNITPSAEFNIYVDPHAASVVFSSGIKITMLPLDVTHKVLTTEKRIAAIRNIGSHVGEVVA
AWLEFFERYDEAKYGTDGAPLHDPNVIAYLIKPELYSGRACNVEIEINLELTIGETVVDWWGVTDRSKNAFFIKDVDADG
FFSLLTERLATL

Sequences:

>Translated_332_residues
MLSKHAVSCAIPSQRTHQETTMARKIIIDTDPGQDDAVAILLALASPELDILGITAVAGNGPLARTEVNARTVCEVAKKP
DTKVFAGSIRPLVRPLVTAENVHGKTGLDGYDLPAPTMPLQAQHGVDFIIETLMKEEPGTVTLCPIGPLTNIASALIREP
KIAGRVKEIVLMGGGYFEGGNITPSAEFNIYVDPHAASVVFSSGIKITMLPLDVTHKVLTTEKRIAAIRNIGSHVGEVVA
AWLEFFERYDEAKYGTDGAPLHDPNVIAYLIKPELYSGRACNVEIEINLELTIGETVVDWWGVTDRSKNAFFIKDVDADG
FFSLLTERLATL
>Mature_332_residues
MLSKHAVSCAIPSQRTHQETTMARKIIIDTDPGQDDAVAILLALASPELDILGITAVAGNGPLARTEVNARTVCEVAKKP
DTKVFAGSIRPLVRPLVTAENVHGKTGLDGYDLPAPTMPLQAQHGVDFIIETLMKEEPGTVTLCPIGPLTNIASALIREP
KIAGRVKEIVLMGGGYFEGGNITPSAEFNIYVDPHAASVVFSSGIKITMLPLDVTHKVLTTEKRIAAIRNIGSHVGEVVA
AWLEFFERYDEAKYGTDGAPLHDPNVIAYLIKPELYSGRACNVEIEINLELTIGETVVDWWGVTDRSKNAFFIKDVDADG
FFSLLTERLATL

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=45.3074433656958, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1788486, Length=304, Percent_Identity=42.1052631578947, Blast_Score=237, Evalue=7e-64,
Organism=Escherichia coli, GI1786213, Length=309, Percent_Identity=37.5404530744337, Blast_Score=158, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI32565474, Length=199, Percent_Identity=35.1758793969849, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17565698, Length=293, Percent_Identity=27.6450511945392, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=264, Percent_Identity=35.2272727272727, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24641839, Length=191, Percent_Identity=31.413612565445, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583915, Length=190, Percent_Identity=32.1052631578947, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24641837, Length=337, Percent_Identity=28.1899109792285, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 35883; Mature: 35883

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKHAVSCAIPSQRTHQETTMARKIIIDTDPGQDDAVAILLALASPELDILGITAVAGN
CCCCCEEEEECCCCCCHHHHHHEEEEEEECCCCCCHHEEEEEECCCCCEEEEEEEEEECC
GPLARTEVNARTVCEVAKKPDTKVFAGSIRPLVRPLVTAENVHGKTGLDGYDLPAPTMPL
CCEEEEECCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QAQHGVDFIIETLMKEEPGTVTLCPIGPLTNIASALIREPKIAGRVKEIVLMGGGYFEGG
CCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCHHCCEEEEEEECCCEEECC
NITPSAEFNIYVDPHAASVVFSSGIKITMLPLDVTHKVLTTEKRIAAIRNIGSHVGEVVA
CCCCCCEEEEEECCCHHEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLEFFERYDEAKYGTDGAPLHDPNVIAYLIKPELYSGRACNVEIEINLELTIGETVVDW
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEEEEECCEEHHH
WGVTDRSKNAFFIKDVDADGFFSLLTERLATL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLSKHAVSCAIPSQRTHQETTMARKIIIDTDPGQDDAVAILLALASPELDILGITAVAGN
CCCCCEEEEECCCCCCHHHHHHEEEEEEECCCCCCHHEEEEEECCCCCEEEEEEEEEECC
GPLARTEVNARTVCEVAKKPDTKVFAGSIRPLVRPLVTAENVHGKTGLDGYDLPAPTMPL
CCEEEEECCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QAQHGVDFIIETLMKEEPGTVTLCPIGPLTNIASALIREPKIAGRVKEIVLMGGGYFEGG
CCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCHHCCEEEEEEECCCEEECC
NITPSAEFNIYVDPHAASVVFSSGIKITMLPLDVTHKVLTTEKRIAAIRNIGSHVGEVVA
CCCCCCEEEEEECCCHHEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLEFFERYDEAKYGTDGAPLHDPNVIAYLIKPELYSGRACNVEIEINLELTIGETVVDW
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEEEEECCEEHHH
WGVTDRSKNAFFIKDVDADGFFSLLTERLATL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA