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The map label for this gene is gcvA

Identifier: 16273646

GI number: 16273646

Start: 1260108

End: 1261001

Strand: Direct

Name: gcvA

Synonym: HI1194m

Alternate gene names: 16273646

Gene position: 1260108-1261001 (Clockwise)

Preceding gene: 16273114

Following gene: 16273647

Centisome position: 68.85

GC content: 34.71

Gene sequence:

>894_bases
ATGTATAAACGTTTGCCACCATTGAATTCACTAAAATCTTTTGAGTCGGCTGCTCGTTATTTAAGTTTTACTAAAGCAGC
AGATGAGCTTTGTGTTACACAGGCGGCGGTTAGTCATCAAATTAAATTATTAGAAGANTTTTTAGGGATTGATTTATTTA
AGCGAAAGAATCGTTCTTTAGAATTAACGGAGCTTGGTAAAGCTTATTTTGTTGATATTAATAAAATTCTGCGTCGCTTA
AATGAAGCAACAGAGCGATTATTAACGTTAAAAACGGATCCGCATTTAAATATTAGCGTGCCACAAACTTTTGGTATCCA
ATGGTTAGTGCCACATTTAAGTGAATTTAATCAGCTTTATCCGCAAATTGAGGTTCGTTTAACAGGCGTGGATCAAGATG
AAGGTTTGTTAAATAAAGAAATTGATCTTGCCATTTATTATGGTTTGGGAAATTGGCAGAATCTTCAAGTAGATCGTCTT
TGCGAAGAAAATTTATTGATTTTAGCCTCACCTGAATTGCTAGCAGAAAATCCAATTATTCAGCCTGAAGATTTAAAAAA
ACACACATTAATTCACATTCATACCTGTGATAATTGGCAAGCGATGGCAAATCATTTACAGTTAGATGATTTAAATATTC
AGCAAGGACCGTTATTTAGCCATACATTTATGGCGTTACAGGCGGCTATTCACGGGCAAGGCATTGTATTAGCTAATCGC
TTATTAGCCTTGCAAGAAATTGAAAATGGATCATTGCAAGCAGTGTTGCCGACTAATTTGCCTGATCCAAAATCATTTTA
TGTGGTCAATCATCTTGATCGCCTTGATGACCAAAAAATTCAAGCATTCCGTCAGTGGATTATTAACTCAATTAAACAAG
AAGAAAATGAATAA

Upstream 100 bases:

>100_bases
GGAAAGGACAAGTGACGTTTTTTTATTTGAGGTATTAGAAAAACTAATTTGAAATAAATCTTGTCCATTATAGACGAAAA
TTTTATAAATGGAAATACTT

Downstream 100 bases:

>100_bases
ACTCGCATTATATTGTCGCCCAGGTTTTGAGAAAGAAGTCGCCGCAGAGATTACCGATCAAGCAAGTCATTTAGGTGTAT
TTGGTTTTGCTCGAGTGCAA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MYKRLPPLNSLKSFESAARYLSFTKAADELCVTQAAVSHQIKLLEXFLGIDLFKRKNRSLELTELGKAYFVDINKILRRL
NEATERLLTLKTDPHLNISVPQTFGIQWLVPHLSEFNQLYPQIEVRLTGVDQDEGLLNKEIDLAIYYGLGNWQNLQVDRL
CEENLLILASPELLAENPIIQPEDLKKHTLIHIHTCDNWQAMANHLQLDDLNIQQGPLFSHTFMALQAAIHGQGIVLANR
LLALQEIENGSLQAVLPTNLPDPKSFYVVNHLDRLDDQKIQAFRQWIINSIKQEENE

Sequences:

>Translated_297_residues
MYKRLPPLNSLKSFESAARYLSFTKAADELCVTQAAVSHQIKLLEXFLGIDLFKRKNRSLELTELGKAYFVDINKILRRL
NEATERLLTLKTDPHLNISVPQTFGIQWLVPHLSEFNQLYPQIEVRLTGVDQDEGLLNKEIDLAIYYGLGNWQNLQVDRL
CEENLLILASPELLAENPIIQPEDLKKHTLIHIHTCDNWQAMANHLQLDDLNIQQGPLFSHTFMALQAAIHGQGIVLANR
LLALQEIENGSLQAVLPTNLPDPKSFYVVNHLDRLDDQKIQAFRQWIINSIKQEENE
>Mature_297_residues
MYKRLPPLNSLKSFESAARYLSFTKAADELCVTQAAVSHQIKLLEXFLGIDLFKRKNRSLELTELGKAYFVDINKILRRL
NEATERLLTLKTDPHLNISVPQTFGIQWLVPHLSEFNQLYPQIEVRLTGVDQDEGLLNKEIDLAIYYGLGNWQNLQVDRL
CEENLLILASPELLAENPIIQPEDLKKHTLIHIHTCDNWQAMANHLQLDDLNIQQGPLFSHTFMALQAAIHGQGIVLANR
LLALQEIENGSLQAVLPTNLPDPKSFYVVNHLDRLDDQKIQAFRQWIINSIKQEENE

Specific function: Not known, the gcv operon regulated by the E.coli homolog does not exist in H.influenzae, so it probably acts as a transcriptional regulator on some other operon

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=52, Blast_Score=303, Evalue=8e-84,
Organism=Escherichia coli, GI1786448, Length=295, Percent_Identity=32.5423728813559, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=29.0102389078498, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=27.6450511945392, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=26.5993265993266, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081978, Length=261, Percent_Identity=30.6513409961686, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=25.1724137931034, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI157672245, Length=254, Percent_Identity=27.1653543307087, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790262, Length=188, Percent_Identity=32.4468085106383, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082132, Length=161, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789440, Length=245, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787128, Length=269, Percent_Identity=26.3940520446097, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCVA_HAEIN (P45099)

Other databases:

- EMBL:   L42023
- PIR:   B64189
- RefSeq:   NP_439350.1
- ProteinModelPortal:   P45099
- GeneID:   950145
- GenomeReviews:   L42023_GR
- NMPDR:   fig|71421.1.peg.1141
- TIGR:   HI_1194
- HOGENOM:   HBG685425
- OMA:   LIHVHTR
- ProtClustDB:   PRK11139
- BioCyc:   HINF71421:HI_1194-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33987; Mature: 33987

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKRLPPLNSLKSFESAARYLSFTKAADELCVTQAAVSHQIKLLEXFLGIDLFKRKNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ELTELGKAYFVDINKILRRLNEATERLLTLKTDPHLNISVPQTFGIQWLVPHLSEFNQLY
EHHHCCCCEEEEHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCEEHHHHHHHHHHHC
PQIEVRLTGVDQDEGLLNKEIDLAIYYGLGNWQNLQVDRLCEENLLILASPELLAENPII
CEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCEEEEECCHHHHCCCCC
QPEDLKKHTLIHIHTCDNWQAMANHLQLDDLNIQQGPLFSHTFMALQAAIHGQGIVLANR
CCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
LLALQEIENGSLQAVLPTNLPDPKSFYVVNHLDRLDDQKIQAFRQWIINSIKQEENE
HHHHHHCCCCCEEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYKRLPPLNSLKSFESAARYLSFTKAADELCVTQAAVSHQIKLLEXFLGIDLFKRKNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ELTELGKAYFVDINKILRRLNEATERLLTLKTDPHLNISVPQTFGIQWLVPHLSEFNQLY
EHHHCCCCEEEEHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCEEHHHHHHHHHHHC
PQIEVRLTGVDQDEGLLNKEIDLAIYYGLGNWQNLQVDRLCEENLLILASPELLAENPII
CEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCEEEEECCHHHHCCCCC
QPEDLKKHTLIHIHTCDNWQAMANHLQLDDLNIQQGPLFSHTFMALQAAIHGQGIVLANR
CCCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
LLALQEIENGSLQAVLPTNLPDPKSFYVVNHLDRLDDQKIQAFRQWIINSIKQEENE
HHHHHHCCCCCEEEEECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800